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BLASTN 2.0MP-WashU [22-Aug-2004] [linux24-i686-ILP32F64 2004-08-22T21:42:14]
Copyright (C) 1996-2004 Washington University, Saint Louis, Missouri USA.
All Rights Reserved.
Reference: Gish, W. (1996-2004) http://blast.wustl.edu
Notice: this program and its default parameter settings are optimized to find
nearly identical sequences rapidly. To identify weak protein similarities
encoded in nucleic acid, use BLASTX, TBLASTN or TBLASTX.
Query= e15_99na_plate_1as gi|21844367|gb|BQ704948.1|BQ704948 Bn01_02k05_A
Bn01_AAFC_ECORC_transgenic_Brassica_napus_overexpressing_BNCBF17_constituti
vely_frost_tolerant Brassica napus cDNA clone Bn01_02k05, mRNA sequence
(598 letters)
Database: ATH1_chr_all.5con
5 sequences; 119,186,497 total letters.
Searching....10....20....30....40....50....60....70....80....90....100% done
Smallest
Sum
High Probability
Sequences producing High-scoring Segment Pairs: Score P(N) N
CHR1v01212004 932 1.3e-79 3
CHR5v01212004 1035 6.9e-71 2
>CHR1v01212004
Length = 30,432,563
Plus Strand HSPs:
Score = 932 (145.9 bits), Expect = 1.3e-79, Sum P(3) = 1.3e-79, Group = 1
Identities = 230/282 (81%), Positives = 240/282 (85%), Strand = Plus / Plus
Links = (1)-3-2
Query: 23 AAAGGARATTAAGCACCGGSTGAAGCTTGGTGGCTTGTAGGCGATGAAGACTGATGCRCT 82
|| |||+||||| |||| |||||||||||||||||||||| ||||| ||||||||+||
Sbjct: 25052120 AAGGGAAATTAA---CCGG-TGAAGCTTGGTGGCTTGTAGGCAATGAA-ACTGATGCACT 25052174
Query: 83 GGACCTGRCGCGTTGTTGTCGAATCCAATGATTCTAATAAAGGCGKTAGGGKACTCCGTT 142
|||| ||+|| || |||||||||||| ||||| ||||| ||||| +| |||+||||| ||
Sbjct: 25052175 GGACTTGACGGGT-GTTGTCGAATCCGATGATCCTAATGAAGGCATTGGGGTACTCC-TT 25052232
Query: 143 CTTGSACTCTTGCACTTCCTTCAACACTTGAGARGRGTCGGKGCATCCGARCAAAGGAAG 202
||||+|||||| |||||||||||||||||||| +|+|||||+||| ||||+||| |||||
Sbjct: 25052233 CTTGCACTCTTCCACTTCCTTCAACACTTGAGCGGAGTCGGTGCAACCGAACAAGGGAAG 25052292
Query: 203 CTTCCACATTGTCCAATAACGGCCATCGTAGTATCCAGGTGTGCTTCCATGCTCACGGTA 262
||||||||||||||| || || ||||| |||||||| |||| | | || |||||||||||
Sbjct: 25052293 CTTCCACATTGTCCAGTACCGTCCATCATAGTATCCGGGTGAGTTACCGTGCTCACGGTA 25052352
Query: 263 CACAAATCCGTGCTC--CAACTCAAATTCAACACAAGGAATC 302
|||||||||||||| ||| || || ||| || | ||
Sbjct: 25052353 CACAAATCCGTGCTATACAAACCACATGTTACA-AACCACTC 25052393
Score = 580 (93.1 bits), Expect = 1.3e-79, Sum P(3) = 1.3e-79, Group = 1
Identities = 153/192 (79%), Positives = 158/192 (82%), Strand = Plus / Plus
Links = 1-3-(2)
Query: 410 ACCTTCATGCAGCTAACTCTTCCTCCGTTGCTTGCAATGGAAGTAATATCAGTGTT-GCT 468
|||| ||||||| |||||||||| |||||||||| ||||||||||| || |||| ||
Sbjct: 25052740 ACCTGCATGCAGTTAACTCTTCCGCCGTTGCTTGTGATGGAAGTAATGTCGTTGTTAGCC 25052799
Query: 469 TTTGCGGGTGASTGGGAATGCRGCGGATGACTTCAAG-CCGGTRAAKWGGAGC-ACCATG 526
|| ||||||| +|||||| ||+||||| ||||| ||| ||| |+|| +||||| |||||
Sbjct: 25052800 TT-GCGGGTGGCTGGGAAGGCAGCGGAGGACTT-AAGTCCGTTGAA-AGGAGCGACCATA 25052856
Query: 527 GTGGCTTGAGCCGGTGAGGTAGCCACAGCGGCGGGAGGAGAGCATAGRAGGWAGCCATTA 586
||||| |||||||| |||| | ||| || |||| || ||||||||| |||+|||||||
Sbjct: 25052857 GTGGCCTGAGCCGGAGAGGCAACCATAGTAGCGGAAG-AGAGCATAG-AGGAAGCCATTG 25052914
Query: 587 CTACTTCTTTTC 598
| ||||||| |
Sbjct: 25052915 TT-CTTCTTTAC 25052925
Score = 512 (82.9 bits), Expect = 1.3e-79, Sum P(3) = 1.3e-79, Group = 1
Identities = 118/139 (84%), Positives = 121/139 (87%), Strand = Plus / Plus
Links = 1-(3)-2
Query: 275 CTCCAACTCAAATTCAACACAAGGAATCCACTCTGTTGCGGAGAAGGTAGTCAACTTCTT 334
||||||||| |||||||||||||||||||||| ||||||||| ||||||||||||||| |
Sbjct: 25052501 CTCCAACTCGAATTCAACACAAGGAATCCACT-TGTTGCGGATAAGGTAGTCAACTTCCT 25052559
Query: 335 TGGCCAATTCAACGTCAGTRAGGTCAGGRAGATAAGAGAGAGTCTCAGHACTTCTTCTTT 394
| |||||||| || ||+||||||||+|| ||||||||||||||| |||||||||||
Sbjct: 25052560 TAGCCAATTCGGAATCGGTAAGGTCAGGAAGGTAAGAGAGAGTCTCA-AACTTCTTCTTT 25052618
Query: 395 SCGACTGGKGGGCACACCT 413
+| | || || |||||||
Sbjct: 25052619 CCAATCGGAGGCCACACCT 25052637
>CHR5v01212004
Length = 26,992,728
Plus Strand HSPs:
Score = 1035 (161.3 bits), Expect = 6.9e-71, Sum P(2) = 6.9e-71, Group = 1
Identities = 272/348 (78%), Positives = 281/348 (80%), Strand = Plus / Plus
Links = (1)-2
Query: 1 GATTATT-ATATGATTGTTTTAGAAAGGARATTAAGCACCGGSTGAAGCTTGGTGGCTTG 59
||| ||| ||| || ||||||||||| ||+||||||| ||| |||||||||| ||||||
Sbjct: 15394700 GATAATTCATAAGAATGTTTTAGAAAAGAAATTAAGCTTCGG-TGAAGCTTGGGGGCTTG 15394758
Query: 60 TAGGCGATGAAGACTGATGCRCTGGACCTGRCGCGTTGTTGTCGAATCCAATGATTCTAA 119
||||| ||||| ||||||||+ ||||| ||+|| || |||||||||||| ||||| ||||
Sbjct: 15394759 TAGGCAATGAA-ACTGATGCATTGGACTTGACGGGT-GTTGTCGAATCCGATGATCCTAA 15394816
Query: 120 TAAAGGCGKTAGGGKACTCCGTTCTTGSACTCTTGCACTTCCTTCAACACTTGAGARGRG 179
| |||||| |||+||||| ||||||+| |||| ||||||||||||||||||| +|+|
Sbjct: 15394817 TGAAGGCGCCCGGGTACTCC-TTCTTGCATTCTTCAACTTCCTTCAACACTTGAGCGGAG 15394875
Query: 180 TCGGKGCATCCGARCAAAGGAAGCTTCCACATTGTCCAATAACGGCCATCGTAGTATCCA 239
||||+||||||||+||| |||||||||||||||||||| || || ||||||||||||||
Sbjct: 15394876 TCGGTGCATCCGAACAATGGAAGCTTCCACATTGTCCAGTACCGTCCATCGTAGTATCCG 15394935
Query: 240 GGTGTGCTTCCATGCTCACGGTACACAAATCCGTGCTCCAA-CTCA-AATTCAACACAAG 297
|| ||| |||| ||||||||||||||||||||||||| || | || || ||| ||
Sbjct: 15394936 GGAGTGTTTCCGTGCTCACGGTACACAAATCCGTGCTATAAACACATAAGAAAAC-CATT 15394994
Query: 298 GAATCCACTCTGTTGC-GGAGAAGGTAGTCAACTTCTT-TGGCCAATT 343
||| || | || | |||| | | ||| | | | | | |||
Sbjct: 15394995 CAATACAAT-TGATATTGGAGGATATGCACAAGGTGATATAGTCTATT 15395041
Score = 739 (116.9 bits), Expect = 6.9e-71, Sum P(2) = 6.9e-71, Group = 1
Identities = 166/189 (87%), Positives = 171/189 (90%), Strand = Plus / Plus
Links = 1-(2)
Query: 410 ACCTTCATGCAGCTAACTCTTCCTCCGTTGCTTGCAATGGAAGTAATATCAGTGTTGCTT 469
||||||||||||||||||||||| ||||||||||| |||||||| || || ||||| |
Sbjct: 15395364 ACCTTCATGCAGCTAACTCTTCCCCCGTTGCTTGCGATGGAAGTGATGTCCTTGTTGGTC 15395423
Query: 470 TTGCGGGTGASTGGGAATGCRGCGGATGACTTCAAGCCGGTRAAKWGGAGC-ACCATGGT 528
||||||||||+ ||||||||+||||||||||||||||||||+||+ ||||| ||||||||
Sbjct: 15395424 TTGCGGGTGACCGGGAATGCAGCGGATGACTTCAAGCCGGTGAAT-GGAGCGACCATGGT 15395482
Query: 529 GGCTTGAGCCGGTGAGGTAGCCACAGCGGCGGGAGGAGAGCATAGRAGGWAGCCATTACT 588
||| |||||||| || ||| |||||||||||| |||||||||||| |||+||||||||||
Sbjct: 15395483 GGCCTGAGCCGGGGATGTAACCACAGCGGCGG-AGGAGAGCATAG-AGGAAGCCATTACT 15395540
Query: 589 ACTTCTTTT 597
||||||| |
Sbjct: 15395541 ACTTCTTGT 15395549
Parameters:
-i /home/jasons/pubbrassica/pub_brassica.4
-d /home/jasons/pubbrassica/ATH1_chr_all.5con
E=1e-5
topcomboE=10
wordmask=seg
hspsepsmax=1000
links
ctxfactor=2.00
Query ----- As Used ----- ----- Computed ----
Strand MatID Matrix name Lambda K H Lambda K H
+1 0 +5,-4 0.195 0.175 0.361 same same same
Q=10,R=10 0.104 0.0151 0.0600 n/a n/a n/a
-1 0 +5,-4 0.195 0.175 0.361 same same same
Q=10,R=10 0.104 0.0151 0.0600 n/a n/a n/a
Query
Strand MatID Length Eff.Length E S W T X E2 S2
+1 0 598 598 1.0e-05 299 11 n/a 72 0.023 78
134 0.025 117
-1 0 598 598 1.0e-05 299 11 n/a 72 0.023 78
134 0.025 117
Statistics:
Database: /home/jasons/pubbrassica/ATH1_chr_all.5con
Title: ATH1_chr_all.5con
Posted: 11:14:54 AM EST Sep 1, 2004
Created: 12:14:50 PM EETDT Sep 01, 2004
Format: XDF-1
# of letters in database: 119,186,497
# of sequences in database: 5
# of database sequences satisfying E: 2
No. of states in DFA: 233 (233 KB)
Total size of DFA: 254 KB (2059 KB)
Time to generate neighborhood: 0.00u 0.01s 0.01t Elapsed: 00:00:00
No. of threads or processors used: 2
Search cpu time: 0.58u 0.06s 0.64t Elapsed: 00:00:00
Total cpu time: 0.66u 0.29s 0.95t Elapsed: 00:00:01
Start: Wed Sep 1 13:19:06 2004 End: Wed Sep 1 13:19:07 2004
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