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/*
* Copyright (C) 2014-2021 Brian L. Browning
*
* This file is part of Beagle
*
* Beagle is free software: you can redistribute it and/or modify
* it under the terms of the GNU General Public License as published by
* the Free Software Foundation, either version 3 of the License, or
* (at your option) any later version.
*
* Beagle is distributed in the hope that it will be useful,
* but WITHOUT ANY WARRANTY; without even the implied warranty of
* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
* GNU General Public License for more details.
*
* You should have received a copy of the GNU General Public License
* along with this program. If not, see <http://www.gnu.org/licenses/>.
*/
package vcf;
import blbutil.FileIt;
import blbutil.Filter;
import blbutil.SampleFileIt;
import java.io.File;
import java.util.ArrayDeque;
import java.util.ArrayList;
import java.util.Arrays;
import java.util.Deque;
import java.util.List;
import java.util.NoSuchElementException;
import java.util.function.BiFunction;
import java.util.function.Function;
import java.util.stream.Collectors;
import java.util.stream.IntStream;
/**
* <p>Class {@code VcfIt} represents an iterator whose {@code next()}
* method returns an object storing data from a VCF record.
* </p>
* <p>Instances of class {@code VcfIt} are not thread-safe.
* </p>
* <p>Methods of this class will terminate the Java Virtual Machine with
* an error message if an I/O error or file format error is detected.
* </p>
* @param <E> the type parameter
*
* @author Brian L. Browning {@code <browning@uw.edu>}
*/
public class VcfIt<E extends GTRec> implements SampleFileIt<E> {
private static final float DEFAULT_MAX_LR = Float.MAX_VALUE;
private final VcfHeader vcfHeader;
private final FileIt<String> it;
private final Function<String, E> mapper;
private String next;
private final Filter<Marker> markerFilter;
private final int bufferSize;
private final String[] stringBuffer;
private final Deque<E> recBuffer;
/**
* The default number of VCF records stored in a buffer.
*/
public static final int DEFAULT_BUFFER_SIZE = 1<<10;
/**
* A function mapping a string VCF record with GT format fields
* to a memory-efficient {@code GTRec} object. Phase status is stored
* per record. All genotypes are considered to be unphased if any
* genotype is unphased or if any allele is missing.
*/
public static final BiFunction<VcfHeader, String, GTRec> TO_LOWMEM_GT_REC
= (VcfHeader h, String s) -> {
VcfRecGTParser.HapListRep hlr = new VcfRecGTParser(h, s)
.hapListRep();
int nonMajorAlleleThreshold = (hlr.samples().size()>>7);
if (hlr.nonmajorAlleleCnt()<=nonMajorAlleleThreshold) {
if (hlr.marker().nAlleles()==2) {
LowMafDiallelicGTRec rec = new LowMafDiallelicGTRec(hlr);
return rec;
}
else {
LowMafGTRec rec = new LowMafGTRec(hlr);
return rec;
}
}
else {
BitArrayGTRec rec = new BitArrayGTRec(hlr);
return rec;
}
};
/**
* A function mapping a string VCF record with GT format fields
* to a {@code GTRec} object. Phase status is stored per-genotype.
*/
public static final BiFunction<VcfHeader, String, GTRec> TO_BASIC_GT_REC
= (VcfHeader h, String s) -> new BasicGTRec(new VcfRecGTParser(h, s));
/**
* A function mapping a string VCF record with GT or GL format fields
* to a {@code VcfRecord} object.
*/
public static final BiFunction<VcfHeader, String, VcfRec> TO_VCF_REC
= (VcfHeader h, String s) -> VcfRec.fromGTGL(h, s, DEFAULT_MAX_LR);
/**
* Returns an array containing VCF meta-information lines, the
* VCF header line, and the first VCF data line.
* @param src a string describing the source of the VCF file
* @param it an iterator that returns the lines of a VCF file
* @return an array containing VCF meta-information lines, the
* VCF header line, and the first VCF data line
* @throws NullPointerException if {@code it == null}
* @throws IllegalArgumentException if all lines returned by the iterator
* begin with the '#' character
*/
static String[] head(String src, FileIt<String> it) {
String hash = "#";
List<String> lines = new ArrayList<>(32);
String line = it.hasNext() ? it.next() : null;
while (line!=null && line.startsWith(hash)) {
lines.add(line);
line = it.hasNext() ? it.next() : null;
}
if (line==null) {
throw new IllegalArgumentException("ERROR: missing VCF data lines ("
+ src + ")");
}
lines.add(line);
return lines.toArray(new String[0]);
}
/**
* Create and returns a new {@code VcfIt} instance from the specified
* objects.
* @param <R> the type returned by the returned {@code VcfIt}
* @param strIt an iterator that returns lines of a VCF file
* @param recMapper a function mapping string VCF records to
* {@code GTRec} objects
* @return a new {@code VcfIt} instance
* @throws IllegalArgumentException if a format error is detected in a
* line of a VCF file returned by {@code strIt}
* @throws NullPointerException if
* {@code strIt == null || mapFactory == null}
*/
public static <R extends GTRec> VcfIt<R> create(
FileIt<String> strIt, BiFunction<VcfHeader, String, R> recMapper) {
return VcfIt.create(strIt, Filter.acceptAllFilter(),
Filter.acceptAllFilter(), recMapper);
}
/**
* Create and returns a new {@code VcfIt} instance from the specified
* objects.
* @param <R> the type returned by the returned {@code VcfIt}
* @param strIt an iterator that returns lines of a VCF file
* @param sampleFilter a sample filter or {@code null}
* @param markerFilter a marker filter or {@code null}
* @param recMapper a function mapping string VCF records to
* {@code GTRec} objects
* @return a new {@code VcfIt} instance
* @throws IllegalArgumentException if a format error is detected in a
* line of a VCF file returned by {@code strIt}
* @throws NullPointerException if
* {@code strIt == null || mapFactory == null}
*/
public static <R extends GTRec> VcfIt<R> create(
FileIt<String> strIt, Filter<String> sampleFilter,
Filter<Marker> markerFilter,
BiFunction<VcfHeader, String, R> recMapper) {
return VcfIt.create(strIt, sampleFilter, markerFilter, recMapper,
DEFAULT_BUFFER_SIZE);
}
/**
* Create and returns a new {@code VcfIt} instance from the specified
* objects.
* @param <R> the type returned by the returned {@code VcfIt}
* @param strIt an iterator that returns lines of a VCF file
* @param sampleFilter a sample filter or {@code null}
* @param markerFilter a marker filter or {@code null}
* @param recMapper a function mapping string VCF records to
* {@code GTRec} objects
* @param bufferSize the requested buffer size
* @return a new {@code VcfIt} instance
* @throws IllegalArgumentException if a format error is detected in a
* line of a VCF file returned by {@code strIt}
* @throws IllegalArgumentException if {@code bufferSize < 1}
* @throws NullPointerException if
* {@code strIt == null || mapFactory == null}
*/
public static <R extends GTRec> VcfIt<R> create(
FileIt<String> strIt, Filter<String> sampleFilter,
Filter<Marker> markerFilter,
BiFunction<VcfHeader, String, R> recMapper, int bufferSize) {
return new VcfIt<>(strIt, sampleFilter, markerFilter,
recMapper, bufferSize);
}
private VcfIt(FileIt<String> it, Filter<String> sampleFilter,
Filter<Marker> markerFilter,
BiFunction<VcfHeader, String, E> recMapper,
int bufferSize) {
if (bufferSize < 1) {
throw new IllegalArgumentException(String.valueOf(bufferSize));
}
if (markerFilter==null) {
markerFilter = Filter.acceptAllFilter();
}
String src = it.file()==null ? "stdin" : it.file().getName();
String[] head = head(src, it);
String[] nonDataLines = Arrays.copyOf(head, head.length-1);
String firstDataLine = head[head.length-1];
boolean[] isDiploid = VcfHeader.isDiploid(firstDataLine);
this.it = it;
this.vcfHeader = new VcfHeader(src, nonDataLines, isDiploid, sampleFilter);
this.mapper = (String s) -> recMapper.apply(vcfHeader, s);
this.next = firstDataLine;
this.markerFilter = markerFilter;
this.bufferSize = bufferSize;
this.stringBuffer = new String[stringBufferSize(next, bufferSize)];
this.recBuffer = new ArrayDeque<>(bufferSize);
fillEmissionBuffer();
}
private static int stringBufferSize(String line, int maxBufferSize) {
long maxMem = Runtime.getRuntime().maxMemory();
long nBytesPerLine = 2*(line==null ? 0 : line.length());
long bufferSize = 1 + (maxMem >> 4)/(nBytesPerLine);
return (int) Math.min(bufferSize, maxBufferSize);
}
private void fillEmissionBuffer() {
assert recBuffer.isEmpty();
int size = -1;
while (size!=0 && recBuffer.size()<bufferSize) {
size = fillStringBuffer(stringBuffer.length);
if (size>0) {
List<E> list = IntStream.range(0, size)
.parallel()
.mapToObj(j -> stringBuffer[j])
.map(mapper)
.filter(e -> markerFilter.accept(e.marker()))
.collect(Collectors.toList());
recBuffer.addAll(list);
}
}
}
private int fillStringBuffer(int maxSize) {
int size = 0;
if (next != null) {
while (next!=null && size<maxSize) {
stringBuffer[size++] = next;
next = readLine(it);
}
}
return size;
}
private static String readLine(FileIt<String> it) {
if (it.hasNext()==false) {
return null;
}
String line = it.next();
while (line.trim().isEmpty() && it.hasNext()) {
line = it.next();
}
return line;
}
@Override
public void close() {
next = null;
it.close();
recBuffer.clear();
Arrays.fill(stringBuffer, null);
}
/**
* Returns {@code true} if the iteration has more elements, and returns
* {@code false} otherwise.
* @return {@code true} if the iteration has more elements
*/
@Override
public boolean hasNext() {
return !recBuffer.isEmpty();
}
/**
* Returns the next element in the iteration.
* @return the next element in the iteration
* @throws NoSuchElementException if the iteration has no more elements.
*/
@Override
public E next() {
if (!hasNext()) {
throw new NoSuchElementException();
}
E first = recBuffer.removeFirst();
if (recBuffer.isEmpty()) {
fillEmissionBuffer();
}
return first;
}
/**
* The {@code remove} method is not supported by this iterator.
* @throws UnsupportedOperationException if this method is invoked
*/
@Override
public void remove() {
throw new UnsupportedOperationException(this.getClass().toString());
}
@Override
public File file() {
return it.file();
}
@Override
public Samples samples() {
return vcfHeader.samples();
}
@Override
public String toString() {
StringBuilder sb = new StringBuilder(80);
sb.append(this.getClass().toString());
sb.append(" : ");
sb.append(it.file()==null ? "stdin" : it.file().toString());
return sb.toString();
}
}
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