1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229
|
/*
* Copyright 2011, Ben Langmead <langmea@cs.jhu.edu>
*
* This file is part of Bowtie 2.
*
* Bowtie 2 is free software: you can redistribute it and/or modify
* it under the terms of the GNU General Public License as published by
* the Free Software Foundation, either version 3 of the License, or
* (at your option) any later version.
*
* Bowtie 2 is distributed in the hope that it will be useful,
* but WITHOUT ANY WARRANTY; without even the implied warranty of
* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
* GNU General Public License for more details.
*
* You should have received a copy of the GNU General Public License
* along with Bowtie 2. If not, see <http://www.gnu.org/licenses/>.
*/
#ifndef EBWT_UTIL_H_
#define EBWT_UTIL_H_
#include <string>
#include <stdexcept>
#include <iostream>
#include <fstream>
#include <stdlib.h>
#include <string.h>
#include "bt2_idx.h"
///////////////////////////////////////////////////////////////////////
//
// Functions for printing and sanity-checking Ebwts
//
///////////////////////////////////////////////////////////////////////
/**
* Check that the ebwt array is internally consistent up to (and not
* including) the given side index by re-counting the chars and
* comparing against the embedded occ[] arrays.
*/
template <typename index_t>
void Ebwt<index_t>::sanityCheckUpToSide(int upToSide) const {
assert(isInMemory());
index_t occ[] = {0, 0, 0, 0};
ASSERT_ONLY(index_t occ_save[] = {0, 0, 0, 0});
index_t cur = 0; // byte pointer
const EbwtParams<index_t>& eh = this->_eh;
bool fw = false;
while(cur < (upToSide * eh._sideSz)) {
assert_leq(cur + eh._sideSz, eh._ebwtTotLen);
for(index_t i = 0; i < eh._sideBwtSz; i++) {
uint8_t by = this->ebwt()[cur + (fw ? i : eh._sideBwtSz-i-1)];
for(int j = 0; j < 4; j++) {
// Unpack from lowest to highest bit pair
int twoBit = unpack_2b_from_8b(by, fw ? j : 3-j);
occ[twoBit]++;
}
assert_eq(0, (occ[0] + occ[1] + occ[2] + occ[3]) % 4);
}
assert_eq(0, (occ[0] + occ[1] + occ[2] + occ[3]) % eh._sideBwtLen);
// Finished forward bucket; check saved [A], [C], [G] and [T]
// against the index_ts encoded here
ASSERT_ONLY(const index_t *uebwt = reinterpret_cast<const index_t*>(&ebwt()[cur + eh._sideBwtSz]));
ASSERT_ONLY(index_t as = uebwt[0]);
ASSERT_ONLY(index_t cs = uebwt[1]);
ASSERT_ONLY(index_t gs = uebwt[2]);
ASSERT_ONLY(index_t ts = uebwt[3]);
assert(as == occ_save[0] || as == occ_save[0]-1);
assert_eq(cs, occ_save[1]);
assert_eq(gs, occ_save[2]);
assert_eq(ts, occ_save[3]);
#ifndef NDEBUG
occ_save[0] = occ[0];
occ_save[1] = occ[1];
occ_save[2] = occ[2];
occ_save[3] = occ[3];
#endif
cur += eh._sideSz;
}
}
/**
* Sanity-check various pieces of the Ebwt
*/
template <typename index_t>
void Ebwt<index_t>::sanityCheckAll(int reverse) const {
const EbwtParams<index_t>& eh = this->_eh;
assert(isInMemory());
// Check ftab
for(index_t i = 1; i < eh._ftabLen; i++) {
assert_geq(this->ftabHi(i), this->ftabLo(i-1));
assert_geq(this->ftabLo(i), this->ftabHi(i-1));
assert_leq(this->ftabHi(i), eh._bwtLen+1);
}
assert_eq(this->ftabHi(eh._ftabLen-1), eh._bwtLen);
// Check offs
int seenLen = (eh._bwtLen + 31) >> ((index_t)5);
uint32_t *seen;
try {
seen = new uint32_t[seenLen]; // bitvector marking seen offsets
} catch(bad_alloc& e) {
cerr << "Out of memory allocating seen[] at " << __FILE__ << ":" << __LINE__ << endl;
throw e;
}
memset(seen, 0, 4 * seenLen);
index_t offsLen = eh._offsLen;
for(index_t i = 0; i < offsLen; i++) {
assert_lt(this->offs()[i], eh._bwtLen);
int w = this->offs()[i] >> 5;
int r = this->offs()[i] & 31;
assert_eq(0, (seen[w] >> r) & 1); // shouldn't have been seen before
seen[w] |= (1 << r);
}
delete[] seen;
// Check nPat
assert_gt(this->_nPat, 0);
// Check plen, flen
for(index_t i = 0; i < this->_nPat; i++) {
assert_geq(this->plen()[i], 0);
}
// Check rstarts
if(this->rstarts() != NULL) {
for(index_t i = 0; i < this->_nFrag-1; i++) {
assert_gt(this->rstarts()[(i+1)*3], this->rstarts()[i*3]);
if(reverse == REF_READ_REVERSE) {
assert(this->rstarts()[(i*3)+1] >= this->rstarts()[((i+1)*3)+1]);
} else {
assert(this->rstarts()[(i*3)+1] <= this->rstarts()[((i+1)*3)+1]);
}
}
}
// Check ebwt
sanityCheckUpToSide(eh._numSides);
VMSG_NL("Ebwt::sanityCheck passed");
}
/**
* Transform this Ebwt into the original string in linear time by using
* the LF mapping to walk backwards starting at the row correpsonding
* to the end of the string. The result is written to s. The Ebwt
* must be in memory.
*/
template <typename index_t>
void Ebwt<index_t>::restore(SString<char>& s) const {
assert(isInMemory());
s.resize(this->_eh._len);
index_t jumps = 0;
index_t i = this->_eh._len; // should point to final SA elt (starting with '$')
SideLocus<index_t> l(i, this->_eh, this->ebwt());
while(i != _zOff) {
assert_lt(jumps, this->_eh._len);
//if(_verbose) cout << "restore: i: " << i << endl;
// Not a marked row; go back a char in the original string
index_t newi = mapLF(l ASSERT_ONLY(, false));
assert_neq(newi, i);
s[this->_eh._len - jumps - 1] = rowL(l);
i = newi;
l.initFromRow(i, this->_eh, this->ebwt());
jumps++;
}
assert_eq(jumps, this->_eh._len);
}
/**
* Check that this Ebwt, when restored via restore(), matches up with
* the given array of reference sequences. For sanity checking.
*/
template <typename index_t>
void Ebwt<index_t>::checkOrigs(
const EList<SString<char> >& os,
bool color,
bool mirror) const
{
SString<char> rest;
restore(rest);
index_t restOff = 0;
size_t i = 0, j = 0;
if(mirror) {
// TODO: FIXME
return;
}
while(i < os.size()) {
size_t olen = os[i].length();
int lastorig = -1;
for(; j < olen; j++) {
size_t joff = j;
if(mirror) joff = olen - j - 1;
if((int)os[i][joff] == 4) {
// Skip over Ns
lastorig = -1;
if(!mirror) {
while(j < olen && (int)os[i][j] == 4) j++;
} else {
while(j < olen && (int)os[i][olen-j-1] == 4) j++;
}
j--;
continue;
}
if(lastorig == -1 && color) {
lastorig = os[i][joff];
continue;
}
if(color) {
assert_neq(-1, lastorig);
assert_eq(dinuc2color[(int)os[i][joff]][lastorig], rest[restOff]);
} else {
assert_eq(os[i][joff], rest[restOff]);
}
lastorig = (int)os[i][joff];
restOff++;
}
if(j == os[i].length()) {
// Moved to next sequence
i++;
j = 0;
} else {
// Just jumped over a gap
}
}
}
#endif /*EBWT_UTIL_H_*/
|