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# -*-Perl-*- Test Harness script for Bioperl
use strict;
BEGIN {
use Bio::Root::Test;
test_begin(-tests => 20);
use_ok('Bio::AlignIO::phylip');
}
my $DEBUG = test_debug();
my ($str,$aln,$strout,$status);
# PHYLIP sequential/non-interleaved
$strout = Bio::AlignIO->new('-file' => test_input_file('noninterleaved.phy'), '-interleaved' => 0,
'-format' => 'phylip');
$aln = $strout->next_aln($aln);
isa_ok($aln,'Bio::Align::AlignI');
is($aln->get_seq_by_pos(2)->seq(), 'CCTCAGATCACTCTTTGGCAACGACCCCTCGTCACAATAA'.
'AGGTAGGGGGGCAACTAAAGGAAGCTCTATTAGATACAGGAGCAGATGATACAGTATTAGAAGACATGAATT'.
'TGCCAGGAAGATGGAAACCAAAAATGATAGGGGGAATTGGAGGGTTTATCAAAGTAAGACAGTATGATCAGA'.
'TACCCATAGAGATCTGTGGACATAAAGCTATAGGTACAGTATTAGTAGGACCCACACCTGTCAATATAATTG'.
'GAAGAAATCTGTTGACTCAGATTGGTTGCACTTTAAATTTT' );
# PHYLIP interleaved with long Ids
$str = Bio::AlignIO->new(
'-file' => test_input_file("protpars_longid.phy"),
'-format' => 'phylip',
'longid' => 1);
$aln = $str->next_aln();
#isa_ok($str,'Bio::AlignIO');
isa_ok($aln,'Bio::Align::AlignI');
is $aln->get_seq_by_pos(1)->get_nse, 'S I N F R U P 0 0 1 /1-84';
is $aln->get_seq_by_pos(2)->get_nse, 'SINFRUP002/1-84';
# PHYLIP interleaved, multiple segments
$str = Bio::AlignIO->new(
'-file' => test_input_file("protpars.phy"),
'-format' => 'phylip');
$aln = $str->next_aln();
#isa_ok($str,'Bio::AlignIO');
isa_ok($aln,'Bio::Align::AlignI');
is $aln->get_seq_by_pos(1)->get_nse, 'SINFRUP001/1-4940';
# is $aln->get_seq_by_pos(2)->get_nse, 'SINFRUP002/1-84';
# PHYLIP interleaved
$str = Bio::AlignIO->new(
'-file' => test_input_file("testaln.phylip"),
'-format' => 'phylip');
$aln = $str->next_aln();
#isa_ok($str,'Bio::AlignIO');
isa_ok($aln,'Bio::Align::AlignI');
is $aln->get_seq_by_pos(1)->get_nse, 'Homo_sapie/1-45';
$strout = Bio::AlignIO->new(
'-file' => ">".test_output_file(),
'-format' => 'phylip');
$status = $strout->write_aln($aln);
is $status, 1, "phylip output test";
# check the LocatableSeq start/end/strand etc
my $ls = $aln->get_seq_by_pos(2);
is($ls->display_id, 'Pan_panisc');
is($ls->start, 1);
is($ls->end,47);
is($ls->length,50);
# bug 2984
TODO: {
local $TODO = 'problems with default strand';
# shouldn't this be 0?
is($ls->strand,0);
}
# check to see that newlines between header and sequences are parsed correctly
$str = Bio::AlignIO->new('-file' => test_input_file("codeml45b.mlc"), -format => 'phylip', '-longid' => 1);
$aln = $str->next_aln();
$ls = $aln->get_seq_by_pos(9);
ok($ls->display_id eq "Pop_trich_ch", "newline between header and sequences is parsed correctly");
# bug 123 "Problem with newer versions of AlignIO to write alignment data #123"
my $io = Bio::AlignIO->new(
-file => test_input_file("ORTHOMCL2345.cluster.aa.fa.aln.aa.phy.txt"),
-format => 'phylip');
$aln = $io->next_aln();
isa_ok($aln,'Bio::SimpleAlign');
# Alignment file where an id has spaces in it
$io = Bio::AlignIO->new(
-file => test_input_file("ids-with-spaces.phy"),
-format => 'phylip');
$aln = $io->next_aln();
isa_ok($aln,'Bio::SimpleAlign');
is($aln->get_seq_by_pos(1)->display_id, 'A_BAD_ID');
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