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# -*-Perl-*- Test Harness script for Bioperl
# $Id: CodonTable.t 15112 2008-12-08 18:12:38Z sendu $
use strict;
BEGIN {
use lib '.';
use Bio::Root::Test;
test_begin(-tests => 56);
use_ok('Bio::Tools::CodonTable');
use_ok('Bio::CodonUsage::IO');
}
# create a table object by giving an ID
my $DEBUG = test_debug();
my $myCodonTable = Bio::Tools::CodonTable -> new ( -id => 16);
ok defined $myCodonTable;
isa_ok $myCodonTable, 'Bio::Tools::CodonTable';
# defaults to ID 1 "Standard"
$myCodonTable = Bio::Tools::CodonTable->new();
is $myCodonTable->id(), 1;
# change codon table
$myCodonTable->id(10);
is $myCodonTable->id, 10;
is $myCodonTable->name(), 'Euplotid Nuclear';
# enumerate tables as object method
my $table = $myCodonTable->tables();
cmp_ok (keys %{$table}, '>=', 17); # currently 17 known tables
is $table->{11}, q{"Bacterial"};
# enumerate tables as class method
$table = Bio::Tools::CodonTable->tables;
cmp_ok (values %{$table}, '>=', 17); # currently 17 known tables
is $table->{23}, 'Thraustochytrium Mitochondrial';
# translate codons
$myCodonTable->id(1);
eval {
$myCodonTable->translate();
};
ok ($@ =~ /EX/) ;
is $myCodonTable->translate(''), '';
my @ii = qw(ACT acu ATN gt ytr sar);
my @res = qw(T T X V L Z );
my $test = 1;
for my $i (0..$#ii) {
if ($res[$i] ne $myCodonTable->translate($ii[$i]) ) {
$test = 0;
print $ii[$i], ": |", $res[$i], "| ne |", $myCodonTable->translate($ii[$i]), "|\n" if( $DEBUG);
last ;
}
}
ok ($test);
is $myCodonTable->translate('ag'), '';
is $myCodonTable->translate('jj'), '';
is $myCodonTable->translate('jjg'), 'X';
is $myCodonTable->translate('gt'), 'V';
is $myCodonTable->translate('g'), '';
# a more comprehensive test on ambiguous codes
my $seq = <<SEQ;
atgaaraayacmacracwackacyacsacvachacdacbacxagragyatmatwatyathcarcayc
cmccrccwcckccyccsccvcchccdccbccxcgmcgrcgwcgkcgycgscgvcghcgdcgbcgxctmctrct
wctkctyctsctvcthctdctbctxgargaygcmgcrgcwgckgcygcsgcvgchgcdgcbgcxggmggrggw
ggkggyggsggvgghggdggbggxgtmgtrgtwgtkgtygtsgtvgthgtdgtbgtxtartaytcmtcrtcwt
cktcytcstcvtchtcdtcbtcxtgyttrttytramgamggmgrracratrayytaytgytrsaasagsartaa;
SEQ
$seq =~ s/\s+//g;
@ii = grep { length == 3 } split /(.{3})/, $seq;
print join (' ', @ii), "\n" if( $DEBUG);
my $prot = <<PROT;
MKNTTTTTTTTTTTRSIIIIQHPPPPPPPPPPPRRRRRRRRRRRLLLLLLLLLLLEDAAAAAAAAAAAGGG
GGGGGGGGVVVVVVVVVVV*YSSSSSSSSSSSCLF*RRRBBBLLLZZZ*
PROT
$prot =~ s/\s//;
@res = split //, $prot;
print join (' ', @res), "\n" if( $DEBUG );
$test = 1;
for my $i (0..$#ii) {
if ($res[$i] ne $myCodonTable->translate($ii[$i]) ) {
$test = 0;
print $ii[$i], ": |", $res[$i], "| ne |",
$myCodonTable->translate($ii[$i]), "| @ $i\n" if( $DEBUG);
last ;
}
}
ok $test;
# reverse translate amino acids
is $myCodonTable->revtranslate('U'), 0;
is $myCodonTable->revtranslate('O'), 0;
is $myCodonTable->revtranslate('J'), 9;
is $myCodonTable->revtranslate('I'), 3;
@ii = qw(A l ACN Thr sER ter Glx);
@res = (
[qw(gct gcc gca gcg)],
[qw(ggc gga ggg act acc aca acg)],
[qw(tct tcc tca tcg agt agc)],
[qw(act acc aca acg)],
[qw(tct tcc tca tcg agt agc)],
[qw(taa tag tga)],
[qw(gaa gag caa cag)]
);
$test = 1;
TESTING: {
for my $i (0..$#ii) {
my @codonres = $myCodonTable->revtranslate($ii[$i]);
for my $j (0..$#codonres) {
if ($codonres[$j] ne $res[$i][$j]) {
$test = 0;
print $ii[$i], ': ', $codonres[$j], " ne ",
$res[$i][$j], "\n" if( $DEBUG);
last TESTING;
}
}
}
}
ok $test;
# boolean tests
$myCodonTable->id(1);
ok $myCodonTable->is_start_codon('ATG');
is $myCodonTable->is_start_codon('GGH'), 0;
ok $myCodonTable->is_start_codon('HTG');
is $myCodonTable->is_start_codon('CCC'), 0;
ok $myCodonTable->is_ter_codon('UAG');
ok $myCodonTable->is_ter_codon('TaG');
ok $myCodonTable->is_ter_codon('TaR');
ok $myCodonTable->is_ter_codon('tRa');
is $myCodonTable->is_ter_codon('ttA'), 0;
ok $myCodonTable->is_unknown_codon('jAG');
ok $myCodonTable->is_unknown_codon('jg');
is $myCodonTable->is_unknown_codon('UAG'), 0;
is $myCodonTable->translate_strict('ATG'), 'M';
#
# adding a custom codon table
#
my @custom_table =
( 'test1',
'FFLLSSSSYY**CC*WLLLL**PPHHQQR*RRIIIMT*TT*NKKSSRRV*VVAA*ADDEE*GGG'
);
ok my $custct = $myCodonTable->add_table(@custom_table);
is $custct, 24;
is $myCodonTable->translate('atgaaraayacmacracwacka'), 'MKNTTTT';
ok $myCodonTable->id($custct);
is $myCodonTable->translate('atgaaraayacmacracwacka'), 'MKXXTTT';
# test doing this via Bio::PrimarySeq object
use Bio::PrimarySeq;
ok $seq = Bio::PrimarySeq->new(-seq=>'atgaaraayacmacracwacka', -alphabet=>'dna');
is $seq->translate()->seq, 'MKNTTTT';
is $seq->translate(undef, undef, undef, undef, undef, undef, $myCodonTable)->seq, 'MKXXTTT';
# test gapped translated
ok $seq = Bio::PrimarySeq->new(-seq => 'atg---aar------aay',
-alphabet => 'dna');
is $seq->translate->seq, 'M-K--N';
ok $seq = Bio::PrimarySeq->new(-seq =>'ASDFGHKL');
is $myCodonTable->reverse_translate_all($seq), 'GCBWSNGAYTTYGGVCAYAARYTN';
ok $seq = Bio::PrimarySeq->new(-seq => 'ASXFHKL');
is $myCodonTable->reverse_translate_all($seq), 'GCBWSNNNNTTYCAYAARYTN';
#
# test reverse_translate_best(), requires a Bio::CodonUsage::Table object
#
ok $seq = Bio::PrimarySeq->new(-seq =>'ACDEFGHIKLMNPQRSTVWY');
ok my $io = Bio::CodonUsage::IO->new(-file => test_input_file('MmCT'));
ok my $cut = $io->next_data();
is $myCodonTable->reverse_translate_best($seq,$cut), 'GCCTGCGACGAGTTCGGCCACATCAAGCTGATGAACCCCCAGCGCTCCACCGTGTGGTAC';
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