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#!/usr/local/bin/perl
# -*-Perl-*-
#######################################################################
# This software has been created by Genome Research Limited (GRL). #
# GRL hereby grants permission to use, copy, modify and distribute #
# this software and its documentation for non-commercial purposes #
# without fee at the user's own risk on the basis set out below. #
# GRL neither undertakes nor accepts any duty whether contractual or #
# otherwise in connection with the software, its use or the use of #
# any derivative, and makes no representations or warranties, express #
# or implied, concerning the software, its suitability, fitness for #
# a particular purpose or non-infringement. #
# In no event shall the authors of the software or GRL be responsible #
# or liable for any loss or damage whatsoever arising in any way #
# directly or indirectly out of the use of this software or its #
# derivatives, even if advised of the possibility of such damage. #
# Our software can be freely distributed under the conditions set out #
# above, and must contain this copyright notice. #
#######################################################################
#
package TestTools;
use Carp;
use strict;
use FileHandle;
$|=1;
sub new {
my $type = shift;
my %params = @_;
my $self = {};
$self->{verbose} = $params{verbose};
$self->{CAF} = {
DNA => {},
DNALen => {},
BaseQuality => {},
BaseQualityLen => {}
};
$self->{test} = $params{tests_to_run};
if($self->{test}) {
$self->{pass} = 0;
$self->{fail} = 0;
$self->{ontest} = 0;
$self->{test_src_dir} = '../../src';
($self->{program}) = (caller())[1] =~ /test_(\S+)/;
}
my $ref = bless $self, $type;
if($self->{test}) {
$ref->enter_test_dir();
select $ref->open_test_log();
}
return $ref;
}
sub enter_test_dir {
my ($self) = @_;
my $dir = $self->{program} . "-test";
unless(chdir("$dir")) {
++$self->{fail};
&print_test_results();
print "ERROR: Unable to enter directory $dir.$!\n";
die;
}
}
sub readCafFile {
my ($self, $file) = @_;
my $fileRef = (ref($file)) ? $file : (FileHandle->new("< $file"));
my $size = -s $fileRef;
$size = 1 unless ($size);
my ($name, $class,$failed_dup);
my @x;
while (<$fileRef>) {
s/\/\/.*// ; # ace file comments
if ( /^DNA\s+(:\s+)?(\S+)/ ) {
$name = $2;
@x = ();
while (<$fileRef>) {
last if /^\s*$/ ;
s/\s+//g ; # remove whitespace
push(@x,$_);
}
$self->{CAF}->{DNA}->{$name} = join("",@x)
unless ($self->{CAF}->{DNA}->{$name} && $self->_reportDuplicate($name, 'DNA',\$failed_dup));
$self->{CAF}->{DNALen}->{$name} = scalar(@x);
} elsif ( /BaseQuality\s+(:\s+)?(\S+)/ ) {
$name = $2;
@x = ();
while (<$fileRef>) {
last if /^\s*$/;
chomp;
push(@x, $_);
}
$self->{CAF}->{BaseQuality}->{$name} = join(" ", @x)
unless ($self->{CAF}->{BaseQuality}->{$name} && $self->_reportDuplicate($name, 'base quality',\$failed_dup));
$self->{CAF}->{BaseQualityLen}->{$name} = scalar(@x);
} elsif ( /^(\S+)\s+(:\s+)?(\S+)/ ) {
$class = $1;
$name = $3;
$self->{CAF}->{$class} = {} unless $self->{CAF}->{$class};
@x = ();
while (<$fileRef>) {
last if /^\s*$/ ;
s/\\\n$// ; # continuation lines
push(@x,$_);
}
@x = sort @x;
if (defined($self->{CAF}->{$class}->{$name})) {
$self->{CAF}->{$class}->{$name} .= join("",@x) unless $self->_reportDuplicate($name, $class,\$failed_dup);
} else {
$self->{CAF}->{$class}->{$name} = join("",@x);
# combine sequence string into one nospace line for equality.
$self->{CAF}->{$class}->{$name} =~ s/[\n|\s+]//g;
}
}
last if eof($fileRef);
}
return $failed_dup;
}
sub _reportDuplicate {
my ($self, $name, $class,$fc) = @_;
print "Found duplicate $class entry $name in CAF file.\n";
++$$fc;
}
sub contigsList {
my ($self) = @_;
confess "Wrong type" unless ref($self);
return grep($self->{CAF}->{Sequence}->{$_} =~ /Is_contig\n/, keys(%{$self->{CAF}->{Sequence}}));
}
sub readsList {
my ($self) = @_;
confess "Wrong type" unless ref($self);
return grep($self->{CAF}->{Sequence}->{$_} =~ /Is_read\n/, keys(%{$self->{CAF}->{Sequence}}));
}
sub _warnError {
my ($msg, $retval) = @_;
print $msg;
return $retval;
}
sub checkForMissingInfo {
my ($self) = @_;
my $mustHaveBaseQuality = 1;
my $errorCount = 0;
my $key = 0;
$self->test_header("Checking CAF data for missing entries");
foreach $key (keys %{$self->{CAF}->{DNA}}) {
unless ($mustHaveBaseQuality && $self->{CAF}->{BaseQuality}->{$key}) {
$errorCount += &_warnError("DNA entry $key has no corresponding base quality entry\n", 1);
}
unless ($self->{CAF}->{Sequence}->{$key}) {
$errorCount += &_warnError("DNA entry $key has no corresponding sequence entry\n", 1);
}
}
foreach $key (keys %{$self->{CAF}->{BaseQuality}}) {
unless ($self->{CAF}->{DNA}->{$key}) {
$errorCount += &_warnError("BaseQuality entry $key has no corresponding DNA entry\n", 1);
}
unless ($self->{CAF}->{Sequence}->{$key}) {
$errorCount += &_warnError("BaseQuality entry $key has no corresponding sequence entry\n", 1);
}
}
foreach $key (keys %{$self->{CAF}->{Sequence}}) {
unless ($self->{CAF}->{DNA}->{$key}) {
$errorCount += &_warnError("Sequence entry $key has no corresponding DNA entry\n", 1);
}
unless ($mustHaveBaseQuality && $self->{CAF}->{BaseQuality}->{$key}) {
$errorCount += &_warnError("Sequence entry $key has no corresponding base quality entry\n", 1);
}
}
(!$errorCount) ? $self->test_result(1) : $self->test_result(0);
}
sub checkRawSequences {
# Checks Sequence entries for raw reads
my ($self) = @_;
$self->test_header("Checking raw sequence entries for missing information");
my $key;
my $errorCount;
foreach $key ($self->readsList()) {
if ($self->checkRawSequence($key)) { $errorCount++; }
}
(!$errorCount) ? $self->test_result(1) : $self->test_result(0);
}
sub checkRawSequence {
my ($self, $name) = @_;
my $entry = $self->{CAF}->{Sequence}->{$name};
my $retVal = 0;
unless ($entry =~ /Is_read/) { $retVal += &_warnError("$name is not a read\n", 1); }
unless ($entry =~ /Template/) { $retVal += &_warnError("$name does not have a template entry\n", 1); }
unless ($entry =~ /Insert_size/) { $retVal += &_warnError("$name does not have an insert size entry\n", 1); }
unless ($entry =~ /Primer/) { $retVal += &_warnError("$name does not have a primer entry\n", 1); }
unless ($entry =~ /Strand/) { $retVal += &_warnError("$name does not have a strand entry\n", 1); }
unless ($entry =~ /Dye/) { $retVal += &_warnError("$name does not have a dye entry\n", 1); }
return $retVal;
}
#
# Check for nulls within structures.
#
sub check_null {
my($self) = @_;
$self->test_header("Checking caf file for NULL's");
foreach my $type (keys %{$self->{CAF}}) {
foreach my $key (keys %{$self->{CAF}->{$type}}) {
my $entry = $self->{CAF}->{$type}->{$key};
if($entry =~ /\000/g) {
print "Null(s) located within data for $key ($type).\n";
$self->test_result(0);
return;
}
}
}
$self->test_result(1);
}
#
# Ensure sequence length matches basequality length.
#
sub check_seq_quality_length {
my ($self) = @_;
$self->test_header("Checking seq length matches basequality length");
foreach my $rd ($self->readsList()) {
my $dna = length($self->{CAF}->{DNA}->{$rd});
my $qual = (split/ /,$self->{CAF}->{BaseQuality}->{$rd});
if($dna != $qual) {
print "Sequence length and basequality differ for entry $rd (seq: $dna | qual: $qual).\n";
$self->test_result(0);
return;
}
}
$self->test_result(1);
}
sub print_test_results {
my ($self) = @_;
if($self->{pass} != $self->{test}) {
printf STDERR ("%s\n-*- %s: -*-\n%s out of %s tests passed\n%s\n\n\n",
'#'x35,$self->{program},$self->{pass},$self->{test},'#'x35);
}else{
printf STDERR ("%s\n-*- %s: -*-\nAll tests passed\n%s\n\n\n",'#'x35,$self->{program},'#'x35);
}
}
sub run_installed {
my($self,$args) = @_;
$self->test_header("Installed program output");
if(system("$self->{installed_dir}/$self->{program} $args")) {
print "Error: Failure in system call. ($self->{installed_dir}/$self->{program} $args).$!\n";
$self->test_result(0);
die;
}
$self->test_result(1);
}
sub run_tested {
my($self,$args) = @_;
$self->test_header("Test program output");
if(system("$self->{test_src_dir}/$self->{program} $args")) {
print "Error: Failure in system call. ($self->{test_src_dir}/$self->{program} $args).$!\n";
$self->test_result(0);
die;
}
$self->test_result(1);
}
sub test_header {
my($self,$string) = @_;
my $len = length($string) - 3;
printf(STDERR "Test %d: %s %s ",++$self->{ontest},$string,"."x(50-$len));
}
sub test_result {
my($self,$result) = @_;
if($result==1) {
++$self->{pass};
print STDERR "Pass\n";
}else{
++$self->{fail};
print STDERR "Fail\n";
}
}
sub open_test_log {
my ($self) = @_;
unless(open(LOG,">error_log")) {
warn "Unable to open error log.$!\n";
return \*STDERR;
}
select((select(LOG), $|=1)[0]);
return \*LOG;
}
sub compare_output {
my($self,$insthash,$testhash,$str) = @_;
$self->test_header($str);
my $rows = 0;
my $found = 0;
foreach my $key (keys %$insthash) {
++$rows;
if(exists $$testhash{$key}) {
++$found;
}
}
if($rows != $found) {
print "Row mismatch! Installed contains $rows rows, test version contains $found rows.\n";
$self->test_result(0);
return;
}
$self->test_result(1);
}
sub read_template_output {
my($self,$hashref,$file,$str) = @_;
$self->test_header($str);
unless(open(F,"$file")) {
print "Unable to open file $file for reading.$!\n";
$self->test_result(0);
}
while(defined(my $line = <F>)) {
chomp($line);
# new version has an extra column, we'll ignore that .
($line) = $line =~ /^(.*\d+\s+\d+\s+\d+\s+\d+\s+\S+\s+\S+)/;
$$hashref{$line}++;
}
close(F);
$self->test_result(1);
}
sub read_standard_output {
my($self) = @_;
$self->test_header('Reading standard output file');
my $selfref = (ref($self))->new();
my $fc = $selfref->readCafFile("../test_data/$self->{program}.caf");
(!$fc) ? $self->test_result(1) : $self->test_result(0);
return $selfref;
}
sub compare_against_caf {
my($self,$stdcaf) = @_;
$self->test_header('Comparing test output');
my $fc;
foreach my $class ('DNALen','BaseQualityLen','Sequence') {
foreach my $item ($self->readsList(), $self->contigsList()) {
if($class eq 'DNALen' || $class eq 'BaseQualityLen') {
if($self->{CAF}->{$class}->{$item} != $stdcaf->{CAF}->{$class}->{$item}) {
print "test $class -> $item = $self->{CAF}->{$class}->{$item} : standard $class -> $item = $stdcaf->{CAF}->{$class}->{$item}\n";
++$fc;
}
}else{
if($self->{CAF}->{$class}->{$item} ne $stdcaf->{CAF}->{$class}->{$item}) {
print "sequence class difference for $item. \n$self->{CAF}->{$class}->{$item} ne $stdcaf->{CAF}->{$class}->{$item}";
++$fc;
}
}
}
}
(!$fc) ? $self->test_result(1) : $self->test_result(0);
}
sub compare_fasta {
my($self) = @_;
$self->test_header('Comparing fasta output');
if($self->read_and_return_string("../test_data/$self->{program}.fasta")
ne $self->read_and_return_string("./c2ptest")){
print "Output from test_data/$self->{program} does not match output in file c2ptest\n";
$self->test_result(0);
return;
}
$self->test_result(1);
}
sub compare_qual {
my($self) = @_;
$self->test_header('Comparing quality output');
if($self->read_and_return_string("../test_data/$self->{program}.qual")
ne $self->read_and_return_string("./c2ptest.qual")){
print "Output from test_data/$self->{program} does not match output in file c2ptest\n";
$self->test_result(0);
return;
}
$self->test_result(1);
}
sub read_and_return_string {
my($self,$file) = @_;
local $/ = undef;
open(FILE,"$file") or return $file;
my $retstr = <FILE>;
$retstr =~ s/[\n|\s+]//g;
close(FILE);
return $retstr;
}
1;
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