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/*
* Copyright (C) 2014-2021 Brian L. Browning
*
* This file is part of Beagle
*
* Beagle is free software: you can redistribute it and/or modify
* it under the terms of the GNU General Public License as published by
* the Free Software Foundation, either version 3 of the License, or
* (at your option) any later version.
*
* Beagle is distributed in the hope that it will be useful,
* but WITHOUT ANY WARRANTY; without even the implied warranty of
* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
* GNU General Public License for more details.
*
* You should have received a copy of the GNU General Public License
* along with this program. If not, see <http://www.gnu.org/licenses/>.
*/
package main;
import blbutil.BGZIPOutputStream;
import blbutil.Utilities;
import imp.ImpData;
import imp.ImputedVcfWriter;
import imp.StateProbs;
import ints.IntList;
import ints.UnsignedByteArray;
import java.io.BufferedOutputStream;
import java.io.ByteArrayOutputStream;
import java.io.Closeable;
import java.io.File;
import java.io.FileOutputStream;
import java.io.IOException;
import java.io.OutputStream;
import java.io.PrintWriter;
import java.util.Arrays;
import java.util.concurrent.atomic.AtomicReferenceArray;
import java.util.stream.IntStream;
import phase.Stage2Haps;
import vcf.GT;
import vcf.GTRec;
import vcf.Samples;
import vcf.VcfWriter;
/**
* <p>Class {@code WindowWriter} writes VCF and IBD output data.
* </p>
* <p>Instances of class {@code WindowWriter} are not thread-safe.
* </p>
*
* @author Brian L. Browning {@code <browning@uw.edu>}
*/
public class WindowWriter implements Closeable {
private final Samples samples;
private final String outPrefix;
private final File vcfOutFile;
/**
* Constructs a new {@code WindowWriter} object.
* @param par the analysis parameters
* @param samples the sample whose data will be printed
*
* @throws IllegalArgumentException if {@code outPrefix.length() == 0}
* @throws NullPointerException if
* {@code par == null || samples == null}
*/
public WindowWriter(Par par, Samples samples) {
if (samples==null) {
throw new NullPointerException("samples==null");
}
this.outPrefix = par.out();
this.vcfOutFile = new File(outPrefix + ".vcf.gz");
this.samples = samples;
ByteArrayOutputStream baos = new ByteArrayOutputStream();
try (PrintWriter vcfOut=new PrintWriter(
new BGZIPOutputStream(baos, false))) {
boolean ds = true;
boolean ap = par.ap();
boolean gp = par.gp();
boolean gl = false;
VcfWriter.writeMetaLines(samples.ids(), Main.PROGRAM,
ds, ap, gp, gl, vcfOut);
}
try {
try (FileOutputStream fos=new FileOutputStream(vcfOutFile)) {
fos.write(baos.toByteArray());
}
} catch (IOException e) {
fileOutputError(vcfOutFile, e);
}
}
/**
* Returns the output file prefix.
* @return the output file prefix
*/
public String outPrefix() {
return outPrefix;
}
/**
* Returns the samples whose data is written by {@code this}.
* @return the samples whose data is written by {@code this}
*/
public Samples samples() {
return samples;
}
/**
* Prints the data in {@code alProbs} for markers
* with index between {@code refStart} (inclusive) and
* {@code refEnd} (exclusive) to the output
* VCF file: {@code this.outPrefix() + ".vcf.gz"}.
*
* @param impData the input data for genotype imputation
* @param stateProbs the imputed state probabilities
* @param start the starting reference marker index (inclusive)
* @param end the ending reference marker index (exclusive)
*
* @throws IllegalArgumentException if
* {@code stateProbs.size() != impData.nTargHaps()}
* @throws IndexOutOfBoundsException if
* {@code start < 0 || end > impData.refGT().nMarkers()}
* @throws NullPointerException if {@code impData==null || stateProbs==null}
* @throws NullPointerException if any element of {@code stateProbs} is
* {@code null}
*/
public void printImputed(ImpData impData, int start, int end,
AtomicReferenceArray<StateProbs> stateProbs) {
checkInterval(start, end, impData.refGT().nMarkers());
if (stateProbs.length() != impData.nTargHaps()) {
throw new IllegalArgumentException("inconsistent data:");
}
UnsignedByteArray[] output = IntStream.range(0, impData.nClusters())
.parallel()
.mapToObj(c -> toByteArray(impData, start, end, stateProbs, c))
.toArray(UnsignedByteArray[]::new);
append(output, vcfOutFile);
}
private static UnsignedByteArray toByteArray(ImpData impData,
int refStart, int refEnd,
AtomicReferenceArray<StateProbs> stateProbs, int m) {
ImputedVcfWriter ivw = new ImputedVcfWriter(impData, refStart, refEnd, m);
ByteArrayOutputStream baos = new ByteArrayOutputStream();
try (PrintWriter out = new PrintWriter(
new BGZIPOutputStream(baos, false))) {
ivw.appendRecords(stateProbs, out);
}
return new UnsignedByteArray(baos);
}
/**
* Appends the data in phased genotypes for the specified markers
* to the output VCF file: {@code this.outPrefix() + ".vcf.gz"}.
*
* @param phasedTarg the estimated target haplotypes
* @param start the starting marker index (inclusive)
* @param end the ending marker index (exclusive)
* @throws IllegalArgumentException if {@code phasedTarg.isPhased() == false}
* @throws IndexOutOfBoundsException if
* {@code start < 0 || end > phasedTarg.nMarkers() || start > end}
* @throws NullPointerException if {@code phasedTarg == null}
*/
public void printPhased(GT phasedTarg, int start, int end) {
checkInterval(start, end, phasedTarg.nMarkers());
long t0 = System.nanoTime();
int blockSize = 50000;
int stepSize = 100;
int[] blockEnds = ends(start, end, blockSize);
for (int j=1; j<blockEnds.length; ++j) {
int[] stepEnds = ends(blockEnds[j-1], blockEnds[j], stepSize);
UnsignedByteArray[] output = IntStream.range(1, stepEnds.length)
.parallel()
.mapToObj(i -> toByteArray(phasedTarg, stepEnds[i-1], stepEnds[i]))
.toArray(UnsignedByteArray[]::new);
append(output, vcfOutFile);
}
long t1 = System.nanoTime();
System.out.println("WindWriter tot: " + Utilities.elapsedNanos(t1-t0));
}
public void printPhased(Stage2Haps stage2Haps, int start, int end) {
GT targGT = stage2Haps.fpd().targGT();
checkInterval(start, end, targGT.nMarkers());
long t0 = System.nanoTime();
int blockSize = 20000;
int stepSize = 50;
int[] blockEnds = ends(start, end, blockSize);
for (int j=1; j<blockEnds.length; ++j) {
int blockStart = blockEnds[j-1];
int blockEnd = blockEnds[j];
GTRec[] recBlock = stage2Haps.toGTRecs(blockStart, blockEnd);
int[] stepEnds = ends(blockStart, blockEnd, stepSize);
UnsignedByteArray[] output = IntStream.range(1, stepEnds.length)
.parallel()
.mapToObj(i -> toByteArray(samples, Arrays.copyOfRange(recBlock,
(stepEnds[i-1]-blockStart), (stepEnds[i]-blockStart))))
.toArray(UnsignedByteArray[]::new);
append(output, vcfOutFile);
}
long t1 = System.nanoTime();
System.out.println("WindWriter tot: " + Utilities.elapsedNanos(t1-t0));
}
private static void checkInterval(int start, int end, int nMarkers) {
if (start<0 || end>nMarkers || end<start) {
throw new IllegalArgumentException("start=" + start + " end=" + end
+ " nMarkers=" + nMarkers);
}
}
private static int[] ends(int start, int end, int step) {
IntList starts = new IntList(2 + ((end - start) / step));
for (int m=start; m<end; m+=step) {
starts.add(m);
}
starts.add(end);
return starts.toArray();
}
private static UnsignedByteArray toByteArray(GT phasedTarg, int start, int end) {
ByteArrayOutputStream baos = new ByteArrayOutputStream();
try (PrintWriter vcfOut=new PrintWriter(
new BGZIPOutputStream(baos, false))) {
VcfWriter.appendRecords(phasedTarg, start, end, vcfOut);
}
return new UnsignedByteArray(baos);
}
private static UnsignedByteArray toByteArray(Samples samples, GTRec[] phasedRecs) {
ByteArrayOutputStream baos = new ByteArrayOutputStream();
try (PrintWriter vcfOut=new PrintWriter(
new BGZIPOutputStream(baos, false))) {
VcfWriter.appendRecords(samples, phasedRecs, vcfOut);
}
return new UnsignedByteArray(baos);
}
private static void append(UnsignedByteArray[] output, File outFile) {
boolean append = true;
try {
try (OutputStream fos = new BufferedOutputStream(
new FileOutputStream(outFile, append))) {
for (UnsignedByteArray uba : output) {
uba.write(fos);
}
}
} catch (IOException e) {
fileOutputError(outFile, e);
}
}
@Override
public void close() {
boolean append = true;
try {
try (FileOutputStream fos = new FileOutputStream(vcfOutFile, append);
BufferedOutputStream bos = new BufferedOutputStream(fos);
BGZIPOutputStream bgzip = new BGZIPOutputStream(bos, true)) {
// write empty BGZIP block to bgzip by closing bgzip
}
} catch (IOException e) {
Utilities.exit(e, "Error closing file: " + vcfOutFile);
}
}
private static void fileOutputError(File file, Exception e) {
Utilities.exit(e, "Error writing to file: " + file);
}
}
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