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/*
* Copyright (C) 2014-2021 Brian L. Browning
*
* This file is part of Beagle
*
* Beagle is free software: you can redistribute it and/or modify
* it under the terms of the GNU General Public License as published by
* the Free Software Foundation, either version 3 of the License, or
* (at your option) any later version.
*
* Beagle is distributed in the hope that it will be useful,
* but WITHOUT ANY WARRANTY; without even the implied warranty of
* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
* GNU General Public License for more details.
*
* You should have received a copy of the GNU General Public License
* along with this program. If not, see <http://www.gnu.org/licenses/>.
*/
package vcf;
import blbutil.BlockLineReader;
import blbutil.FileIt;
import blbutil.Filter;
import blbutil.SampleFileIt;
import bref.SeqCoder3;
import java.io.File;
import java.util.ArrayDeque;
import java.util.ArrayList;
import java.util.Arrays;
import java.util.Deque;
import java.util.List;
import java.util.NoSuchElementException;
import java.util.function.Function;
/**
* <p>Class {@code RefIt} represents an iterator whose {@code next()}
* method returns an object storing data from a VCF record with
* phased, non-missing genotypes.
* </p>
* <p>Instances of class {@code RefIt} are not thread-safe.
* </p>
* <p>Methods of this class will terminate the Java Virtual Machine with
* an error message if an I/O error or file format error is detected.
* </p>
*
* @author Brian L. Browning {@code <browning@uw.edu>}
*/
public class RefIt implements SampleFileIt<RefGTRec> {
/**
* The default number of {@code GTRec} objects that are
* stored in a buffer.
*/
private static final int DEFAULT_BUFFER_SIZE = 1<<10;
private final VcfHeader vcfHeader;
private final Function<String, RefGTRec> mapper;
private final Filter<Marker> markerFilter;
private final BlockLineReader reader;
private final List<RefGTRec> lowFreqBuffer;
private final Deque<RefGTRec> recBuffer;
private final SeqCoder3 seqCoder;
private final int maxSeqCodedAlleles;
private final int maxSeqCodingMajorCnt;
private int lastChrom = -1;
/**
* Create and returns a new {@code RefIt} instance from the specified
* iterator.
* @param it an iterator that returns lines of a VCF file
* @return a new {@code RefIt} instance
* @throws IllegalArgumentException if a format error is detected in a
* line of a VCF file returned by {@code it}
* @throws NullPointerException if {@code it == null}
*/
public static RefIt create(FileIt<String> it) {
return RefIt.create(it, Filter.acceptAllFilter(),
Filter.acceptAllFilter(), DEFAULT_BUFFER_SIZE);
}
/**
* Create and returns a new {@code RefIt} instance from the specified
* objects.
* @param it an iterator that returns lines of a VCF file
* @param sampleFilter a sample filter or {@code null}
* @param markerFilter a marker filter or {@code null}
* @return a new {@code RefIt} instance
* @throws IllegalArgumentException if a format error is detected in a
* line of a VCF file returned by {@code it}
* @throws IllegalArgumentException if {@code bufferSize < 1}
* @throws NullPointerException if {@code it == null}
*/
public static RefIt create(FileIt<String> it, Filter<String> sampleFilter,
Filter<Marker> markerFilter) {
return new RefIt(it, sampleFilter, markerFilter, DEFAULT_BUFFER_SIZE);
}
/**
* Create and returns a new {@code RefIt} instance from the specified
* objects.
* @param it an iterator that returns lines of a VCF file
* @param sampleFilter a sample filter or {@code null}
* @param markerFilter a marker filter or {@code null}
* @param bufferSize the number of VCF records stored in a buffer
* @return a new {@code RefIt} instance
* @throws IllegalArgumentException if a format error is detected in a
* line of a VCF file returned by {@code it}
* @throws IllegalArgumentException if {@code bufferSize < 1}
* @throws NullPointerException if {@code it == null}
*/
public static RefIt create(FileIt<String> it, Filter<String> sampleFilter,
Filter<Marker> markerFilter, int bufferSize) {
return new RefIt(it, sampleFilter, markerFilter, bufferSize);
}
private RefIt(FileIt<String> it, Filter<String> sampleFilter,
Filter<Marker> markerFilter, int blockSize) {
if (blockSize < 1) {
throw new IllegalArgumentException(String.valueOf(blockSize));
}
if (markerFilter==null) {
markerFilter = Filter.acceptAllFilter();
}
String src = it.file()==null ? "stdin" : it.file().getName();
String[] head = VcfIt.head(src, it);
String[] nonDataLines = Arrays.copyOf(head, head.length-1);
String firstDataLine = head[head.length-1];
boolean[] isDiploid = VcfHeader.isDiploid(firstDataLine);
this.vcfHeader = new VcfHeader(src, nonDataLines, isDiploid, sampleFilter);
this.mapper = (String s) -> {
return RefGTRec.alleleCodedInstance(new VcfRecGTParser(vcfHeader, s));
} ;
this.markerFilter = markerFilter;
this.seqCoder = new SeqCoder3(vcfHeader.samples());
this.maxSeqCodedAlleles = Math.min(seqCoder.maxNSeq(), SeqCoder3.MAX_NALLELES);
this.maxSeqCodingMajorCnt = maxSeqCodingMajorCnt(vcfHeader.samples());
this.lowFreqBuffer = new ArrayList<>();
this.recBuffer = new ArrayDeque<>(blockSize);
int nBlocks = 1;
this.reader = BlockLineReader.create(it, blockSize, nBlocks);
fillRecBuffer(firstDataLine);
}
private int maxSeqCodingMajorCnt(Samples samples) {
int nHaps = samples.size() << 1;
return (int) Math.floor(nHaps*SeqCoder3.COMPRESS_FREQ_THRESHOLD - 1);
}
private void fillRecBuffer() {
fillRecBuffer(null);
}
private void fillRecBuffer(String firstDataLine) {
assert recBuffer.isEmpty();
while (recBuffer.isEmpty()) {
String[] lines = reader.next();
if (firstDataLine!=null) {
lines = combine(firstDataLine, lines);
firstDataLine = null;
}
if (lines==BlockLineReader.SENTINAL) {
flushCompressedRecords();
return;
}
else {
RefGTRec[] recs = parseLines(lines);
for (int j=0; j<recs.length; ++j) {
RefGTRec rec = recs[j];
int chrom = rec.marker().chromIndex();
if (lastChrom == -1) {
lastChrom = chrom;
}
if (chrom!=lastChrom || lowFreqBuffer.size()==Integer.MAX_VALUE) {
flushCompressedRecords();
lastChrom = chrom;
}
if (applySeqCoding(rec)==false) {
lowFreqBuffer.add(rec);
}
else {
boolean success = seqCoder.add(rec);
if (success == false) {
flushCompressedRecords();
success = seqCoder.add(rec);
assert success;
}
lowFreqBuffer.add(null);
}
}
}
}
}
private String[] combine(String firstDataLine, String[] lines) {
String[] modLines = new String[lines.length + 1];
modLines[0] = firstDataLine;
System.arraycopy(lines, 0, modLines, 1, lines.length);
return modLines;
}
private RefGTRec[] parseLines(String[] lines) {
return Arrays.stream(lines)
.parallel()
.map(mapper)
.filter(e -> markerFilter.accept(e.marker()))
.toArray(RefGTRec[]::new);
}
private void flushCompressedRecords() {
List<RefGTRec> list = seqCoder.getCompressedList();
int index = 0;
for (int j=0, n=lowFreqBuffer.size(); j<n; ++j) {
GTRec ve = lowFreqBuffer.get(j);
if (ve==null) {
lowFreqBuffer.set(j, list.get(index++));
}
}
recBuffer.addAll(lowFreqBuffer);
lowFreqBuffer.clear();
}
@Override
public void close() {
reader.close();
recBuffer.clear();
lowFreqBuffer.clear();
}
/**
* Returns {@code true} if the iteration has more elements, and returns
* {@code false} otherwise.
* @return {@code true} if the iteration has more elements
*/
@Override
public boolean hasNext() {
return !recBuffer.isEmpty();
}
/**
* Returns the next element in the iteration.
* @return the next element in the iteration
* @throws NoSuchElementException if the iteration has no more elements
*/
@Override
public RefGTRec next() {
if (!hasNext()) {
throw new NoSuchElementException();
}
RefGTRec first = recBuffer.removeFirst();
if (recBuffer.isEmpty()) {
fillRecBuffer();
}
return first;
}
/**
* The {@code remove} method is not supported by this iterator.
* @throws UnsupportedOperationException if this method is invoked
*/
@Override
public void remove() {
throw new UnsupportedOperationException(this.getClass().toString());
}
@Override
public File file() {
return reader.file();
}
@Override
public Samples samples() {
return vcfHeader.samples();
}
@Override
public String toString() {
File file = reader.file();
StringBuilder sb = new StringBuilder(80);
sb.append(this.getClass().toString());
sb.append(" : ");
sb.append(file==null ? "stdin" : file.toString());
return sb.toString();
}
private boolean applySeqCoding(RefGTRec rec) {
assert rec.isAlleleCoded();
if (rec.marker().nAlleles() > maxSeqCodedAlleles) {
return false;
}
int nHaps = rec.size();
int majAllele = rec.majorAllele();
int majCnt = nHaps;
for (int a=0, n=rec.marker().nAlleles(); a<n; ++a) {
if (a!=majAllele) {
majCnt -= rec.alleleCount(a);
}
}
return majCnt<=maxSeqCodingMajorCnt;
}
}
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