1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300 301 302 303 304 305 306 307 308 309 310 311 312 313 314 315 316 317 318 319 320 321 322 323 324
|
#
# Copyright 2011, Ben Langmead <blangmea@jhsph.edu>
#
# This file is part of Bowtie 2.
#
# Bowtie 2 is free software: you can redistribute it and/or modify
# it under the terms of the GNU General Public License as published by
# the Free Software Foundation, either version 3 of the License, or
# (at your option) any later version.
#
# Bowtie 2 is distributed in the hope that it will be useful,
# but WITHOUT ANY WARRANTY; without even the implied warranty of
# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
# GNU General Public License for more details.
#
# You should have received a copy of the GNU General Public License
# along with Bowtie 2. If not, see <http://www.gnu.org/licenses/>.
#
#
# Makefile for bowtie, bowtie2-build, bowtie2-inspect
#
INC =
GCC_PREFIX = $(shell dirname `which gcc`)
GCC_SUFFIX =
CC = $(GCC_PREFIX)/gcc$(GCC_SUFFIX)
CPP = $(GCC_PREFIX)/g++$(GCC_SUFFIX)
CXX = $(CPP)
HEADERS = $(wildcard *.h)
BOWTIE_PTHREADS = 1
BOWTIE_MM = 1
BOWTIE_SHARED_MEM = 0
# Detect Cygwin or MinGW
WINDOWS = 0
ifneq (,$(findstring CYGWIN,$(shell uname)))
WINDOWS = 1
# POSIX memory-mapped files not currently supported on Windows
BOWTIE_MM = 0
BOWTIE_SHARED_MEM = 0
else
ifneq (,$(findstring MINGW,$(shell uname)))
WINDOWS = 1
# POSIX memory-mapped files not currently supported on Windows
BOWTIE_MM = 0
BOWTIE_SHARED_MEM = 0
endif
endif
MACOS = 0
ifneq (,$(findstring Darwin,$(shell uname)))
MACOS = 1
endif
MM_DEF =
ifeq (1,$(BOWTIE_MM))
MM_DEF = -DBOWTIE_MM
endif
SHMEM_DEF =
ifeq (1,$(BOWTIE_SHARED_MEM))
SHMEM_DEF = -DBOWTIE_SHARED_MEM
endif
PTHREAD_PKG =
PTHREAD_LIB =
PTHREAD_DEF =
ifeq (1,$(BOWTIE_PTHREADS))
PTHREAD_DEF = -DBOWTIE_PTHREADS
ifeq (1,$(WINDOWS))
# pthreads for windows forces us to be specific about the library
PTHREAD_LIB = -lpthreadGC2
PTHREAD_PKG = pthreadGC2.dll
else
# There's also -pthread, but that only seems to work on Linux
PTHREAD_LIB = -lpthread
endif
endif
PREFETCH_LOCALITY = 2
PREF_DEF = -DPREFETCH_LOCALITY=$(PREFETCH_LOCALITY)
LIBS =
SEARCH_LIBS = $(PTHREAD_LIB)
BUILD_LIBS =
SHARED_CPPS = ccnt_lut.cpp ref_read.cpp alphabet.cpp shmem.cpp \
edit.cpp bt2_idx.cpp bt2_io.cpp bt2_util.cpp \
reference.cpp ds.cpp multikey_qsort.cpp limit.cpp
SEARCH_CPPS = qual.cpp pat.cpp sam.cpp \
read_qseq.cpp aligner_seed_policy.cpp \
aligner_seed.cpp aligner_sw.cpp \
aligner_sw_driver.cpp aligner_cache.cpp \
aligner_result.cpp ref_coord.cpp mask.cpp \
pe.cpp aln_sink.cpp dp_framer.cpp \
scoring.cpp presets.cpp unique.cpp \
aligner_bt.cpp sse_util.cpp \
aligner_swsse.cpp outq.cpp \
aligner_swsse_loc_i16.cpp \
aligner_swsse_ee_i16.cpp \
aligner_swsse_loc_u8.cpp \
aligner_swsse_ee_u8.cpp
SEARCH_CPPS_MAIN = $(SEARCH_CPPS) bowtie_main.cpp
BUILD_CPPS = diff_sample.cpp
BUILD_CPPS_MAIN = $(BUILD_CPPS) bowtie_build_main.cpp
SEARCH_FRAGMENTS = $(wildcard search_*_phase*.c)
VERSION = $(shell cat VERSION)
EXTRA_FLAGS =
ifeq (1,$(ENABLE_SSE))
ARCH := $(shell getconf LONG_BIT)
UNAME := $(shell uname -m )
# Convert BITS=?? to a -m flag
BITS=32
BITS_FLAG = -m32
ifeq (64,$(ARCH))
BITS=64
BITS_FLAG = -m64
endif
ifeq ($(UNAME),'Power Macintosh')
ifeq (32,$(BITS))
BITS_FLAG = -m32
SSE_FLAG =
endif
ifeq (64,$(BITS))
BITS_FLAG = -m64
SSE_FLAG =
endif
endif
SSE_FLAG = -msse2
endif
DEBUG_FLAGS = -O0 -g3 $(BITS_FLAG) $(SSE_FLAG)
DEBUG_DEFS = -DCOMPILER_OPTIONS="\"$(DEBUG_FLAGS) $(EXTRA_FLAGS)\""
RELEASE_FLAGS = -O3 $(BITS_FLAG) $(SSE_FLAG) -funroll-loops -g3
RELEASE_DEFS = -DCOMPILER_OPTIONS="\"$(RELEASE_FLAGS) $(EXTRA_FLAGS)\""
NOASSERT_FLAGS = -DNDEBUG
FILE_FLAGS = -D_LARGEFILE_SOURCE -D_FILE_OFFSET_BITS=64 -D_GNU_SOURCE
BOWTIE2_BIN_LIST = bowtie2-build \
bowtie2-align \
bowtie2-inspect
BOWTIE2_BIN_LIST_AUX = bowtie2-build-debug \
bowtie2-align-debug \
bowtie2-inspect-debug
GENERAL_LIST = $(wildcard scripts/*.sh) \
$(wildcard scripts/*.pl) \
doc/manual.html \
doc/README \
doc/style.css \
$(wildcard example/index/*.bt2) \
$(wildcard example/reads/*.fq) \
$(wildcard example/reads/*.pl) \
example/reference/lambda_virus.fa \
$(PTHREAD_PKG) \
bowtie2 \
AUTHORS \
COPYING \
NEWS \
MANUAL \
MANUAL.markdown \
TUTORIAL \
VERSION
# This is helpful on Windows under MinGW/MSYS, where Make might go for
# the Windows FIND tool instead.
FIND=$(shell which find)
SRC_PKG_LIST = $(wildcard *.h) \
$(wildcard *.hh) \
$(wildcard *.c) \
$(wildcard *.cpp) \
doc/strip_markdown.pl \
Makefile \
$(GENERAL_LIST)
BIN_PKG_LIST = $(GENERAL_LIST)
.PHONY: all allall both both-debug
all: $(BOWTIE2_BIN_LIST)
allall: $(BOWTIE2_BIN_LIST) $(BOWTIE2_BIN_LIST_AUX)
both: bowtie2 bowtie2-build
both-debug: bowtie2-align-debug bowtie2-build-debug
DEFS=-fno-strict-aliasing \
-DBOWTIE2_VERSION="\"`cat VERSION`\"" \
-DBUILD_HOST="\"`hostname`\"" \
-DBUILD_TIME="\"`date`\"" \
-DCOMPILER_VERSION="\"`$(CXX) -v 2>&1 | tail -1`\"" \
$(FILE_FLAGS) \
$(PTHREAD_DEF) \
$(PREF_DEF) \
$(MM_DEF) \
$(SHMEM_DEF)
#
# bowtie2-build targets
#
bowtie2-build: bt2_build.cpp $(SHARED_CPPS) $(HEADERS)
$(CXX) $(RELEASE_FLAGS) $(RELEASE_DEFS) $(EXTRA_FLAGS) \
$(DEFS) -DBOWTIE2 $(NOASSERT_FLAGS) -Wall \
$(INC) \
-o $@ $< \
$(SHARED_CPPS) $(BUILD_CPPS_MAIN) \
$(LIBS) $(BUILD_LIBS)
bowtie2-build-debug: bt2_build.cpp $(SHARED_CPPS) $(HEADERS)
$(CXX) $(DEBUG_FLAGS) $(DEBUG_DEFS) $(EXTRA_FLAGS) \
$(DEFS) -DBOWTIE2 -Wall \
$(INC) \
-o $@ $< \
$(SHARED_CPPS) $(BUILD_CPPS_MAIN) \
$(LIBS) $(BUILD_LIBS)
#
# bowtie targets
#
bowtie2-align: bt2_search.cpp $(SEARCH_CPPS) $(SHARED_CPPS) $(HEADERS) $(SEARCH_FRAGMENTS)
$(CXX) $(RELEASE_FLAGS) $(RELEASE_DEFS) $(EXTRA_FLAGS) \
$(DEFS) -DBOWTIE2 $(NOASSERT_FLAGS) -Wall \
$(INC) \
-o $@ $< \
$(SHARED_CPPS) $(SEARCH_CPPS_MAIN) \
$(LIBS) $(SEARCH_LIBS)
bowtie2-align-debug: bt2_search.cpp $(SEARCH_CPPS) $(SHARED_CPPS) $(HEADERS) $(SEARCH_FRAGMENTS)
$(CXX) $(DEBUG_FLAGS) \
$(DEBUG_DEFS) $(EXTRA_FLAGS) \
$(DEFS) -DBOWTIE2 -Wall \
$(INC) \
-o $@ $< \
$(SHARED_CPPS) $(SEARCH_CPPS_MAIN) \
$(LIBS) $(SEARCH_LIBS)
#
# bowtie2-inspect targets
#
bowtie2-inspect: bt2_inspect.cpp $(HEADERS) $(SHARED_CPPS)
$(CXX) $(RELEASE_FLAGS) \
$(RELEASE_DEFS) $(EXTRA_FLAGS) \
$(DEFS) -DBOWTIE2 -DBOWTIE_INSPECT_MAIN -Wall \
$(INC) -I . \
-o $@ $< \
$(SHARED_CPPS) \
$(LIBS)
bowtie2-inspect-debug: bt2_inspect.cpp $(HEADERS) $(SHARED_CPPS)
$(CXX) $(DEBUG_FLAGS) \
$(DEBUG_DEFS) $(EXTRA_FLAGS) \
$(DEFS) -DBOWTIE2 -DBOWTIE_INSPECT_MAIN -Wall \
$(INC) -I . \
-o $@ $< \
$(SHARED_CPPS) \
$(LIBS)
.PHONY: bowtie2-src
bowtie2-src: $(SRC_PKG_LIST)
chmod a+x scripts/*.sh scripts/*.pl
mkdir .src.tmp
mkdir .src.tmp/bowtie2-$(VERSION)
zip tmp.zip $(SRC_PKG_LIST)
mv tmp.zip .src.tmp/bowtie2-$(VERSION)
cd .src.tmp/bowtie2-$(VERSION) ; unzip tmp.zip ; rm -f tmp.zip
cd .src.tmp ; zip -r bowtie2-$(VERSION)-source.zip bowtie2-$(VERSION)
cp .src.tmp/bowtie2-$(VERSION)-source.zip .
rm -rf .src.tmp
.PHONY: bowtie2-bin
bowtie2-bin: $(BIN_PKG_LIST) $(BOWTIE2_BIN_LIST) $(BOWTIE2_BIN_LIST_AUX)
chmod a+x scripts/*.sh scripts/*.pl
rm -rf .bin.tmp
mkdir .bin.tmp
mkdir .bin.tmp/bowtie2-$(VERSION)
if [ -f bowtie.exe ] ; then \
zip tmp.zip $(BIN_PKG_LIST) $(addsuffix .exe,$(BOWTIE2_BIN_LIST) $(BOWTIE2_BIN_LIST_AUX)) ; \
else \
zip tmp.zip $(BIN_PKG_LIST) $(BOWTIE2_BIN_LIST) $(BOWTIE2_BIN_LIST_AUX) ; \
fi
mv tmp.zip .bin.tmp/bowtie2-$(VERSION)
cd .bin.tmp/bowtie2-$(VERSION) ; unzip tmp.zip ; rm -f tmp.zip
cd .bin.tmp ; zip -r bowtie2-$(VERSION)-$(BITS).zip bowtie2-$(VERSION)
cp .bin.tmp/bowtie2-$(VERSION)-$(BITS).zip .
rm -rf .bin.tmp
bowtie2-seeds-debug: aligner_seed.cpp ccnt_lut.cpp alphabet.cpp aligner_seed.h bt2_idx.cpp bt2_io.cpp
$(CXX) $(DEBUG_FLAGS) \
$(DEBUG_DEFS) $(EXTRA_FLAGS) \
-DSCAN_MAIN \
$(DEFS) -Wall \
$(INC) -I . \
-o $@ $< \
aligner_seed.cpp bt2_idx.cpp ccnt_lut.cpp alphabet.cpp bt2_io.cpp \
$(LIBS)
.PHONY: doc
doc: doc/manual.html MANUAL
doc/manual.html: MANUAL.markdown
echo "<h1>Table of Contents</h1>" > .tmp.head
pandoc -T "Bowtie 2 Manual" -B .tmp.head \
--css style.css -o $@ \
--from markdown --to HTML \
--table-of-contents $^
MANUAL: MANUAL.markdown
perl doc/strip_markdown.pl < $^ > $@
.PHONY: clean
clean:
rm -f $(BOWTIE2_BIN_LIST) $(BOWTIE2_BIN_LIST_AUX) \
$(addsuffix .exe,$(BOWTIE2_BIN_LIST) $(BOWTIE2_BIN_LIST_AUX)) \
bowtie2-src.zip bowtie2-bin.zip
rm -f core.* .tmp.head
rm -rf *.dSYM
|