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#!/bin/bash
usage(){
echo "
Written by Brian Bushnell
Last modified March 3, 2020
Description: Generates basic assembly statistics such as scaffold count,
N50, L50, GC content, gap percent, etc. For multiple files, please use
statswrapper.sh. Works with fasta and fastq only (gzipped is fine).
Please read bbmap/docs/guides/StatsGuide.txt for more information.
Usage: stats.sh in=<file>
Parameters:
in=file Specify the input fasta file, or stdin.
out=stdout Destination of primary output; may be directed to a file.
gc=file Writes ACGTN content per scaffold to a file.
gchist=file Filename to output scaffold gc content histogram.
shist=file Filename to output cumulative scaffold length histogram.
gcbins=200 Number of bins for gc histogram.
n=10 Number of contiguous Ns to signify a break between contigs.
k=13 Estimate memory usage of BBMap with this kmer length.
minscaf=0 Ignore scaffolds shorter than this.
phs=f (printheaderstats) Set to true to print total size of headers.
n90=t (printn90) Print the N/L90 metrics.
extended=f Print additional metrics such as L90, logsum, and score.
pdl=f (printduplicatelines) Set to true to print lines in the
scaffold size table where the counts did not change.
n_=t This flag will prefix the terms 'contigs' and 'scaffolds'
with 'n_' in formats 3-6.
addname=f Adds a column for input file name, for formats 3-6.
Logsum and Powsum:
logoffset=1000 Minimum length for calculating log sum.
logbase=2 Log base for calculating log sum.
logpower=1 Raise the log to a power to increase the weight
of longer scaffolds for log sum.
powsum=0.25 Use this power of the length to increase weight
of longer scaffolds for power sum.
Assembly Score Metric:
score=f Print assembly score.
aligned=0.0 Set the fraction of aligned reads (0-1).
assemblyscoreminlen=2000 Minimum length of scaffolds to include in
assembly score calculation.
assemblyscoremaxlen=50000 Maximum length of scaffolds to get bonus points
for being long.
format=<0-7> Format of the stats information; default 1.
format=0 prints no assembly stats.
format=1 uses variable units like MB and KB, and is designed for compatibility with existing tools.
format=2 uses only whole numbers of bases, with no commas in numbers, and is designed for machine parsing.
format=3 outputs stats in 2 rows of tab-delimited columns: a header row and a data row.
format=4 is like 3 but with scaffold data only.
format=5 is like 3 but with contig data only.
format=6 is like 3 but the header starts with a #.
format=7 is like 1 but only prints contig info.
format=8 is like 3 but in JSON. You can also just use the 'json' flag.
gcformat=<0-5> Select GC output format; default 1.
gcformat=0: (no base content info printed)
gcformat=1: name length A C G T N GC
gcformat=2: name GC
gcformat=4: name length GC
gcformat=5: name length GC logsum powsum
Note that in gcformat 1, A+C+G+T=1 even when N is nonzero.
Please contact Brian Bushnell at bbushnell@lbl.gov if you encounter any problems.
"
}
#This block allows symlinked shellscripts to correctly set classpath.
pushd . > /dev/null
DIR="${BASH_SOURCE[0]}"
while [ -h "$DIR" ]; do
cd "$(dirname "$DIR")"
DIR="$(readlink "$(basename "$DIR")")"
done
cd "$(dirname "$DIR")"
DIR="$(pwd)/"
popd > /dev/null
#DIR="$( cd "$( dirname "${BASH_SOURCE[0]}" )" && pwd )/"
CP="$DIR""current/"
z="-Xmx120m"
set=0
if [ -z "$1" ] || [[ $1 == -h ]] || [[ $1 == --help ]]; then
usage
exit
fi
calcXmx () {
source "$DIR""/calcmem.sh"
setEnvironment
parseXmx "$@"
}
calcXmx "$@"
stats() {
local CMD="java $EA $EOOM $z -cp $CP jgi.AssemblyStats2 $@"
# echo $CMD >&2
eval $CMD
}
stats "$@"
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