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/*************************************************************************************
The MIT License
BWA-MEM2 (Sequence alignment using Burrows-Wheeler Transform),
Copyright (C) 2019 Intel Corporation, Heng Li.
Permission is hereby granted, free of charge, to any person obtaining
a copy of this software and associated documentation files (the
"Software"), to deal in the Software without restriction, including
without limitation the rights to use, copy, modify, merge, publish,
distribute, sublicense, and/or sell copies of the Software, and to
permit persons to whom the Software is furnished to do so, subject to
the following conditions:
The above copyright notice and this permission notice shall be
included in all copies or substantial portions of the Software.
THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND,
EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF
MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND
NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS
BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN
ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN
CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE
SOFTWARE.
Authors: Vasimuddin Md <vasimuddin.md@intel.com>; Sanchit Misra <sanchit.misra@intel.com>.
*****************************************************************************************/
#include<stdio.h>
#include<stdlib.h>
#include<stdint.h>
#include "FMI_search.h"
#include <omp.h>
#include <string.h>
#ifdef VTUNE_ANALYSIS
#include <ittnotify.h>
#endif
#define MAX_NUM_QUERIES 1000000
#define QUERY_DB_SIZE 500000000L
int myrank, num_ranks;
int32_t read_smem2_input(char *fname, char *query_seq, int16_t *query_pos_array, int32_t *min_intv_array, int32_t readlen)
{
FILE *smem2_fp = fopen(fname, "r");
assert(smem2_fp != NULL);
int numReads = 0;
char line[1024];
while(fgets(line, 1024, smem2_fp) != NULL)
{
//printf("0.0\n");
int32_t rlen = strlen(line);
//printf("1.0\n");
if(rlen != (readlen + 1))
{
printf("ERROR! len - 1 = %d, readlen = %d\n", rlen - 1, readlen);
exit(1);
}
//printf("2.0\n");
memcpy(query_seq + numReads * readlen, line, readlen);
//printf("3.0\n");
fscanf(smem2_fp, "%hd", query_pos_array + numReads);
//printf("4.0\n");
fscanf(smem2_fp, "%d", min_intv_array + numReads);
//printf("4.1\n");
//printf("numReads = %d, numReads * readlen = %d, query_x = %d, min_intv = %dhi\n", numReads, numReads * readlen, query_pos_array[numReads], min_intv_array[numReads]);
numReads++;
//printf("5.0\n");
fgets(line, 1024, smem2_fp);
//printf("6.0\n");
}
printf("numReads = %d\n", numReads);
//printf("Exiting\n");
fflush(stdout);
fclose(smem2_fp);
return numReads;
}
int main(int argc, char **argv) {
#ifdef VTUNE_ANALYSIS
__itt_pause();
#endif
if(argc!=7)
{
printf("Need seven arguments : ref_file query_set batch_size readlength minSeedLen n_threads\n");
return 1;
}
char *query_seq=(char *)malloc(QUERY_DB_SIZE*sizeof(char));
int16_t *query_pos_array = (int16_t *)_mm_malloc(MAX_NUM_QUERIES * sizeof(int16_t), 64);
int32_t *min_intv_array = (int32_t *)_mm_malloc(MAX_NUM_QUERIES * sizeof(int32_t), 64);
int32_t *rid_array = (int32_t *)_mm_malloc(MAX_NUM_QUERIES * sizeof(int32_t), 64);
int readlength=atoi(argv[4]);
assert(readlength > 0);
assert(readlength < 10000);
long numReads;
numReads=read_smem2_input(argv[2], query_seq, query_pos_array, min_intv_array, readlength);
assert(numReads > 0);
assert(numReads * readlength < QUERY_DB_SIZE);
int32_t *query_cum_len_ar = (int32_t *)_mm_malloc(numReads * sizeof(int32_t), 64);
bseq1_t *seqs = (bseq1_t *)_mm_malloc(numReads * sizeof(bseq1_t), 64);
FMI_search *fmiSearch = new FMI_search(argv[1]);
uint8_t *enc_qdb=(uint8_t *)malloc(numReads*readlength*sizeof(uint8_t));
long cind,st;
uint64_t r;
for (st=0; st < numReads; st++) {
query_cum_len_ar[st] = st * readlength;
seqs[st].l_seq = readlength;
cind=st*readlength;
for(r = 0; r < readlength; ++r) {
switch(query_seq[r+cind])
{
case '0': enc_qdb[r+cind]=0;
break;
case '1': enc_qdb[r+cind]=1;
break;
case '2': enc_qdb[r+cind]=2;
break;
case '3': enc_qdb[r+cind]=3;
break;
default: enc_qdb[r+cind]=4;
}
//printf("%c %d\n", query_seq[r+cind], enc_qdb[r + cind]);
//printf("%d", enc_qdb[r + cind]);
}
}
int batch_size=0;
batch_size=atoi(argv[3]);
SMEM *matchArray = (SMEM *)_mm_malloc(numReads * readlength * sizeof(SMEM), 64);
int32_t minSeedLen = atoi(argv[5]);
int numthreads=atoi(argv[6]);
int64_t numTotalSmem[numthreads];
#pragma omp parallel num_threads(numthreads)
{
int tid = omp_get_thread_num();
if(tid == 0)
printf("Running %d threads\n", omp_get_num_threads());
numTotalSmem[tid] = 0;
}
int32_t i;
for(i = 0; i < numReads; i++)
{
rid_array[i] = i;
}
int64_t startTick, endTick;
#ifdef VTUNE_ANALYSIS
__itt_resume();
#endif
printf("before getSMEM\n");
fflush(stdout);
startTick = __rdtsc();
numthreads = 1;
fmiSearch->getSMEMsOnePosOneThread(enc_qdb,
query_pos_array,
min_intv_array,
rid_array,
numReads,
batch_size,
seqs,
query_cum_len_ar,
readlength,
minSeedLen,
matchArray,
numTotalSmem);
endTick = __rdtsc();
#ifdef VTUNE_ANALYSIS
__itt_pause();
#endif
printf("Consumed: %ld cycles\n", endTick - startTick);
int64_t totalSmem = 0;
int tid;
for(tid = 0; tid < numthreads; tid++)
{
totalSmem += numTotalSmem[tid];
}
printf("totalSmems = %ld\n", totalSmem);
fmiSearch->sortSMEMs(matchArray,
numTotalSmem,
numReads,
readlength,
numthreads);
int32_t perThreadQuota = (numReads + (numthreads - 1)) / numthreads;
for(tid = 0; tid < numthreads; tid++)
{
int32_t first = tid * perThreadQuota;
SMEM *myMatchArray = matchArray + first * readlength;
int64_t i = 0;
int64_t rid;
for(rid = 0; rid < numReads; rid++)
{
while((i < numTotalSmem[tid]) && (myMatchArray[i].rid == rid))
{
SMEM smem = myMatchArray[i];
//printf("\n%u: ", smem.rid);
printf("[ %u %u %u %u %u ] ", smem.k, smem.l, smem.s, smem.m, smem.n + 1);
i++;
}
printf("\n");
}
}
free(query_seq);
free(enc_qdb);
_mm_free(rid_array);
_mm_free(query_pos_array);
_mm_free(matchArray);
_mm_free(min_intv_array);
delete fmiSearch;
return 0;
}
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