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package var2;
import java.io.PrintStream;
import java.util.ArrayList;
import java.util.concurrent.ArrayBlockingQueue;
import fileIO.ByteFile;
import fileIO.ByteFile1;
import fileIO.ByteFile2;
import fileIO.ByteStreamWriter;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import stream.ConcurrentGenericReadInputStream;
import stream.FastaReadInputStream;
import structures.ByteBuilder;
import structures.ListNum;
import structures.StringPair;
import var2.CallVariants2.Sample;
/**
* @author Brian Bushnell
* @date December 18, 2016
*
*/
public class MergeSamples {
public static void main(String[] args){
Timer t=new Timer();
MergeSamples x=new MergeSamples(args);
//x.process(t);
//Close the print stream if it was redirected
Shared.closeStream(x.outstream);
}
public MergeSamples(){
threads=Shared.threads();
inq=new ArrayBlockingQueue<ListNum<VCFLine[]>>(threads+1);
}
public MergeSamples(String[] args){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, getClass(), false);
args=pp.args;
outstream=pp.outstream;
}
ReadWrite.USE_PIGZ=ReadWrite.USE_UNPIGZ=true;
ReadWrite.setZipThreads(Shared.threads());
Parser parser=new Parser();
for(int i=0; i<args.length; i++){
String arg=args[i];
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(parser.parse(arg, a, b)){
//do nothing
}else if(a.equals("invalid")){
outInvalid=b;
}else if(a.equals("lines")){
maxLines=Long.parseLong(b);
if(maxLines<0){maxLines=Long.MAX_VALUE;}
}else if(a.equals("verbose")){
verbose=Parse.parseBoolean(b);
ByteFile1.verbose=verbose;
ByteFile2.verbose=verbose;
stream.FastaReadInputStream.verbose=verbose;
ConcurrentGenericReadInputStream.verbose=verbose;
stream.FastqReadInputStream.verbose=verbose;
ReadWrite.verbose=verbose;
}else{
outstream.println("Unknown parameter "+args[i]);
assert(false) : "Unknown parameter "+args[i];
// throw new RuntimeException("Unknown parameter "+args[i]);
}
}
{//Process parser fields
overwrite=parser.overwrite;
append=parser.append;
in1=parser.in1;
out1=parser.out1;
}
assert(FastaReadInputStream.settingsOK());
if(in1==null){throw new RuntimeException("Error - at least one input file is required.");}
if(!ByteFile.FORCE_MODE_BF2){
ByteFile.FORCE_MODE_BF2=false;
ByteFile.FORCE_MODE_BF1=true;
}
if(out1!=null && out1.equalsIgnoreCase("null")){out1=null;}
if(!Tools.testOutputFiles(overwrite, append, false, out1)){
outstream.println((out1==null)+", "+out1);
throw new RuntimeException("\n\noverwrite="+overwrite+"; Can't write to output files "+out1+"\n");
}
threads=Shared.threads();
inq=new ArrayBlockingQueue<ListNum<VCFLine[]>>(threads+1);
}
/*--------------------------------------------------------------*/
public void mergeSamples(ArrayList<Sample> list, ScafMap scafMap, String outVcf, String scoreHistFile){
map=scafMap;
ArrayList<StringPair> vcfList=new ArrayList<StringPair>(list.size());
for(Sample s : list){vcfList.add(new StringPair(s.name, s.vcfName));}
mergeFiles(vcfList, outVcf, scoreHistFile);
}
public void mergeFiles(ArrayList<StringPair> list, String outVcf, String scoreHistFile){
System.err.println("Merging "+list);
final int ways=list.size();
ByteFile[] bfa=new ByteFile[ways];
final boolean allowSubprocess=(ways<=4);
for(int i=0; i<ways; i++){
StringPair pair=list.get(i);
FileFormat ff=FileFormat.testInput(pair.b, FileFormat.VCF, null, allowSubprocess, false);
bfa[i]=ByteFile.makeByteFile(ff);
}
// System.err.println("Made byte files.");
// assert(false) : outVcf;
// System.err.println("Started writer.");
mergeMT(outVcf, bfa);
if(scoreHistFile!=null){
CallVariants.writeScoreHist(scoreHistFile, scoreArray);
}
// System.err.println("Closed stream.");
}
private void mergeST(String outVcf, ByteFile[] bfa){
ByteStreamWriter bswVcf=null;
if(outVcf!=null){
bswVcf=new ByteStreamWriter(outVcf, true, false, true, FileFormat.VCF);
bswVcf.start();
}
ByteBuilder bb=new ByteBuilder(34000);
VCFLine[] row=processRow(bfa, bb);
while(row!=null){
// System.err.println("Processed a line.");
if(row[0]!=null){
VCFLine merged=merge(row);
merged.toText(bb);
bb.nl();
if(bb.length>32000){
if(bswVcf!=null){bswVcf.print(bb);}
bb=new ByteBuilder(34000);
}
}
row=processRow(bfa, bb);
}
// System.err.println("Finished processing.");
if(bswVcf!=null){
if(bb.length>0){bswVcf.print(bb);}
bswVcf.poisonAndWait();
}
}
private void mergeMT(String outVcf, ByteFile[] bfa){
ByteStreamWriter bswVcf=null;
if(outVcf!=null){
FileFormat ff=FileFormat.testOutput(outVcf, FileFormat.VCF, null, true, true, append, true);
bswVcf=new ByteStreamWriter(ff);
bswVcf.start();
}
ArrayList<MergeThread> alpt=spawnThreads(bswVcf);
long nextID=0;
ByteBuilder header=new ByteBuilder(34000);
VCFLine[] row=processRow(bfa, header);
while(row!=null && row[0]==null){//Header
row=processRow(bfa, header);
}
if(bswVcf!=null){
bswVcf.add(header, nextID);
nextID++;
}
ListNum<VCFLine[]> list=new ListNum<VCFLine[]>(new ArrayList<VCFLine[]>(200), nextID);
while(row!=null){
if(row[0]!=null){
list.add(row);
if(list.size()>=200){
putList(list);
nextID++;
list=new ListNum<VCFLine[]>(new ArrayList<VCFLine[]>(200), nextID);
}
}
row=processRow(bfa, header);
}
if(list.size()>0){
putList(list);
nextID++;
}
putList(POISON_LIST);
waitForFinish(alpt);
if(bswVcf!=null){bswVcf.poisonAndWait();}
}
VCFLine[] processRow(ByteFile[] bfa, ByteBuilder bb){
byte[][] lines=new byte[bfa.length][];
for(int i=0; i<bfa.length; i++){
byte[] line=bfa[i].nextLine();
if(line==null){return null;}
lines[i]=line;
}
VCFLine[] row=new VCFLine[bfa.length];
if(lines[0][0]=='#'){
processHeader(lines, bb);
return row;
}
for(int i=0; i<lines.length; i++){
byte[] line=lines[i];
row[i]=new VCFLine(line);
if(i>0){assert(row[i].pos==row[0].pos) : "\n"+row[0]+"\n"+row[i];}
}
return row;
}
void processHeader(byte[][] lines, ByteBuilder bb){
String[][] matrix=new String[lines.length][];
for(int i=0; i<lines.length; i++){
matrix[i]=new String(lines[i]).split("=");
}
if(matrix[0][0].equals("##ploidy")){
ploidy=Integer.parseInt(matrix[0][1]);
bb.append("##ploidy="+ploidy+"\n");
}else if(matrix[0][0].equals("##reads")){
for(String[] split : matrix){
reads+=Long.parseLong(split[1]);
}
bb.append("##reads="+reads+"\n");
}else if(matrix[0][0].equals("##pairedReads")){
for(String[] split : matrix){
pairedReads+=Long.parseLong(split[1]);
}
bb.append("##pairedReads="+pairedReads+"\n");
}else if(matrix[0][0].equals("##properlyPairedReads")){
for(String[] split : matrix){
properlyPairedReads+=Long.parseLong(split[1]);
}
properPairRate=properlyPairedReads*1.0/(Tools.max(1, reads));
bb.append("##properlyPairedReads="+properlyPairedReads+"\n");
bb.append("##properPairRate="+Tools.format("%.4f\n", properPairRate));
}else if(matrix[0][0].equals("##properPairRate")){
//do nothing
}else if(matrix[0][0].equals("##totalQualityAvg")){
totalQualityAvg=0;
for(String[] split : matrix){
totalQualityAvg+=Float.parseFloat(split[1]);
}
totalQualityAvg/=lines.length;
bb.append("##totalQualityAvg="+Tools.format("%.4f\n", totalQualityAvg));
}else if(matrix[0][0].equals("##mapqAvg")){
mapqAvg=0;
for(String[] split : matrix){
mapqAvg+=Float.parseFloat(split[1]);
}
mapqAvg/=lines.length;
bb.append("##mapqAvg="+Tools.format("%.2f\n", mapqAvg));
}else if(matrix[0][0].equals("##readLengthAvg")){
readLengthAvg=0;
for(String[] split : matrix){
readLengthAvg+=Float.parseFloat(split[1]);
}
readLengthAvg/=lines.length;
bb.append("##readLengthAvg="+Tools.format("%.2f\n", readLengthAvg));
}else if(matrix[0][0].startsWith("#CHROM\tPOS\t")){
bb.append(lines[0]);
for(int i=1; i<lines.length; i++){
String[] split=new String(lines[i]).split("\t");
bb.tab().append(split[split.length-1]);
}
bb.nl();
}else{
bb.append(lines[0]);
bb.nl();
}
}
VCFLine merge(VCFLine[] row){
// System.err.println(row.length);
// System.err.println(row[0]);
Var sum=null;
VCFLine best=null;
for(VCFLine line : row){
if(best==null || line.qual>best.qual){best=line;}
Var v=line.toVar();
assert(v!=null);
if(sum==null){sum=v;}
else{
sum.add(v);
sum.addCoverage(v);
}
}
assert(best!=null);
assert(sum!=null) : row.length+", "+row[0];
//ByteBuilder bb, double properPairRate, double totalQualityAvg, double mapqAvg, int ploidy, ScafMap map, VarFilter filter, boolean trimWhitespace
ByteBuilder bb=sum.toVCF(new ByteBuilder(), properPairRate, totalQualityAvg, mapqAvg, readLengthAvg, ploidy, map, filter, trimWhitespace);
VCFLine merged=new VCFLine(bb.toBytes());
merged.samples.clear();
for(VCFLine line : row){
merged.samples.addAll(line.samples);
}
if(merged.qual<best.qual){
merged.qual=best.qual;
merged.filter=best.filter;
}
scoreArray[(int)merged.qual]++;
return merged;
}
/*--------------------------------------------------------------*/
/*--------------------------------------------------------------*/
final ListNum<VCFLine[]> takeList(){
ListNum<VCFLine[]> list=null;
while(list==null){
try {
list=inq.take();
} catch (InterruptedException e) {
// TODO Auto-generated catch block
e.printStackTrace();
}
}
return list;
}
final void putList(ListNum<VCFLine[]> list){
while(list!=null){
try {
inq.put(list);
list=null;
} catch (InterruptedException e) {
// TODO Auto-generated catch block
e.printStackTrace();
}
}
}
/** Spawn process threads */
private ArrayList<MergeThread> spawnThreads(ByteStreamWriter bsw){
//Do anything necessary prior to processing
//Fill a list with MergeThreads
ArrayList<MergeThread> alpt=new ArrayList<MergeThread>(threads);
for(int i=0; i<threads; i++){
alpt.add(new MergeThread(bsw));
}
if(verbose){outstream.println("Spawned threads.");}
//Start the threads
for(MergeThread pt : alpt){
pt.start();
}
if(verbose){outstream.println("Started threads.");}
//Do anything necessary after processing
return alpt;
}
private void waitForFinish(ArrayList<MergeThread> alpt){
//Wait for completion of all threads
boolean allSuccess=true;
for(MergeThread pt : alpt){
while(pt.getState()!=Thread.State.TERMINATED){
try {
//Attempt a join operation
pt.join();
} catch (InterruptedException e) {
//Potentially handle this, if it is expected to occur
e.printStackTrace();
}
}
}
}
private class MergeThread extends Thread {
MergeThread(ByteStreamWriter bsw_){
bsw=bsw_;
}
@Override
public void run(){
ListNum<VCFLine[]> list=takeList();
while(list!=null && list!=POISON_LIST){
processList(list);
list=takeList();
}
putList(POISON_LIST);
}
private void processList(ListNum<VCFLine[]> list){
ByteBuilder bb=new ByteBuilder(4096);
for(VCFLine[] row : list){
mergeRow(row, bb);
}
if(bsw!=null){bsw.add(bb, list.id);}
}
private void mergeRow(VCFLine[] row, ByteBuilder bb){
if(row[0]!=null){
VCFLine merged=merge(row);
merged.toText(bb);
bb.nl();
}
}
private final ByteStreamWriter bsw;
}
/*--------------------------------------------------------------*/
final ListNum<VCFLine[]> POISON_LIST=new ListNum<VCFLine[]>(null, -1);
private final ArrayBlockingQueue<ListNum<VCFLine[]>> inq;
private final int threads;
/*--------------------------------------------------------------*/
long readsSum;
long pairsSum;
int ploidy=1;
double properPairRate;
double totalQualityAvg;
double mapqAvg;
double readLengthAvg;
long reads;
long pairedReads;
long properlyPairedReads;
VarFilter filter;
ScafMap map;
boolean trimWhitespace=true;
private String in1=null;
private String out1=null;
private String outInvalid=null;
long[] scoreArray=new long[200];
/*--------------------------------------------------------------*/
private long linesProcessed=0;
private long linesValid=0;
private long bytesProcessed=0;
private long maxLines=Long.MAX_VALUE;
/*--------------------------------------------------------------*/
private PrintStream outstream=System.err;
public static boolean verbose=false;
public boolean errorState=false;
private boolean overwrite=true;
private boolean append=false;
}
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