1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300 301 302 303 304 305 306 307 308 309 310 311 312 313 314 315 316 317 318 319 320 321 322 323 324 325 326 327
|
/*
* Copyright (C) 2014-2021 Brian L. Browning
*
* This file is part of Beagle
*
* Beagle is free software: you can redistribute it and/or modify
* it under the terms of the GNU General Public License as published by
* the Free Software Foundation, either version 3 of the License, or
* (at your option) any later version.
*
* Beagle is distributed in the hope that it will be useful,
* but WITHOUT ANY WARRANTY; without even the implied warranty of
* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
* GNU General Public License for more details.
*
* You should have received a copy of the GNU General Public License
* along with this program. If not, see <http://www.gnu.org/licenses/>.
*/
package bref;
import beagleutil.ChromIds;
import blbutil.Const;
import blbutil.Filter;
import blbutil.Utilities;
import ints.CharArray;
import ints.IntArray;
import ints.UnsignedByteArray;
import java.io.DataInput;
import java.io.IOException;
import java.util.ArrayList;
import java.util.Arrays;
import java.util.Collection;
import java.util.List;
import vcf.BasicMarker;
import vcf.Marker;
import vcf.RefGTRec;
import vcf.Samples;
import vcf.SeqCodedRefGTRec;
/**
* <p>Class {@code Bref3Reader} contains methods for reading a bref 3
* (binary reference format) file.
* </p>
* <p>Instances of class {@code Bref3Reader} are not thread-safe.
* </p>
* <p>Methods of this class will terminate the Java Virtual Machine with
* an error message if an I/O error or file format error is detected.
* </p>
*
* @author Brian L. Browning {@code <browning@uw.edu>}
*/
public final class Bref3Reader {
static final String READ_ERR = "Error reading file";
private static final String[][] SNV_PERMS = snvPerms();
private final Filter<Marker> markerFilter;
private final String program;
private final Samples samples;
private final int nHaps;
private final byte[] byteBuffer;
/**
* Constructs a new {@code Bref3Reader} instance.
* @param bref a {@code DataInput} instance reading from a bref v3 file
* @param markerFilter a marker filter or {@code null}
*
* @throws IllegalArgumentException if a format error is detected in a
* line of the specified bref v3 file
* @throws NullPointerException if {@code file == null}
*/
public Bref3Reader(DataInput bref, Filter<Marker> markerFilter) {
if (markerFilter == null) {
markerFilter = Filter.acceptAllFilter();
}
String[] sampleIds = null;
String programString = null;
try {
readAndCheckMagicNumber(bref);
programString = readString(bref);
sampleIds = readStringArray(bref);
} catch (IOException ex) {
Utilities.exit(ex, READ_ERR);
}
boolean[] isDiploid = new boolean[sampleIds.length];
Arrays.fill(isDiploid, true);
this.program = programString;
this.samples = Samples.fromIds(sampleIds, isDiploid);
this.markerFilter = markerFilter;
this.nHaps = 2*samples.size();
this.byteBuffer = new byte[2*nHaps];
}
/**
* Returns the list of samples in the brefFile used to construct this
* instance
* @return the list of samples
*/
Samples samples() {
return samples;
}
/**
* Returns the bref file program string in the brefFile used to
* construct this instance
* @return the program string
*/
String program() {
return program;
}
/**
* Returns the marker filter
* @return the marker filter
*/
Filter<Marker> markerFilter() {
return markerFilter;
}
/**
* Reads the next binary reference format data block. The contract for
* this method is undefined if the next byte read from the specified
* {@code DataInput} object is not the first byte of a bref data block
* or is not the first byte of the sentinal denoting no more
* bref data blocks.
* @param bref the bref file and associated file pointer
* @param buffer the collection to which the next block of records
* will be added
* @throws NullPointerException if {@code bref == null || buffer == null}
*/
void readBlock(DataInput bref, Collection<RefGTRec> buffer) {
try {
int nRecs = Integer.MAX_VALUE;
while (buffer.isEmpty() && nRecs!=0) {
nRecs = bref.readInt();
if (nRecs!=0) {
readBlock(bref, buffer, nRecs);
}
}
} catch (IOException ex) {
Utilities.exit(ex, READ_ERR);
}
}
private void readBlock(DataInput bref, Collection<RefGTRec> buffer,
int nRecs) throws IOException {
assert nRecs!= 0;
String chrom = readString(bref);
int chromIndex = ChromIds.instance().getIndex(chrom);
int nSeq = bref.readUnsignedShort();
bref.readFully(byteBuffer);
IntArray hapToSeq = new CharArray(byteBuffer);
for (int j=0; j<nRecs; ++j) {
Marker marker = readMarker(bref, chromIndex);
byte flag = bref.readByte();
if (flag==0) {
RefGTRec rec = readSeqCodedRecord(bref, marker, samples,
hapToSeq, nSeq);
if (markerFilter.accept(marker)) {
buffer.add(rec);
}
}
else if (flag==1) {
RefGTRec rec = readHapCodedRec(bref, marker, samples);
if (markerFilter.accept(marker)) {
buffer.add(rec);
}
}
else {
Utilities.exit(READ_ERR);
}
}
}
private RefGTRec readSeqCodedRecord(DataInput bref, Marker marker,
Samples samples, IntArray hapToSeq, int nSeq) throws IOException {
bref.readFully(byteBuffer, 0, nSeq);
IntArray seqToAllele = new UnsignedByteArray(byteBuffer, 0, nSeq);
// // following code can be uncommented to check data consistency
// if (seqToAllele.max() >= marker.nAlleles()) {
// throw new IllegalArgumentException("inconsistent data");
// }
return new SeqCodedRefGTRec(marker, samples, hapToSeq, seqToAllele);
}
private static void readAndCheckMagicNumber(DataInput di) throws IOException {
int magicNumber=di.readInt();
if (magicNumber!=AsIsBref3Writer.MAGIC_NUMBER_V3) {
String s = "ERROR: Unrecognized input file. Was input file created "
+ Const.nl + "with a different version of the bref program?";
Utilities.exit(s);
}
}
private static Marker readMarker(DataInput di, int chromIndex)
throws IOException {
int pos = di.readInt();
String[] ids = readByteLengthStringArray(di);
byte alleleCode = di.readByte();
if (alleleCode == -1) {
String[] strAlleles = readStringArray(di);
int end = di.readInt();
return new BasicMarker(chromIndex, pos, ids, strAlleles, end);
}
else {
int nAlleles = 1 + (alleleCode & 0b11);
int permIndex = alleleCode >> 2;
String[] strAlleles = alleleString(permIndex, nAlleles);
int end = -1;
return new BasicMarker(chromIndex, pos, ids, strAlleles, end);
}
}
private static RefGTRec readHapCodedRec(DataInput di, Marker marker,
Samples samples) throws IOException {
int nAlleles = marker.nAlleles();
int[][] hapIndices = new int[nAlleles][];
for (int j=0; j<nAlleles; ++j) {
hapIndices[j] = readIntArray(di);
}
return RefGTRec.hapCodedInstance(marker, samples, hapIndices);
}
private static int[] readIntArray(DataInput di) throws IOException {
int length = di.readInt();
if (length == -1) {
return null;
}
else {
int[] ia = new int[length];
byte[] ba = new byte[4*length]; // will overflow if 4*length >= 2^31
di.readFully(ba);
for (int j=0; j<ba.length; j+=4) {
ia[j/4] = (((ba[j] & 0xff) << 24) | ((ba[j+1] & 0xff) << 16) |
((ba[j+2] & 0xff) << 8) | (ba[j+3] & 0xff));
}
return ia;
}
}
private static String readString(DataInput di) throws IOException {
return di.readUTF();
}
private static String[] readByteLengthStringArray(DataInput di)
throws IOException {
int length = di.readUnsignedByte();
return readStringArray(di, length);
}
static String[] readStringArray(DataInput di) throws IOException {
int length = di.readInt();
return readStringArray(di, length);
}
/* Returns null if length is negative */
private static String[] readStringArray(DataInput di, int length)
throws IOException {
if (length<0) {
return null;
} else if (length==0) {
return new String[0];
} else {
String[] sa=new String[length];
for (int j=0; j<sa.length; ++j) {
sa[j]=readString(di);
}
return sa;
}
}
static String[][] snvPerms() {
String[] bases=new String[]{"A", "C", "G", "T"};
List<String[]> perms=new ArrayList<>(24);
permute(new String[0], bases, perms);
return perms.toArray(new String[0][]);
}
private static void permute(String[] start, String[] end,
List<String[]> perms) {
if (end.length==0) {
perms.add(start);
}
else {
for (int j=0; j<end.length; ++j) {
String[] newStart = Arrays.copyOf(start, start.length + 1);
newStart[start.length] = end[j];
String[] newEnd = new String[end.length - 1];
if (j > 0) {
System.arraycopy(end, 0, newEnd, 0, j);
}
if (j < newEnd.length) {
System.arraycopy(end, j+1, newEnd, j, (newEnd.length - j));
}
permute(newStart, newEnd, perms);
}
}
}
/**
* Returns an array that is obtained by taking the first {@code length}
* elements of the specified permutation of "A", "C", "G", and "T".
* The list of 24 permutations of "A", "C", "G", and "T" are sorted
* in lexicographic order.
* @param permIndex an index of a permutation of the bases "A",
* "C", "G", and "T"
* @param length the number of elements in the returned array
* @return an array that is obtained by taking the first {@code length}
* elements of the specified permutation of "A", "C", "G", and "T"
* @throws IndexOutOfBoundsException if
* {@code permIndex < 0 || permIndex >= 24}
* @throws IndexOutOfBoundsException if {@code length < 0 || length >= 4}
*/
private static String[] alleleString(int permIndex, int length) {
return Arrays.copyOf(SNV_PERMS[permIndex], length);
}
@Override
public String toString() {
StringBuilder sb = new StringBuilder(80);
sb.append(this.getClass().toString());
return sb.toString();
}
}
|