1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300 301 302 303 304 305 306 307 308 309 310 311 312 313 314 315 316 317 318 319 320 321 322 323 324 325 326
|
package var2;
import java.io.PrintStream;
import java.util.ArrayList;
import java.util.concurrent.ArrayBlockingQueue;
import fileIO.ByteFile;
import fileIO.FileFormat;
import shared.Shared;
import shared.Tools;
import structures.ListNum;
public class VcfLoader {
public VcfLoader(String fname_, ScafMap scafMap_, boolean vcfMode_){
fname=fname_;
scafMap=scafMap_;
varMap=new VarMap(scafMap);
threads=Tools.max(1, Shared.threads());
inq=new ArrayBlockingQueue<ListNum<byte[]>>(threads);
vcfMode=vcfMode_;
ffin=FileFormat.testInput(fname, FileFormat.TXT, null, true, false);
}
public static VarMap loadFile(FileFormat ff, ScafMap scafMap, boolean loadCoverage, boolean extendedInfo){
final VarMap varMap;
if(ff.var()){
varMap=VcfLoader.loadVarFile(ff.name(), scafMap);
}else{
varMap=VcfLoader.loadVcfFile(ff.name(), scafMap, loadCoverage, extendedInfo);
}
return varMap;
}
public static VarMap loadVarFile(String fname, ScafMap scafMap){
VcfLoader loader=new VcfLoader(fname, scafMap, false);
ArrayList<ProcessThread> alpt=loader.spawnThreads(false, false);
loader.waitForFinish(alpt);
return loader.varMap;
}
public static VarMap loadVcfFile(String fname, ScafMap scafMap, boolean loadCoverage, boolean extendedInfo){
VcfLoader loader=new VcfLoader(fname, scafMap, true);
ArrayList<ProcessThread> alpt=loader.spawnThreads(loadCoverage, extendedInfo);
loader.waitForFinish(alpt);
return loader.varMap;
}
/** Spawn process threads */
private ArrayList<ProcessThread> spawnThreads(boolean loadCoverage, boolean extendedInfo){
//Do anything necessary prior to processing
//Determine how many threads may be used
final int threads=this.threads+1;//1 extra for thread 0, which is different than the others.
assert(threads>=2);
//Fill a list with ProcessThreads
ArrayList<ProcessThread> alpt=new ArrayList<ProcessThread>(threads);
for(int i=0; i<threads; i++){
alpt.add(new ProcessThread(i, alpt, loadCoverage, extendedInfo));
}
if(verbose){outstream.println("Spawned threads.");}
//Start the threads
for(ProcessThread pt : alpt){
pt.start();
}
if(verbose){outstream.println("Started threads.");}
//Do anything necessary after processing
return alpt;
}
private void waitForFinish(ArrayList<ProcessThread> alpt){
//Wait for completion of all threads
boolean allSuccess=true;
for(ProcessThread pt : alpt){
if(verbose){outstream.println("Waiting for thread "+pt.tid);}
while(pt.getState()!=Thread.State.TERMINATED){
try {
//Attempt a join operation
pt.join();
} catch (InterruptedException e) {
//Potentially handle this, if it is expected to occur
e.printStackTrace();
}
}
allSuccess&=pt.success;
}
//Track whether any threads failed
if(!allSuccess){errorState=true;}
}
private Var loadVarLine(byte[] line){
if(line==null || line.length<1){return null;}
if(line[0]!='#'){
return new Var(line, (byte)'\t');
}else{
String[] split=new String(line).split("\t");
String a=split[0], b=(split.length>1 ? split[1] : null);
assert(split.length>1) : new String(line);
if(a.equalsIgnoreCase("#ploidy")){
synchronized(varMap){varMap.ploidy=Integer.parseInt(b);}
}else if(a.equalsIgnoreCase("#pairingRate")){
synchronized(varMap){varMap.properPairRate=Double.parseDouble(b);}
}else if(a.equalsIgnoreCase("#totalQualityAvg")){
synchronized(varMap){varMap.totalQualityAvg=Double.parseDouble(b);}
}else if(a.equalsIgnoreCase("#mapqAvg")){
synchronized(varMap){varMap.totalMapqAvg=Double.parseDouble(b);}
}else if(a.equalsIgnoreCase("#readLengthAvg")){
synchronized(varMap){varMap.readLengthAvg=Double.parseDouble(b);}
}
synchronized(header){header.add(line);}
return null;
}
}
private Var loadVcfLine(byte[] line, boolean loadCoverage, boolean loadExtended){
if(line==null || line.length<1){return null;}
if(line[0]!='#'){
try {
return Var.fromVCF(line, scafMap, loadCoverage, loadExtended);
} catch (Exception e) {
System.err.println("Unable to parse VCF line: '"+new String(line)+"'");
return null;
}
}else{
String[] split=new String(line).split("=");
if(split.length==2){
String a=split[0], b=split[1];
if(a.equalsIgnoreCase("##ploidy")){
synchronized(varMap){varMap.ploidy=Integer.parseInt(b);}
}else if(a.equalsIgnoreCase("##properPairRate")){
synchronized(varMap){varMap.properPairRate=Double.parseDouble(b);}
}else if(a.equalsIgnoreCase("##totalQualityAvg")){
synchronized(varMap){varMap.totalQualityAvg=Double.parseDouble(b);}
}else if(a.equalsIgnoreCase("##mapqAvg")){
synchronized(varMap){varMap.totalMapqAvg=Double.parseDouble(b);}
}else if(a.equalsIgnoreCase("##readLengthAvg")){
synchronized(varMap){varMap.readLengthAvg=Double.parseDouble(b);}
}
}
synchronized(header){header.add(line);}
return null;
}
}
private class ProcessThread extends Thread{
ProcessThread(int tid_, ArrayList<ProcessThread> alpt_, boolean loadCoverage_, boolean extendedInfo_){
tid=tid_;
alpt=(tid==0 ? alpt_ : null);
loadCoverage=loadCoverage_;
extendedInfo=extendedInfo_;
}
@Override
public void run(){
//Do anything necessary prior to processing
//Process the reads
if(tid==0){
processBytes();
}else{
makeVars();
}
//Indicate successful exit status
success=true;
}
void processBytes(){
processBytes0();
success=true;
}
public final void processBytes0(){
if(verbose){outstream.println("tid "+tid+" started processBytes.");}
// ByteFile.FORCE_MODE_BF1=true;
ByteFile.FORCE_MODE_BF2=true;
ByteFile bf=ByteFile.makeByteFile(ffin);
long number=0;
ArrayList<byte[]> list=new ArrayList<byte[]>(LIST_SIZE);
for(byte[] line=bf.nextLine(); line!=null; line=bf.nextLine()){
assert(line!=null);
// outstream.println("a");
if(line[0]=='#'){
if(vcfMode){loadVcfLine(line, false, false);}
else{loadVarLine(line);}
}else{
list.add(line);
if(list.size()>=LIST_SIZE){
putBytes(new ListNum<byte[]>(list, number));
number++;
list=new ArrayList<byte[]>(LIST_SIZE);
}
}
}
if(verbose){outstream.println("tid "+tid+" ran out of input.");}
if(list.size()>0){
putBytes(new ListNum<byte[]>(list, number));
number++;
list=null;
}
if(verbose){outstream.println("tid "+tid+" done reading bytes.");}
putBytes(POISON_BYTES);
if(verbose){outstream.println("tid "+tid+" done poisoning.");}
bf.close();
if(verbose){outstream.println("tid "+tid+" closed stream.");}
}
final void putBytes(ListNum<byte[]> list){
if(verbose){outstream.println("tid "+tid+" putting blist size "+list.size());}
while(list!=null){
try {
inq.put(list);
list=null;
} catch (InterruptedException e) {
// TODO Auto-generated catch block
e.printStackTrace();
}
}
if(verbose){outstream.println("tid "+tid+" done putting blist");}
}
final ListNum<byte[]> takeBytes(){
if(verbose){outstream.println("tid "+tid+" taking blist");}
ListNum<byte[]> list=null;
while(list==null){
try {
list=inq.take();
} catch (InterruptedException e) {
// TODO Auto-generated catch block
e.printStackTrace();
}
}
if(verbose){outstream.println("tid "+tid+" took blist size "+list.size());}
return list;
}
void makeVars(){
if(verbose){outstream.println("tid "+tid+" started makeVars.");}
ListNum<byte[]> list=takeBytes();
ArrayList<Var> vars=new ArrayList<Var>(LIST_SIZE);
while(list!=POISON_BYTES){
vars.clear();
if(vcfMode){
for(byte[] line : list){
assert(line[0]!='#') : new String(line);
Var v=loadVcfLine(line, loadCoverage, extendedInfo);
vars.add(v);
}
}else{
for(byte[] line : list){
assert(line[0]!='#') : new String(line);
Var v=loadVarLine(line);
vars.add(v);
}
}
synchronized(varMap){
for(Var v : vars){
varMap.addUnsynchronized(v);
}
}
list=takeBytes();
}
if(verbose){outstream.println("tid "+tid+" done making vars.");}
putBytes(POISON_BYTES);
if(verbose){outstream.println("tid "+tid+" done poisoning bytes.");}
// putReads(POISON_READS);
// if(verbose){outstream.println("tid "+tid+" done poisoning reads.");}
}
/*--------------------------------------------------------------*/
final ArrayList<ProcessThread> alpt;
final int tid;
final boolean loadCoverage;
final boolean extendedInfo;
boolean success=false;
}
/*--------------------------------------------------------------*/
/*---------------- Final Fields ----------------*/
/*--------------------------------------------------------------*/
/** Primary input file */
final String fname;
final FileFormat ffin;
final ArrayBlockingQueue<ListNum<byte[]>> inq;
final int threads;
ArrayList<byte[]> header=new ArrayList<byte[]>();
/** Print status messages to this output stream */
protected PrintStream outstream=System.err;
final ScafMap scafMap;
final VarMap varMap;
final boolean vcfMode;
boolean errorState=false;
/*--------------------------------------------------------------*/
/*---------------- Static Fields ----------------*/
/*--------------------------------------------------------------*/
static final ListNum<byte[]> POISON_BYTES=new ListNum<byte[]>(null, -1);
static final int LIST_SIZE=200;
public static int DEFAULT_THREADS=3;
static boolean verbose=false;
}
|