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package stream;
import java.io.IOException;
import java.io.OutputStream;
import java.io.PrintWriter;
import java.util.ArrayList;
import java.util.concurrent.ArrayBlockingQueue;
import dna.Data;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import shared.KillSwitch;
import shared.Shared;
import shared.Tools;
import structures.ByteBuilder;
public abstract class ReadStreamWriter extends Thread {
/*--------------------------------------------------------------*/
/*---------------- Initialization ----------------*/
/*--------------------------------------------------------------*/
protected ReadStreamWriter(FileFormat ff, String qfname_, boolean read1_, int bufferSize, CharSequence header,
boolean makeWriter, boolean buffered, boolean useSharedHeader){
// assert(false) : useSharedHeader+", "+header;
assert(ff!=null);
assert(ff.write()) : "FileFormat is not in write mode for "+ff.name();
assert(!ff.text() && !ff.unknownFormat()) : "Unknown format for "+ff;
OUTPUT_FASTQ=ff.fastq();
OUTPUT_FASTA=ff.fasta();
OUTPUT_FASTR=ff.fastr();
// boolean bread=(ext==TestFormat.txt);
OUTPUT_SAM=ff.samOrBam();
OUTPUT_BAM=ff.bam();
OUTPUT_ATTACHMENT=ff.attachment();
OUTPUT_HEADER=ff.header();
OUTPUT_ONELINE=ff.oneline();
SITES_ONLY=ff.sites();
OUTPUT_STANDARD_OUT=ff.stdio();
FASTA_WRAP=Shared.FASTA_WRAP;
assert(((OUTPUT_SAM ? 1 : 0)+(OUTPUT_FASTQ ? 1 : 0)+(OUTPUT_FASTA ? 1 : 0)+(OUTPUT_ATTACHMENT ? 1 : 0)+
(OUTPUT_HEADER ? 1 : 0)+(OUTPUT_ONELINE ? 1 : 0)+(SITES_ONLY ? 1 : 0))<=1) :
OUTPUT_SAM+", "+SITES_ONLY+", "+OUTPUT_FASTQ+", "+OUTPUT_FASTA+", "+OUTPUT_ATTACHMENT+", "+OUTPUT_HEADER+", "+OUTPUT_ONELINE;
fname=ff.name();
qfname=qfname_;
read1=read1_;
allowSubprocess=ff.allowSubprocess();
// assert(fname==null || (fname.contains(".sam") || fname.contains(".bam"))==OUTPUT_SAM) : "Outfile name and sam output mode flag disagree: "+fname;
assert(read1 || !OUTPUT_SAM) : "Attempting to output paired reads to different sam files.";
if(qfname==null){
myQOutstream=null;
myQWriter=null;
}else{
myQOutstream=ReadWrite.getOutputStream(qfname, (ff==null ? false : ff.append()), buffered, allowSubprocess);
myQWriter=(makeWriter ? new PrintWriter(myQOutstream) : null);
}
if(header==null){header=HEADER;} //new line; test.
if(fname==null && !OUTPUT_STANDARD_OUT){
myOutstream=null;
myWriter=null;
}else{
if(OUTPUT_STANDARD_OUT){myOutstream=System.out;}
else if(!OUTPUT_BAM || !(Data.SAMTOOLS() /*|| Data.SAMBAMBA()*/) /*|| !Data.SH()*/){
myOutstream=ReadWrite.getOutputStream(ff, buffered);
}else{
if(!allowSubprocess){System.err.println("Warning! Spawning a samtools process when allowSubprocess="+allowSubprocess);}
String command;
if(Data.SAMTOOLS()){
command="samtools view -S -b -h - ";
int threads=Tools.min(ReadWrite.MAX_ZIP_THREADS(), Shared.threads(), ReadWrite.MAX_SAMTOOLS_THREADS);
if(threads>1){
command="samtools view -S -b -h -@ "+threads+" - ";
}
}else{
command= "sambamba view -S -f bam -h "; //Sambamba does not support stdin
}
myOutstream=ReadWrite.getOutputStreamFromProcess(fname, command, true, ff.append(), true, true);
}
myWriter=(makeWriter ? new PrintWriter(myOutstream) : null);
final boolean supressHeader=(NO_HEADER || (ff.append() && ff.exists()));
final boolean supressHeaderSequences=(NO_HEADER_SEQUENCES);
// assert(false) : ff.append()+", "+ff.exists();
if(header!=null && !supressHeader){
if(myWriter!=null){
myWriter.println(header);
}else{
byte[] temp=new byte[header.length()];
for(int i=0; i<temp.length; i++){temp[i]=(byte)header.charAt(i);}
try {
myOutstream.write(temp);
} catch (IOException e) {
// TODO Auto-generated catch block
e.printStackTrace();
}
}
}else if(OUTPUT_SAM && !supressHeader){
if(useSharedHeader){
// assert(false);
ArrayList<byte[]> list=SamReadInputStream.getSharedHeader(true);
if(list==null){
System.err.println("Header was null.");
}else{
try {
if(supressHeaderSequences){
for(byte[] line : list){
boolean sq=(line!=null && line.length>2 && line[0]=='@' && line[1]=='S' && line[2]=='Q' && line[3]=='\t');
if(!sq){
myOutstream.write(line);
myOutstream.write('\n');
}
}
}else{
for(byte[] line : list){
myOutstream.write(line);
myOutstream.write('\n');
//myWriter.println(new String(line));
}
}
} catch (IOException e) {
// TODO Auto-generated catch block
e.printStackTrace();
}
}
}else{
if(myWriter!=null){
myWriter.println(SamHeader.header0());
int a=(MINCHROM==-1 ? 1 : MINCHROM);
int b=(MAXCHROM==-1 ? Data.numChroms : MAXCHROM);
for(int chrom=a; chrom<=b; chrom++){
// myWriter.print(SamHeader.header1(chrom, chrom));
SamHeader.printHeader1(chrom, chrom, myWriter);
}
myWriter.println(SamHeader.header2());
}else{
ByteBuilder bb=new ByteBuilder(4096);
SamHeader.header0B(bb);
bb.nl();
int a=(MINCHROM==-1 ? 1 : MINCHROM);
int b=(MAXCHROM==-1 ? Data.numChroms : MAXCHROM);
if(!supressHeaderSequences){
for(int chrom=a; chrom<=b; chrom++){
SamHeader.printHeader1B(chrom, chrom, bb, myOutstream);
}
}
SamHeader.header2B(bb);
bb.nl();
try {
if(bb.length>0){myOutstream.write(bb.array, 0, bb.length);}
} catch (IOException e) {
KillSwitch.exceptionKill(e);
}
}
}
}else if(ff.bread() && !supressHeader){
if(myWriter!=null){
myWriter.println("#"+Read.header());
}else{
try {
myOutstream.write(("#"+Read.header()).getBytes());
} catch (IOException e) {
KillSwitch.exceptionKill(e);
}
}
}
}
assert(bufferSize>=1);
queue=new ArrayBlockingQueue<Job>(bufferSize);
}
/*--------------------------------------------------------------*/
/*---------------- Outer Methods ----------------*/
/*--------------------------------------------------------------*/
@Override
public abstract void run();
/** Uses this thread to transform reads to text, and the ReadStreamWriter thread to write text to disk */
public final synchronized void addListAsText(ArrayList<Read> list){
assert(false) : "TODO";
addList(list, myWriter, myOutstream, false);
}
public final synchronized void poison(){
addJob(new Job(null, null, null, false, true));
}
public final synchronized void addList(ArrayList<Read> list){
addList(list, myWriter, myOutstream, false);
}
public final synchronized void addList(ArrayList<Read> l, PrintWriter w, OutputStream o, boolean c){
boolean poison=(c && w!=null && w==myWriter);
Job j=new Job(l, w, o, c, poison);
addJob(j);
}
public final synchronized void addJob(Job j){
// System.err.println("Got job "+(j.list==null ? "null" : j.list.size()));
boolean success=false;
while(!success){
try {
queue.put(j);
success=true;
} catch (InterruptedException e) {
// TODO Auto-generated catch block
e.printStackTrace();
assert(!queue.contains(j)); //Hopefully it was not added.
}
}
}
/*--------------------------------------------------------------*/
/*---------------- Inner Methods ----------------*/
/*--------------------------------------------------------------*/
protected static final ByteBuilder toQualityB(final byte[] quals, final int len, final int wrap, final ByteBuilder bb){
if(quals==null){return fakeQualityB(30, len, wrap, bb);}
assert(quals.length==len);
bb.ensureExtra(NUMERIC_QUAL ? len*3+1 : len+1);
if(NUMERIC_QUAL){
if(len>0){bb.append((int)quals[0]);}
for(int i=1, w=1; i<len; i++, w++){
if(w>=wrap){
bb.nl();
w=0;
}else{
bb.append(' ');
}
bb.append((int)quals[i]);
}
}else{
final byte b=FASTQ.ASCII_OFFSET_OUT;
for(int i=0; i<len; i++){
bb.append(b+quals[i]);
}
}
return bb;
}
protected static final ByteBuilder fakeQualityB(final int q, final int len, final int wrap, final ByteBuilder bb){
bb.ensureExtra(NUMERIC_QUAL ? len*3+1 : len+1);
if(NUMERIC_QUAL){
int c=(q+FASTQ.ASCII_OFFSET_OUT);
if(len>0){bb.append(q);}
for(int i=1, w=1; i<len; i++, w++){
if(w>=wrap){
bb.nl();
w=0;
}else{
bb.append(' ');
}
bb.append(q);
}
}else{
byte c=(byte)(q+FASTQ.ASCII_OFFSET_OUT);
for(int i=0; i<len; i++){bb.append(c);}
}
return bb;
}
/*--------------------------------------------------------------*/
/*---------------- Getters ----------------*/
/*--------------------------------------------------------------*/
public String fname(){return fname;}
public long readsWritten(){return readsWritten;}
public long basesWritten(){return basesWritten;}
/** Return true if this stream has detected an error */
public final boolean errorState(){return errorState;}
/** Return true if this stream has finished */
public final boolean finishedSuccessfully(){return finishedSuccessfully;}
/*--------------------------------------------------------------*/
/*---------------- Fields ----------------*/
/*--------------------------------------------------------------*/
/** TODO */
protected boolean errorState=false;
protected boolean finishedSuccessfully=false;
public final boolean OUTPUT_SAM;
public final boolean OUTPUT_BAM;
public final boolean OUTPUT_FASTQ;
public final boolean OUTPUT_FASTA;
public final boolean OUTPUT_FASTR;
public final boolean OUTPUT_HEADER;
public final boolean OUTPUT_ATTACHMENT;
public final boolean OUTPUT_ONELINE;
public final boolean OUTPUT_STANDARD_OUT;
public final boolean SITES_ONLY;
public boolean OUTPUT_INTERLEAVED=false;
protected final int FASTA_WRAP;
protected final boolean allowSubprocess;
protected final boolean read1;
protected final String fname;
protected final String qfname;
protected final OutputStream myOutstream;
protected final PrintWriter myWriter;
protected final OutputStream myQOutstream;
protected final PrintWriter myQWriter;
protected final ArrayBlockingQueue<Job> queue;
protected long readsWritten=0;
protected long basesWritten=0;
/*--------------------------------------------------------------*/
/*---------------- Static Fields ----------------*/
/*--------------------------------------------------------------*/
public static int MINCHROM=-1; //For generating sam header
public static int MAXCHROM=-1; //For generating sam header
public static CharSequence HEADER;
public static boolean NUMERIC_QUAL=true;
public static boolean OUTPUT_SAM_SECONDARY_ALIGNMENTS=false;
public static boolean ignorePairAssertions=false;
public static boolean ASSERT_CIGAR=false;
public static boolean NO_HEADER=false;
public static boolean NO_HEADER_SEQUENCES=false;
public static boolean USE_ATTACHED_SAMLINE=false;
/*--------------------------------------------------------------*/
/*---------------- Inner Classes ----------------*/
/*--------------------------------------------------------------*/
protected static class Job{
public Job(ArrayList<Read> list_, PrintWriter writer_, OutputStream outstream_, boolean closeWhenDone_,
boolean shutdownThread_){
list=list_;
writer=writer_;
outstream=outstream_;
close=closeWhenDone_;
poison=shutdownThread_;
}
public Job(ArrayList<Read> list_, PrintWriter writer_){
this(list_, writer_, null, false, false);
}
/*--------------------------------------------------------------*/
public boolean isEmpty(){return list==null || list.isEmpty();}
public final ArrayList<Read> list;
public final PrintWriter writer;
public final OutputStream outstream;
public final boolean close;
public final boolean poison;
}
}
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