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#------------------------------------------------------------------
#
# BioPerl module Bio::Restriction::Enzyme::MultiCut
#
# Please direct questions and support issues to <bioperl-l@bioperl.org>
#
# Cared for by Heikki Lehvaslaiho, heikki-at-bioperl-dot-org
#
# You may distribute this module under the same terms as perl itself
#------------------------------------------------------------------
## POD Documentation:
=head1 NAME
Bio::Restriction::Enzyme::MultiCut - A single restriction endonuclease
=head1 SYNOPSIS
# set up a single restriction enzyme. This contains lots of
# information about the enzyme that is generally parsed from a
# rebase file and can then be read back
use Bio::Restriction::Enzyme;
=head1 DESCRIPTION
This module defines a restriction endonuclease class where one object
represents one of the distinct recognition sites for that enzyme. The
method L<others|others> stores references to other objects with
alternative sites.
In this schema each object within an EnzymeCollection can be checked
for matching a sequence.
REBASE report notation C<Bsp24I (8/13)GACNNNNNNTGG(12/7)> means:
Bsp24I
5' ^NNNNNNNNGACNNNNNNTGGNNNNNNNNNNNN^ 3'
3' ^NNNNNNNNNNNNNCTGNNNNNNACCNNNNNNN^ 5'
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
=head2 Support
Please direct usage questions or support issues to the mailing list:
I<bioperl-l@bioperl.org>
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via the
web:
https://github.com/bioperl/bioperl-live/issues
=head1 AUTHOR
Heikki Lehvaslaiho, heikki-at-bioperl-dot-org
=head1 CONTRIBUTORS
Rob Edwards, redwards@utmem.edu
=head1 COPYRIGHT
Copyright (c) 2003 Rob Edwards.
Some of this work is Copyright (c) 1997-2002 Steve A. Chervitz. All
Rights Reserved. This module is free software; you can redistribute
it and/or modify it under the same terms as Perl itself.
=head1 SEE ALSO
L<Bio::Restriction::Enzyme>, L<Bio::Restriction::Analysis>,
L<Bio::Restriction::EnzymeCollection>
=head1 APPENDIX
Methods beginning with a leading underscore are considered private and
are intended for internal use by this module. They are not considered
part of the public interface and are described here for documentation
purposes only.
=cut
package Bio::Restriction::Enzyme::MultiCut;
use strict;
use Data::Dumper;
use vars qw ();
use base qw(Bio::Restriction::Enzyme);
=head2 new
Title : new
Function
Function : Initializes the enzyme object
Returns : The Restriction::Enzyme::MultiCut object
Argument :
=cut
sub new {
my($class, @args) = @_;
my $self = $class->SUPER::new(@args);
my ($others) =
$self->_rearrange([qw(
OTHERS
)], @args);
$others && $self->others($others);
return $self;
}
=head2 others
Title : others
Usage : $re->others(@enz_obj_array);
Function : Stores auxiliary Enzyme::MultiCut objects for multicutting enzymes
Arguments : optional array of Enzyme::MultiCut objects
Returns : array of Enzyme objects
Added for compatibility to REBASE
=cut
sub others {
my $self = shift;
push @{$self->{_others}}, @_ if @_;
return unless $self->{_others};
return @{$self->{'_others'}};
}
=head2 purge_others
Title : purge_others
Usage : $re->purge_references();
Function : Purges the set of references for this enzyme
Arguments :
Returns :
=cut
sub purge_others {
my ($self) = shift;
$self->{_others} = [];
}
1;
|
