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BLASTP 2.2.23+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: subject.fa
1 sequences; 311 total letters
Query= query
Length=220
Score E
Sequences producing significant alignments: (Bits) Value
subject 162 4e-45
> subject
Length=311
Score = 162 bits (411), Expect = 4e-45, Method: Compositional matrix adjust.
Identities = 110/308 (35%), Positives = 145/308 (47%), Gaps = 94/308 (30%)
Query 5 NNTQISGFLLMGLSNKPELQLPIFGLFLSMYLITVFGNLLIILDISSDSHLHTPMYFF-- 62
N + IS F L G+S PE Q +FG+FL MYL+T+ GNLLIIL I SD HLHTPMYFF
Sbjct 3 NQSSISEFFLRGISAPPEQQQSLFGIFLCMYLVTLTGNLLIILAIGSDLHLHTPMYFFLA 62
Query ------------------------------------------------------------
Sbjct 63 NLSFVDMGLTSSTVTKMLVNIQTRHHTISYTGCLTQMYFFLMFGDLDSFFLAAMAYDRYV 122
Query 63 --------------------------LANLXV--QSLMLLQLSFCSEVEIPHFFCELHQM 94
L N+ + ++ +LSFC EI HFFC++ +
Sbjct 123 AICHPLCYSTVMRPQVCALMLALCWVLTNIVALTHTFLMARLSFCVTGEIAHFFCDITPV 182
Query 95 IQLACSDTFLNDTVIYV--STVLLACGPLTGILYSYSKIVSSICRISSAQGKYKAFSTCA 152
++L+CSDT +N+ +++V TVL+ P I+ SY IV +I R+ + G KAFSTC+
Sbjct 183 LKLSCSDTHINEMMVFVLGGTVLIV--PFLCIVTSYIHIVPAILRVRTRGGVGKAFSTCS 240
Query 153 SHLSVVSLFYCTVLGVYLSCAATQSSHGSAVASVMYTVVTPMLNPFIYSLRNKDIKEALI 212
SHL VV +FY T+ YL + S A+ MYT+VTPMLNPFIYSLRNKD+K AL
Sbjct 241 SHLCVVCVFYGTLFSAYLCPPSIASEEKDIAAAAMYTIVTPMLNPFIYSLRNKDMKGALK 300
Query 213 RFLRRVTI 220
R +I
Sbjct 301 RLFSHRSI 308
Score = 15.0 bits (27), Expect = 1.9, Method: Compositional matrix adjust.
Identities = 6/10 (60%), Positives = 8/10 (80%), Gaps = 0/10 (0%)
Query 125 LYSYSKIVSS 134
L+S+ IVSS
Sbjct 302 LFSHRSIVSS 311
Lambda K H
0.328 0.139 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 55770
Database: subject.fa
Posted date: Apr 26, 2010 2:49 PM
Number of letters in database: 311
Number of sequences in database: 1
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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