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#
# BioPerl module for Bio::Tools::EMBOSS::Palindrome
#
# Please direct questions and support issues to <bioperl-l@bioperl.org>
#
# Cared for by Jason Stajich <jason-at-bioperl-dot-org>
#
# Copyright Jason Stajich
#
# You may distribute this module under the same terms as perl itself
# POD documentation - main docs before the code
=head1 NAME
Bio::Tools::EMBOSS::Palindrome - parse EMBOSS palindrome output
=head1 SYNOPSIS
# a simple script to turn palindrome output into GFF3
use Bio::Tools::EMBOSS::Palindrome;
use Bio::Tools::GFF;
my $parser = Bio::Tools::EMBOSS::Palindrome->new(-file => $filename);
my $out = Bio::Tools::GFF->new(-gff_version => 3,
-file => ">$filename.gff");
while( my $seq = $parser->next_seq ) {
for my $feat ( $seq->get_SeqFeatures ) {
$out->write_feature($feat);
}
}
=head1 DESCRIPTION
This is a parser for the EMBOSS tool 'palindrome'. It will produce a
L<Bio::Seq> object for each sequence analyzed. The sequence will be
empty (but will be of the correct length) and will have attached to it
L<Bio::SeqFeature::FeaturePair> objects which wil
=head2 FUTURE WORK
It may be consolidated into another framework at a later time, but for
the time being it will stay a separate modules.
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to
the Bioperl mailing list. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
=head2 Support
Please direct usage questions or support issues to the mailing list:
I<bioperl-l@bioperl.org>
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
of the bugs and their resolution. Bug reports can be submitted via
email or the web:
https://github.com/bioperl/bioperl-live/issues
=head1 AUTHOR - Jason Stajich
Email jason-at-bioperl-dot-org
=head1 APPENDIX
The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _
=cut
# Let the code begin...
package Bio::Tools::EMBOSS::Palindrome;
$Bio::Tools::EMBOSS::Palindrome::VERSION = '1.7.8';
use vars qw($DEFAULT_SOURCETAG);
use strict;
use Bio::SeqFeature::FeaturePair;
use Bio::SeqFeature::Generic;
use base qw(Bio::Root::IO);
$DEFAULT_SOURCETAG = 'palindrome';
=head2 new
Title : new
Usage : my $obj = Bio::Tools::EMBOSS::Palindrome->new();
Function: Builds a new Bio::Tools::EMBOSS::Palindrome object
Returns : an instance of Bio::Tools::EMBOSS::Palindrome
Args : -file/-fh => a filename or filehandle for
initializing the parser
=cut
=head2 next_seq
Title : next_seq
Usage : my $seq = $parser->next_seq;
Function: Get the next feature set from the
Returns : L<Bio::SeqI> object
Args : none
=cut
sub next_seq {
my ($self) = @_;
my (%searching, $seq,$state);
my $source = $self->source_tag;
$state = 0;
while(defined($_ = $self->_readline)) {
if( /^\s+$/ ) {
next;
} elsif( /^Palindromes\s+of\s*:\s+(\S+)/o ) {
$state = 0;
if( $seq ) {
$self->_pushback($_);
return $seq;
}
$seq = Bio::Seq->new(-display_id => $1);
# now get ready to store for the next record
$searching{'-seq_id'} = $1;
} elsif( /^Sequence\s+length\s+is\s*:\s+(\d+)/o ) {
$seq->length($1);
$searching{'-tag'}->{'seqlength'} = $1;
} elsif( /^(Start|End)\s+at\s+position\s*:\s+(\d+)/ ) {
$searching{'-tag'}->{lc($1)} = $2;
} elsif( m/^(Maximum|Minimum)\s+length\s+of\s+Palindromes\s+
is\s*:\s+(\d+)/ox) {
$searching{'-tag'}->{lc($1).'_length'} = $2;
} elsif( /^(Maximum\s+gap)\s+between\s+elements\s+is\s*:\s+(\d+)/o ) {
$searching{'-tag'}->{lc($1)} = $2;
} elsif( m/^Number\s+of\s+mismatches\s+allowed\s+
in\s+Palindrome\s*:\s+(\d+)/ox ) {
$searching{'-tag'}->{'allowed_mismatches'} = $1;
} elsif( /^Palindromes:/o ) {
$state = 1;
} elsif( $state == 1 ) {
my $feature = Bio::SeqFeature::FeaturePair->new
(-primary_tag => 'similarity',
-source_tag => $source);
for(my $i = 0; $i < 3; $i++ ) {
if ($i != 1) {
if( /^(\d+)\s+(\S+)\s+(\d+)/o ) {
my ($start,$match,$end) = ($1,$2,$3);
my $type = $i == 0 ? 'feature1' : 'feature2';
($start,$end) = sort { $a <=> $b } ($start,$end);
$feature->$type(
Bio::SeqFeature::Generic->new
(%searching,
-start => $start,
-end => $end,
-strand => $i == 0 ? 1 : -1,
-primary_tag => 'similarity',
-source_tag => $source)
);
} else {
chomp;
warn("Out of sync, line did not match:'$_'\n");
}
}
$_ = $self->_readline;
}
$seq->add_SeqFeature($feature);
}
}
return $seq;
}
=head2 source_tag
Title : source_tag
Usage : $obj->source_tag($newval)
Function: Get/Set Source Tag ('palindrome') by default
Returns : value of source_tag (a scalar)
Args : on set, new value (a scalar or undef, optional)
=cut
sub source_tag{
my $self = shift;
return $self->{'source_tag'} = shift if @_;
return $self->{'source_tag'} || $DEFAULT_SOURCETAG;
}
1;
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