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package var2;
import java.io.PrintStream;
import java.util.ArrayList;
import java.util.concurrent.ArrayBlockingQueue;
import fileIO.ByteStreamWriter;
import fileIO.FileFormat;
import gff.GffLine;
import shared.Shared;
import shared.Tools;
import structures.ByteBuilder;
import structures.ListNum;
public class VcfWriter {
/*--------------------------------------------------------------*/
/*---------------- Constructor ----------------*/
/*--------------------------------------------------------------*/
public VcfWriter(VarMap varMap_, VarFilter filter_, long reads_,
long pairs_, long properPairs_, long bases_, String ref_,
boolean trimWhitespace_, String sampleName_){
varMap=varMap_;
filter=filter_;
trimWhitespace=trimWhitespace_;
reads=reads_;
pairs=pairs_;
properPairs=properPairs_;
bases=bases_;
ref=ref_;
sampleName=sampleName_;
ploidy=varMap.ploidy;
properPairRate=varMap.properPairRate;
pairedInSequencingRate=varMap.pairedInSequencingRate;
totalQualityAvg=varMap.totalQualityAvg;
totalMapqAvg=varMap.totalMapqAvg;
readLengthAvg=varMap.readLengthAvg;
scafMap=varMap.scafMap;
threads=Tools.max(1, Shared.threads());
array=varMap.toArray(true);
}
/*--------------------------------------------------------------*/
/*---------------- Outer Methods ----------------*/
/*--------------------------------------------------------------*/
public static boolean writeVcf(String fname, VarMap varMap, VarFilter filter, boolean trimWhitespace,
long reads, long pairs, long properPairs, long bases, String ref, String sampleName){
VcfWriter vw=new VcfWriter(varMap, filter, reads, pairs, properPairs, bases, ref, trimWhitespace, sampleName);
vw.writeVcfFile(fname);
return vw.errorState;
}
public static boolean writeVar(String fname, VarMap varMap, VarFilter filter, boolean trimWhitespace,
long reads, long pairs, long properPairs, long bases, String ref, String sampleName){
VcfWriter vw=new VcfWriter(varMap, filter, reads, pairs, properPairs, bases, ref, trimWhitespace, sampleName);
vw.writeVarFile(fname);
return vw.errorState;
}
public static boolean writeGff(String fname, VarMap varMap, VarFilter filter, boolean trimWhitespace,
long reads, long pairs, long properPairs, long bases, String ref, String sampleName){
VcfWriter vw=new VcfWriter(varMap, filter, reads, pairs, properPairs, bases, ref, trimWhitespace, sampleName);
vw.writeGffFile(fname);
return vw.errorState;
}
public void writeVcfFile(final String fname){
final FileFormat ff=FileFormat.testOutput(fname, FileFormat.VCF, "vcf", true, true, false, true);
writeFile(ff, VCFMODE);
}
public void writeVarFile(final String fname){
final FileFormat ff=FileFormat.testOutput(fname, FileFormat.VAR, "var", true, true, false, true);
writeFile(ff, VARMODE);
}
public void writeGffFile(final String fname){
final FileFormat ff=FileFormat.testOutput(fname, FileFormat.GFF, "gff", true, true, false, true);
writeFile(ff, GFFMODE);
}
public void writeVcfFile(final FileFormat ff){
assert(ff.vcf());
writeFile(ff, VCFMODE);
}
public void writeVarFile(final FileFormat ff){
assert(ff.var()) : "Incorrect file extension: "+ff;
writeFile(ff, VARMODE);
}
public void writeGffFile(final FileFormat ff){
assert(ff.gff());
writeFile(ff, GFFMODE);
}
/*--------------------------------------------------------------*/
/*---------------- Inner Methods ----------------*/
/*--------------------------------------------------------------*/
private void writeFile(final FileFormat ff, final int mode){
assert(ff.ordered());
final ArrayBlockingQueue<ListNum<Var>> inq=new ArrayBlockingQueue<ListNum<Var>>(threads+1);
final ByteStreamWriter bsw=new ByteStreamWriter(ff);
bsw.start();
writeHeader(bsw, mode);
ArrayList<ProcessThread> alpt=spawnThreads(bsw, inq, mode);
makeLists(inq);
waitForFinish(alpt);
errorState=bsw.poisonAndWait()|errorState;
}
private void writeHeader(ByteStreamWriter bsw, int mode){
ByteBuilder bb=new ByteBuilder(1000);
if(mode==VCFMODE){
bb.append(Var.toVcfHeader(properPairRate, totalQualityAvg, totalMapqAvg, filter.rarity, filter.minAlleleFraction,
ploidy, reads, pairs, properPairs, bases, ref, scafMap, sampleName, trimWhitespace)).append('\n');
}else if(mode==VARMODE){
bb.append(Var.toVarHeader(properPairRate, totalQualityAvg, totalMapqAvg, filter.rarity, filter.minAlleleFraction,
ploidy, reads, pairs, properPairs, bases, ref)).append('\n');
}else if(mode==GFFMODE){
bb.append(GffLine.toHeader(properPairRate, totalQualityAvg, totalMapqAvg, filter.rarity, filter.minAlleleFraction,
ploidy, reads, pairs, properPairs, bases, ref)).append('\n');
}else{assert(false);}
bsw.add(bb, 0);
}
/** Spawn process threads */
private ArrayList<ProcessThread> spawnThreads(ByteStreamWriter bsw, final ArrayBlockingQueue<ListNum<Var>> inq, int mode){
//Do anything necessary prior to processing
//Fill a list with ProcessThreads
ArrayList<ProcessThread> alpt=new ArrayList<ProcessThread>(threads);
for(int i=0; i<threads; i++){
alpt.add(new ProcessThread(i, bsw, inq, mode));
}
if(verbose){outstream.println("Spawned threads.");}
//Start the threads
for(ProcessThread pt : alpt){
pt.start();
}
if(verbose){outstream.println("Started threads.");}
//Do anything necessary after processing
return alpt;
}
private void waitForFinish(ArrayList<ProcessThread> alpt){
//Wait for completion of all threads
boolean allSuccess=true;
for(ProcessThread pt : alpt){
if(verbose){outstream.println("Waiting for thread "+pt.tid);}
while(pt.getState()!=Thread.State.TERMINATED){
try {
//Attempt a join operation
pt.join();
} catch (InterruptedException e) {
//Potentially handle this, if it is expected to occur
e.printStackTrace();
}
}
allSuccess&=pt.success;
}
//Track whether any threads failed
if(!allSuccess){errorState=true;}
}
void makeLists(ArrayBlockingQueue<ListNum<Var>> inq){
ArrayList<Var> list=new ArrayList<Var>(LIST_SIZE);
long nextJobID=1;
for(Var v : array){
list.add(v);
if(list.size()>=LIST_SIZE){
putVars(new ListNum<Var>(list, nextJobID), inq);
nextJobID++;
list=new ArrayList<Var>(LIST_SIZE);
}
}
if(list.size()>0){
putVars(new ListNum<Var>(list, nextJobID), inq);
nextJobID++;
list=null;
}
if(verbose){outstream.println("tid "+0+" done making var lists.");}
putVars(POISON_VARS, inq);
if(verbose){outstream.println("tid "+0+" done poisoning.");}
}
final void putVars(ListNum<Var> list, final ArrayBlockingQueue<ListNum<Var>> inq){
if(verbose){outstream.println("tid "+0+" putting vlist "+list.id+", size "+list.size());}
while(list!=null){
try {
inq.put(list);
list=null;
} catch (InterruptedException e) {
// TODO Auto-generated catch block
e.printStackTrace();
}
}
if(verbose){outstream.println("tid "+0+" done putting vlist");}
}
/*--------------------------------------------------------------*/
/*---------------- Nested Classes ----------------*/
/*--------------------------------------------------------------*/
private class ProcessThread extends Thread{
public ProcessThread(int tid_, ByteStreamWriter bsw_, ArrayBlockingQueue<ListNum<Var>> inq_, int mode_){
tid=tid_;
bsw=bsw_;
inq=inq_;
mode=mode_;
}
@Override
public void run(){
makeBytes();
}
void makeBytes(){
if(verbose){outstream.println("tid "+tid+" started makeBytes.");}
ListNum<Var> list=takeVars();
final ByteBuilder bb=new ByteBuilder();
while(list!=null && list.size()>0 && list!=POISON_VARS){
// (filter==null ? true :
// filter.passesFilter(this, properPairRate, totalQualityAvg, mapqAvg, readLengthAvg, ploidy, map, true));
for(Var v : list){
if(v.forced() || filter==null || !filter.failNearby || v.nearbyVarCount<=filter.maxNearbyCount){
if(mode==VCFMODE){
v.toVCF(bb, properPairRate, totalQualityAvg, totalMapqAvg, readLengthAvg, ploidy, scafMap, filter, trimWhitespace);
}else if(mode==VARMODE){
v.toText(bb, properPairRate, totalQualityAvg, totalMapqAvg, readLengthAvg, filter.rarity, ploidy, scafMap);//TODO: Track depth
}else if(mode==GFFMODE){
GffLine.toText(bb, v, properPairRate, totalQualityAvg, totalMapqAvg, readLengthAvg, filter.rarity, ploidy, scafMap);
}
bb.nl();
}
}
bsw.add(new ByteBuilder(bb.toBytes()), list.id);
list=takeVars();
bb.clear();
bb.shrinkTo(SHRINK_SIZE);
}
if(list==POISON_VARS){
putVars(POISON_VARS, inq);
}
if(verbose){outstream.println("tid "+tid+" done making bytes.");}
}
final ListNum<Var> takeVars(){
if(verbose){outstream.println("tid "+tid+" taking vlist");}
ListNum<Var> list=null;
while(list==null){
try {
list=inq.take();
} catch (InterruptedException e) {
// TODO Auto-generated catch block
e.printStackTrace();
}
}
if(verbose){outstream.println("tid "+tid+" took vlist "+list.id+", size "+list.size());}
return list;
}
final ArrayBlockingQueue<ListNum<Var>> inq;
final int tid;
final ByteStreamWriter bsw;
final int mode;
boolean success=false;
}
/*--------------------------------------------------------------*/
/*---------------- Fields ----------------*/
/*--------------------------------------------------------------*/
final Var[] array;
final int ploidy;
final double properPairRate;
final double pairedInSequencingRate;
final double totalQualityAvg;
final double totalMapqAvg;
final double readLengthAvg;
final ScafMap scafMap;
final VarMap varMap;
final VarFilter filter;
final boolean trimWhitespace;
final String sampleName;
final long reads;
final long pairs;
final long properPairs;
final long bases;
final String ref;
final int threads;
boolean errorState=false;
/*--------------------------------------------------------------*/
/*---------------- Static fields ----------------*/
/*--------------------------------------------------------------*/
public static final int VARMODE=0, VCFMODE=1, GFFMODE=2;
private static final ListNum<Var> POISON_VARS=new ListNum<Var>(null, -1);
private static boolean verbose=false;
private static final int LIST_SIZE=200;
private static final int SHRINK_SIZE=1000*LIST_SIZE;
/** Print status messages to this output stream */
protected static PrintStream outstream=System.err;
}
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