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/*******************************************************************************************
*
* Split a .db into a set of sub-database blocks for use by the Dazzler:
* Divide the database <path>.db conceptually into a series of blocks referable to on the
* command line as <path>.1.db, <path>.2.db, ... If the -x option is set then all reads
* less than the given length are ignored, and if the -a option is not set then secondary
* reads from a given well are also ignored. The remaining reads are split amongst the
* blocks so that each block is of size -s * 1Mbp except for the last which necessarily
* contains a smaller residual. The default value for -s is 400Mbp because blocks of this
* size can be compared by our "overlapper" dalign in roughly 16Gb of memory. The blocks
* are very space efficient in that their sub-index of the master .idx is computed on the
* fly when loaded, and the .bps file of base pairs is shared with the master DB. Any
* tracks associated with the DB are also computed on the fly when loading a database block.
*
* Author: Gene Myers
* Date : September 2013
* Mod : New splitting definition to support incrementality, and new stub file format
* Date : April 2014
*
********************************************************************************************/
#include <stdlib.h>
#include <stdio.h>
#include <string.h>
#include <unistd.h>
#include "DB.h"
#ifdef HIDE_FILES
#define PATHSEP "/."
#else
#define PATHSEP "/"
#endif
static char *Usage = "[-aflm] [-x<int>] [-s<double(200.)>] <path:db|dam>";
static DAZZ_READ *reads;
static int MSORT(const void *l, const void *r)
{ int x = *((int *) l);
int y = *((int *) r);
return (reads[x].rlen - reads[y].rlen);
}
int main(int argc, char *argv[])
{ DAZZ_DB db, dbs;
int64 dbpos;
FILE *dbfile, *ixfile;
char *dbfile_name, *ixfile_name;
int status;
int nreads;
int medmax = 0;
int *msort = NULL;
int FORCE;
int ALL;
int CUTOFF;
int64 SIZE;
int SELECT;
{ int i, j, k;
int flags[128];
char *eptr;
float size;
ARG_INIT("DBsplit")
SELECT = -1;
CUTOFF = 0;
size = 200;
j = 1;
for (i = 1; i < argc; i++)
if (argv[i][0] == '-')
switch (argv[i][1])
{ default:
ARG_FLAGS("aflm")
break;
case 'x':
ARG_NON_NEGATIVE(CUTOFF,"Min read length cutoff")
break;
case 's':
ARG_REAL(size)
if (size <= 0.)
{ fprintf(stderr,"%s: Block size must be a positive number\n",Prog_Name);
exit (1);
}
break;
}
else
argv[j++] = argv[i];
argc = j;
SIZE = size*1000000ll;
ALL = flags['a'];
FORCE = flags['f'];
if (flags['l'])
{ SELECT = 0;
if (flags['m'])
{ fprintf(stderr,"%s: Cannot set both -l and -m, mutually exclusive\n",Prog_Name);
exit (1);
}
}
else if (flags['m'])
SELECT = 1;
if (argc != 2)
{ fprintf(stderr,"Usage: %s %s\n",Prog_Name,Usage);
fprintf(stderr,"\n");
fprintf(stderr," -s: Target size of blocks (in Mbp).\n");
fprintf(stderr," -x: Trimmed DB has reads >= this threshold.\n");
fprintf(stderr," -a: Trimmed DB contains all reads from a well (not just longest).\n");
fprintf(stderr," -f: Force the split to occur even if already split.\n");
fprintf(stderr,"\n");
fprintf(stderr," -l: Set primary read for a well to be the longest.\n");
fprintf(stderr," -m: Set primary read for a well to be the median.\n");
exit (1);
}
}
// Open db
status = Open_DB(argv[1],&db);
if (status < 0)
exit (1);
if (db.part > 0)
{ fprintf(stderr,"%s: Cannot be called on a block: %s\n",Prog_Name,argv[1]);
exit (1);
}
reads = db.reads;
nreads = db.ureads;
{ char *pwd, *root;
char buffer[2*MAX_NAME+100];
int nfiles;
int i;
pwd = PathTo(argv[1]);
if (status)
{ root = Root(argv[1],".dam");
dbfile_name = Strdup(Catenate(pwd,"/",root,".dam"),"Allocating db file name");
}
else
{ root = Root(argv[1],".db");
dbfile_name = Strdup(Catenate(pwd,"/",root,".db"),"Allocating db file name");
}
ixfile_name = Strdup(Catenate(pwd,PATHSEP,root,".idx"),"Allocating index file name");
dbfile = Fopen(dbfile_name,"r+");
ixfile = Fopen(ixfile_name,"r+");
if (dbfile_name == NULL || ixfile_name == NULL || dbfile == NULL || ixfile == NULL)
exit (1);
free(pwd);
free(root);
FSCANF(dbfile,DB_NFILE,&nfiles)
for (i = 0; i < nfiles; i++)
FGETS(buffer,2*MAX_NAME+100,dbfile)
FFREAD(&dbs,sizeof(DAZZ_DB),1,ixfile)
if (dbs.cutoff >= 0 && !FORCE)
{ printf("You are about to overwrite the current partition settings. This\n");
printf("will invalidate any tracks, overlaps, and other derivative files.\n");
printf("Are you sure you want to proceed? [Y/N] ");
fflush(stdout);
fgets(buffer,100,stdin);
if (index(buffer,'n') != NULL || index(buffer,'N') != NULL)
{ printf("Aborted\n");
fflush(stdout);
fclose(dbfile);
fclose(ixfile);
exit (1);
}
}
FTELLO(dbpos,dbfile)
FSEEKO(dbfile,dbpos,SEEK_SET)
FPRINTF(dbfile,DB_NBLOCK,0)
FPRINTF(dbfile,DB_PARAMS,SIZE,CUTOFF,ALL)
}
if (SELECT >= 0)
{ int i, j, k, h;
int off;
off = (~ DB_BEST);
for (i = 0; i < nreads; i = j)
{ j = i+1;
reads[i].flags &= off;
while ((reads[j].flags & DB_CCS) != 0)
reads[j++].flags &= off;
if (j-i <= 1)
{ reads[i].flags |= DB_BEST;
continue;
}
if (SELECT == 0)
{ int mlen;
mlen = reads[h = i].rlen;
for (k = i+1; k < j; k++)
if (mlen < reads[k].rlen)
mlen = reads[h = k].rlen;
}
else
{ if (j-i > medmax)
{ medmax = 1.2*(j-i) + 1000;
msort = (int *) Realloc(msort,sizeof(int)*medmax,"Allocating Median Array");
if (msort == NULL)
exit (1);
}
for (k = i; k < j; k++)
msort[k-i] = k;
qsort(msort,j-i,sizeof(int),MSORT);
h = msort[(j-i)/2];
}
reads[h].flags |= DB_BEST;
}
}
{ int64 totlen;
int nblock, ireads, treads, rlen, fno;
int i, upos, css;
css = 0;
nblock = 0;
totlen = 0;
ireads = 0;
treads = 0;
FPRINTF(dbfile,DB_BDATA,0,0)
if (ALL)
for (i = 0; i < nreads; i++)
{ rlen = reads[i].rlen;
if ((reads[i].flags & DB_CCS) == 0)
css = 0;
if (rlen >= CUTOFF)
{ if (css == 0 && totlen >= SIZE)
{ FPRINTF(dbfile,DB_BDATA,i,treads)
totlen = 0;
ireads = 0;
nblock += 1;
}
ireads += 1;
treads += 1;
totlen += rlen;
css = 1;
}
}
else
for (i = 0; i < nreads; i++)
{ rlen = reads[i].rlen;
if (rlen >= CUTOFF && (reads[i].flags & DB_BEST) != 0)
{ ireads += 1;
treads += 1;
totlen += rlen;
if (totlen >= SIZE)
{ FPRINTF(dbfile,DB_BDATA,i+1,treads)
totlen = 0;
ireads = 0;
nblock += 1;
}
}
}
if (ireads > 0)
{ FPRINTF(dbfile,DB_BDATA,nreads,treads)
nblock += 1;
}
fno = fileno(dbfile);
FTELLO(upos,dbfile)
if (ftruncate(fno,upos) < 0)
SYSTEM_WRITE_ERROR
FSEEKO(dbfile,dbpos,SEEK_SET)
FPRINTF(dbfile,DB_NBLOCK,nblock)
dbs.cutoff = CUTOFF;
if (ALL)
dbs.allarr |= DB_ALL;
else
dbs.allarr &= ~DB_ALL;
dbs.treads = treads;
FSEEKO(ixfile,0,SEEK_SET)
FFWRITE(&dbs,sizeof(DAZZ_DB),1,ixfile)
if (SELECT >= 0)
FFWRITE(reads,sizeof(DAZZ_READ),nreads,ixfile)
}
FCLOSE(ixfile)
FCLOSE(dbfile)
Close_DB(&db);
exit (0);
}
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