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/*
* Copyright 2011, Ben Langmead <langmea@cs.jhu.edu>
*
* This file is part of Bowtie 2.
*
* Bowtie 2 is free software: you can redistribute it and/or modify
* it under the terms of the GNU General Public License as published by
* the Free Software Foundation, either version 3 of the License, or
* (at your option) any later version.
*
* Bowtie 2 is distributed in the hope that it will be useful,
* but WITHOUT ANY WARRANTY; without even the implied warranty of
* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
* GNU General Public License for more details.
*
* You should have received a copy of the GNU General Public License
* along with Bowtie 2. If not, see <http://www.gnu.org/licenses/>.
*/
#ifndef ALIGNER_SEED_POLICY_H_
#define ALIGNER_SEED_POLICY_H_
#include "scoring.h"
#include "simple_func.h"
#define DEFAULT_SEEDMMS 0
#define DEFAULT_SEEDLEN 22
#define DEFAULT_IVAL SIMPLE_FUNC_SQRT
#define DEFAULT_IVAL_A 1.15f
#define DEFAULT_IVAL_B 0.0f
#define DEFAULT_UNGAPPED_HITS 6
/**
* Encapsulates the set of all parameters that affect what the
* SeedAligner does with reads.
*/
class SeedAlignmentPolicy {
public:
/**
* Parse alignment policy when provided in this format:
* <lab>=<val>;<lab>=<val>;<lab>=<val>...
*
* And label=value possibilities are:
*
* Bonus for a match
* 
*
* MA=xx (default: MA=0, or MA=2 if local is set)
*
* xx = Each position where equal read and reference characters match up
* in the alignment contriubtes this amount to the total score.
*
* Penalty for a mismatch
* 
*
* MMP={CxxQRQ} (default: MMP=C6)
*
* Cxx = Each mismatch costs xx. If MMP=Cxx is specified, quality
* values are ignored when assessing penalities for mismatches.
* Q = Each mismatch incurs a penalty equal to the mismatched base's
* value.
* R = Each mismatch incurs a penalty equal to the mismatched base's
* rounded quality value. Qualities are rounded off to the
* nearest 10, and qualities greater than 30 are rounded to 30.
*
* Penalty for position with N (in either read or reference)
* 
*
* NP={CxxQRQ} (default: NP=C1)
*
* Cxx = Each alignment position with an N in either the read or the
* reference costs xx. If NP=Cxx is specified, quality values are
* ignored when assessing penalities for Ns.
* Q = Each alignment position with an N in either the read or the
* reference incurs a penalty equal to the read base's quality
* value.
* R = Each alignment position with an N in either the read or the
* reference incurs a penalty equal to the read base's rounded
* quality value. Qualities are rounded off to the nearest 10,
* and qualities greater than 30 are rounded to 30.
*
* Penalty for a read gap
* 
*
* RDG=xx,yy (default: RDG=5,3)
*
* xx = Read gap open penalty.
* yy = Read gap extension penalty.
*
* Total cost incurred by a read gap = xx + (yy * gap length)
*
* Penalty for a reference gap
* 
*
* RFG=xx,yy (default: RFG=5,3)
*
* xx = Reference gap open penalty.
* yy = Reference gap extension penalty.
*
* Total cost incurred by a reference gap = xx + (yy * gap length)
*
* Minimum score for valid alignment
* 
*
* MIN=xx,yy (defaults: MIN=0.6,0.6, or MIN=0.0,0.66 if local is set)
*
* xx,yy = For a read of length N, the total score must be at least
* xx + (read length * yy) for the alignment to be valid. The
* total score is the sum of all negative penalties (from
* mismatches and gaps) and all positive bonuses. The minimum
* can be negative (and is by default in global alignment mode).
*
* N ceiling
* 
*
* NCEIL=xx,yy (default: NCEIL=0.0,0.15)
*
* xx,yy = For a read of length N, the number of alignment
* positions with an N in either the read or the
* reference cannot exceed
* ceiling = xx + (read length * yy). If the ceiling is
* exceeded, the alignment is considered invalid.
*
* Seeds
* 
*
* SEED=mm,len,ival (default: SEED=0,22)
*
* mm = Maximum number of mismatches allowed within a seed.
* Must be >= 0 and <= 2. Note that 2mismatch mode is
* not fully sensitive; i.e. some 2mismatch seed
* alignments may be missed.
* len = Length of seed.
* ival = Interval between seeds. If not specified, seed
* interval is determined by IVAL.
*
* Seed interval
* 
*
* IVAL={LSC},xx,yy (default: IVAL=S,1.0,0.0)
*
* L = let interval between seeds be a linear function of the
* read length. xx and yy are the constant and linear
* coefficients respectively. In other words, the interval
* equals a * len + b, where len is the read length.
* Intervals less than 1 are rounded up to 1.
* S = let interval between seeds be a function of the sqaure
* root of the read length. xx and yy are the
* coefficients. In other words, the interval equals
* a * sqrt(len) + b, where len is the read length.
* Intervals less than 1 are rounded up to 1.
* C = Like S but uses cube root of length instead of square
* root.
*
* Example 1:
*
* SEED=1,10,5 and read sequence is TGCTATCGTACGATCGTAC:
*
* The following seeds are extracted from the forward
* representation of the read and aligned to the reference
* allowing up to 1 mismatch:
*
* Read: TGCTATCGTACGATCGTACA
*
* Seed 1+: TGCTATCGTA
* Seed 2+: TCGTACGATC
* Seed 3+: CGATCGTACA
*
* ...and the following are extracted from the reversecomplement
* representation of the read and align to the reference allowing
* up to 1 mismatch:
*
* Seed 1: TACGATAGCA
* Seed 2: GATCGTACGA
* Seed 3: TGTACGATCG
*
* Example 2:
*
* SEED=1,20,20 and read sequence is TGCTATCGTACGATC. The seed
* length is 20 but the read is only 15 characters long. In this
* case, Bowtie2 automatically shrinks the seed length to be equal
* to the read length.
*
* Read: TGCTATCGTACGATC
*
* Seed 1+: TGCTATCGTACGATC
* Seed 1: GATCGTACGATAGCA
*
* Example 3:
*
* SEED=1,10,10 and read sequence is TGCTATCGTACGATC. Only one seed
* fits on the read; a second seed would overhang the end of the read
* by 5 positions. In this case, Bowtie2 extracts one seed.
*
* Read: TGCTATCGTACGATC
*
* Seed 1+: TGCTATCGTA
* Seed 1: TACGATAGCA
*/
static void parseString(
const std::string& s,
bool local,
bool noisyHpolymer,
bool ignoreQuals,
int& bonusMatchType,
int& bonusMatch,
int& penMmcType,
int& penMmcMax,
int& penMmcMin,
int& penNType,
int& penN,
int& penRdExConst,
int& penRfExConst,
int& penRdExLinear,
int& penRfExLinear,
SimpleFunc& costMin,
SimpleFunc& nCeil,
bool& nCatPair,
int& multiseedMms,
int& multiseedLen,
SimpleFunc& multiseedIval,
size_t& failStreak,
size_t& seedRounds,
SimpleFunc* penCanSplice = NULL,
SimpleFunc* penNoncanSplice = NULL,
SimpleFunc* penIntronLen = NULL);
};
#endif /*ndef ALIGNER_SEED_POLICY_H_*/
