1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300 301 302 303 304 305 306 307 308 309 310 311 312 313 314 315 316 317 318 319 320 321 322 323 324 325 326 327 328 329 330 331 332 333 334 335 336 337 338 339 340 341 342 343 344 345 346 347 348 349 350 351 352 353 354 355 356 357 358 359 360 361 362 363 364 365 366 367 368 369 370 371 372 373 374 375 376 377 378 379 380 381 382 383 384 385 386 387 388 389 390 391 392 393 394 395 396 397 398 399 400 401 402 403 404 405 406 407 408 409 410 411 412 413 414 415 416 417 418 419 420 421 422 423 424 425 426 427 428
|
/**
* BioJava development code
*
* This code may be freely distributed and modified under the
* terms of the GNU Lesser General Public Licence. This should
* be distributed with the code. If you do not have a copy,
* see:
*
* http://www.gnu.org/copyleft/lesser.html
*
* Copyright for this code is held jointly by the individual
* authors. These should be listed in @author doc comments.
*
* For more information on the BioJava project and its aims,
* or to join the biojava-l mailing list, visit the home page
* at:
*
* http://www.biojava.org/
*
*/
package org.biojava.bio.seq.io;
import java.io.BufferedReader;
import java.io.ByteArrayOutputStream;
import java.io.IOException;
import java.io.InputStream;
import java.io.InputStreamReader;
import junit.framework.Test;
import junit.framework.TestCase;
import junit.framework.TestSuite;
import org.biojava.bio.BioException;
import org.biojava.bio.seq.Sequence;
import org.biojava.bio.seq.SequenceIterator;
import org.biojava.bio.symbol.SymbolList;
/**
* JUnit test for SeqIOTools objects
* @author David Huen
* @since 1.3
*/
public class SeqIOToolsTest extends TestCase
{
public SeqIOToolsTest(String name)
{
super(name);
}
private boolean compareSymbolLists(SymbolList sl0, SymbolList sl1)
{
// compare lengths
if (sl0.length() != sl1.length()) return false;
// compare symbols
for (int i=1; i <= sl0.length(); i++) {
if (sl0.symbolAt(i) != sl1.symbolAt(i)) return false;
}
return true;
}
public void testDNAReadersAndWriters()
{
/******* test readFastaDNA *********/
// get access to the test file
InputStream inputS = this.getClass().getResourceAsStream("/files/AY069118.fa");
assertNotNull(inputS);
// get SequenceIterator
SequenceIterator seqI = SeqIOTools.readFastaDNA(
new BufferedReader(new InputStreamReader(inputS)));
// get sequence
assertTrue(seqI.hasNext());
Sequence fastaDNASeq = null;
try {
fastaDNASeq = seqI.nextSequence();
}
catch (BioException be) {}
assertNotNull(fastaDNASeq);
// is its length correct?
assertEquals("Fasta sequence AY069118.fa had incorrect length", fastaDNASeq.length(), 1502);
ByteArrayOutputStream baos = new ByteArrayOutputStream();
try {
SeqIOTools.writeFasta(baos, fastaDNASeq);
}
catch (IOException ex) {
fail(ex.getMessage());
}
/******* test readGenbank **********/
// get access to the test file
inputS = this.getClass().getResourceAsStream("/files/AY069118.gb");
assertNotNull(inputS);
// get SequenceIterator
seqI = SeqIOTools.readGenbank(
new BufferedReader(new InputStreamReader(inputS)));
// get sequence
assertTrue(seqI.hasNext());
Sequence genbankDNASeq = null;
try {
genbankDNASeq = seqI.nextSequence();
}
catch (BioException be) {}
assertNotNull(genbankDNASeq);
// is its length correct?
assertEquals("Genbank sequence AY069118.gb had incorrect length", genbankDNASeq.length(), 1502);
// compare with fasta reference
assertTrue(compareSymbolLists(fastaDNASeq, genbankDNASeq));
baos = new ByteArrayOutputStream();
try {
SeqIOTools.writeFasta(baos, genbankDNASeq);
}
catch (IOException ex) {
fail(ex.getMessage());
}
/******* test readGenbankXml ***********/
// get access to the test file
inputS = this.getClass().getResourceAsStream("/files/AY069118-gb.xml");
assertNotNull(inputS);
// get SequenceIterator
seqI = SeqIOTools.readGenbankXml(
new BufferedReader(new InputStreamReader(inputS)));
// get sequence
assertTrue(seqI.hasNext());
Sequence genbankXmlDNASeq = null;
try {
genbankXmlDNASeq = seqI.nextSequence();
}
catch (BioException be) {}
assertNotNull(genbankXmlDNASeq);
// is its length correct?
assertEquals("GenbankXml sequence AY069118-gb.xml had incorrect length", genbankXmlDNASeq.length(), 1502);
// compare with fasta reference
assertTrue(compareSymbolLists(fastaDNASeq, genbankXmlDNASeq));
baos = new ByteArrayOutputStream();
try {
SeqIOTools.writeFasta(baos, genbankXmlDNASeq);
}
catch (IOException ex) {
fail(ex.getMessage());
}
/******* test readEmblNucleotide **********/
// get access to the test file
inputS = this.getClass().getResourceAsStream("/files/AY069118.em");
assertNotNull(inputS);
// get SequenceIterator
seqI = SeqIOTools.readEmblNucleotide(
new BufferedReader(new InputStreamReader(inputS)));
// get sequence
assertTrue(seqI.hasNext());
Sequence emblDNASeq = null;
try {
emblDNASeq = seqI.nextSequence();
}
catch (BioException be) {}
assertNotNull(emblDNASeq);
// is its length correct?
assertEquals("Genbank sequence AY069118.em had incorrect length", emblDNASeq.length(), 1502);
// compare with fasta reference
assertTrue(compareSymbolLists(fastaDNASeq, emblDNASeq));
baos = new ByteArrayOutputStream();
try {
SeqIOTools.writeFasta(baos, emblDNASeq);
}
catch (IOException ex) {
fail(ex.getMessage());
}
}
public void testProteinReadersAndWriters()
{
/******* test readFastaProtein *********/
// get access to the test file
InputStream inputS = this.getClass().getResourceAsStream("/files/AAL039263.fa");
assertNotNull(inputS);
// get SequenceIterator
SequenceIterator seqI = SeqIOTools.readFastaProtein(
new BufferedReader(new InputStreamReader(inputS)));
// get sequence
assertTrue(seqI.hasNext());
Sequence fastaProteinSeq = null;
try {
fastaProteinSeq = seqI.nextSequence();
}
catch (BioException be) {}
assertNotNull(fastaProteinSeq);
// is its length correct?
assertEquals("Fasta sequence AAL39263.fa had incorrect length", fastaProteinSeq.length(), 370);
ByteArrayOutputStream baos = new ByteArrayOutputStream();
try {
SeqIOTools.writeFasta(baos, fastaProteinSeq);
}
catch (IOException ex) {
fail(ex.getMessage());
}
/******* test readGenpept **********/
// get access to the test file
inputS = this.getClass().getResourceAsStream("/files/AAL039263.gb");
assertNotNull(inputS);
// get SequenceIterator
seqI = SeqIOTools.readGenpept(
new BufferedReader(new InputStreamReader(inputS)));
// get sequence
assertTrue(seqI.hasNext());
Sequence genbankProteinSeq = null;
try {
genbankProteinSeq = seqI.nextSequence();
}
catch (BioException be) {}
assertNotNull(genbankProteinSeq);
// is its length correct?
assertEquals("Genbank sequence AAL39263.gb had incorrect length", genbankProteinSeq.length(), 370);
// compare with fasta reference
assertTrue(compareSymbolLists(fastaProteinSeq, genbankProteinSeq));
baos = new ByteArrayOutputStream();
try {
SeqIOTools.writeGenpept(baos, genbankProteinSeq);
}
catch (Exception ex) {
fail(ex.getMessage());
}
/******* test readSwissProt **********/
// get access to the test file
System.out.println("Testing SP read");
System.out.println("Testing SP read");
inputS = this.getClass().getResourceAsStream("/files/AAC4_HUMAN.sp");
assertNotNull(inputS);
// get SequenceIterator
seqI = SeqIOTools.readSwissprot(
new BufferedReader(new InputStreamReader(inputS)));
// get sequence
assertTrue(seqI.hasNext());
Sequence swissProteinSeq = null;
try {
swissProteinSeq = seqI.nextSequence();
}
catch (BioException be) {}
assertNotNull(swissProteinSeq);
// is its length correct?
assertEquals("SwissProt sequence AAC4_HUMAN.sp had incorrect length", swissProteinSeq.length(), 911);
baos = new ByteArrayOutputStream();
try {
SeqIOTools.writeSwissprot(baos, swissProteinSeq);
}
catch (Exception ex) {
fail(ex.getMessage());
}
}
public void testBigDNA()
{
/****** read big sequence as fasta ***********/
// get access to the test file
InputStream inputS = this.getClass().getResourceAsStream("/files/NC_004432.fa");
assertNotNull(inputS);
// get SequenceIterator
SequenceIterator seqI = SeqIOTools.readFastaDNA(
new BufferedReader(new InputStreamReader(inputS)));
// get sequence
assertTrue(seqI.hasNext());
Sequence bigFastaDNASeq = null;
try {
bigFastaDNASeq = seqI.nextSequence();
}
catch (BioException be) {}
assertNotNull(bigFastaDNASeq);
// is its length correct?
assertEquals("Fasta sequence NC_004432.fa had incorrect length", bigFastaDNASeq.length(), 1358633);
/****** read big sequence as genbank ***********/
// get access to the test file
inputS = this.getClass().getResourceAsStream("/files/NC_004432.gb");
assertNotNull(inputS);
// get SequenceIterator
seqI = SeqIOTools.readGenbank(
new BufferedReader(new InputStreamReader(inputS)));
// get sequence
assertTrue(seqI.hasNext());
Sequence bigGenbankDNASeq = null;
try {
bigGenbankDNASeq = seqI.nextSequence();
}
catch (BioException be) {}
assertNotNull(bigGenbankDNASeq);
// is its length correct?
assertEquals("Genbank sequence NC_004432.gb had incorrect length", bigGenbankDNASeq.length(), 1358633);
// compare with fasta reference
assertTrue(compareSymbolLists(bigFastaDNASeq, bigGenbankDNASeq));
}
public void testIdentifyFormat()
{
// Test formats which may be in any alphabet
String [] formats = new String [] { "raw", "fasta",
"nbrf", "ig",
"embl", "genbank",
"refseq", "gcg",
"gff",
"clustal", "msf" };
int [] formatIds = new int [] { SeqIOConstants.RAW, SeqIOConstants.FASTA,
SeqIOConstants.NBRF, SeqIOConstants.IG,
SeqIOConstants.EMBL, SeqIOConstants.GENBANK,
SeqIOConstants.REFSEQ, SeqIOConstants.GCG,
SeqIOConstants.GFF,
AlignIOConstants.CLUSTAL, AlignIOConstants.MSF };
String [] alphas = new String [] { "dna", "rna",
"aa", "protein" };
int [] alphaIds = new int [] { SeqIOConstants.DNA, SeqIOConstants.RNA,
SeqIOConstants.AA, SeqIOConstants.AA };
for (int i = 0; i < formats.length; i++)
{
for (int j = 0; j < alphas.length; j++)
{
assertEquals((formatIds[i] | alphaIds[j]),
SeqIOTools.identifyFormat(formats[i], alphas[j]));
}
}
// Test formats which throw exceptions unless in a specific
// alphabet
assertEquals(SeqIOConstants.SWISSPROT,
SeqIOTools.identifyFormat("swissprot", "protein"));
assertEquals(SeqIOConstants.SWISSPROT,
SeqIOTools.identifyFormat("swissprot", "aa"));
assertEquals(SeqIOConstants.SWISSPROT,
SeqIOTools.identifyFormat("swiss", "protein"));
assertEquals(SeqIOConstants.SWISSPROT,
SeqIOTools.identifyFormat("swiss", "aa"));
assertEquals(SeqIOConstants.GENPEPT,
SeqIOTools.identifyFormat("genpept", "protein"));
assertEquals(SeqIOConstants.GENPEPT,
SeqIOTools.identifyFormat("genpept", "aa"));
assertEquals(SeqIOConstants.PDB,
SeqIOTools.identifyFormat("pdb", "protein"));
assertEquals(SeqIOConstants.PDB,
SeqIOTools.identifyFormat("pdb", "aa"));
assertEquals(SeqIOConstants.PHRED,
SeqIOTools.identifyFormat("phred", "dna"));
}
// creates a suite
public static Test suite()
{
TestSuite suite = new TestSuite(SeqIOToolsTest.class);
return suite;
}
// harness for tests
public static void main(String [] args)
{
junit.textui.TestRunner.run(suite());
}
}
|