1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297
|
/*
* Copyright (C) 2014-2021 Brian L. Browning
*
* This file is part of Beagle
*
* Beagle is free software: you can redistribute it and/or modify
* it under the terms of the GNU General Public License as published by
* the Free Software Foundation, either version 3 of the License, or
* (at your option) any later version.
*
* Beagle is distributed in the hope that it will be useful,
* but WITHOUT ANY WARRANTY; without even the implied warranty of
* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
* GNU General Public License for more details.
*
* You should have received a copy of the GNU General Public License
* along with this program. If not, see <http://www.gnu.org/licenses/>.
*/
package vcf;
import blbutil.Const;
import java.io.PrintWriter;
import java.text.SimpleDateFormat;
import java.util.Calendar;
import java.util.stream.IntStream;
/**
* <p>Class {@code VcfWriter} contains static methods for writing data in
* VCF 4.2 format.
* </p>
* <p>Instances of class {@code VcfWriter} are not thread-safe.
* </p>
*
* @author Brian L. Browning {@code <browning@uw.edu>}
*/
public final class VcfWriter {
private static final String FILE_FORMAT = "##fileformat=VCFv4.2";
private static final String AF_INFO = "##INFO=<ID=AF,Number=A,Type=Float,"
+ "Description=\"Estimated ALT Allele Frequencies\">";
private static final String DR2_INFO = "##INFO=<ID=DR2,Number=A,Type=Float,"
+ "Description=\"Dosage R-Squared: estimated squared correlation between "
+ "estimated REF dose [P(RA) + 2*P(RR)] and true REF dose\">";
private static final String IMP_INFO = "##INFO=<ID=IMP,Number=0,Type=Flag,"
+ "Description=\"Imputed marker\">";
private static final String GT_FORMAT = "##FORMAT=<ID=GT,Number=1,Type=String,"
+ "Description=\"Genotype\">";
private static final String DS_FORMAT = "##FORMAT=<ID=DS,Number=A,Type=Float,"
+"Description=\"estimated ALT dose [P(RA) + 2*P(AA)]\">";
private static final String AP1_FORMAT = "##FORMAT=<ID=AP1,Number=A,Type=Float,"
+"Description=\"estimated ALT dose on first haplotype\">";
private static final String AP2_FORMAT = "##FORMAT=<ID=AP2,Number=A,Type=Float,"
+"Description=\"estimated ALT dose on second haplotype\">";
private static final String GL_FORMAT = "##FORMAT=<ID=GL,Number=G,Type=Float,"
+ "Description=\"Log10-scaled Genotype Likelihood\">";
private static final String GP_FORMAT = "##FORMAT=<ID=GP,Number=G,Type=Float,"
+ "Description=\"Estimated Genotype Probability\">";
private static final String SHORT_CHROM_PREFIX= "#CHROM" + Const.tab + "POS"
+ Const.tab + "ID" + Const.tab + "REF" + Const.tab + "ALT"
+ Const.tab + "QUAL" + Const.tab + "FILTER" + Const.tab + "INFO";
private static final String LONG_CHROM_PREFIX =
SHORT_CHROM_PREFIX + Const.tab + "FORMAT";
private VcfWriter() {
// private constructor prevents instantiation
}
/**
* Writes VCF meta-information lines and header line to the specified
* {@code PrintWriter}. Only one FORMAT subfield, the GT subfield,
* is described in the meta-information lines.
* @param sampleIds the sample identifiers
* @param source a description of the data source, or {@code null} if
* no description is to be printed
* @param out the {@code PrintWriter} to which VCF meta-information
* lines will be written
* @throws NullPointerException if {@code out == null}
* @throws NullPointerException if
* {@code sampleIds == null}, or if {@code sampleIds[j] == null} for any
* {@code j} satisfying {@code (0 <= j && j < <sampleIds.length)}
*/
public static void writeMetaLinesGT(String[] sampleIds, String source,
PrintWriter out) {
boolean ds = false;
boolean ap = false;
boolean gp = false;
boolean gl = false;
writeMetaLines(sampleIds, source, ds, ap, gp, gl, out);
}
/**
* Writes VCF meta-information lines and header line to the specified
* {@code PrintWriter}.
* @param sampleIds the sample identifiers
* @param source a description of the data source, or {@code null} if
* no description is to be printed
* @param ds{ @code true} if the meta-information lines
* will describe the DS FORMAT subfield and {@code false} otherwise
* @param ap {@code true} if the meta-information lines
* will describe the AP1 and AP2 FORMAT subfields and {@code false} otherwise
* @param gp {@code true} if the meta-information lines
* will describe the GP FORMAT subfield and {@code false} otherwise
* @param gl {@code true} if the meta-information lines
* will describe the GL FORMAT subfield and {@code false} otherwise
* @param out the {@code PrintWriter} to which VCF meta-information lines
* will be written.
* @throws NullPointerException if {@code out == null}
* @throws NullPointerException if
* {@code sampleIds == null}, or if {@code sampleIds[j] == null} for any
* {@code j} satisfying {@code (0 <= j && j < sampleIds.length)}
*/
public static void writeMetaLines(String[] sampleIds, String source,
boolean ds, boolean ap, boolean gp, boolean gl,
PrintWriter out) {
out.print(FILE_FORMAT);
out.print(Const.nl);
out.print("##filedate=");
out.print(now());
out.print(Const.nl);
if (source != null) {
out.print("##source=\"");
out.print(source);
out.println("\"");
}
if (ds) {
out.println(AF_INFO);
out.println(DR2_INFO);
out.println(IMP_INFO);
}
out.println(GT_FORMAT);
if (ds) {
out.println(DS_FORMAT);
}
if (ap) {
out.println(AP1_FORMAT);
out.println(AP2_FORMAT);
}
if (gp) {
out.println(GP_FORMAT);
}
if (gl) {
out.println(GL_FORMAT);
}
out.print(LONG_CHROM_PREFIX);
for (String id : sampleIds) {
if (id==null) {
throw new NullPointerException("id==null");
}
out.print(Const.tab);
out.print(id);
}
out.println();
}
private static String now() {
String dateFormat = "yyyyMMdd";
Calendar cal = Calendar.getInstance();
SimpleDateFormat sdf = new SimpleDateFormat(dateFormat);
return sdf.format(cal.getTime());
}
/**
* Writes the data in phased genotypes for the specified markers
* to the specified {@code PrintWriter}.
* @param phasedTarg the estimated haplotype allele probabilities
* @param start the starting marker index (inclusive)
* @param end the ending marker index (exclusive)
* @param out the {@code PrintWriter} to which VCF records will be written
* @throws IllegalArgumentException if
* {@code phasedTarg.isPhased() == false}
* @throws IndexOutOfBoundsException if
* {@code (start < 0 || start > end || end > phasedTarg.nMarkers())}
* @throws NullPointerException if
* {@code phasedTarg == null || out == null}
*/
public static void appendRecords(GT phasedTarg, int start, int end,
PrintWriter out) {
if (start > end) {
throw new IllegalArgumentException("start=" + start + " end=" + end);
}
if (phasedTarg.isPhased()==false) {
throw new IllegalArgumentException("unphased genotypes");
}
Samples samples = phasedTarg.samples();
Markers markers = phasedTarg.markers();
VcfRecBuilder[] recBuilders = recBuilders(markers, samples.size(), start, end);
for (int s=0, n=phasedTarg.nSamples(); s<n; ++s) {
if (samples.isDiploid(s)) {
int h1 = s<<1;
int h2 = h1 | 0b1;
for (int m=start; m<end; ++m) {
int a1 = phasedTarg.allele(m, h1);
int a2 = phasedTarg.allele(m, h2);
recBuilders[m-start].addSampleData(a1, a2);
}
}
else {
for (int m=start; m<end; ++m) {
int a1 = phasedTarg.allele(m, (s<<1));
recBuilders[m-start].addSampleData(a1);
}
}
}
for (VcfRecBuilder vrb : recBuilders) {
vrb.writeRec(out);
}
}
/**
* Writes the data in phased genotypes for the specified markers
* to the specified {@code PrintWriter}.
* @param samples the list of samples
* @param recs the estimated haplotype allele probabilities
* @param out the {@code PrintWriter} to which VCF records will be written
* @throws IllegalArgumentException if
* {@code phasedTarg.isPhased() == false}
* @throws IndexOutOfBoundsException if
* {@code (start < 0 || start > end || end > phasedTarg.nMarkers())}
* @throws NullPointerException if
* {@code phasedTarg == null || out == null}
*/
public static void appendRecords(Samples samples, GTRec[] recs,
PrintWriter out) {
VcfRecBuilder[] recBuilders = recBuilders(samples, recs);
int nSamples = samples.size();
for (int s=0; s<nSamples; ++s) {
if (samples.isDiploid(s)) {
int h1 = s<<1;
int h2 = h1 | 0b1;
for (int j=0; j<recBuilders.length; ++j) {
int a1 = recs[j].get(h1);
int a2 = recs[j].get(h2);
recBuilders[j].addSampleData(a1, a2);
}
}
else {
for (int j=0; j<recBuilders.length; ++j) {
int a1 = recs[j].get(s<<1);
recBuilders[j].addSampleData(a1);
}
}
}
for (VcfRecBuilder vrb : recBuilders) {
vrb.writeRec(out);
}
}
private static VcfRecBuilder[] recBuilders(Samples samples, GTRec[] recs) {
int nSamples = samples.size();
VcfRecBuilder[] builders = new VcfRecBuilder[recs.length];
for (int j=0; j<recs.length; ++j) {
if (recs[j].isPhased()==false) {
throw new IllegalArgumentException("unphased genotypes");
}
else if (recs[j].samples().equals(samples)==false) {
throw new IllegalArgumentException("inconsistent samples");
}
builders[j] = new VcfRecBuilder(recs[j].marker(), nSamples);
}
return builders;
}
private static VcfRecBuilder[] recBuilders(Markers markers, int nSamples,
int start, int end) {
return IntStream.range(start, end)
.mapToObj(m -> new VcfRecBuilder(markers.marker(m), nSamples))
.toArray(VcfRecBuilder[]::new);
}
/**
* Prints the first 9 VCF record fields for the specified marker to
* the specified {@code PrintWriter}. Only one VCF FORMAT subfield,
* the GT subfield, is printed.
*
* @param marker a marker
* @param out the {@code PrintWriter} to which the first 9 VCF record
* fields will be written
*
* @throws NullPointerException if {@code marker == null || out == null}
*/
public static void printFixedFieldsGT(Marker marker, PrintWriter out) {
out.print(marker);
out.print(Const.tab);
out.print(Const.MISSING_DATA_CHAR); // QUAL
out.print(Const.tab);
out.print("PASS"); // FILTER
out.print(Const.tab);
out.print(Const.MISSING_DATA_CHAR); // INFO
out.print(Const.tab);
out.print("GT");
}
}
|