File: SeqIO.t

package info (click to toggle)
bioperl 1.7.8-1
  • links: PTS, VCS
  • area: main
  • in suites: bookworm, sid, trixie
  • size: 35,788 kB
  • sloc: perl: 94,019; xml: 14,811; makefile: 20
file content (174 lines) | stat: -rw-r--r-- 5,310 bytes parent folder | download | duplicates (2)
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
# -*-Perl-*- Test Harness script for Bioperl
# $Id$

use strict;

BEGIN {     
    use Bio::Root::Test;
    
    test_begin(-tests => 58);
    
    use_ok 'Bio::SeqIO';
}

my $verbose = test_debug();

my @formats = qw(gcg fasta raw pir tab ace );
# The following files or formats are failing: swiss genbank interpro embl

for my $format (@formats) {
    print "======== $format ========\n" if $verbose;
    my $seq;
    my $str = Bio::SeqIO->new( -file   => test_input_file("test.$format"),
                               -format => $format                          );

    is $str->format(), $format;

    ok $seq = $str->next_seq();
    print "Sequence 1 of 2 from $format stream:\n", $seq->seq, "\n\n" if  $verbose;
    unless ($format eq 'raw') {
        is $seq->id, 'roa1_drome',"ID for format $format";
        is $seq->length, 358;
    }
    
    unless ($format eq 'gcg') { # GCG file can contain only one sequence
        ok $seq = $str->next_seq();
        print "Sequence 2 of 2 from $format stream:\n", $seq->seq, $seq->seq, "\n" if $verbose;
    }
    
    my $outfile = test_output_file();
    my $out = Bio::SeqIO->new( -file   => ">$outfile",
                               -format => $format      );
    ok $out->write_seq($seq);
    if ($format eq 'fasta') {
        my $id_type;
        ok($id_type = $out->preferred_id_type('accession.version'),
            'accession.version');
    }
    
    ok -s $outfile;
}



# from testformats.pl
SKIP: {
    test_skip(-tests => 6, -requires_modules => [qw(Algorithm::Diff
                                                    IO::ScalarArray
                                                    IO::String)]);
    use_ok 'Algorithm::Diff';
    eval "use Algorithm::Diff qw(diff LCS);";
    use_ok 'IO::ScalarArray';
    use_ok 'IO::String';
    
    my %files = ( 
             #'test.embl'      => 'embl',
             #'test.ace'       => 'ace',
              'test.fasta'     => 'fasta',
             #'test.game'      => 'game',
              'test.gcg'       => 'gcg',
             #'test.genbank'   => 'genbank',
              'test.raw'       => 'raw',
             #'test_badlf.gcg' => 'gcg'
              );
    
    while( my ($file, $type) = each %files ) {
        my $filename = test_input_file($file);
        print "processing file $filename\n" if $verbose;
        open my $FILE, '<', $filename or die "Could not read file '$filename': $!\n";
        my @datain = <$FILE>;
        close $FILE;

        my $in = IO::String->new( join('', @datain) );
        my $seqin = Bio::SeqIO->new( -fh     => $in,
                                     -format => $type );
        my $out = IO::String->new();
        my $seqout = Bio::SeqIO->new( -fh     => $out,
                                      -format => $type );
        my $seq;
        while( defined($seq = $seqin->next_seq) ) {
            $seqout->write_seq($seq);
        }
        $seqout->close();
        $seqin->close();
        my $strref = $out->string_ref;
        my @dataout = map { $_."\n"} split(/\n/, $$strref );
        my @diffs = &diff( \@datain, \@dataout);
        is @diffs, 0;
        
        if(@diffs && $verbose) {
            foreach my $d ( @diffs ) {
                foreach my $diff ( @$d ) {
                    chomp($diff->[2]);
                    print $diff->[0], $diff->[1], "\n>", $diff->[2], "\n";
                }
            }
            print "in is \n", join('', @datain), "\n";
            print "out is \n", join('',@dataout), "\n";
        }
    }
}

# simple tests specific to Bio::SeqIO interface (applicable to all SeqIO
# modules)

##############################################
# test format() and variant() in Bio::RootIO
##############################################

my $in = Bio::SeqIO->new(
   -file    => test_input_file('bug2901.fa'),
   -format  => "fasta",
);
is $in->format, 'fasta';
is $in->variant, undef;

$in = Bio::SeqIO->new(
   -file    => test_input_file('fastq', 'illumina_faked.fastq'),
   -format  => "fastq",
   -variant => 'illumina',
);
is $in->format, 'fastq';
is $in->variant, 'illumina';


######################################################
# test format detection from different inputs
######################################################

$in = Bio::SeqIO->new( -file => test_input_file('test.fastq') );
is $in->format, 'fastq';

open my $fh, '<', test_input_file('test.genbank') or die "Could not read file 'test.genbank': $!\n";
$in = Bio::SeqIO->new( -fh => $fh );
is $in->format, 'genbank';
close $fh;

my $string = ">seq\nACGATCG\n";
$in = Bio::SeqIO->new( -string => $string );
is $in->format, 'fasta';


############ EXCEPTION HANDLING ############

TODO: {
    local $TODO = 'file/fh-based tests should be in Bio::Root::IO, see issue #3204';
    throws_ok {
        Bio::SeqIO->new();
    } qr/No file, fh, or string argument provided/, 'Must pass a file or file handle';
}

throws_ok {
    Bio::SeqIO->new(-fh => undef);
} qr/fh argument provided, but with an undefined value/,
    'Must pass a file or file handle';

throws_ok {
    Bio::SeqIO->new(-file => undef);
} qr/file argument provided, but with an undefined value/,
    'Must pass a file or file handle';

throws_ok {
    Bio::SeqIO->new(-file => 'foo.bar');
} qr/Could not read file 'foo.bar':/,
    'Must pass a real file';