File: bt2_util.cpp

package info (click to toggle)
bowtie2 2.4.2-2
  • links: PTS, VCS
  • area: main
  • in suites: bullseye
  • size: 27,104 kB
  • sloc: cpp: 60,331; perl: 7,540; sh: 1,111; python: 949; makefile: 520; ansic: 122
file content (219 lines) | stat: -rw-r--r-- 6,437 bytes parent folder | download | duplicates (7)
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
/*
 * Copyright 2011, Ben Langmead <langmea@cs.jhu.edu>
 *
 * This file is part of Bowtie 2.
 *
 * Bowtie 2 is free software: you can redistribute it and/or modify
 * it under the terms of the GNU General Public License as published by
 * the Free Software Foundation, either version 3 of the License, or
 * (at your option) any later version.
 *
 * Bowtie 2 is distributed in the hope that it will be useful,
 * but WITHOUT ANY WARRANTY; without even the implied warranty of
 * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
 * GNU General Public License for more details.
 *
 * You should have received a copy of the GNU General Public License
 * along with Bowtie 2.  If not, see <http://www.gnu.org/licenses/>.
 */

#include <string>
#include <stdexcept>
#include <iostream>
#include <fstream>
#include <stdlib.h>
#include <string.h>
#include "bt2_idx.h"

///////////////////////////////////////////////////////////////////////
//
// Functions for printing and sanity-checking Ebwts
//
///////////////////////////////////////////////////////////////////////

/**
 * Check that the ebwt array is internally consistent up to (and not
 * including) the given side index by re-counting the chars and
 * comparing against the embedded occ[] arrays.
 */
void Ebwt::sanityCheckUpToSide(TIndexOff upToSide) const {
	assert(isInMemory());
	TIndexOffU occ[] = {0, 0, 0, 0};
	ASSERT_ONLY(TIndexOffU occ_save[] = {0, 0, 0, 0});
	TIndexOffU cur = 0; // byte pointer
	const EbwtParams& eh = this->_eh;
	bool fw = false;
	while(cur < (TIndexOffU)(upToSide * eh._sideSz)) {
		assert_leq(cur + eh._sideSz, eh._ebwtTotLen);
		for(uint32_t i = 0; i < eh._sideBwtSz; i++) {
			uint8_t by = this->ebwt()[cur + (fw ? i : eh._sideBwtSz-i-1)];
			for(int j = 0; j < 4; j++) {
				// Unpack from lowest to highest bit pair
				int twoBit = unpack_2b_from_8b(by, fw ? j : 3-j);
				occ[twoBit]++;
			}
			assert_eq(0, (occ[0] + occ[1] + occ[2] + occ[3]) % 4);
		}
		assert_eq(0, (occ[0] + occ[1] + occ[2] + occ[3]) % eh._sideBwtLen);
		// Finished forward bucket; check saved [A], [C], [G] and [T]
		// against the uint32_ts encoded here
		ASSERT_ONLY(const TIndexOffU *u_ebwt = reinterpret_cast<const TIndexOffU*>(&ebwt()[cur + eh._sideBwtSz]));
		ASSERT_ONLY(TIndexOffU as = u_ebwt[0]);
		ASSERT_ONLY(TIndexOffU cs = u_ebwt[1]);
		ASSERT_ONLY(TIndexOffU gs = u_ebwt[2]);
		ASSERT_ONLY(TIndexOffU ts = u_ebwt[3]);
		assert(as == occ_save[0] || as == occ_save[0]-1);
		assert_eq(cs, occ_save[1]);
		assert_eq(gs, occ_save[2]);
		assert_eq(ts, occ_save[3]);
#ifndef NDEBUG
		occ_save[0] = occ[0];
		occ_save[1] = occ[1];
		occ_save[2] = occ[2];
		occ_save[3] = occ[3];
#endif
		cur += eh._sideSz;
	}
}

/**
 * Sanity-check various pieces of the Ebwt
 */
void Ebwt::sanityCheckAll(int reverse) const {
	const EbwtParams& eh = this->_eh;
	assert(isInMemory());
	// Check ftab
	for(TIndexOffU i = 1; i < eh._ftabLen; i++) {
		assert_geq(this->ftabHi(i), this->ftabLo(i-1));
		assert_geq(this->ftabLo(i), this->ftabHi(i-1));
		assert_leq(this->ftabHi(i), eh._bwtLen+1);
	}
	assert_eq(this->ftabHi(eh._ftabLen-1), eh._bwtLen);
	
	// Check offs
	TIndexOff seenLen = (eh._bwtLen + 31) >> ((TIndexOffU)5);
	TIndexOff *seen;
	try {
		seen = new TIndexOff[seenLen]; // bitvector marking seen offsets
	} catch(bad_alloc& e) {
		cerr << "Out of memory allocating seen[] at " << __FILE__ << ":" << __LINE__ << endl;
		throw e;
	}
	memset(seen, 0, OFF_SIZE * seenLen);
	TIndexOffU offsLen = eh._offsLen;
	for(TIndexOffU i = 0; i < offsLen; i++) {
		assert_lt(this->offs()[i], eh._bwtLen);
		TIndexOff w = this->offs()[i] >> 5;
		TIndexOff r = this->offs()[i] & 31;
		assert_eq(0, (seen[w] >> r) & 1); // shouldn't have been seen before
		seen[w] |= (1 << r);
	}
	delete[] seen;
	
	// Check nPat
	assert_gt(this->_nPat, 0);
	
	// Check plen, flen
	for(TIndexOffU i = 0; i < this->_nPat; i++) {
		assert_geq(this->plen()[i], 0);
	}
	
	// Check rstarts
	if(this->rstarts() != NULL) {
		for(TIndexOffU i = 0; i < this->_nFrag-1; i++) {
			assert_gt(this->rstarts()[(i+1)*3], this->rstarts()[i*3]);
			if(reverse == REF_READ_REVERSE) {
				assert(this->rstarts()[(i*3)+1] >= this->rstarts()[((i+1)*3)+1]);
			} else {
				assert(this->rstarts()[(i*3)+1] <= this->rstarts()[((i+1)*3)+1]);
			}
		}
	}
	
	// Check ebwt
	sanityCheckUpToSide(eh._numSides);
	VMSG_NL("Ebwt::sanityCheck passed");
}

/**
 * Transform this Ebwt into the original string in linear time by using
 * the LF mapping to walk backwards starting at the row correpsonding
 * to the end of the string.  The result is written to s.  The Ebwt
 * must be in memory.
 */
void Ebwt::restore(SString<char>& s) const {
	assert(isInMemory());
	s.resize(this->_eh._len);
	TIndexOffU jumps = 0;
	TIndexOffU i = this->_eh._len; // should point to final SA elt (starting with '$')
	SideLocus l(i, this->_eh, this->ebwt());
	while(i != _zOff) {
		assert_lt(jumps, this->_eh._len);
		//if(_verbose) cout << "restore: i: " << i << endl;
		// Not a marked row; go back a char in the original string
		TIndexOffU newi = mapLF(l ASSERT_ONLY(, false));
		assert_neq(newi, i);
		s[this->_eh._len - jumps - 1] = rowL(l);
		i = newi;
		l.initFromRow(i, this->_eh, this->ebwt());
		jumps++;
	}
	assert_eq(jumps, this->_eh._len);
}

/**
 * Check that this Ebwt, when restored via restore(), matches up with
 * the given array of reference sequences.  For sanity checking.
 */
void Ebwt::checkOrigs(
	const EList<SString<char> >& os,
	bool color,
	bool mirror) const
{
	SString<char> rest;
	restore(rest);
	TIndexOffU restOff = 0;
	size_t i = 0, j = 0;
	if(mirror) {
		// TODO: FIXME
		return;
	}
	while(i < os.size()) {
		size_t olen = os[i].length();
		int lastorig = -1;
		for(; j < olen; j++) {
			size_t joff = j;
			if(mirror) joff = olen - j - 1;
			if((int)os[i][joff] == 4) {
				// Skip over Ns
				lastorig = -1;
				if(!mirror) {
					while(j < olen && (int)os[i][j] == 4) j++;
				} else {
					while(j < olen && (int)os[i][olen-j-1] == 4) j++;
				}
				j--;
				continue;
			}
			if(lastorig == -1 && color) {
				lastorig = os[i][joff];
				continue;
			}
			if(color) {
				assert_neq(-1, lastorig);
				assert_eq(dinuc2color[(int)os[i][joff]][lastorig], rest[restOff]);
			} else {
				assert_eq(os[i][joff], rest[restOff]);
			}
			lastorig = (int)os[i][joff];
			restOff++;
		}
		if(j == os[i].length()) {
			// Moved to next sequence
			i++;
			j = 0;
		} else {
			// Just jumped over a gap
		}
	}
}