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#!/bin/bash
usage(){
echo "
Written by Brian Bushnell
Last modified December 22, 2021
This script requires at least 17GB RAM.
It is designed for NERSC and uses hard-coded paths.
Description: Removes all reads that map to the human genome with at least 88% identity after quality trimming.
This is more aggressive than removehuman.sh and uses an unmasked human genome reference.
It removes roughly 99.99% of human 2x150bp reads, but may incur false-positive removals.
NOTE! This program uses hard-coded paths and will only run on Nersc systems unless you change the path.
Usage: removehuman.sh in=<input file> outu=<clean output file>
Input may be fasta or fastq, compressed or uncompressed.
Parameters:
threads=auto (t) Set number of threads to use; default is number of logical processors.
overwrite=t (ow) Set to false to force the program to abort rather than overwrite an existing file.
interleaved=auto (int) If true, forces fastq input to be paired and interleaved.
trim=t Trim read ends to remove bases with quality below minq.
Values: t (trim both ends), f (neither end), r (right end only), l (left end only).
untrim=t Undo the trimming after mapping.
minq=4 Trim quality threshold.
ziplevel=2 (zl) Set to 1 (lowest) through 9 (max) to change compression level; lower compression is faster.
outm=<file> File to output the reads that mapped to human.
path= Set the path to an indexed human genome.
***** All BBMap parameters can be used; run bbmap.sh for more details. *****
Please contact Brian Bushnell at bbushnell@lbl.gov if you encounter any problems.
"
}
#This block allows symlinked shellscripts to correctly set classpath.
pushd . > /dev/null
DIR="${BASH_SOURCE[0]}"
while [ -h "$DIR" ]; do
cd "$(dirname "$DIR")"
DIR="$(readlink "$(basename "$DIR")")"
done
cd "$(dirname "$DIR")"
DIR="$(pwd)/"
popd > /dev/null
#DIR="$( cd "$( dirname "${BASH_SOURCE[0]}" )" && pwd )/"
CP="$DIR""current/"
JNI="-Djava.library.path=""$DIR""jni/"
JNI=""
z="-Xmx16000m"
z2="-Xms16000m"
set=0
calcXmx () {
source "$DIR""/calcmem.sh"
setEnvironment
parseXmx "$@"
}
calcXmx "$@"
function removehuman() {
local CMD="java $EA $EOOM $z $z2 $JNI -cp $CP align2.BBMap minratio=0.75 maxindel=8 bwr=0.22 bw=26 minhits=1 path=/global/cfs/cdirs/bbtools/hg19 build=2 pigz unpigz zl=6 qtrim=r trimq=10 untrim idtag usemodulo printunmappedcount ztd=2 maxsites=1 k=14 tipsearch=0 kfilter=25 bloomfilter $@"
echo $CMD >&2
eval $CMD
}
removehuman "$@"
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