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#!/bin/bash
usage(){
echo "
Written by Brian Bushnell
Last modified December 22, 2021
This script requires at least 10GB RAM.
It is designed for NERSC and uses hard-coded paths.
Description: Removes all reads that map to selected common microbial contaminant genomes.
Removes approximately 98.5% of common contaminant reads, with zero false-positives to non-bacteria.
NOTE! This program uses hard-coded paths and will only run on Nersc systems.
Usage: removemicrobes.sh in=<input file> outu=<clean output file>
Input may be fasta or fastq, compressed or uncompressed.
Parameters:
in=<file> Input reads. Should already be adapter-trimmed.
outu=<file> Destination for clean reads.
outm=<file> Optional destination for contaminant reads.
threads=auto (t) Set number of threads to use; default is number of logical processors.
overwrite=t (ow) Set to false to force the program to abort rather than overwrite an existing file.
interleaved=auto (int) If true, forces fastq input to be paired and interleaved.
trim=t Trim read ends to remove bases with quality below minq.
Values: t (trim both ends), f (neither end), r (right end only), l (left end only).
untrim=t Undo the trimming after mapping.
minq=4 Trim quality threshold.
ziplevel=6 (zl) Set to 1 (lowest) through 9 (max) to change compression level; lower compression is faster.
build=1 Choses which masking mode was used:
1 is most stringent and should be used for bacteria.
2 uses fewer bacteria for masking (only RefSeq references).
3 is only masked for plastids and entropy, for use on anything except bacteria.
4 is unmasked.
***** All BBMap parameters can be used; run bbmap.sh for more details. *****
Please contact Brian Bushnell at bbushnell@lbl.gov if you encounter any problems.
"
}
#This block allows symlinked shellscripts to correctly set classpath.
pushd . > /dev/null
DIR="${BASH_SOURCE[0]}"
while [ -h "$DIR" ]; do
cd "$(dirname "$DIR")"
DIR="$(readlink "$(basename "$DIR")")"
done
cd "$(dirname "$DIR")"
DIR="$(pwd)/"
popd > /dev/null
#DIR="$( cd "$( dirname "${BASH_SOURCE[0]}" )" && pwd )/"
CP="$DIR""current/"
JNI="-Djava.library.path=""$DIR""jni/"
JNI=""
z="-Xmx6000m"
z2="-Xms6000m"
set=0
if [ -z "$1" ] || [[ $1 == -h ]] || [[ $1 == --help ]]; then
usage
exit
fi
calcXmx () {
source "$DIR""/calcmem.sh"
setEnvironment
parseXmx "$@"
}
calcXmx "$@"
function removemicrobes() {
local CMD="java $EA $EOOM $z $z2 $JNI -cp $CP align2.BBMap strictmaxindel=4 bwr=0.16 bw=12 ef=0.001 minhits=2 path=/global/cfs/cdirs/bbtools/commonMicrobes pigz unpigz zl=6 qtrim=r trimq=10 untrim idtag printunmappedcount ztd=2 kfilter=25 maxsites=1 k=13 minid=0.95 idfilter=0.95 minhits=2 build=1 bloomfilter $@"
echo $CMD >&2
eval $CMD
}
removemicrobes "$@"
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