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#
# BioPerl module for Bio::SearchIO::blast_pull
#
# Please direct questions and support issues to <bioperl-l@bioperl.org>
#
# Cared for by Sendu Bala <bix@sendu.me.uk>
#
# Copyright Sendu Bala
#
# You may distribute this module under the same terms as perl itself
# POD documentation - main docs before the code
=head1 NAME
Bio::SearchIO::blast_pull - A parser for BLAST output
=head1 SYNOPSIS
# do not use this class directly it is available through Bio::SearchIO
use Bio::SearchIO;
my $in = Bio::SearchIO->new(-format => 'blast_pull',
-file => 't/data/new_blastn.txt');
while (my $result = $in->next_result) {
# this is a Bio::Search::Result::BlastPullResult object
print "Results for ", $result->query_name(), "\n";
while (my $hit = $result->next_hit) {
print $hit->name(), "\n";
while (my $hsp = $hit->next_hsp) {
print "length is ", $hsp->length(), "\n";
}
}
}
=head1 DESCRIPTION
This object implements a pull-parser for BLAST output. It is fast since it
only does work on request (hence 'pull').
Currently only NCBI BLASTN and BLASTP are supported.
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to
the Bioperl mailing list. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
=head2 Support
Please direct usage questions or support issues to the mailing list:
I<bioperl-l@bioperl.org>
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
of the bugs and their resolution. Bug reports can be submitted via the
web:
https://github.com/bioperl/bioperl-live/issues
=head1 AUTHOR - Sendu Bala
Email bix@sendu.me.uk
=head1 APPENDIX
The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _
=cut
# Let the code begin...
package Bio::SearchIO::blast_pull;
use strict;
use Bio::Search::Result::BlastPullResult;
use base qw(Bio::SearchIO Bio::PullParserI);
=head2 new
Title : new
Usage : my $obj = Bio::SearchIO::blast_pull->new();
Function: Builds a new Bio::SearchIO::blast_pull object
Returns : Bio::SearchIO::blast_pull
Args : -fh/-file => BLAST output filename
-format => 'blast_pull'
-evalue => float or scientific notation number to be used
as an evalue cutoff for hits
-score => integer or scientific notation number to be used
as a score value cutoff for hits
-piped_behaviour => 'temp_file'|'memory'|'sequential_read'
-piped_behaviour defines what the parser should do if the input is
an unseekable filehandle (eg. piped input), see
Bio::PullParserI::chunk for details. Default is 'memory'.
=cut
sub _initialize {
my ($self, @args) = @_;
# don't do normal SearchIO initialization
my ($writer, $file, $fh, $piped_behaviour, $evalue, $score) =
$self->_rearrange([qw(WRITER
FILE FH
PIPED_BEHAVIOUR
EVALUE
SCORE)], @args);
$self->writer($writer) if $writer;
$self->_fields( { ( header => undef,
algorithm => undef,
algorithm_version => undef,
algorithm_reference => '',
database_name => undef,
database_letters => undef,
database_entries => undef,
next_result => undef,
evalue_cutoff => '[unset]',
score_cutoff => '[unset]' ) } );
$self->_fields->{evalue_cutoff} = $evalue if $evalue;
$self->_fields->{score_cutoff} = $score if $score;
$self->_dependencies( { ( algorithm => 'header',
algorithm_version => 'header',
database_name => 'header',
database_letters => 'header',
database_entries => 'header' ) } );
$self->chunk($file || $fh || $self->throw("-file or -fh must be supplied"),
-piped_behaviour => $piped_behaviour || 'memory');
}
sub _discover_header {
my $self = shift;
$self->_chunk_seek(0);
my $header = $self->_get_chunk_by_end("\nQuery=");
$self->{_after_header} = $self->_chunk_tell;
#*** won't catch all types? only support blastn/p now anyway
$header =~ /^(\S+) (\S+\s+\S+)/;
$self->_fields->{algorithm} = $1;
$self->_fields->{algorithm_version} = $2;
my ($database) = $header =~ /^Database: (.+)/sm;
unless ($database) {
# earlier versions put query before database?
my $header2 = $self->_get_chunk_by_end(".done\n");
($database) = $header2 =~ /^Database: (.+)/sm;
}
$database =~ s/\s+(\d\S+) sequences; (\d\S+) total letters.*//s;
my $entries = $1;
my $letters = $2;
$database =~ s/\n//g;
$entries =~ s/,//g;
$letters =~ s/,//g;
$self->_fields->{database_name} = $database;
$self->_fields->{database_entries} = $entries;
$self->_fields->{database_letters} = $letters;
$self->_fields->{header} = 1;
}
sub _discover_next_result {
my $self = shift;
return if $self->{_after_results};
my $type = $self->get_field('algorithm'); # also sets _after_header if not set
if ($type eq 'BLASTN' || $type eq 'BLASTP') {
unless ($self->_sequential) {
$self->_chunk_seek($self->{_end_of_previous_result} || $self->{_after_header});
my ($start, $end) = $self->_find_chunk_by_end("\nQuery=");
return if ($start == $end);
unless ($end) {
$start = $self->{_end_of_previous_result} || $self->{_after_header};
$end = undef;
}
$self->_fields->{next_result} = Bio::Search::Result::BlastPullResult->new(-chunk => [($self->chunk, $start, $end)],
-parent => $self);
$self->{_end_of_previous_result} = $end;
}
else {
#*** doesn't work for the last result, needs fixing - try getting the database end chunk on failure?...
$self->throw("sequential mode not yet implemented");
my $chunk = $self->_get_chunk_by_end("\nQuery=");
$chunk || return;
$self->_fields->{next_result} = Bio::Search::Result::BlastPullResult->new(-chunk => [$chunk],
-parent => $self);
}
}
else {
$self->throw("Can only handle NCBI BLASTN and BLASTP right now");
}
}
=head2 next_result
Title : next_result
Usage : my $hit = $searchio->next_result;
Function: Returns the next Result from a search
Returns : Bio::Search::Result::ResultI object
Args : none
=cut
sub next_result {
my $self = shift;
my $result = $self->get_field('next_result') || return;
undef $self->_fields->{next_result};
$self->{'_result_count'}++;
return $result;
}
=head2 result_count
Title : result_count
Usage : my $count = $searchio->result_count
Function: Returns the number of results we have processed.
Returns : integer
Args : none
=cut
sub result_count {
my $self = shift;
return $self->{'_result_count'};
}
=head2 rewind
Title : rewind
Usage : $searchio->rewind;
Function: Allow one to reset the Result iterator to the beginning, so that
next_result() will subsequently return the first result and so on.
NB: result objects are not cached, so you will get new result objects
each time you rewind. Also, note that result_count() counts the
number of times you have called next_result(), so will not be able
tell you how many results there were in the file if you use rewind().
Returns : n/a
Args : none
=cut
sub rewind {
my $self = shift;
delete $self->{_end_of_previous_result};
}
1;
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