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BLASTP 2.1.3 [Apr-11-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= gi|1786183|gb|AAC73113.1| (AE000111) aspartokinase I,
homoserine dehydrogenase I [Escherichia coli]
(820 letters)
Database: ecoli.aa
4289 sequences; 1,358,990 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAC73113.1| (AE000111) aspartokinase I, homoserine dehydrogen... 1567 0.0
gb|AAC76922.1| (AE000468) aspartokinase II and homoserine dehydr... 332 1e-91
gb|AAC76994.1| (AE000475) aspartokinase III, lysine sensitive [E... 184 3e-47
gb|AAC73282.1| (AE000126) uridylate kinase [Escherichia coli] 42 3e-04
>gb|AAC73113.1| (AE000111) aspartokinase I, homoserine dehydrogenase I [Escherichia
coli]
Length = 820
Score = 1567 bits (4058), Expect = 0.0
Identities = 806/820 (98%), Positives = 806/820 (98%)
Query: 1 MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQDA 60
MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQDA
Sbjct: 1 MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQDA 60
Query: 61 LPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCPDSINA 120
LPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCPDSINA
Sbjct: 61 LPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCPDSINA 120
Query: 121 ALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIAESTRRIAASRIP 180
ALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIAESTRRIAASRIP
Sbjct: 121 ALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIAESTRRIAASRIP 180
Query: 181 ADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQV 240
ADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQV
Sbjct: 181 ADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQV 240
Query: 241 PDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGTLIGASRD 300
PDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGTLIGASRD
Sbjct: 241 PDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGTLIGASRD 300
Query: 301 EDELPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLITQSSSEYSISF 360
EDELPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLITQSSSEYSISF
Sbjct: 301 EDELPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLITQSSSEYSISF 360
Query: 361 CVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTLRGISAKFFAAL 420
CVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTLRGISAKFFAAL
Sbjct: 361 CVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTLRGISAKFFAAL 420
Query: 421 ARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLFNTDQXXXXXXXXXXXXXXAL 480
ARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLFNTDQ AL
Sbjct: 421 ARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLFNTDQVIEVFVIGVGGVGGAL 480
Query: 481 LEQLKRQQSWLKNKHIDLRVCGVANSKALLTNVHGLNLENWQEELAQAKEPFNLGRLIRL 540
LEQLKRQQSWLKNKHIDLRVCGVANSKALLTNVHGLNLENWQEELAQAKEPFNLGRLIRL
Sbjct: 481 LEQLKRQQSWLKNKHIDLRVCGVANSKALLTNVHGLNLENWQEELAQAKEPFNLGRLIRL 540
Query: 541 VKEYHLLNPVIVDCTSSQAVADQYADFLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSR 600
VKEYHLLNPVIVDCTSSQAVADQYADFLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSR
Sbjct: 541 VKEYHLLNPVIVDCTSSQAVADQYADFLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSR 600
Query: 601 RKFLYDTNVGAGLPVIENLQNLLNAGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLA 660
RKFLYDTNVGAGLPVIENLQNLLNAGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLA
Sbjct: 601 RKFLYDTNVGAGLPVIENLQNLLNAGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLA 660
Query: 661 REMGYTEPDPRDDLSGMDVARKLLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMA 720
REMGYTEPDPRDDLSGMDVARKLLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMA
Sbjct: 661 REMGYTEPDPRDDLSGMDVARKLLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMA 720
Query: 721 NLSQLDDLFAARVAKARDEGKVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAF 780
NLSQLDDLFAARVAKARDEGKVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAF
Sbjct: 721 NLSQLDDLFAARVAKARDEGKVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAF 780
Query: 781 YSHYYQPLPLVLRGYGAGNDVTAAGVFADLLRTLSWKLGV 820
YSHYYQPLPLVLRGYGAGNDVTAAGVFADLLRTLSWKLGV
Sbjct: 781 YSHYYQPLPLVLRGYGAGNDVTAAGVFADLLRTLSWKLGV 820
>gb|AAC76922.1| (AE000468) aspartokinase II and homoserine dehydrogenase II
[Escherichia coli]
Length = 810
Score = 332 bits (850), Expect = 1e-91
Identities = 243/821 (29%), Positives = 403/821 (48%), Gaps = 44/821 (5%)
Query: 5 KFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQDALPNI 64
KFGG+S+A+ + +LRVA I+ ++ + V+SA TN L+ ++ + + + + +
Sbjct: 16 KFGGSSLADVKCYLRVAGIMAEYSQPDDMM-VVSAAGSTTNQLINWLKLSQTDRLSAHQV 74
Query: 65 SDAERIF-AELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCPDSINAALI 123
R + +L++GL A+ L + FV + GI+ D++ A ++
Sbjct: 75 QQTLRRYQCDLISGLLPAEEADSL--ISAFVSDLERLAALLDSGIN------DAVYAEVV 126
Query: 124 CRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIAESTRRIAASRIPADH 183
GE S +M+ VL +G +D E L A + + E ++ H
Sbjct: 127 GHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAER---AAQPQVDEGLSYPLLQQLLVQH 183
Query: 184 ---MVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQV 240
+++ GF + N GE V+LGRNGSDYSA + A IW+DV GVY+ DPR+V
Sbjct: 184 PGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVYSADPRKV 243
Query: 241 PDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGTLIGASRD 300
DA LL + EA EL+ A VLH RT+ P++ +I ++ + P G++R
Sbjct: 244 KDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSEIDLQLRCSYTPDQ-----GSTRI 298
Query: 301 EDELP----VKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLITQSSSEY 356
E L + +++ +++ + P + + + RA++ + + +
Sbjct: 299 ERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAHKEIDQILKRAQVRPLAVGVHNDRQ 358
Query: 357 SISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTLRGISAKF 416
+ FC A + + E GL L + + LA++++VG G+ +F
Sbjct: 359 LLQFCYTSEVADSALKILDEA-------GLPGELRLRQGLALVAMVGAGVTRNPLHCHRF 411
Query: 417 FAALARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLFNTDQXXXXXXXXXXXX 476
+ L + Q S+ V+ + ++ HQ +F ++
Sbjct: 412 WQQLKGQPVEFTW--QSDDGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLVLFGKGNI 469
Query: 477 XXALLEQLKRQQSWLKNKH-IDLRVCGVANSKALLTNVHGLN----LENWQEELAQAKEP 531
LE R+QS L + + + GV +S+ L + GL+ L + +E + E
Sbjct: 470 GSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYDGLDASRALAFFNDEAVEQDEE 529
Query: 532 FNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYADFLREGFHVVTPNKKANTSSMDYYHQ 591
L ++ + + V++D T+SQ +ADQY DF GFHV++ NK A S + Y Q
Sbjct: 530 ----SLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGFHVISANKLAGASDSNKYRQ 585
Query: 592 LRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLNAGDELMKFSGILSGSLSYIFGKLDEGM 651
+ A EK+ R +LY+ VGAGLP+ +++L+++GD ++ SGI SG+LS++F + D +
Sbjct: 586 IHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTILSISGIFSGTLSWLFLQFDGSV 645
Query: 652 SFSEATTLAREMGYTEPDPRDDLSGMDVARKLLILARETGRELELADIEIEPVLPAEFNA 711
F+E A + G TEPDPRDDLSG DV RKL+ILARE G +E + +E ++PA
Sbjct: 646 PFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAREAGYNIEPDQVRVESLVPAHCEG 705
Query: 712 EGDVAAFMANLSQLDDLFAARVAKARDEGKVLRYVGNIDEDGVCRVKIAEVDGNDPLFKV 771
G + F N +L++ R+ AR+ G VLRYV D +G RV + V + PL +
Sbjct: 706 -GSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVARFDANGKARVGVEAVREDHPLASL 764
Query: 772 KNGENALAFYSHYYQPLPLVLRGYGAGNDVTAAGVFADLLR 812
+N A S +Y+ PLV+RG GAG DVTA + +D+ R
Sbjct: 765 LPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGAIQSDINR 805
>gb|AAC76994.1| (AE000475) aspartokinase III, lysine sensitive [Escherichia coli]
Length = 449
Score = 184 bits (467), Expect = 3e-47
Identities = 142/471 (30%), Positives = 228/471 (48%), Gaps = 41/471 (8%)
Query: 3 VLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQ---- 58
V KFGGTSVA+ + R ADI+ S+A V VLSA A ITN LVA+ E G+
Sbjct: 6 VSKFGGTSVADFDAMNRSADIVLSDANVRLV--VLSASAGITNLLVALAEGLEPGERFEK 63
Query: 59 -DALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCP-- 115
DA+ NI F + + + + +I+ +L I++L +
Sbjct: 64 LDAIRNIQ-------------------FAILERLRYPNVIREEIERLLENITVLAEAAAL 104
Query: 116 ---DSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIAESTR 172
++ L+ GE MS + +L R D + + + + DIA
Sbjct: 105 ATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDIAALAE 164
Query: 173 RIAASRIPA--DHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVD 230
A +P + +V+ GF KG LGR GSDY+AA+LA L A +IWTDV
Sbjct: 165 LAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVDIWTDVP 224
Query: 231 GVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQA 290
G+YT DPR V A+ + +++ EA E++ FGAKVLHP T+ P + IP + ++ +P+A
Sbjct: 225 GIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFVGSSKDPRA 284
Query: 291 PGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLIT 350
GTL+ ++ E+ + ++ N + ++ M G A VF ++R ISV LIT
Sbjct: 285 GGTLV-CNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILARHNISVDLIT 343
Query: 351 QSSSEYSISFCVPQSDCV-RAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTL 409
+SE S++ + + + + + +EL L + V E LA+++++G+ +
Sbjct: 344 --TSEVSVALTLDTTGSTSTGDTLLTQSLLMEL--SALCRVEVEEGLALVALIGNDLSKA 399
Query: 410 RGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLF 460
G+ + F L NI + I G+S ++ +V +DA V+ H LF
Sbjct: 400 CGVGKEVFGVLEPFNIRM--ICYGASSHNLCFLVPGEDAEQVVQKLHSNLF 448
>gb|AAC73282.1| (AE000126) uridylate kinase [Escherichia coli]
Length = 241
Score = 41.6 bits (96), Expect = 3e-04
Identities = 28/97 (28%), Positives = 44/97 (44%), Gaps = 8/97 (8%)
Query: 199 LVVLGRNGSDYSAAVLAACLR-----ADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQE 253
+++ G+ + AACLR AD T VDGV+T DP + P A + + ++Y E
Sbjct: 132 VILSAGTGNPFFTTDSAACLRGIEIEADVVLKATKVDGVFTADPAKDPTATMYEQLTYSE 191
Query: 254 AMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQA 290
+E KV+ T ++P + N P A
Sbjct: 192 VLEKE---LKVMDLAAFTLARDHKLPIRVFNMNKPGA 225
Database: ecoli.aa
Posted date: Dec 6, 2001 1:58 PM
Number of letters in database: 1,358,990
Number of sequences in database: 4289
Lambda K H
0.319 0.135 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2022122
Number of Sequences: 4289
Number of extensions: 82424
Number of successful extensions: 256
Number of sequences better than 1.0e-03: 4
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 243
Number of HSP's gapped (non-prelim): 4
length of query: 820
length of database: 1,358,990
effective HSP length: 47
effective length of query: 773
effective length of database: 1,157,407
effective search space: 894675611
effective search space used: 894675611
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 92 (40.0 bits)
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