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#include <ctype.h>
#include <fcntl.h>
#include <string.h>
#include <sys/stat.h>
#include <gclib/GBase.h>
#include <gclib/GArgs.h>
#include <gclib/GHash.hh>
#include <gclib/gcdb.h>
#ifdef ENABLE_COMPRESSION
#include "gcdbz.h"
#endif
#ifdef __WIN32__
#define VERSION "cdbfasta version 1.00 win32"
#else
#define VERSION "cdbfasta version 1.00"
#endif
#define USAGE "Usage:\n\
cdbfasta <fastafile> [-o <index_file>] [-r <record_delimiter>]\n\
[-z <compressed_db>] [-i] [-m|-n <numkeys>|-f<LIST>]|-c|-C]\n\
[-w <stopwords_list>] [-s <stripendchars>] [{-Q|-G}] [-v]\n\
\n\
Creates an index file for records from a multi-fasta file.\n\
By default (without -m/-n/-c/-C option), only the first \n\
space-delimited token from the defline is used as a key.\n\
\n\
<fastafile> is the multi-fasta file to index; \n\
-o the index file will be named <index_file>; if not given,\n\
the index filename is database name plus the suffix '.cidx'\n\
-r <record_delimiter> a string of characters at the beginning of line\n\
marking the start of a record (default: '>')\n\
-Q treat input as fastq format, i.e. with '@' as record delimiter\n\
and with records expected to have at least 4 lines\n\
-z database is compressed into the file <compressed_db>\n\
before indexing (<fastafile> can be \"-\" or \"stdin\" \n\
in order to get the input records from stdin)\n\
-s strip extraneous characters from *around* the space delimited\n\
tokens, for the multikey options below (-m,-n,-f);\n\
Default <stripendchars> set is: '\",`.(){}/[]!:;~|><+-\n\
-m (\"multi-key\" option) create hash entries pointing to \n\
the same record for all tokens found in\n\
the defline\n\
-n <numkeys> same as -m, but only takes the first <numkeys>\n\
tokens from the defline; when used with -a option (see below),\n\
only collects the first <numkeys> accessions from each defline\n\
-f indexes *space* delimited tokens (fields) in the defline as given\n\
by LIST of fields or fields ranges (the same syntax as UNIX 'cut')\n\
-w <stopwordslist> exclude from indexing all the words found\n\
in the file <stopwordslist> (for options -m, -n and -k)\n\
-i do case insensitive indexing (i.e. create additional keys for \n\
all-lowercase tokens used for indexing from the defline \n\
-c for deflines in the format: db1|accession1|db2|accession2|...,\n\
only the first db-accession pair ('db1|accession1') is taken as key\n\
-C like -c, but also subsequent db|accession constructs are indexed,\n\
along with the full (default) token; additionally,\n\
all nrdb concatenated accessions found in the defline \n\
are parsed and stored (assuming 0x01 or '^|^' as separators)\n\
-a accession mode: like -C but indexes only the 'accession' part for all\n\
'db|accession' constructs found, plus the default first tokens\n\
-A like -a and -C together (both accessions and 'db|accession'\n\
constructs are used as keys\n\
-D index each pipe ('|') delimited token found in the record identifier\n\
(e.g. >key1|key2|key3|.. )\n\
-d same as -D but using a custom key delimiter <kdelim> instead of the pipe\n\
character '|'\n\
-G FASTA records are treated as large genomic sequences (e.g. full \n\
chromosomes/contigs) and their formatting is checked for suitability\n\
for fast range queries (i.e. uniform line length within each record)\n\
-v show program version and exit\n"
/*
*/
#define ERR_TOOMANYFIELDS "Error: too many fields for -f option\n"
//16K initial defline buffer
#define KBUFSIZE 0x4000
#ifndef O_BINARY
#define O_BINARY 0x0000
#endif
#define MAX_KEYLEN 1024
//64K input buffer
#define GREADBUF_SIZE 0x010000
typedef void (*addFuncType)(char*, off_t, uint32);
char ftmp[365];
char fztmp[365];
char record_marker[127]; //record delimiter
int record_marker_len=1;
int num_recs;
int num_keys;
off_t last_cdbfpos=0;
int compact_plus; //shortcut key and
bool acc_mode=false;
bool acc_only=false;
bool do_compress=false; // compression used
bool fastq=false;
bool gFastaSeq=false;
char keyDelim=0;
FILE* zf=NULL; //compressed file handle
//store just offset and record length
const char* defWordJunk="'\",`.(){}/[]!:;~|><+-";
char* wordJunk=NULL;
bool caseInsensitive=false; //case insensitive storage
bool useStopWords=false;
unsigned int numFields=0;
// we have fields[numFields-1]=MAX_UINT as defined in gcdb.h --
// as an indicator of taking every single token in the defline,
// or for open ended ranges (e.g. -f5- )
unsigned int fields[255]; //array of numFields field indices (1-based)
GHash<int> stopList;
//static int datalen=sizeof(uint32)+sizeof(off_t);
char lastKey[MAX_KEYLEN]; //keep a copy of the last valid written key
GCdbWrite* cdbidx;
addFuncType addKeyFunc;
#define ERR_W_DBSTAT "Error writing the database statististics!\n"
void die_write(const char* fname) {
GError("Error: cdbhash was unable to write into file %s\n",
fname);
}
void die_read(const char* infile) {
GError("Error: cdbhash was unable to read the input file %s\n",infile);
}
void die_readformat(const char* infile) {
GError("Error: bad format for file %s; is it a fastA file?\n",
infile);
}
void die_gseqformat(const char* seqname) {
GError("Error: invalid FASTA sequence format for %s (not uniform line length)\n",
seqname);
}
void die_fastqformat(const char* seqid, int seqlen, int qvlen) {
GError("Error: invalid FASTQ sequence format for %s (seqlen=%d, qvlen=%d)\n",
seqid);
}
bool add_cdbkey(char* key, off_t fpos, uint32 reclen, int16_t linelen=0, byte elen=0) {
unsigned int klen=strlen(key);
if (fpos==last_cdbfpos && strcmp(key, lastKey)==0) return true;
if (klen<1) {
/* GMessage("Warning: zero length key found following key '%s'\n",
lastKey);
*/
return false;
}
//------------ adding record -----------------
num_keys++;
strncpy(lastKey, key, MAX_KEYLEN-1);
lastKey[MAX_KEYLEN-1]='\0';
if ((uint64)fpos>(uint64)MAX_UINT) { //64 bit file offset
uint64 v= (uint64) fpos; //needed for Solaris' off_t issues with gcc/32
/*
if (linelen>0) {
//record with line len
CIdxSeqData recdata;
recdata.fpos=gcvt_offt(&v);
recdata.reclen=gcvt_uint(&reclen);
recdata.linelen=gcvt_int16(&linelen);
recdata.elen=elen;
if (cdbidx->addrec(key,klen,(char*)&recdata,IdxSeqDataSIZE)==-1)
GError("Error adding cdb record with key '%s'\n",key);
}
else { //plain record */
CIdxData recdata;
recdata.fpos=gcvt_offt(&v);
recdata.reclen=gcvt_uint(&reclen);
if (cdbidx->addrec(key,klen,(char*)&recdata,IdxDataSIZE)==-1)
GError("Error adding cdb record with key '%s'\n",key);
//}
}
else {//32 bit file offset is enough
/*
if (linelen>0) {
CIdxSeqData32 recdata;
uint32 v=(uint32) fpos;
recdata.fpos=gcvt_uint(&v);
recdata.reclen=gcvt_uint(&reclen);
recdata.linelen=gcvt_int16(&linelen);
recdata.elen=elen;
if (cdbidx->addrec(key,klen,(char*)&recdata,IdxDataSIZE32)==-1)
GError("Error adding cdb record with key '%s'\n",key);
}
else { */
CIdxData32 recdata;
uint32 v=(uint32) fpos;
recdata.fpos=gcvt_uint(&v);
recdata.reclen=gcvt_uint(&reclen);
if (cdbidx->addrec(key,klen,(char*)&recdata, IdxDataSIZE32)==-1)
GError("Error adding cdb record with key '%s'\n",key);
//}
}
last_cdbfpos=fpos;
return true;
}
//default indexing: key directly passed --
// as the first space delimited token
void addKey(char* key, off_t fpos,
uint32 reclen) {
num_recs++;
add_cdbkey(key, fpos, reclen);
if (caseInsensitive) {
char* lckey=loCase(key);
if (strcmp(lckey, key)!=0)
add_cdbkey(lckey, fpos, reclen);
GFREE(lckey);
}
}
//the whole defline is passed
void addKeyMulti(char* defline,
off_t fpos, uint32 reclen) {
char* p=defline;
unsigned int fieldno=0;
char* pn;
num_recs++;
bool stillParsing=true;
unsigned int fidx=0; //index in fields[] array
while (stillParsing) {
while ((*p)==' ' || (*p)=='\t') p++;
if (*p == '\0') break;
//skip any extraneous characters at the beginning of the token
while (chrInStr(*p, wordJunk)) p++;
//skip any padding spaces or other extraneous characters
//at the beginning of the word
if (*p == '\0') break;
pn=p;
while (*pn!='\0' && !isspace(*pn)) pn++;
//found next space or end of string
fieldno++;
while (fields[fidx]<fieldno && fidx<numFields-1) fidx++;
//GMessage("> p=>'%s' [%d, %d, %d] (next='%s')\n",p, numFields,
// fieldno, fields[numFields-1], pn+1);
stillParsing = (((*pn)!='\0') && (fieldno+1<=fields[numFields-1]));
char* pend = pn-1; //pend is on the last non-space in the current token
//--strip the ending junk, if any
while (chrInStr(*pend, wordJunk) && pend>p) pend--;
if (pend<pn-1) *(pend+1)='\0';
else *pn='\0';
if (strlen(p)>0) {
if (fields[fidx]==MAX_UINT || fields[fidx]==fieldno) {
if (useStopWords && stopList.hasKey(p)) {
p=pn+1;
continue;
}
//--- store this key with the same current record data:
add_cdbkey(p, fpos, reclen);
//---storage code ends here
if (caseInsensitive) {
char* lcp=loCase(p);
if (strcmp(lcp,p)!=0)
add_cdbkey(lcp, fpos, reclen);
GFREE(lcp);
}
}
//if (isEnd) break; //if all the token were stored
}
p=pn+1;//p is now on the next token's start
} //token parsing loop
}
int qcmpInt(const void *p1, const void *p2) {
//int n1=*((int*)p1);
//int n2=*((int*)p2);
const unsigned int *a = (unsigned int *) p1;
const unsigned int *b = (unsigned int *) p2;
if (*a < *b) return -1;
else if (*a > *b) return 1;
else return 0;
}
char* parse_dbacc(char* pstart, char*& end_acc, char*& accst) {
if (pstart==NULL || *pstart==0) return NULL;
bool hasDigits=false;
char* pend=pstart;
end_acc=NULL; //end of first accession
accst=NULL;
while (*pstart=='|') pstart++;
for(char* p=pstart;;p++) {
if (hasDigits==false && *p>='0' && *p<='9') hasDigits=true;
/* if (*p==0) { //end of seq_name
pend=p; //doesn't matter if it's accession or "db"-like
break;
}*/
if (*p=='|' || *p==0) {
int curlen=p-pstart;
if (*p==0 || (hasDigits && curlen>3) || curlen>7 || accst!=NULL) {//previous token was an accession
pend=p; //advance pend
if (end_acc==NULL) end_acc=p;
if (accst==NULL) accst=pstart;
break;
}
else { //first pipe char or no digits
accst=p+1;
}
hasDigits=false;//reset flag
} // | separator
}
if (pend!=pstart) return pend;
else return NULL;
}
char* nextKeyDelim(char* pstart, char* pmax) {
if (pstart==NULL || pstart>=pmax) return NULL;
char* p=pstart;
while (*p!=keyDelim && p!=pmax && *p!=0) p++;
return p;
}
char* endSpToken(char* str) {
if (str==NULL) return NULL;
char* p=str;
//while (*p!=' ' && *p!='\t' && *p!='\v' && *p!=0) p++;
while (!isspace(*p) && *p!=0) p++;
*p=0;
return p;
}
#define NRDB_CHARSEP "\1\2\3\4"
#define NRDB_STRSEP "^|^"
//nrdbp is positioned at the end of current nrdb concatenated
//defline, or NULL if there is no more
inline void NRDB_Rec(char* &nrdbp, char* defline) {
nrdbp=strpbrk(defline, NRDB_CHARSEP);
if (nrdbp==NULL) {
nrdbp=strstr(defline, NRDB_STRSEP);
if (nrdbp!=NULL) {
*nrdbp='\0';
nrdbp+=2;
}
}
else *nrdbp='\0';
}
//-c/-C/-a/-A indexing: key up to the first space or second '|'
//receives the full defline
void addKeyCompact(char* defline,
off_t fpos, uint32 reclen) {
//we got the first token found on the defline
num_recs++;
char* nrdb_end;
//breaks defline at the next nrdb concatenation point
NRDB_Rec(nrdb_end, defline);
//isolate the first token in this nrdb concatenation
char* token_end=endSpToken(defline); //this puts 0 at the first space encountered
if (!compact_plus) { //shortcut key
//only the first db|accession construct will be indexed, if found
char* end_acc1=NULL; //end of first accession
char* acc1st=NULL;
char* dbacc_end=parse_dbacc(defline, end_acc1, acc1st);
if (end_acc1!=NULL) { //has acceptable shortcut
*end_acc1=0;
add_cdbkey(defline, fpos, reclen);
return;
}
if (dbacc_end!=NULL) {
*dbacc_end=0;
add_cdbkey(defline, fpos, reclen);
return;
}
//store this whole non-space token as key:
add_cdbkey(defline, fpos, reclen);
return;
}
//from now on only -C/-a/-A treatment:
int max_accs=255;
if (numFields>0) max_accs=numFields;
for(;;) {
//defline is on the first token
if (strlen(defline)>0) //add whole non-space token as the "full key"
add_cdbkey(defline, fpos, reclen);
//add the db|accession constructs as keys
char* dbacc_start=defline;
char* firstacc_end=NULL;
char* accst=NULL;
char* dbacc_end=parse_dbacc(dbacc_start, firstacc_end, accst);
int acc_keyed=0;
while (dbacc_end!=NULL) {
if (firstacc_end!=NULL && firstacc_end<dbacc_end) {
char c=*firstacc_end;
*firstacc_end=0;
if (!acc_only)
add_cdbkey(dbacc_start, fpos, reclen);
if (acc_mode && accst && acc_keyed<max_accs) {
add_cdbkey(accst, fpos, reclen);
++acc_keyed;
}
*firstacc_end=c;
}
if (dbacc_start==defline && dbacc_end==token_end) {
if (acc_mode && accst!=NULL && accst!=dbacc_start)
add_cdbkey(accst, fpos, reclen);
break; //the whole seq_name was only one db entry
}
*dbacc_end=0; //end key here
if (!acc_only)
add_cdbkey(dbacc_start, fpos, reclen);
if (acc_mode && accst && acc_keyed<max_accs) {
add_cdbkey(accst, fpos, reclen);
++acc_keyed;
}
if (dbacc_end==token_end)
break; //reached the end of this whole seq_name (non-space token)
dbacc_start=dbacc_end+1;
firstacc_end=NULL;
dbacc_end=parse_dbacc(dbacc_start, firstacc_end, accst);
} //parse inside a defline
// -- get to next concatenated defline, if any:
if (compact_plus && nrdb_end!=NULL) {
defline=nrdb_end+1; //look for the next nrdb concatenation
NRDB_Rec(nrdb_end, defline);
//isolate the first token in this nrdb record
token_end=endSpToken(defline);
}
else break;
} //for
}
void addKeyDelim(char* defline,
off_t fpos, uint32 reclen) {
//we got the first token found on the defline
num_recs++;
char* nrdb_end;
//breaks defline at the next nrdb concatenation point
NRDB_Rec(nrdb_end, defline);
//isolate the first space-delimited token in this nrdb concatenation
char* token_end=endSpToken(defline);
for(;;) {
//defline is on the first token
if (strlen(defline)==0) break;
//add the db|accession constructs as keys
char* k_start=defline;
char* k_end=NULL;
while ((k_end=nextKeyDelim(k_start, token_end))!=NULL) {
*k_end=0;
add_cdbkey(k_start, fpos, reclen);
k_start=k_end+1;
}
// -- get to next concatenated defline, if any:
if (nrdb_end!=NULL) {
defline=nrdb_end+1; //look for the next nrdb concatenation
NRDB_Rec(nrdb_end, defline);
//isolate the first space-delimited token in this nrdb record
token_end=endSpToken(defline);
}
else break;
} //for
}
int readWords(FILE* f, GHash<int>& xhash) {
int c;
int count=0;
char name[256];
int len=0;
while ((c=getc(f))!=EOF) {
if (isspace(c) || c==',' || c==';') {
if (len>0) {
name[len]='\0';
xhash.Add(name, new int(1));
count++;
len=0;
}
continue;
}
//a non-space
name[len]=(char) c;
len++;
if (len>255) {
name[len-1]='\0';
GError("Error reading words file: token too long ('%s') !\n",name);
}
}
if (len>0) {
name[len]='\0';
xhash.Add(name, new int(1));
count++;
}
return count;
}
//========================== MAIN ===============================
int main(int argc, char **argv) {
FILE* f_read=NULL;
off_t fdbsize;
int ch;
char* zfilename;
char* fname;
char* marker; //record marker
int maxkeys=0;
int multikey=0;
record_marker[0]='>';
record_marker[1]=0;
GArgs args(argc, argv, "icvDQCaAmn:o:r:z:w:f:s:d:");
int e=args.isError();
if (e>0)
GError("%s Invalid argument: %s\n", USAGE, argv[e] );
if (args.getOpt('v')!=NULL) {
printf("%s\n",VERSION);
return 0;
}
fastq = (args.getOpt('Q')!=NULL);
gFastaSeq=(args.getOpt('G')!=NULL);
if (fastq && gFastaSeq)
GError("Error: options -Q and -G are mutually exclusive.\n");
if (args.getOpt('D')) keyDelim='|';
else if (args.getOpt('d')!=NULL) keyDelim=args.getOpt('d')[0];
multikey = (args.getOpt('m')!=NULL);
if (multikey) {
fields[numFields]=1;
numFields++;
fields[numFields]=MAX_UINT;
numFields++;
}
caseInsensitive = (args.getOpt('i')!=NULL);
acc_only=(args.getOpt('a')!=NULL);
acc_mode=(acc_only || args.getOpt('A')!=NULL);
compact_plus=(args.getOpt('C')!=NULL || acc_mode);
wordJunk = (char *)args.getOpt('s');
if (wordJunk==NULL) wordJunk=(char*)defWordJunk;
int compact=(args.getOpt('c')!=NULL || compact_plus);
if ((compact && multikey) || (multikey && keyDelim)) {
GError("%s Error: invalid flag combination.\n", USAGE);
}
char* argfields = (char*)args.getOpt('f');
char* s = (char*)args.getOpt('n');
if (s!=NULL) {
maxkeys = atoi(s);
if (maxkeys>1 && (multikey || keyDelim || argfields!=NULL))
GError("%s Error: invalid options (-m, -c/C, -n, -D/-d and -f are exclusive)\n", USAGE);
numFields=maxkeys;
if (numFields<1) GError("Error: invalid -n option (must be a 1..255 value)\n");
if (numFields>254) GError(ERR_TOOMANYFIELDS);
if (!acc_only) {
multikey=1;
for (unsigned int i=1;i<=numFields;i++)
fields[i-1]=i;
}
}
if (argfields!=NULL) { //parse all the field #s
if (multikey || keyDelim || compact)
GError("%s Error: invalid options (-m, -c/C, -n, -D/-d and -f are exclusive)\n", USAGE);
char* pbrk;
int prevnum=0;
char prevsep='\0';
numFields=0;
char sep;
char *p=argfields;
do {
pbrk=strpbrk(p,",-");
if (pbrk==NULL) {
sep='\0';
pbrk=p+strlen(p);
if (prevsep == '-' && *p=='\0' && prevnum>0) {
//open ended range -- ending with '-'
fields[numFields]=prevnum;
numFields++;
if (numFields>253) GError(ERR_TOOMANYFIELDS);
fields[numFields]=MAX_UINT;
numFields++;
//GMessage("--- stored %d, %d\n",prevnum, MAX_UINT);
break;
}// ending with '-'
} // '\0'
else { sep=*pbrk; *pbrk = '\0'; }
int num = atoi(p);
if (num<=0 || num>254 )
GError("%s Error: invalid syntax for -f option.\n", USAGE);
if (prevsep == '-') { //store a range
for (int i=prevnum;i<=num;i++) {
fields[numFields]=i;
numFields++;
if (numFields>254) GError(ERR_TOOMANYFIELDS);
}
}
else if (sep!='-') {
fields[numFields]=num;
numFields++;
if (numFields>254) GError(ERR_TOOMANYFIELDS);
}
prevsep=sep;
prevnum=num;
p=pbrk+1;
} while (sep != '\0'); //range parsing loop
if (numFields<=0 || numFields>254 )
GError("%s Error at parsing -f option.\n", USAGE);
//GMessage("[%d] Fields parsed (%d values):\n", sizeof(fields[0]), numFields);
qsort(fields, numFields, sizeof(fields[0]), &qcmpInt);
multikey=1;
/*-- --------debug:
for (unsigned int i=0;i<numFields-1;i++) {
GMessage("%d,", fields[i]);
}
GMessage("%d\n",fields[numFields-1]);
exit(0); */
} //fields
if (fastq) {
record_marker[0]='@';
record_marker_len=1;
}
if (args.getOpt('r')!=NULL) {//non-FASTA record delimiter?
if (fastq) {
GMessage("Option -r ignored because -Q was given (-Q sets delimiter to '@')\n");
}
else {
marker=(char*)args.getOpt('r'); //
int v=0;
if (strlen(marker)>126)
GError("Error: the specified record delimiter is too long. "
"Maximum accepted is 126\n");
//special case: hex (0xXX) and octal codes (\XXX) are accepted, only if by themselves
if (strlen(marker)==4 && (marker[0]=='\\' || (marker[0]=='0' && toupper(marker[1])=='X') )) {
if (marker[0]=='\\') {
marker++;
v=strtol(marker, NULL, 8);
}
else v=strtol(marker, NULL, 16);
if (v==0 || v>255)
GError("Invalid record delimiter: should be only one character,\n"
"'\\NNN' (octal value), '0xNN' (hexadecimal value)");
record_marker[0]=v;
record_marker_len=1;
}
else {
strcpy(record_marker, marker);
record_marker_len=strlen(record_marker);
}
}
}
char* stopwords=(char*)args.getOpt('w'); //stop words filename given?
if (stopwords!=NULL) {
FILE* fstopwords=NULL;
if ((fstopwords=fopen(stopwords, "r"))==NULL)
GError("Cannot open stop words file '%s'!\n", stopwords);
int c=readWords(fstopwords, stopList);
GMessage("Loaded %d stop words.\n", c);
fclose(fstopwords);
useStopWords=(c>0);
}
if ((zfilename=(char*)args.getOpt('z')) !=NULL) {
do_compress=true;
#ifndef ENABLE_COMPRESSION
GError("Error: compression requested but not enabled when cdbfasta was compiled\n");
#endif
strcpy(fztmp,zfilename);
strcat(fztmp,"_ztmp");
zf=fopen(fztmp,"wb");
if (zf==NULL)
GError("Error creating file '%s'\n'", fztmp);
}
char* outfile=(char*) args.getOpt('o');
int numfiles = args.startNonOpt();
if (numfiles==0)
GError("%sError: no fasta file given.\n", USAGE);
fname=(char*) args.nextNonOpt(); //first fasta file given
if (do_compress) { //-------- compression case -------------------
if (strcmp(fname, "-")==0 || strcmp(fname, "stdin")==0)
f_read=stdin;
else f_read= fopen(fname, "rb");
if (f_read == NULL) die_read(fname);
fname=zfilename; //forget the input file name, keep the output
}
else {//
int fdread= open(fname, O_RDONLY|O_BINARY);
if (fdread == -1) die_read(fname);
struct stat dbstat;
fstat(fdread, &dbstat);
fdbsize=dbstat.st_size;
close(fdread);
f_read= fopen(fname, "rb");
if (f_read == NULL) die_read(fname);
}
char idxfile[365];
if (outfile==NULL) {
if (do_compress) {
strcpy(ftmp, zfilename);
strcat(ftmp, ".cidx");
strcpy(idxfile, ftmp);
strcat(ftmp, "_tmp");
}
else {
strcpy(ftmp, fname);
strcat(ftmp, ".cidx");
strcpy(idxfile, ftmp);
strcat(ftmp, "_tmp");
}
//should add the process ID, time and user to make this unique?
}
else {
strcpy(ftmp, outfile);
strcpy(idxfile, outfile);
strcat(ftmp, "_tmp");
}
cdbidx=new GCdbWrite(ftmp); //test if this was successful?
if (keyDelim>0) {
addKeyFunc=&addKeyDelim;
}
else {
if (compact)
addKeyFunc=&addKeyCompact;
else if (multikey)
addKeyFunc = &addKeyMulti;
else addKeyFunc = &addKey;
}
off_t recpos=0;
off_t r=0;
unsigned int recsize=0;
char* key=NULL;
bool fullDefline=(multikey || compact_plus);
GReadBuf *readbuf = new GReadBuf(f_read, GREADBUF_SIZE);
if (do_compress) { //---------------- compression case -------------
if (fastq) GError("Error: sorry, compression is not supported with fastq format\n");
//TODO: use a better block compression/indexing scheme with virtual offsets, like bgzf
// -- this should take care of the fastq compression
#ifdef ENABLE_COMPRESSION
fdbsize=0;
GCdbz cdbz(zf); // zlib interface
recpos=cdbz.getZRecPos();
while ((key=cdbz.compress(readbuf,record_marker))!=NULL) {
recsize=cdbz.getZRecSize();
if (!fullDefline) {
//find first space after the record_marker and place a '\0' there
for (int i=record_marker_len; key[i]!='\0';i++) {
if (isspace(key[i])) { key[i]='\0';break; }
}
}
addKeyFunc(key, recpos, recsize);
recpos = cdbz.getZRecPos();
}
remove(zfilename);
cdbz.compress_end();
fclose(zf);
//determine the size of this file:
int ftmp= open(fztmp, O_RDONLY|O_BINARY);
if (ftmp == -1) die_read(fztmp);
struct stat dbstat;
fstat(ftmp, &dbstat);
fdbsize=dbstat.st_size;
//rename it to the intended file name
if (rename(fztmp,zfilename) != 0) {
GMessage("Error: unable to rename '%s' to '%s'\n",fztmp,zfilename);
perror("rename");
}
#else
GError("Error: this program was not compiled with compression enabled.\n");
#endif
} //compression requested
else { // not compressed -- plain (buffered) file access
bool defline=false;
bool seen_defline=false;
int kbufsize=KBUFSIZE;
if (fullDefline) { GMALLOC(key, KBUFSIZE); }//large defline storage buffer, just in case
else { GMALLOC(key, 1024); }
key[0]=0; //no keys have been parsed yet
int kidx=-1;
num_recs=0;
num_keys=0;
char prevch=0;
//first iteration -- for the beginning of file
if (readbuf->peekCmp(record_marker, record_marker_len)==0) {
//new record start found (defline)
recpos=readbuf->getPos(); //new record pos
defline=true; //we're in defline
seen_defline=true;
readbuf->skip(record_marker_len);
kidx=0;
}//new record start
int linecounter=0; //number of non-header lines for current record
int fq_recloc=(fastq) ? 0 : 4; //0=seq header line, 1=sequence string, 2=q-header line, 3=qvstring, 4=not fastq
int fq_lendata[4]={0,0,0,0}; //keep track of fastq seq len, qv len
int last_linelen=0; //length of last non-header line in a FASTA record
int first_linelen=0; //length of the first non-header line in a FASTA record
int last_eol_len=0;
bool mustbeLastLine=false;
bool wasEOL=false;
while ((ch=readbuf->getch())>0) {
bool isEOL=false;
if (ch=='\n' || ch=='\r') {
isEOL=true;
last_eol_len=1;
}
if (wasEOL && isEOL) {
//skipped double-char EoL (DOS or empty lines)
if (prevch=='\n' && ch=='\r') last_eol_len=2;
prevch=ch;
wasEOL=isEOL;
continue;
}
if (defline) {
// ===> in the header line (including its EOL)
if (isEOL) {
// --> end of header line
if (kidx>=0) { key[kidx]=0;kidx=-1; }
defline=false;
last_linelen=0;
first_linelen=0;
linecounter=0;
fq_recloc= (fastq) ? 0 : 4;
mustbeLastLine=false;
memset((void*)fq_lendata, 0, 4*sizeof(int));
prevch=ch;
wasEOL=isEOL;
continue;
}
else {
// --> within header, before EOL
if (kidx>=0) { //still parsing keys
if ((isspace(ch) || ch<31)
&& fullDefline==false) {
key[kidx]=0;
kidx=-1;
wasEOL=false;
prevch=ch;
continue;
}
key[kidx]=(char)ch;
kidx++;
if (kidx>=kbufsize) {
kbufsize+=KBUFSIZE;
GREALLOC(key, kbufsize);
}
} //still parsing keys
} // <-- before EoL
} // <=== in the header line
else {
// ===> not in header line (could be "sequence block", or before first defline
if (isEOL) {
// <-- at EoL in "sequence" block (record body)
if (seen_defline) {
linecounter++; //counting sequence lines
if (gFastaSeq && linecounter>1) {
if (last_linelen>first_linelen)
die_gseqformat(key);
if (last_linelen<first_linelen)
mustbeLastLine=true;
}
}
} // --> at EoL
else {
// <-- before EoL
// could be a new header, should we check?
if (wasEOL) {
// - start of a new line
bool newRecStart=false;
bool checkNewRec = (!fastq || fq_lendata[1]<=fq_lendata[3]);
if (checkNewRec && ch==record_marker[0]) {
newRecStart=(record_marker_len>1) ?
(readbuf->peekCmp(&record_marker[1], record_marker_len-1)==0) :
true;
if (newRecStart) {
// new record start (new header line coming up)
seen_defline=true;
recsize = readbuf->getPos()-recpos-1-last_eol_len; //previous recsize
if (recsize>(off_t)(record_marker_len+1) && key[0]!='\0') {
//add previous record
//TODO: validate gFastaSeq or fastq here?
if (fastq && fq_lendata[1]!=fq_lendata[3])
die_fastqformat(key, fq_lendata[1], fq_lendata[3]);
addKeyFunc(key, recpos, recsize);
}
recpos=readbuf->getPos()-1; //new record pos (after reading this EOL)
if (record_marker_len>1)
readbuf->skip(record_marker_len-1); //skip past the record marker
defline=true; //we'll be in the header line in the next iteration
linecounter=0;
mustbeLastLine=false;
kidx=0; //reset keys
prevch=record_marker[record_marker_len-1];
wasEOL=(prevch=='\n' || prevch=='\r');
continue;
} // new record start
} //checking for new record start (record_marker match)
if (!seen_defline) {
prevch=ch;
wasEOL=isEOL;
continue;
}
if (mustbeLastLine && !newRecStart)
die_gseqformat(key);
// start of a new line in the "sequence" block
last_linelen=0;
mustbeLastLine=false;
if (fq_recloc<4) {
//take care of multi-line fastq parsing
if (fq_recloc==1 && ch=='+') {
fq_recloc=2; //q-header line
}
else if (fq_recloc==0 || fq_recloc==2) fq_recloc++;
} //fastq parsing
} // - start of a new non-header line (wasEoL)
if (!seen_defline) {
prevch=ch;
wasEOL=isEOL;
continue;
}
if (fq_recloc<4) {
fq_lendata[fq_recloc]++;
}
last_linelen++;
if (linecounter==0) first_linelen++;
} // --> record body, before EoL
} // <=== not in header line
prevch=ch;
wasEOL=isEOL;
}//while getch
recsize=readbuf->getPos()-recpos;
if (recsize>0) {//add last record, if there
if (prevch=='\n' || prevch=='\r') recsize-=last_eol_len;
if (fullDefline && kidx>0) {//close the defline string
if (prevch!='\n' && prevch!='\r') kidx++;
key[kidx-1]='\0';
}
addKeyFunc(key, recpos, recsize);
linecounter=0;
//GMessage("adding key=%s\n",key);
}
delete readbuf;
}
if (f_read!=stdin) fclose(f_read);
if (cdbidx->finish() == -1) die_write("");
// === add some statistics at the end of the cdb index file!
r=lseek(cdbidx->getfd(), 0, SEEK_END);
cdbInfo info;
memcpy((void*)info.tag, (void*)"CDBX", 4);
info.idxflags=0;
if (multikey) info.idxflags |= CDBMSK_OPT_MULTI;
if (do_compress) {
info.idxflags |= CDBMSK_OPT_COMPRESS;
GMessage("Input data were compressed into file '%s'\n",fname);
}
if (compact) {
if (compact_plus)
info.idxflags |= CDBMSK_OPT_CADD;
else
info.idxflags |= CDBMSK_OPT_C;
}
if (gFastaSeq) {
info.idxflags |= CDBMSK_OPT_GSEQ;
}
info.num_records=gcvt_uint(&num_recs);
info.num_keys=gcvt_uint(&num_keys);
info.dbsize=gcvt_offt(&fdbsize);
info.idxflags=gcvt_uint(&info.idxflags);
int nlen=strlen(fname);
info.dbnamelen=gcvt_uint(&nlen);
r=write(cdbidx->getfd(), fname, nlen);
if (r!=nlen)
GError(ERR_W_DBSTAT);
r=write(cdbidx->getfd(), &info, cdbInfoSIZE);
if (r!=cdbInfoSIZE)
GError(ERR_W_DBSTAT);
delete cdbidx;
GFREE(key);
remove(idxfile);
if (rename(ftmp,idxfile) == -1)
GError("Error: unable to rename %s to %s",ftmp,idxfile);
GMessage("%d entries from file %s were indexed in file %s\n",
num_recs, fname, idxfile);
return 0;
}
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