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#include <time.h>
#include <stdio.h>
#include <stdlib.h>
#include <math.h>
#include <string.h>
#include <ctype.h>
#include <time.h>
#include "struct.h"
#include "parameters.h"
extern void error(char *message);
extern void merror(char *msg1, char *msg2);
// io.c#
extern void print_diag(struct diag* aDiag);
// diag.c
//extern struct seq_part* create_seq_part(int num, struct seq* aSeq,
// long startpos);
extern struct diag* create_diag(struct seq_part* part1, struct seq_part* part2,
int dlength);
/**
*
* prob.c: Probability measurement (Creation of random sequences, diags,...)
*
* 2003-10-14 A.R.Subramanian
* (Initial)
*/
/**
* auxiliary method that fills random data into the sequence (see next func.)
*/
void fill_random_seq(struct seq* sq, struct scr_matrix *smatrix) {
long length = sq->length;
char *data = sq->data;
int *n2c = smatrix->num2char;
float slen = (float)smatrix->length;
int pos, rnd;
for(pos=0; pos<length;pos++) {
do {
rnd = (int)( (slen*random())/(RAND_MAX+1.0));
data[pos]=(char)(n2c[rnd]);
} while(n2c[rnd]=='X' || (n2c[rnd]<'A') || (n2c[rnd]>'Z'));
}
}
/**
* create random squence of the given length using the characters
* of the given score matrix
*
* The pointer returned (and the ones included in the struct)
* has to be deallocted explicitely from memory.
*/
struct seq* create_random_seq(struct scr_matrix *smatrix, int length) {
struct seq* sq = calloc(1, sizeof(struct seq));
sq->length=length;
sq->data = calloc(length, sizeof(char));
sq->name = calloc(strlen("random")+1, sizeof(char));
strcpy(sq->name, "random");
fill_random_seq(sq, smatrix);
return sq;
}
/**
* calculates the score distribution up to the given maximum diaglen
*
* The pointer returned and the included dist pointer has to be deallocted
* explicitely from memory.
*/
struct prob_dist* calc_score_dist(struct scr_matrix *smatrix, int mxdlen) {
long sm_max_scr = smatrix->max_score;
long maxdlen = mxdlen;
struct prob_dist *sdist = calloc(1, sizeof(struct prob_dist));
long **edist = (calloc(maxdlen+1, sizeof(long *)));
long **tdist = (calloc(maxdlen+1, sizeof(long *)));
long double **dist = (sdist->data=calloc(maxdlen+1, sizeof(long double *)));
if(sdist==NULL || dist==NULL || edist==NULL|| tdist==NULL) error("calc_score_dist(): Out of memory !");
sdist->max_dlen = maxdlen;
int *sdata = smatrix->data;
int *smdist = smatrix->dist;
sdist->smatrix = smatrix;
sdist->max_dlen = maxdlen;
int reduce = 3; // special characters '?', '#', '%', we don't account for
if(smatrix->char2num['X']>0) reduce++; // don't account for 'X'
long double square = (smatrix->length-reduce) * (smatrix->length-reduce);
long scr;
unsigned long i,j, scr2, mxscr, omxscr;
for(i=1;i<=maxdlen;i++) {
mxscr = i*sm_max_scr;
dist[i] = calloc(mxscr+1, sizeof(long double ));
edist[i] = calloc(mxscr+1, sizeof(long ));
tdist[i] = calloc(mxscr+1, sizeof(long ));
if(dist[i]==NULL) error("calc_score_dist(): Out of memory at iteration" );
if(i==1) {
for(j=0;j<=mxscr;j++) {
dist[i][j] = smdist[j]; //static, wie oft kommt ein score j in der scr-Matrix vor, nur fr Fragmentlnge 1, ansonsten initialisiere mit Null
edist[i][j]=0;
tdist[i][j]=0;
}
} else {
for(scr=0;scr<=mxscr;scr++) {
dist[i][scr]=0.0;
edist[i][scr]=0;
tdist[i][scr]=0;
omxscr = (i-1)*sm_max_scr;
for(j=0;j<=sm_max_scr;j++) {
if((scr-j)<=omxscr) dist[i][scr] += dist[1][j]*dist[i-1][scr-j]; // statisch wenn grer eins
}
}
}
}
struct seq *sq1, *sq2;
long max_experiment=550000, ex, found;
struct diag dg;
char *data1;
char *data2;
int *c2n = smatrix->char2num;
int a1, a2,pos,dpos1,dpos2;
long double experiment,oldprob,opf,opf2;
int dlen = 100;
// int maxdlen=100;
long double factor = (long double)dlen*dlen;
//long double expect;
for(i=1;i<=maxdlen;i++) {
// printf(" Diag of length %i\n", i);
mxscr = i*sm_max_scr;
//expect =0.0;
dlen = i;
factor = (long double)dlen*dlen;
for(scr=0;scr<=mxscr;scr++) {
//expect += ((long double)scr) * dist[i][scr]*pow(1.0/square,i);;
//printf(" max %i\n", mxscr);
for(scr2=scr+1;scr2<=mxscr;scr2++) {
dist[i][scr] += dist[i][scr2];
}
dist[i][scr] = dist[i][scr]*pow(1.0/square,i);
}
}
// EXPERIMENTS:
dg.seq_p1.sq = sq1;
dg.seq_p2.sq = sq2;
dg.length =2*maxdlen;
srandom((int)time(NULL));
sq1 = create_random_seq(smatrix, 2*maxdlen);
sq2 = create_random_seq(smatrix, 2*maxdlen);
//printf(" pre random\n");
// for(ex=0;(ex<max_experiment || found==0);ex++) {
char seenmax=0;
for(ex=0;(ex<max_experiment);ex++) {
if( (ex%1000)==0) fprintf(stderr," Experiment %li\n", ex);
fill_random_seq(sq1, smatrix);
fill_random_seq(sq2, smatrix);
data1 = sq1->data;
data2 = sq2->data;
for(dpos1=0;dpos1<=maxdlen;dpos1++) {
for(dpos2=0;dpos2<=maxdlen;dpos2++) {
dg.seq_p1.startpos = dpos1;//(int)( (100*random())/(RAND_MAX+1.0));
dg.seq_p2.startpos = dpos2; //(int)( (100*random())/(RAND_MAX+1.0));
dg.score = 0;
for(pos=0;pos<maxdlen;pos++) {
a1 = c2n[(int) data1[dg.seq_p1.startpos+pos]];
a2 = c2n[(int) data2[dg.seq_p2.startpos+pos]];
dg.score+=(long)sdata[smatrix->length*a1+a2];
if(dpos1<=pos+1 && dpos2<=pos+1) // t6
tdist[pos+1][dg.score]=1; // kann mehrmals dasselbe sein?? kann immer wieder berschrieben werden, warum?
}
}
}
for(i=1;i<=maxdlen;i++) {
//printf(" Diag of length %i\n", i);
mxscr = i*sm_max_scr;
seenmax =0;
for(scr=mxscr;scr>=0;scr--) {
if(!seenmax) {
if(tdist[i][scr]==1) {
edist[i][scr]=edist[i][scr]+1; //fr jedes Exp. maximalen score der jeweiligen Fragmentlnge hochzhlen
seenmax = 1;
}
}
tdist[i][scr]=0; // alles wieder auf Null setzen !
}
}
}
free(sq1);
free(sq2);
for(i=1;i<=maxdlen;i++) {
// printf(" Diag of length %i\n", i);
mxscr = i*sm_max_scr;
//expect =0.0;
dlen = i;
factor = (long double)(dlen+1)*(dlen+1); // t6
//factor = (long double)10201.0;
for(scr=0;scr<=mxscr;scr++) {
for(scr2=scr+1;scr2<=mxscr;scr2++) {
edist[i][scr] += edist[i][scr2]; // da in scr2 ja auch schon der scr1 beinhaltet ist; durch aufsummieren bekommen wir die Anzahl, wie viele Fragmente der Lnge i min. den score scr haben (oder mehr)
}
found = edist[i][scr]; // random exp.
oldprob = dist[i][scr]; // statisch
opf2 = (long double)oldprob*factor;
opf = (long double)1.0-pow(1.0-oldprob,factor);
experiment =((long double)found)/ ( ((long double)(max_experiment)));
//if(switcher==0 && oldprob<0.000001) { // old 0.00001 t3
//if(found==0)
//printf ("%Le %Le %Le %Le %Le %Le %i\n",experiment,opf,opf2,oldprob,factor,square,found);
if(scr>0 && (found==0 || (experiment>opf || experiment>opf2 || experiment<10.0/max_experiment))) {
if( (opf<=dist[i][scr-1]) && ( (opf > opf2) || (opf2 > dist[i][scr-1]))) {
experiment = opf;
}
if( ( (opf2 <= dist[i][scr-1]))) {
experiment = opf2;
}
}
if(experiment == 0.0) {
experiment = dist[i][scr-1];
}
dist[i][scr] = experiment;
//if(isnan(dist[i][scr]) || isinf(dist[i][scr])) dist[i][scr]=1.0;
if(para->DEBUG>1)printf("%li %li %Le\n", i, scr,dist[i][scr] );
}
// printf("%i %Le\n", i, expect);
}
return sdist;
}
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