File: scaffold_graph.cpp

package info (click to toggle)
cufflinks 1.3.0-2
  • links: PTS, VCS
  • area: non-free
  • in suites: wheezy
  • size: 3,864 kB
  • sloc: cpp: 48,999; ansic: 12,297; sh: 3,381; python: 432; makefile: 209
file content (304 lines) | stat: -rw-r--r-- 7,969 bytes parent folder | download | duplicates (6)
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
251
252
253
254
255
256
257
258
259
260
261
262
263
264
265
266
267
268
269
270
271
272
273
274
275
276
277
278
279
280
281
282
283
284
285
286
287
288
289
290
291
292
293
294
295
296
297
298
299
300
301
302
303
304
/*
 *  scaffold_graph.cpp
 *  cufflinks
 *
 *  Created by Cole Trapnell on 6/2/10.
 *  Copyright 2010 Cole Trapnell. All rights reserved.
 *
 */

#include <vector>
#include "scaffold_graph.h"
#include "scaffolds.h"

#include <boost/graph/depth_first_search.hpp>
#include <boost/graph/visitors.hpp>

#ifndef NDEBUG
#include "transitive_reduction.h"
#endif

using namespace std;
using namespace boost;

struct HitBufBasket
{
	HitBufBasket(int coord, Scaffold* h, DAGNode d)
    : expiration_coord(coord), hit(h), node(d) {}
	int expiration_coord;
	Scaffold* hit;
	DAGNode node;
};



bool right_lt (const HitBufBasket& lhs, 
			   const HitBufBasket& rhs)
{
	return lhs.expiration_coord < rhs.expiration_coord;
}

struct Expired
{
	Expired(int ec) : expiration_coord(ec) {}
	bool operator()(const HitBufBasket& lhs)
	{
		return lhs.expiration_coord <= expiration_coord;
	}
	
	int expiration_coord;
};

enum ConnectState { UNKNOWN, CONNECT, DONT_CONNECT };

template <class CompatibilityMap, class ConnectMap,  class Tag>
struct connect_visitor
: public base_visitor<connect_visitor<CompatibilityMap, ConnectMap,  Tag> >
{
    typedef Tag event_filter;
    connect_visitor(CompatibilityMap compatibility, ConnectMap connect, DAGNode t) 
    : _connect(connect),_compatibility(compatibility), _target(t) { }
	
    template <class Vertex, class Graph>
    void operator()(Vertex u, const Graph& g)
	{
		typedef graph_traits<Graph> GraphTraits;
		
		typename GraphTraits::adjacency_iterator v, vend;
		
		if (_compatibility[u] == true)
		{
			for (tie(v,vend) = adjacent_vertices(u, g); v != vend; ++v)
			{
				if (_compatibility[*v])
				{
					//fprintf(stderr, "Avoiding a redundant edge from %d to %d\n", u, *v);
					_connect[u] = DONT_CONNECT;
					return;
				}
			}
			
			// If we get here, u is compatible with the target, but has no
			// compatible successors, so it's safe to add the edge after the DFS
			_connect[u] = CONNECT;
		}
		else
		{
			_connect[u] = DONT_CONNECT;
		}
		//put(_compat, v, compatible);
    }
	
    ConnectMap _connect;
	CompatibilityMap _compatibility;
	DAGNode _target;
};

template <class CompatibilityMap, class ConnectMap, class Tag>
connect_visitor<CompatibilityMap, ConnectMap, Tag>
record_connections(CompatibilityMap compatibility, 
				   ConnectMap connect, 
				   DAGNode target, 
				   Tag) 
{
    return connect_visitor<CompatibilityMap, ConnectMap, Tag> (compatibility, connect, target);
}


bool create_overlap_dag(vector<Scaffold>& hits,
                        DAG& bundle_dag)
{
    bundle_dag = DAG();
	vector<Scaffold>::iterator hi =  hits.begin();
	bool found_compatible_scaffolds = false;
	
	typedef list<HitBufBasket> HitBuf;
	HitBuf hit_buf;
	
	HitsForNodeMap hits_for_node = get(vertex_name, bundle_dag);
	
	while (hi != hits.end())
	{
		int new_left = hi->left();
		int new_right = hi->right();
        
        //fprintf(stderr, "Adding to hit buffer: [%d, %d)\n", new_left, new_right);
        
		HitBufBasket new_basket(new_right, &(*hi), add_vertex(bundle_dag));
		hits_for_node[new_basket.node] = new_basket.hit;
		
		HitBuf::iterator new_end = remove_if(hit_buf.begin(), 
											 hit_buf.end(), 
											 Expired(new_left));
		
		hit_buf.erase(new_end, hit_buf.end());
        
		// Now check the each hit in the buffer for compatibility with this
		// new one
		
		vector<const Scaffold*> containing_hits;
		
		boost::vector_property_map<bool> c(num_vertices(bundle_dag));
		boost::vector_property_map<ConnectState> connected(num_vertices(bundle_dag));
        
		for (HitBuf::iterator bi = hit_buf.begin();
			 bi != hit_buf.end();
			 ++bi)
			
		{
			const Scaffold& lhs = *(bi->hit);
			const Scaffold& rhs = *(new_basket.hit);
            
			assert (lhs.left() <= rhs.left());
			if (!lhs.contains(rhs))
			{
                //fprintf(stderr, "Checking [%d, %d) and [%d, %d)\n", lhs.left(), lhs.right(), rhs.left(), rhs.right());
				if (Scaffold::compatible(lhs, rhs))
				{
					c[bi->node] = true;
				}
			}
		}
		
		for (HitBuf::iterator bi = hit_buf.begin();
			 bi != hit_buf.end();
			 ++bi)
			
		{
			if (connected[bi->node] == UNKNOWN)
			{
				depth_first_search(bundle_dag,
								   root_vertex(bi->node).
								   visitor(make_dfs_visitor(make_pair(record_connections(c, connected, new_basket.node, on_finish_vertex()), null_visitor()))));
			}
		}
		
		for (HitBuf::iterator bi = hit_buf.begin();
			 bi != hit_buf.end();
			 ++bi)
		{
			if (connected[bi->node] == CONNECT)
			{ 
				add_edge(bi->node, new_basket.node, bundle_dag);
				found_compatible_scaffolds = true;
			}
		}
		
		hit_buf.push_back(new_basket);
		
		++hi;
	}
	
	vector<bool> has_parent(num_vertices(bundle_dag), false);
	vector<bool> has_child (num_vertices(bundle_dag), false);
	
	graph_traits < DAG >::vertex_iterator u, uend;
	for (tie(u, uend) = vertices(bundle_dag); u != uend; ++u)
	{
		graph_traits < DAG >::adjacency_iterator v, vend;
		for (tie(v,vend) = adjacent_vertices(*u, bundle_dag); v != vend; ++v)
		{
			DAGNode U = *u;
			DAGNode V = *v;
			has_parent[V] = true;
			has_child[U] = true;
		}
	}
	
#ifdef DEBUG
	set<const Scaffold*> introns;
#endif
	for (size_t i = 0; i < num_vertices(bundle_dag); ++i)
	{
		if (has_child[i])
			continue;
		const Scaffold* hit_i = hits_for_node[i];
        
		for (size_t j = 0; j < num_vertices(bundle_dag); ++j)
		{
			if (has_parent[j])
				continue;
			const Scaffold* hit_j = hits_for_node[j];
			if (hit_i->right() < hit_j->left() &&
				hit_j->left() - hit_i->right() < olap_radius)
			{
				add_edge(i, j, bundle_dag);
			}
		}
	}
    
#ifndef NDEBUG
    DAG tr;
    boost::vector_property_map<DAGNode> G_to_TR;
    property_map<DAG, vertex_index_t>::type w = get(vertex_index, bundle_dag);
    transitive_reduction(bundle_dag, 
                         tr, 
                         G_to_TR,
                         w);
    verbose_msg("dag has %lu edges, tr has %lu edges\n", num_edges(bundle_dag), num_edges(tr));
    
	//assert (num_edges(bundle_dag) == num_edges(tr));
#endif
    
	return found_compatible_scaffolds;
}

pair<DAGNode, DAGNode> add_terminal_nodes(DAG& bundle_dag)
{
    vector<char> has_parent(num_vertices(bundle_dag) + 2, false);
    vector<char> has_child (num_vertices(bundle_dag) + 2, false);
    
    graph_traits < DAG >::vertex_iterator u, uend;
    for (tie(u, uend) = vertices(bundle_dag); u != uend; ++u)
    {
        graph_traits < DAG >::adjacency_iterator v, vend;
        for (tie(v,vend) = adjacent_vertices(*u, bundle_dag); v != vend; ++v)
        {
            DAGNode U = *u;
            DAGNode V = *v;
            has_parent[V] = true;
            has_child[U] = true;
        }
    }
    
    DAGNode source = add_vertex(bundle_dag);
    DAGNode sink = add_vertex(bundle_dag);
    
    int num_attached_to_source = 0;
    int num_attached_to_sink = 0;
    
    for (size_t i = 0; i < num_vertices(bundle_dag); ++i)
    {
        if (!has_parent[i] && i != sink && i != source)
        {
            num_attached_to_source++;
            add_edge(source, i, bundle_dag);
        }
        if (!has_child[i] && i != source && i != sink)
        {
            num_attached_to_sink++;
            add_edge(i, sink, bundle_dag);
        }
    }
    
#if verbose_msg
    HitsForNodeMap hits_for_node = get(vertex_name, bundle_dag);
    DAG::vertex_iterator ki, ke;
	for (tie(ki, ke) = vertices(bundle_dag); ki != ke; ++ki)
	{
        if (edge(source, *ki, bundle_dag).second)
        {
            const Scaffold* pS = hits_for_node[*ki];
            fprintf(stderr, "%d-%d has edge from source\n", pS->left(), pS->right());
        }
        
        if (edge(*ki, sink, bundle_dag).second)
        {
            const Scaffold* pS = hits_for_node[*ki];
            fprintf(stderr, "%d-%d has edge to sink\n", pS->left(), pS->right());
        }
    }
    verbose_msg("%d source nodes, %d sink nodes\n", num_attached_to_source, num_attached_to_sink);
#endif
    return make_pair(source, sink);
}