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/*
* Copyright (C) 2014-2021 Brian L. Browning
*
* This file is part of Beagle
*
* Beagle is free software: you can redistribute it and/or modify
* it under the terms of the GNU General Public License as published by
* the Free Software Foundation, either version 3 of the License, or
* (at your option) any later version.
*
* Beagle is distributed in the hope that it will be useful,
* but WITHOUT ANY WARRANTY; without even the implied warranty of
* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
* GNU General Public License for more details.
*
* You should have received a copy of the GNU General Public License
* along with this program. If not, see <http://www.gnu.org/licenses/>.
*/
package vcf;
import blbutil.Const;
import blbutil.StringUtil;
import java.util.Arrays;
import java.util.Collections;
import java.util.HashMap;
import java.util.Map;
/**
* <p>Class {@code VcfRec} represents a VCF record. If one allele in a
* diploid genotype is missing, then both alleles are set to missing.
* </p>
* <p>Instances of class {@code VcfRec} are immutable.
* </p>
*
* @author Brian L. Browning {@code <browning@uw.edu>}
*/
public final class VcfRec implements GTRec {
private static final int SAMPLE_OFFSET = 9;
private final VcfHeader vcfHeader;
private final String vcfRecord;
private final int[] delimiters;
private final Marker marker;
private final String[] formatFields;
private final Map<String, Integer> formatMap;
private final GTRec gtRec;
/**
* Returns the VCF genotype index for the specified pair of alleles.
* @param a1 the first allele
* @param a2 the second allele
* @return the VCF genotype index for the specified pair of alleles
* @throws IllegalArgumentException if {@code a1 < 0 || a2 < 0}
*/
public static int gtIndex(int a1, int a2) {
if (a1 < 0) {
throw new IllegalArgumentException("a1<0: " + a1);
}
if (a2 < 0) {
throw new IllegalArgumentException("a2<0: " + a2);
} else if (a1 < a2) {
return (a2 * (a2 + 1)) / 2 + a1;
} else {
return (a1 * (a1 + 1)) / 2 + a2;
}
}
/**
* Constructs a new {@code VcfRec} instance from a VCF record.
*
* @param vcfHeader meta-information lines and header line for the
* specified VCF record.
* @param vcfRecord a VCF record with a GL format field corresponding to
* the specified {@code vcfHeader} object
* @param markerParser a filter for a VCF record's ID, QUAL, FILTER, and
* INFO subfields
*
* @throws IllegalArgumentException if the VCF record does not have a
* GT format field
* @throws IllegalArgumentException if a VCF record format error is
* detected
* @throws IllegalArgumentException if there are not
* {@code vcfHeader.nHeaderFields()} tab-delimited fields in the
* specified VCF record
* @throws NullPointerException if
* {@code (vcfHeader == null) || (vcfRecord == null) || (markerParser == null)}
*/
public VcfRec(VcfHeader vcfHeader, String vcfRecord,
MarkerParser markerParser) {
this.vcfHeader = vcfHeader;
this.vcfRecord = vcfRecord;
this.delimiters = delimiters(vcfHeader, vcfRecord);
this.marker = Marker.instance(vcfRecord, markerParser);
this.formatFields = formats(format());
this.formatMap = formatToIndexMap(vcfHeader, vcfRecord, formatFields);
if (formatMap.containsKey("GT")==false) {
String s = "Missing FORMAT/GT field: " + vcfRecord;
throw new IllegalArgumentException(s);
}
this.gtRec = new BasicGTRec(
new VcfRecGTParser(vcfHeader, vcfRecord, markerParser));
}
private static int[] delimiters(VcfHeader vcfHeader, String vcfRecord) {
int nFields = vcfHeader.nHeaderFields();
int[] delimiters = new int[nFields + 1];
delimiters[0] = -1;
for (int j=1; j<nFields; ++j) {
delimiters[j] = vcfRecord.indexOf(Const.tab, delimiters[j-1] + 1);
if (delimiters[j] == -1) {
fieldCountError(vcfHeader, vcfRecord);
}
}
if (vcfRecord.indexOf(Const.tab, delimiters[nFields-1] + 1) != -1) {
fieldCountError(vcfHeader, vcfRecord);
}
delimiters[nFields] = vcfRecord.length();
return delimiters;
}
private static void fieldCountError(VcfHeader vcfHeader, String vcfRecord) {
String src = "File source: " + vcfHeader.src();
String[] fields = StringUtil.getFields(vcfRecord, Const.tab);
String s = "VCF header line has " + vcfHeader.nHeaderFields()
+ " fields, but data line has " + fields.length + " fields"
+ Const.nl + "File source:" + src
+ Const.nl + Arrays.toString(fields);
throw new IllegalArgumentException(s);
}
private String[] formats(String formats) {
if (formats.equals(Const.MISSING_DATA_STRING) || formats.isEmpty()) {
String s = "missing format field: " + vcfRecord;
throw new IllegalArgumentException(s);
}
String[] fields = StringUtil.getFields(formats, Const.colon);
for (String f : fields) {
if (f.isEmpty()) {
String s = "missing format in format subfield list: " + vcfRecord;
throw new IllegalArgumentException(s);
}
}
return fields;
}
private static Map<String, Integer> formatToIndexMap(VcfHeader vcfHeader,
String vcfRecord, String[] formatFields) {
if (vcfHeader.nSamples()==0) {
return Collections.emptyMap();
}
Map<String, Integer> map = new HashMap<>(formatFields.length);
for (int j=0; j<formatFields.length; ++j) {
map.put(formatFields[j], j);
}
if (map.containsKey("GT") && map.get("GT")!=0) {
String s = "GT format is not first format: " + vcfRecord;
throw new IllegalArgumentException(s);
}
return map;
}
/* returns exclusive end */
private int formatSubfieldEnd(int start) {
while (start < vcfRecord.length()) {
char c = vcfRecord.charAt(start);
if (c == Const.colon || c == Const.tab) {
return start;
}
++start;
}
return start;
}
/**
* Returns the QUAL field.
* @return the QUAL field
*/
public String qual() {
return vcfRecord.substring(delimiters[5] + 1, delimiters[6]);
}
/**
* Returns the FILTER field.
* @return the FILTER field
*/
public String filter() {
return vcfRecord.substring(delimiters[6] + 1, delimiters[7]);
}
/**
* Returns the INFO field.
* @return the INFO field
*/
public String info() {
return vcfRecord.substring(delimiters[7] + 1, delimiters[8]);
}
/**
* Returns the FORMAT field. Returns the empty string ("") if the FORMAT
* field is missing.
* @return the FORMAT field
*/
public String format() {
if (delimiters.length > 9) {
return vcfRecord.substring(delimiters[8] + 1, delimiters[9]);
}
else {
return "";
}
}
/**
* Returns the number of FORMAT subfields.
* @return the number of FORMAT subfields
*/
public int nFormatSubfields() {
return formatFields.length;
}
/**
* Returns the specified FORMAT subfield.
* @param subfieldIndex a FORMAT subfield index
* @return the specified FORMAT subfield
*
* @throws IndexOutOfBoundsException if
* {@code subfieldIndex < 0 || subfieldIndex >= this.nFormatSubfields()}
*/
public String formatSubfield(int subfieldIndex) {
if (formatFields==null) {
throw new IllegalArgumentException("No format exists");
}
return formatFields[subfieldIndex];
}
/**
* Returns {@code true} if the specified FORMAT subfield is
* present, and returns {@code false} otherwise.
* @param formatCode a FORMAT subfield code
* @return {@code true} if the specified FORMAT subfield is
* present
*/
public boolean hasFormat(String formatCode) {
return formatMap.get(formatCode)!=null;
}
/**
* Returns the index of the specified FORMAT subfield if the
* specified subfield is defined for this VCF record, and returns -1
* otherwise.
* @param formatCode the format subfield code
* @return the index of the specified FORMAT subfield if the
* specified subfield is defined for this VCF record, and {@code -1}
* otherwise
*/
public int formatIndex(String formatCode) {
Integer index = formatMap.get(formatCode);
return (index==null) ? -1 : index;
}
/**
* Returns the data for the specified sample.
* @param sample a sample index
* @return the data for the specified sample
*
* @throws IndexOutOfBoundsException if
* {@code sample < 0 || sample >= this.size()}
*/
public String sampleData(int sample) {
int index = vcfHeader.unfilteredSampleIndex(sample);
return vcfRecord.substring(delimiters[index + SAMPLE_OFFSET] + 1,
delimiters[index + SAMPLE_OFFSET + 1]);
}
/**
* Returns the specified data for the specified sample.
* @param sample a sample index
* @param formatCode a FORMAT subfield code
* @return the specified data for the specified sample
*
* @throws IllegalArgumentException if
* {@code this.hasFormat(formatCode)==false}
* @throws IndexOutOfBoundsException if
* {@code sample < 0 || sample >= this.size()}
*/
public String sampleData(int sample, String formatCode) {
Integer formatIndex = formatMap.get(formatCode);
if (formatIndex==null) {
String s = "missing format data: " + formatCode;
throw new IllegalArgumentException(s);
}
return VcfRec.this.sampleData(sample, formatIndex);
}
/**
* Returns the specified data for the specified sample.
* @param sample a sample index
* @param subfieldIndex a FORMAT subfield index
* @return the specified data for the specified sample
*
* @throws IndexOutOfBoundsException if
* {@code field < 0 || field >= this.nFormatSubfields()}
* @throws IndexOutOfBoundsException if
* {@code sample < 0 || sample >= this.size()}
*/
public String sampleData(int sample, int subfieldIndex) {
if (subfieldIndex < 0 || subfieldIndex >= formatFields.length) {
throw new IndexOutOfBoundsException(String.valueOf(subfieldIndex));
}
int index = SAMPLE_OFFSET + vcfHeader.unfilteredSampleIndex(sample);
int start = delimiters[index] + 1;
for (int j = 0; j < subfieldIndex; ++j) {
int end = formatSubfieldEnd(start);
if (end==vcfRecord.length() || vcfRecord.charAt(end)==Const.tab) {
return ".";
}
else {
start = end + 1;
}
}
int end = formatSubfieldEnd(start);
if (end==start) {
return ".";
}
else {
return vcfRecord.substring(start, end);
}
}
/**
* Returns an array of length {@code this.size()}
* containing the specified FORMAT subfield data for each sample. The
* {@code k}-th element of the array is the specified FORMAT subfield data
* for the {@code k}-th sample.
* @param formatCode a format subfield code
* @return an array of length {@code this.size()}
* containing the specified FORMAT subfield data for each sample
*
* @throws IllegalArgumentException if
* {@code this.hasFormat(formatCode) == false}
*/
public String[] formatData(String formatCode) {
Integer formatIndex = formatMap.get(formatCode);
if (formatIndex==null) {
String s = "missing format data: " + formatCode;
throw new IllegalArgumentException(s);
}
String[] sa = new String[vcfHeader.nSamples()];
for (int j=0; j<sa.length; ++j) {
sa[j] = sampleData(j, formatIndex);
}
return sa;
}
@Override
public Samples samples() {
return vcfHeader.samples();
}
/**
* Returns the VCF meta-information lines and the VCF header line.
* @return the VCF meta-information lines and the VCF header line
*/
public VcfHeader vcfHeader() {
return vcfHeader;
}
@Override
public Marker marker() {
return marker;
}
@Override
public int get(int hap) {
return gtRec == null ? -1 : gtRec.get(hap);
}
@Override
public boolean isPhased(int sample) {
return gtRec == null ? false : gtRec.isPhased(sample);
}
@Override
public boolean isPhased() {
return gtRec == null ? false : gtRec.isPhased();
}
@Override
public int size() {
return 2*vcfHeader.nSamples();
}
/**
* Returns the VCF record.
* @return the VCF record
*/
@Override
public String toString() {
return vcfRecord;
}
}
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