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package template;
import java.io.PrintStream;
import java.util.ArrayList;
import java.util.concurrent.atomic.AtomicLong;
import fileIO.ByteFile;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import stream.ConcurrentReadInputStream;
import stream.ConcurrentReadOutputStream;
import stream.FASTQ;
import stream.FastaReadInputStream;
import stream.Read;
import structures.ListNum;
import tracker.ReadStats;
/**
* This class does nothing.
* It serves as a template for generating reads,
* potentially based on some input sequence.
*
* @author Brian Bushnell
* @date June 8, 2019
*
*/
public class A_Sample_Generator {
/*--------------------------------------------------------------*/
/*---------------- Initialization ----------------*/
/*--------------------------------------------------------------*/
/**
* Code entrance from the command line.
* @param args Command line arguments
*/
public static void main(String[] args){
//Start a timer immediately upon code entrance.
Timer t=new Timer();
//Create an instance of this class
A_Sample_Generator x=new A_Sample_Generator(args);
//Run the object
x.process(t);
//Close the print stream if it was redirected
Shared.closeStream(x.outstream);
}
/**
* Constructor.
* @param args Command line arguments
*/
public A_Sample_Generator(String[] args){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, getClass(), false);
args=pp.args;
outstream=pp.outstream;
}
//Set shared static variables prior to parsing
ReadWrite.USE_PIGZ=ReadWrite.USE_UNPIGZ=true;
ReadWrite.setZipThreads(Shared.threads());
FASTQ.TEST_INTERLEAVED=FASTQ.FORCE_INTERLEAVED=false;
{//Parse the arguments
final Parser parser=parse(args);
Parser.processQuality();
maxReads=parser.maxReads;
overwrite=ReadStats.overwrite=parser.overwrite;
append=ReadStats.append=parser.append;
in1=parser.in1;
extin=parser.extin;
out1=parser.out1;
out2=parser.out2;
qfout1=parser.qfout1;
qfout2=parser.qfout2;
extout=parser.extout;
}
validateParams();
doPoundReplacement(); //Replace # with 1 and 2
fixExtensions(); //Add or remove .gz or .bz2 as needed
checkFileExistence(); //Ensure files can be read and written
checkStatics(); //Adjust file-related static fields as needed for this program
//Create output FileFormat objects
ffout1=FileFormat.testOutput(out1, FileFormat.FASTQ, extout, true, overwrite, append, false);
ffout2=FileFormat.testOutput(out2, FileFormat.FASTQ, extout, true, overwrite, append, false);
//Create input FileFormat objects
ffin1=FileFormat.testInput(in1, FileFormat.FASTQ, extin, true, true);
}
/*--------------------------------------------------------------*/
/*---------------- Initialization Helpers ----------------*/
/*--------------------------------------------------------------*/
/** Parse arguments from the command line */
private Parser parse(String[] args){
//Create a parser object
Parser parser=new Parser();
//Set any necessary Parser defaults here
//parser.foo=bar;
//Parse each argument
for(int i=0; i<args.length; i++){
String arg=args[i];
//Break arguments into their constituent parts, in the form of "a=b"
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(b!=null && b.equalsIgnoreCase("null")){b=null;}
if(a.equals("verbose")){
verbose=Parse.parseBoolean(b);
}else if(a.equals("parse_flag_goes_here")){
long fake_variable=Parse.parseKMG(b);
//Set a variable here
}else if(parser.parse(arg, a, b)){//Parse standard flags in the parser
//do nothing
}else{
outstream.println("Unknown parameter "+args[i]);
assert(false) : "Unknown parameter "+args[i];
}
}
return parser;
}
/** Replace # with 1 and 2 in headers */
private void doPoundReplacement(){
//Do output file # replacement
if(out1!=null && out2==null && out1.indexOf('#')>-1){
out2=out1.replace("#", "2");
out1=out1.replace("#", "1");
}
//Ensure there is an input file
assert(false) : "May or may not be required...";
// if(in1==null){throw new RuntimeException("Error - at least one input file is required.");}
//Ensure out2 is not set without out1
if(out1==null && out2!=null){throw new RuntimeException("Error - cannot define out2 without defining out1.");}
}
/** Add or remove .gz or .bz2 as needed */
private void fixExtensions(){
in1=Tools.fixExtension(in1);
}
/** Ensure files can be read and written */
private void checkFileExistence(){
//Ensure output files can be written
if(!Tools.testOutputFiles(overwrite, append, false, out1, out2)){
outstream.println((out1==null)+", "+(out2==null)+", "+out1+", "+out2);
throw new RuntimeException("\n\noverwrite="+overwrite+"; Can't write to output files "+out1+", "+out2+"\n");
}
//Ensure input files can be read
if(!Tools.testInputFiles(false, true, in1)){
throw new RuntimeException("\nCan't read some input files.\n");
}
//Ensure that no file was specified multiple times
if(!Tools.testForDuplicateFiles(true, in1, out1, out2)){
throw new RuntimeException("\nSome file names were specified multiple times.\n");
}
}
/** Adjust file-related static fields as needed for this program */
private static void checkStatics(){
//Adjust the number of threads for input file reading
if(!ByteFile.FORCE_MODE_BF1 && !ByteFile.FORCE_MODE_BF2 && Shared.threads()>2){
ByteFile.FORCE_MODE_BF2=true;
}
assert(FastaReadInputStream.settingsOK());
}
/** Ensure parameter ranges are within bounds and required parameters are set */
private boolean validateParams(){
// assert(minfoo>0 && minfoo<=maxfoo) : minfoo+", "+maxfoo;
assert(false) : "TODO";
return true;
}
/*--------------------------------------------------------------*/
/*---------------- Outer Methods ----------------*/
/*--------------------------------------------------------------*/
/** Create read streams and process all data */
void process(Timer t){
//Turn off read validation in the input threads to increase speed
final boolean vic=Read.VALIDATE_IN_CONSTRUCTOR;
Read.VALIDATE_IN_CONSTRUCTOR=true;
//Create a read input stream
final ConcurrentReadInputStream cris=makeCris();
//Optionally create a read output stream
assert(false) : "TODO: Determine whether output should be paired.";
final ConcurrentReadOutputStream ros=makeCros(false);
//Reset counters
readsProcessed=readsOut=0;
basesProcessed=basesOut=0;
data=loadData(cris);
//Process the reads in separate threads
spawnThreads(cris, ros);
if(verbose){outstream.println("Finished; closing streams.");}
//Write anything that was accumulated by ReadStats
errorState|=ReadStats.writeAll();
//Close the read streams
errorState|=ReadWrite.closeStreams(cris, ros);
//Reset read validation
Read.VALIDATE_IN_CONSTRUCTOR=vic;
//Report timing and results
t.stop();
outstream.println(Tools.timeReadsBasesProcessed(t, readsProcessed, basesProcessed, 8));
outstream.println(Tools.readsBasesOut(readsProcessed, basesProcessed, readsOut, basesOut, 8, false));
//Throw an exception of there was an error in a thread
if(errorState){
throw new RuntimeException(getClass().getName()+" terminated in an error state; the output may be corrupt.");
}
}
/** Create a Read Input Stream */
private ConcurrentReadInputStream makeCris(){
ConcurrentReadInputStream cris=ConcurrentReadInputStream.getReadInputStream(maxReads, true, ffin1, null);
cris.start(); //Start the stream
if(verbose){outstream.println("Started cris");}
return cris;
}
/** Create a Read Output Stream */
private ConcurrentReadOutputStream makeCros(boolean pairedInput){
if(ffout1==null){return null;}
//Set output buffer size
final int buff=4;
//Notify user of output mode
if(pairedInput && out2==null && (in1!=null && !ffin1.samOrBam() && !ffout1.samOrBam())){
outstream.println("Writing interleaved.");
}
final ConcurrentReadOutputStream ros=ConcurrentReadOutputStream.getStream(
ffout1, ffout2, qfout1, qfout2, buff, null, false);
ros.start(); //Start the stream
return ros;
}
/** Spawn process threads */
private void spawnThreads(final ConcurrentReadInputStream cris, final ConcurrentReadOutputStream ros){
//Do anything necessary prior to processing
//Determine how many threads may be used
final int threads=Shared.threads();
//Fill a list with ProcessThreads
ArrayList<ProcessThread> alpt=new ArrayList<ProcessThread>(threads);
for(int i=0; i<threads; i++){
alpt.add(new ProcessThread(ros, i, maxReads, nextReadID));
}
//Start the threads
for(ProcessThread pt : alpt){
pt.start();
}
//Wait for threads to finish
waitForThreads(alpt);
//Do anything necessary after processing
}
/** Wait until all worker threads are finished, then return */
private void waitForThreads(ArrayList<ProcessThread> alpt){
//Wait for completion of all threads
boolean success=true;
for(ProcessThread pt : alpt){
//Wait until this thread has terminated
while(pt.getState()!=Thread.State.TERMINATED){
try {
//Attempt a join operation
pt.join();
} catch (InterruptedException e) {
//Potentially handle this, if it is expected to occur
e.printStackTrace();
}
}
//Accumulate per-thread statistics
readsOut+=pt.readsOutT;
basesOut+=pt.basesOutT;
success&=pt.success;
}
//Track whether any threads failed
if(!success){errorState=true;}
}
/*--------------------------------------------------------------*/
/*---------------- Inner Methods ----------------*/
/*--------------------------------------------------------------*/
private ArrayList<Read> loadData(ConcurrentReadInputStream cris){
ArrayList<Read> input=new ArrayList<Read>();
//Grab the first ListNum of reads
ListNum<Read> ln=cris.nextList();
//As long as there is a nonempty read list...
while(ln!=null && ln.size()>0){
// if(verbose){outstream.println("Fetched "+reads.size()+" reads.");} //Disabled due to non-static access
for(Read r : ln){
//Optional filter criteria
if(true){
input.add(r);
}
//Increment counters
readsProcessed+=r.pairCount();
basesProcessed+=r.pairLength();
}
//Notify the input stream that the list was used
cris.returnList(ln);
// if(verbose){outstream.println("Returned a list.");} //Disabled due to non-static access
//Fetch a new list
ln=cris.nextList();
}
//Notify the input stream that the final list was used
if(ln!=null){
cris.returnList(ln.id, ln.list==null || ln.list.isEmpty());
}
return input;
}
/*--------------------------------------------------------------*/
/*---------------- Inner Classes ----------------*/
/*--------------------------------------------------------------*/
/** This class is static to prevent accidental writing to shared variables.
* It is safe to remove the static modifier. */
private static class ProcessThread extends Thread {
//Constructor
ProcessThread(final ConcurrentReadOutputStream ros_, final int tid_,
final long maxReads_, final AtomicLong nextReadID_){
ros=ros_;
tid=tid_;
maxReads=maxReads_;
atomicReadID=nextReadID_;
}
//Called by start()
@Override
public void run(){
//Do anything necessary prior to processing
//Process the reads
processInner();
//Do anything necessary after processing
//Indicate successful exit status
success=true;
}
/** Iterate through the reads */
void processInner(){
//As long as there is a nonempty read list...
for(long generated=atomicReadID.getAndAdd(readsPerList); generated<maxReads;
generated=atomicReadID.getAndAdd(readsPerList)){
// if(verbose){outstream.println("Fetched "+reads.size()+" reads.");} //Disabled due to non-static access
long toGenerate=Tools.min(readsPerList, maxReads-generated);
ArrayList<Read> reads=generateList((int)toGenerate, generated);
//Output reads to the output stream
if(ros!=null){ros.add(reads, 0);}
}
}
/** Generate the next list of reads */
private ArrayList<Read> generateList(int toGenerate, long nextID){
//Grab the actual read list from the ListNum
final ArrayList<Read> reads=new ArrayList<Read>(toGenerate);
//Loop through each read in the list
for(int i=0; i<toGenerate; i++, nextID++){
Read r=generateRead(nextID);
readsOutT+=r.pairCount();
basesOutT+=r.length();
reads.add(r);
}
return reads;
}
/**
* Generate a single read.
*/
private Read generateRead(final long nextID){
// Read r=new Read(null, null, nextID);
throw new RuntimeException("TODO: Implement this method."); //TODO
}
/** Number of reads retained by this thread */
protected long readsOutT=0;
/** Number of bases retained by this thread */
protected long basesOutT=0;
/** True only if this thread has completed successfully */
boolean success=false;
private final AtomicLong atomicReadID;
private final long maxReads;
private final int readsPerList=Shared.bufferLen();
/** Shared output stream */
private final ConcurrentReadOutputStream ros;
/** Thread ID */
final int tid;
}
/*--------------------------------------------------------------*/
/*---------------- Fields ----------------*/
/*--------------------------------------------------------------*/
/** Primary input file path */
private String in1=null;
/** Primary output file path */
private String out1=null;
/** Secondary output file path */
private String out2=null;
private String qfout1=null;
private String qfout2=null;
/** Override input file extension */
private String extin=null;
/** Override output file extension */
private String extout=null;
/*--------------------------------------------------------------*/
/** Number of reads processed */
protected long readsProcessed=0;
/** Number of bases processed */
protected long basesProcessed=0;
/** Number of reads retained */
protected long readsOut=0;
/** Number of bases retained */
protected long basesOut=0;
/** Quit after generating this many OUTPUT reads */
private long maxReads=-1;
/** Input read storage, if needed */
private ArrayList<Read> data=new ArrayList<Read>();
private AtomicLong nextReadID=new AtomicLong(0);
/*--------------------------------------------------------------*/
/*---------------- Final Fields ----------------*/
/*--------------------------------------------------------------*/
/** Primary input file */
private final FileFormat ffin1;
/** Primary output file */
private final FileFormat ffout1;
/** Secondary output file */
private final FileFormat ffout2;
/*--------------------------------------------------------------*/
/*---------------- Common Fields ----------------*/
/*--------------------------------------------------------------*/
/** Print status messages to this output stream */
private PrintStream outstream=System.err;
/** Print verbose messages */
public static boolean verbose=false;
/** True if an error was encountered */
public boolean errorState=false;
/** Overwrite existing output files */
private boolean overwrite=true;
/** Append to existing output files */
private boolean append=false;
}
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