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#!/usr/bin/perl -w
##
# long_read.pl
#
# Basic tests to ensure that long reads are handled properly.
#
use strict;
use warnings;
my $bowtie = "./bowtie";
my $bowtie_d = $bowtie . " --debug";
my $bowtie_l = $bowtie . " --large-index --debug";
if(system("$bowtie --version") != 0) {
$bowtie = `which bowtie`;
chomp($bowtie);
if(system("$bowtie --version") != 0) {
die "Could not find bowtie in current directory or in PATH\n";
}
}
if(system("$bowtie_d --version") != 0) {
die "Could not find bowtie-debug in current directory or in PATH\n";
}
if(system("$bowtie_l --version") != 0) {
die "Could not find bowtie large index support build in current directory or in PATH\n";
}
if(! -f "e_coli_c.1.ebwt") {
print STDERR "Making colorspace e_coli index\n";
my $bowtie_build = "./bowtie-build";
if(system("$bowtie_build --version") != 0) {
print STDERR "Could not execute ./bowtie-build; looking in PATH...\n";
$bowtie_build = `which $bowtie_build`;
chomp($bowtie_build);
if(system("$bowtie_build --version") != 0) {
die "Could not find bowtie-build in current directory or in PATH\n";
}
}
system("$bowtie_build -C genomes/NC_008253.fna e_coli_c") && die;
} else {
print STDERR "Colorspace e_coli index already present...\n";
}
if(! -f "e_coli_c.1.ebwtl") {
print STDERR "Making large colorspace e_coli index\n";
my $bowtie_build = "./bowtie-build --large-index";
if(system("$bowtie_build --version") != 0) {
print STDERR "Could not execute ./bowtie-build; looking in PATH...\n";
$bowtie_build = `which $bowtie_build`;
chomp($bowtie_build);
if(system("$bowtie_build --version") != 0) {
die "Could not find bowtie-build in current directory or in PATH\n";
}
}
system("$bowtie_build --large-index -C genomes/NC_008253.fna e_coli_c") && die;
} else {
print STDERR "Colorspace e_coli index already present...\n";
}
my @reads = (
# 70:
"CCGCGCCCCTGGACTTTGTAGCCACCGAAAATATTCACTGACTGTGGGGTTAGGCCTAAATGACCACAAA:".
"01111:::=????====???6111177=::::=???7444=??????====??===??????:::=????",
# 140:
"CCGCGCCCCTGGACTTTGTAGCCACCGAAAATATTCACTGACTGTGGGGTTAGGCCTAAATGACCACAAA".
"CTGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCCACCAGCCACTCACCGCCTTCAATTTTGACCAT:".
"01111:::=????====???6111177=::::=???7444=??????====??===??????:::=????".
"??????????=====;96666/.......;=>>>?DDDDDDDDDDDDDDDDDDDDDBBBBDDDDDCCCDD",
# 210:
"CCGCGCCCCTGGACTTTGTAGCCACCGAAAATATTCACTGACTGTGGGGTTAGGCCTAAATGACCACAAA".
"CTGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCCACCAGCCACTCACCGCCTTCAATTTTGACCAT".
"GTTGGCTCCGGCACGCATTACCGTTGCGGCGTTTTCAAAGGCTTGTTCCGGCGTGGCATACGCCATAAAT:".
"01111:::=????====???6111177=::::=???7444=??????====??===??????:::=????".
"??????????=====;96666/.......;=>>>?DDDDDDDDDDDDDDDDDDDDDBBBBDDDDDCCCDD".
"DDDDDDDDC>>>CDDDDDD>>?DDDDDDDDDDDDDCC???CCCCCCDDDDDDDDDDDDDDDDDDDDDDDD",
# 280:
"CCGCGCCCCTGGACTTTGTAGCCACCGAAAATATTCACTGACTGTGGGGTTAGGCCTAAATGACCACAAA".
"CTGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCCACCAGCCACTCACCGCCTTCAATTTTGACCAT".
"GTTGGCTCCGGCACGCATTACCGTTGCGGCGTTTTCAAAGGCTTGTTCCGGCGTGGCATACGCCATAAAT".
"GGCAGGTCAGCCAGCAGAAGGCAGTTTGGCGCGCCGCGACGCACAGCGGCGGTGTGATAGGCGATATCGG:".
"01111:::=????====???6111177=::::=???7444=??????====??===??????:::=????".
"??????????=====;96666/.......;=>>>?DDDDDDDDDDDDDDDDDDDDDBBBBDDDDDCCCDD".
"DDDDDDDDC>>>CDDDDDD>>?DDDDDDDDDDDDDCC???CCCCCCDDDDDDDDDDDDDDDDDDDDDDDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD",
# 350:
"CCGCGCCCCTGGACTTTGTAGCCACCGAAAATATTCACTGACTGTGGGGTTAGGCCTAAATGACCACAAA".
"CTGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCCACCAGCCACTCACCGCCTTCAATTTTGACCAT".
"GTTGGCTCCGGCACGCATTACCGTTGCGGCGTTTTCAAAGGCTTGTTCCGGCGTGGCATACGCCATAAAT".
"GGCAGGTCAGCCAGCAGAAGGCAGTTTGGCGCGCCGCGACGCACAGCGGCGGTGTGATAGGCGATATCGG".
"CAACGGTAACCGGTAGCGTGGAGTCATGCCCCTGGACCGTCATGCCCAGTGAATCGCCTACCAGCATGAC:".
"01111:::=????====???6111177=::::=???7444=??????====??===??????:::=????".
"??????????=====;96666/.......;=>>>?DDDDDDDDDDDDDDDDDDDDDBBBBDDDDDCCCDD".
"DDDDDDDDC>>>CDDDDDD>>?DDDDDDDDDDDDDCC???CCCCCCDDDDDDDDDDDDDDDDDDDDDDDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDDDDDDDDDDDDDDDAAADDDDDDD;;;;DDDEFFFDEGGGGDDDDDDDDDCCCDDDDDD",
# 700:
"CCGCGCCCCTGGACTTTGTAGCCACCGAAAATATTCACTGACTGTGGGGTTAGGCCTAAATGACCACAAA".
"CTGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCCACCAGCCACTCACCGCCTTCAATTTTGACCAT".
"GTTGGCTCCGGCACGCATTACCGTTGCGGCGTTTTCAAAGGCTTGTTCCGGCGTGGCATACGCCATAAAT".
"GGCAGGTCAGCCAGCAGAAGGCAGTTTGGCGCGCCGCGACGCACAGCGGCGGTGTGATAGGCGATATCGG".
"CAACGGTAACCGGTAGCGTGGAGTCATGCCCCTGGACCGTCATGCCCAGTGAATCGCCTACCAGCATGAC".
"GTTAATCCCTTCATCGGCAAACAGTTTTGCGAAGCTATAGTCGTAAGCGGTGATGGTTGCGAAACGTTTT".
"TTGTCCTGCTTGCATTTCTGCAATGAAGCAATCGTGGTTGGTTTCATAGCGTATCCTGATAAATGATGTT".
"GTGCTGTCTCGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTATCGCACCGCCGTTGTCT".
"GGCGGTGCGATAGTGTTATCAGGGGTAGGTAACCTGAAAAGTGCTGGTGGCTTTAAAATCCCCCGGTTCT".
"ATAGCAATATTACCGTCTTGTTTTAATGTAGCCTGGAAATGAAGAGGTTGAGAGGTCCCACCGTTGCCAT:".
"01111:::=????====???6111177=::::=???7444=??????====??===??????:::=????".
"??????????=====;96666/.......;=>>>?DDDDDDDDDDDDDDDDDDDDDBBBBDDDDDCCCDD".
"DDDDDDDDC>>>CDDDDDD>>?DDDDDDDDDDDDDCC???CCCCCCDDDDDDDDDDDDDDDDDDDDDDDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD",
# 980:
"CCGCGCCCCTGGACTTTGTAGCCACCGAAAATATTCACTGACTGTGGGGTTAGGCCTAAATGACCACAAA".
"CTGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCCACCAGCCACTCACCGCCTTCAATTTTGACCAT".
"GTTGGCTCCGGCACGCATTACCGTTGCGGCGTTTTCAAAGGCTTGTTCCGGCGTGGCATACGCCATAAAT".
"GGCAGGTCAGCCAGCAGAAGGCAGTTTGGCGCGCCGCGACGCACAGCGGCGGTGTGATAGGCGATATCGG".
"CAACGGTAACCGGTAGCGTGGAGTCATGCCCCTGGACCGTCATGCCCAGTGAATCGCCTACCAGCATGAC".
"GTTAATCCCTTCATCGGCAAACAGTTTTGCGAAGCTATAGTCGTAAGCGGTGATGGTTGCGAAACGTTTT".
"TTGTCCTGCTTGCATTTCTGCAATGAAGCAATCGTGGTTGGTTTCATAGCGTATCCTGATAAATGATGTT".
"GTGCTGTCTCGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTATCGCACCGCCGTTGTCT".
"GGCGGTGCGATAGTGTTATCAGGGGTAGGTAACCTGAAAAGTGCTGGTGGCTTTAAAATCCCCCGGTTCT".
"ATAGCAATATTACCGTCTTGTTTTAATGTAGCCTGGAAATGAAGAGGTTGAGAGGTCCCACCGTTGCCAT".
"TATCCGGGAAAATCCCCCCAGTAGTGTCATTTTCTGTTTCATAATCTTTATAAATAGACGTTGCATCATT".
"TGGTTTCAGTACCATTTGTGCACTTTTAGTGTTTTTTAAACCTTCAATCAGTACACCAACGCCTTTTGCT".
"GCGTCATTTCCTGTGAGTGTGTTAGCCAAAAGTTCTTTGCTTACACTACCCACCTTGTTTGACTTGAGTT".
"TTGTTTCTATATTGCGAACACGAATACAGTTCTGTAGCGTGATATCAAAAGGAACCGCTGTTGCGCCATT:".
"01111:::=????====???6111177=::::=???7444=??????====??===??????:::=????".
"??????????=====;96666/.......;=>>>?DDDDDDDDDDDDDDDDDDDDDBBBBDDDDDCCCDD".
"DDDDDDDDC>>>CDDDDDD>>?DDDDDDDDDDDDDCC???CCCCCCDDDDDDDDDDDDDDDDDDDDDDDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD",
# 980 w/ 3 mismatches:
"CCGCGCCCCTGGACTTTGTAGCCACCGAAAATATTCACTGACTGTGGGGTTAGGCCTAAATGAaCACAAA".
"CTGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCCACCAGCCACTCACCGCCTTCAATTTTGACCAT".
"GTTGGCTCCGGCACGCATTACCGTTGCGGCGTTTTCAAAGGCTTGTTCCGGCGTGGCATACGCCATAAAT".
"GGCAGGTCAGCCAGCAGAAGGCAGTTTGGCGCGCCGCGACGCACAGCGGCGGTGTGATAGGCGATATCGG".
"CAACGGTAACCGaTAGCGTGGAGTCATGCCCCTGGACCGTCATGCCCAGTGAATCGCCTACCAGCATGAC".
"GTTAATCCCTTCATCGGCAAACAGTTTTGCGAAGCTATAGTCGTAAGCGGTGATGGTTGCGAAACGTTTT".
"TTGTCCTGCTTGCATTTCTGCAATGAAGCAATCGTGGTTGGTTTCATAGCGTATCCTGATAAATGATGTT".
"GTGCTGTCTCGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTATCGCACCGCCGTTGTCT".
"GGCGGTGCGATAGTGTTATCAGGGGTAGGTAACCTGAAAAGTGCTGGTGGCTTTAAAATCCCCCGGTTCT".
"ATAGCAATATTACCGTCTTGTTTTAATGTAGCCTGGAAATGAAGAGGTTGAGAGGTCCCACCGTTGCCAT".
"TATCCGGGAAAATCCCCCCAGTAGTGTCATTTTCTGTTTCAtAATCTTTATAAATAGACGTTGCATCATT".
"TGGTTTCAGTACCATTTGTGCACTTTTAGTGTTTTTTAAACCTTCAATCAGTACACCAACGCCTTTTGCT".
"GCGTCATTTCCTGTGAGTGTGTTAGCCAAAAGTTCTTTGCTTACACTACCCACCTTGTTTGACTTGAGTT".
"TTGTTTCTATATTGCGAACACGAATACAGTTCTGTAGCGTGATATCAAAAGGAACCGCTGTTGCGCCATT:".
"01111:::=????====???6111177=::::=???7444=??????====??===??????:::=????".
"??????????=====;96666/.......;=>>>?DDDDDDDDDDDDDDDDDDDDDBBBBDDDDDCCCDD".
"DDDDDDDDC>>>CDDDDDD>>?DDDDDDDDDDDDDCC???CCCCCCDDDDDDDDDDDDDDDDDDDDDDDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD",
# 980 w/ a bunch of mismatches:
"CCGaGCCCCaGGACTTTGTAGCCACgGAAAATATTCACTGACTGTGGcGTTAGGCCTAAATGAaCACAAA".
"CTGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCCACCAGCCACTCACCGCCTTCAATTTTGACCAT".
"GTTGGCTCCGGCACGCATTACCGTTGCGGCGTTaTCAAAGGCTTGTTCCGGCGTGGCATACGCCATAAAT".
"GGCAGGTCAGCCAGCAGAAGGCAGTTTGGCGCGCCGCGACGCACAGCGGCGGTGTGATAGGCGATATCGG".
"CAACGGTAACCGaTAGCGTGGAGTCATGCCCCTGGACCGTCATGCCCcGTGAATCGCCTACCAGCATGAC".
"GTTAATCCCTTCATCGGCAAACAGTTTTGCGAAGCTATAGTCGTAAGCGGTGATGGTTGCGAAACGTTTT".
"TTGTCCTGCTTGCATTTCTGCAATGAAGCAATCGTGGTTGGTTTCATAGCGTATCCTGATAAATGATGTT".
"GTGCTGTCTCGCATTTTATCAGTCACATTGGTGGGaGCAATGATTTATCCGTATCGCACCGCCGTTGTCT".
"GGCGGTGCGATAGTGTTATCAGGGGTAGGTAACCTGAAAAGTGCTGGTGGCTTTAAAATCCCCCGGTTCT".
"ATAGCAATATTACCGTCTTGTTTTAATGTAGCCTGGAAATGAAGAGGTTGAGAGGTCCCACCGcTGCCAT".
"TATCCGGGAAAATCCCCCCAGTAGTGTCATTTTCTGTTTCAtAATCTTTATAAATAGACGTTGCATCATT".
"TGGTTTCAGaACCATTTGTGCACTTTTAGTGTTTTTTAAACCTTCAATCAGTACACCAACGCCTTTTGCT".
"GCGTCATTTCCTGTGtGTGTGTTAGCCAAAAGTTCTTTGCTTACACTACCCACCTTGTTTGACTTGAGTT".
"TTGTTTCTATATTGCGAACACGAATACAGTTCTGTAGCGTGATATCAAAAGGAACCGCTGTTGCGCCATT:".
"01111:::=????====???6111177=::::=???7444=??????====??===??????:::=????".
"??????????=====;96666/.......;=>>>?DDDDDDDDDDDDDDDDDDDDDBBBBDDDDDCCCDD".
"DDDDDDDDC>>>CDDDDDD>>?DDDDDDDDDDDDDCC???CCCCCCDDDDDDDDDDDDDDDDDDDDDDDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD".
"DDDDDDDDDDDD;;;CDDDDDCCCCCDDDDDDCCCDDDDDDDDDDDDCCCDDDDDDCCCCCCC:666DDD",
""
);
my @args1 = (
"",
"--best",
"--best -m 1",
"--best --strata -m 1"
);
my @args2 = (
"-v 0",
"-v 1",
"-v 2",
"-v 3",
"-n 0",
"-n 1",
"-n 2",
"-n 3",
"-n 3 -l 25 -e 300"
);
sub btrun {
my ($read, $args, $exalns, $color) = @_;
$args .= $color ? " -C" : "";
my $cmd = "$bowtie $args -c e_coli \"$read\"";
print "\n$cmd\n\n";
my $als = 0;
open BTIE, "$cmd |" || die "Could not open pipe '$cmd |'\n";
while(<BTIE>) {
$als++;
}
close(BTIE);
#$als == $exalns || die "# alignments $als didn't match expected number $exalns\n";
$cmd = "$bowtie_d $args -c e_coli \"$read\"";
print "\n$cmd\n\n";
$als = 0;
open BTIE, "$cmd |" || die "Could not open pipe '$cmd |'\n";
while(<BTIE>) {
$als++;
}
close(BTIE);
#$als == $exalns || die "# alignments $als didn't match expected number $exalns\n";
$cmd = "$bowtie_l $args -c e_coli \"$read\"";
print "\n$cmd\n\n";
$als = 0;
open BTIE, "$cmd |" || die "Could not open pipe '$cmd |'\n";
while(<BTIE>) {
$als++;
}
close(BTIE);
print "PASSED: \"$args\"\n";
}
for my $r (@reads) {
next if $r eq "";
for my $a1 (@args1) {
for my $a2 (@args2) {
btrun($r, "$a1 $a2 -a", 1, 0);
}
}
}
|
