File: VCFFile.java

package info (click to toggle)
bbmap 39.20%2Bdfsg-3
  • links: PTS, VCS
  • area: main
  • in suites: forky, sid
  • size: 26,024 kB
  • sloc: java: 312,743; sh: 18,099; python: 5,247; ansic: 2,074; perl: 96; makefile: 39; xml: 38
file content (258 lines) | stat: -rwxr-xr-x 6,728 bytes parent folder | download | duplicates (2)
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
251
252
253
254
255
256
257
258
package var2;

import java.io.PrintStream;
import java.util.ArrayList;
import java.util.LinkedHashMap;
import java.util.Map.Entry;

import fileIO.ByteFile;
import fileIO.ByteFile1;
import fileIO.ByteFile2;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import stream.ConcurrentGenericReadInputStream;
import stream.FastaReadInputStream;

/**
 * @author Brian Bushnell
 * @date January 14, 2017
 *
 */
public class VCFFile {
	
	public static void main(String[] args){
		
		{//Preparse block for help, config files, and outstream
			PreParser pp=new PreParser(args, new Object() { }.getClass().getEnclosingClass(), false);
			args=pp.args;
			outstream=pp.outstream;
		}
		
		ReadWrite.USE_PIGZ=ReadWrite.USE_UNPIGZ=true;
		ReadWrite.setZipThreads(Shared.threads());
		VCFLine.AUTOCACHE=true;
		
		Timer t=new Timer();
		String in=null;
		long maxLines=Long.MAX_VALUE;
		
		Parser parser=new Parser();
		for(int i=0; i<args.length; i++){
			String arg=args[i];
			String[] split=arg.split("=");
			String a=split[0].toLowerCase();
			String b=split.length>1 ? split[1] : null;

			if(parser.parse(arg, a, b)){
				//do nothing
			}else if(a.equals("lines")){
				maxLines=Long.parseLong(b);
				if(maxLines<0){maxLines=Long.MAX_VALUE;}
			}else if(a.equals("verbose")){
				verbose=Parse.parseBoolean(b);
				ByteFile1.verbose=verbose;
				ByteFile2.verbose=verbose;
				stream.FastaReadInputStream.verbose=verbose;
				ConcurrentGenericReadInputStream.verbose=verbose;
				stream.FastqReadInputStream.verbose=verbose;
				ReadWrite.verbose=verbose;
			}else{
				outstream.println("Unknown parameter "+args[i]);
				assert(false) : "Unknown parameter "+args[i];
				//				throw new RuntimeException("Unknown parameter "+args[i]);
			}
		}
		
		{//Process parser fields
			in=parser.in1;
		}
		
		assert(FastaReadInputStream.settingsOK());
		
		if(in==null){throw new RuntimeException("Error - at least one input file is required.");}
		
		if(!ByteFile.FORCE_MODE_BF2){
			ByteFile.FORCE_MODE_BF2=false;
			ByteFile.FORCE_MODE_BF1=true;
		}
		
		VCFFile vf=new VCFFile(in);
		t.stop();
		vf.printTime(t);
		
		//Close the print stream if it was redirected
		Shared.closeStream(outstream);
	}
	
	public VCFFile(String s){
		in1=s;
		ffin1=FileFormat.testInput(in1, FileFormat.TEXT, null, true, false);
		load();
	}
	
	public VCFFile(FileFormat ff){
		in1=ff.name();
		ffin1=ff;
		load();
	}
	
	void load(){
		
		ByteFile bf=ByteFile.makeByteFile(ffin1);
		
		byte[] line=bf.nextLine();
		
		while(line!=null && (line.length==0 || line[0]=='#')){
			if(line.length>0){
				if(maxLines>0 && linesProcessed>=maxLines){break;}
				linesProcessed++;
				bytesProcessed+=line.length;
				header.add(line);
				if(Tools.startsWith(line, CHROM_POS)){
					String[] split=new String(line).split("\t");
					for(int i=9; i<split.length; i++){
						sampleNames.add(split[i]);
					}
				}
			}
			line=bf.nextLine();
		}
		if(ScafMap.defaultScafMap()==null){ScafMap.setDefaultScafMap(toScafMap(null), in1);}
		assert(ScafMap.defaultScafMap().size()>0) : ScafMap.defaultScafMap()+"\n"+headerToString();
		while(line!=null){
			if(line.length>0){
				if(maxLines>0 && linesProcessed>=maxLines){break;}
				linesProcessed++;
				bytesProcessed+=line.length;
				
				final boolean isHeader=(line[0]=='#');
				
				if(isHeader){
					header.add(line);
					if(Tools.startsWith(line, CHROM_POS)){
						String[] split=new String(line).split("\t");
						for(int i=9; i<split.length; i++){
							sampleNames.add(split[i]);
						}
					}
				}else{
					VCFLine vline=new VCFLine(line);
					map.put(vline, vline);
				}
			}
			line=bf.nextLine();
		}
		
		errorState|=bf.close();
	}
	
//	static ArrayList<byte[]> loadHeaderOnly(String fname){
//		ByteFile bf=ByteFile.makeByteFile(fname, true);
//		ArrayList<byte[]> header=new ArrayList<byte[]>();
//		byte[] line=bf.nextLine();
//		while(line!=null && (line.length==0 || line[0]=='#')){
//			if(line.length>0){
//				header.add(line);
//			}
//			line=bf.nextLine();
//		}
//	}
	
	void printTime(Timer t){
		outstream.println(Tools.timeLinesBytesProcessed(t, linesProcessed, bytesProcessed, 8));
		
		outstream.println();
		outstream.println("Header Lines:      \t"+header.size());
		outstream.println("Variant Lines:     \t"+map.size());
	}
	
	public ArrayList<VCFLine> lines(boolean simplify){
		ArrayList<VCFLine> lines=new ArrayList<VCFLine>(map.size());
		for(Entry<VCFLine, VCFLine> e : map.entrySet()){
			VCFLine line=e.getValue();
			if(simplify && (line.isMulti() || line.isComplex())){
				ArrayList<VCFLine> list=line.split(true, true, false);
				lines.addAll(list);
			}else{
				lines.add(line);
			}
		}
		return lines;
	}
	
//	public static ScafMap toScafMap(String vcf){
//		assert(vcf!=null);
//		if(vcf==null){return null;}
//		if(sm==null){sm=new ScafMap();}
//		for(byte[] line : header){
//			if(Tools.startsWith(line, "##contig=<ID=")){
//				sm.addFromVcf(line);
//			}
//		}
//		return sm;
//	}
	
	public ScafMap toScafMap(ScafMap sm){
		if(sm==null){sm=new ScafMap();}
		for(byte[] line : header){
			if(Tools.startsWith(line, "##contig=<ID=")){
				sm.addFromVcf(line);
			}
		}
		return sm;
	}
	
	public String headerToString(){
		StringBuilder sb=new StringBuilder();
		for(byte[] line : header){
			for(byte b : line){
				sb.append((char)b);
			}
			sb.append('\n');
		}
		return sb.toString();
	}
	
	/*--------------------------------------------------------------*/
	
	public long linesProcessed() {
		return linesProcessed;
	}
	
	public long bytesProcessed() {
		return bytesProcessed;
	}
	
	/*--------------------------------------------------------------*/
	
	private long linesProcessed=0;
	private long bytesProcessed=0;
	
	private long maxLines=Long.MAX_VALUE;
	
	public ArrayList<byte[]> header=new ArrayList<byte[]>();
	public ArrayList<String> sampleNames=new ArrayList<String>();
	public LinkedHashMap<VCFLine, VCFLine> map=new LinkedHashMap<VCFLine, VCFLine>();
	
	/*--------------------------------------------------------------*/
	
	private String in1=null;
	
	private final FileFormat ffin1;
	
	
	/*--------------------------------------------------------------*/
	
	public static final String CHROM_POS="#CHROM\tPOS\t";
	
	private static PrintStream outstream=System.err;
	public static boolean verbose=false;
	public boolean errorState=false;
	
}