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<HTML>
<HEAD>
<TITLE>
EMBOSS: oddcomp
</TITLE>
</HEAD>
<BODY BGCOLOR="#FFFFFF" text="#000000">
<table align=center border=0 cellspacing=0 cellpadding=0>
<tr><td valign=top>
<A HREF="/" ONMOUSEOVER="self.status='Go to the EMBOSS home page';return true"><img border=0 src="emboss_icon.jpg" alt="" width=150 height=48></a>
</td>
<td align=left valign=middle>
<b><font size="+6">
oddcomp
</font></b>
</td></tr>
</table>
<br>
<p>
<H2>
Function
</H2>
Find protein sequence regions with a biased composition
<H2>
Description
</H2>
<b>oddcomp</b> searches a series of protein files, reporting the identifier for
those that exceed a certain amino acid composition threshold in a
portion of the sequence.
<p>
<b>oddcomp</b> was written to answer the question 'which proteins contain at
least n X and m Y in p residues'.
<p>
One could search for serine rich or polyglutamine rich, collagen helix,
or similar proteins using this program.
<p>
<b>oddcomp</b> takes as input an amino acid composition data file in the same
format as the output from compseq. It can tolerate any word length
within reason for the memory capacity of the machine in question. Only
the first two fields in the composition data file are used: the word and
the number of occurrences. Any word not mentioned is initialised to a
threshold of zero.
<p>
<b>oddcomp</b> measures the amino acid composition (this can be dimers etc as
well as monomers) in a sliding window. If and when composition meets or
exceeds all the specified thresholds, the sequence is reported and
<b>oddcomp</b> moves to the next sequence. It does not report where in the
sequence it found the matching region, merely the sequence ID.
<p>
<b>oddcomp</b> was originally written to identify SR/RS containing proteins.
eg. specifying a window of forty amino acids containing at least 3 SR
and 4 RS words.
<p>
To search for a specific set of words in a sequence, edit the input
composition data file to delete any words from the input file in which
you are not interested. The search is a boolean AND so there must be
(from the short example above) at least 3 SR AND at least 4 RS for the
sequence to be reported. If your total words specified exceeds
window-wordlength+1 you will never get any hits.
<p>
Only one word size can be used and is specified at the top of the file
in compseq-style output.
<H2>
Usage
</H2>
<b>Here is a sample session with oddcomp</b>
<p>
To search for entries in swissprot with at least 1 SR AND at least 2 RS:
<p>
<p>
<table width="90%"><tr><td bgcolor="#CCFFFF"><pre>
% <b>oddcomp </b>
Find protein sequence regions with a biased composition
Input protein sequence(s): <b>tsw:*</b>
Program compseq output file: <b>oddcomp.comp</b>
Window size to consider (e.g. 30 aa) [30]: <b></b>
Output file [edd1_rat.oddcomp]: <b>out.odd</b>
</pre></td></tr></table><p>
<p>
<a href="#input.1">Go to the input files for this example</a><br><a href="#output.1">Go to the output files for this example</a><p><p>
<H2>
Command line arguments
</H2>
<table CELLSPACING=0 CELLPADDING=3 BGCOLOR="#f5f5ff" ><tr><td>
<pre>
Standard (Mandatory) qualifiers (* if not always prompted):
[-sequence] seqall Protein sequence(s) filename and optional
format, or reference (input USA)
[-infile] infile This is a file in the format of the output
produced by 'compseq' that is used to set
the minimum frequencies of words in this
analysis.
* -window integer [30] This is the size of window in which to
count.
Thus if you want to count frequencies in a
40 aa stretch you should enter 40 here.
(Integer 10 or more)
[-outfile] outfile [*.oddcomp] This is the results file.
Additional (Optional) qualifiers: (none)
Advanced (Unprompted) qualifiers:
-fullwindow toggle [N] Set this option on (Y) if you want the
window size to be set to the length of the
current protein. Otherwise, leave this
option unset, in which case you'll be
prompted for a window size to use.
-[no]ignorebz boolean [Y] The amino acid code B represents
Asparagine or Aspartic acid and the code Z
represents Glutamine or Glutamic acid.
These are not commonly used codes and you
may wish not to count words containing them,
just noting them in the count of 'Other'
words.
Associated qualifiers:
"-sequence" associated qualifiers
-sbegin1 integer Start of each sequence to be used
-send1 integer End of each sequence to be used
-sreverse1 boolean Reverse (if DNA)
-sask1 boolean Ask for begin/end/reverse
-snucleotide1 boolean Sequence is nucleotide
-sprotein1 boolean Sequence is protein
-slower1 boolean Make lower case
-supper1 boolean Make upper case
-sformat1 string Input sequence format
-sdbname1 string Database name
-sid1 string Entryname
-ufo1 string UFO features
-fformat1 string Features format
-fopenfile1 string Features file name
"-outfile" associated qualifiers
-odirectory3 string Output directory
General qualifiers:
-auto boolean Turn off prompts
-stdout boolean Write standard output
-filter boolean Read standard input, write standard output
-options boolean Prompt for standard and additional values
-debug boolean Write debug output to program.dbg
-verbose boolean Report some/full command line options
-help boolean Report command line options. More
information on associated and general
qualifiers can be found with -help -verbose
-warning boolean Report warnings
-error boolean Report errors
-fatal boolean Report fatal errors
-die boolean Report dying program messages
</pre>
</td></tr></table>
<P>
<table border cellspacing=0 cellpadding=3 bgcolor="#ccccff">
<tr bgcolor="#FFFFCC">
<th align="left" colspan=2>Standard (Mandatory) qualifiers</th>
<th align="left">Allowed values</th>
<th align="left">Default</th>
</tr>
<tr>
<td>[-sequence]<br>(Parameter 1)</td>
<td>Protein sequence(s) filename and optional format, or reference (input USA)</td>
<td>Readable sequence(s)</td>
<td><b>Required</b></td>
</tr>
<tr>
<td>[-infile]<br>(Parameter 2)</td>
<td>This is a file in the format of the output produced by 'compseq' that is used to set the minimum frequencies of words in this analysis.</td>
<td>Input file</td>
<td><b>Required</b></td>
</tr>
<tr>
<td>-window</td>
<td>This is the size of window in which to count.
Thus if you want to count frequencies in a 40 aa stretch you should enter 40 here.</td>
<td>Integer 10 or more</td>
<td>30</td>
</tr>
<tr>
<td>[-outfile]<br>(Parameter 3)</td>
<td>This is the results file.</td>
<td>Output file</td>
<td><i><*></i>.oddcomp</td>
</tr>
<tr bgcolor="#FFFFCC">
<th align="left" colspan=2>Additional (Optional) qualifiers</th>
<th align="left">Allowed values</th>
<th align="left">Default</th>
</tr>
<tr>
<td colspan=4>(none)</td>
</tr>
<tr bgcolor="#FFFFCC">
<th align="left" colspan=2>Advanced (Unprompted) qualifiers</th>
<th align="left">Allowed values</th>
<th align="left">Default</th>
</tr>
<tr>
<td>-fullwindow</td>
<td>Set this option on (Y) if you want the window size to be set to the length of the current protein. Otherwise, leave this option unset, in which case you'll be prompted for a window size to use.</td>
<td>Toggle value Yes/No</td>
<td>No</td>
</tr>
<tr>
<td>-[no]ignorebz</td>
<td>The amino acid code B represents Asparagine or Aspartic acid and the code Z represents Glutamine or Glutamic acid.
These are not commonly used codes and you may wish not to count words containing them, just noting them in the count of 'Other' words.</td>
<td>Boolean value Yes/No</td>
<td>Yes</td>
</tr>
</table>
<H2>
Input file format
</H2>
<a name="input.1"></a>
<h3>Input files for usage example </h3>
'tsw:*' is a sequence entry in the example protein database 'tsw'
<p>
<p><h3>File: oddcomp.comp</h3>
<table width="90%"><tr><td bgcolor="#FFCCFF">
<pre>
# Example input file for oddcomp
Word size 2
Total count 0
#
# Word Obs Count Obs Frequency Exp Frequency Obs/Exp Frequency
#
RS 2 0 0 0
SR 1 0 0 0
Other 0 0 0 0
</pre>
</td></tr></table><p>
<p>
The columns "Obs Frequency", "Exp Frequency" and "Obs/Exp Frequency" are
not required - they were simply included in this example to show the
similarity between this input file format and the output of the program
<A HREF="compseq.html">compseq</A>. A compseq output file can be used as the
input to <b>oddcomp</b> - the extra columns are ignored by
<b>oddcomp</b>.
<p>
A minimal composition input data file would look like this:
<hr>
<pre>
Word size 2
Total count 0
RS 2
SR 1
</pre>
<hr>
<p>
Blank lines and lines starting with '#' are ignored.
<p>
The first non-comment line should start with 'Word size' and will
specify the word size to use.
<p>
A line starting with the word 'Total' is required.
<p>
Anything after the line starting with the word 'Total' will be read as
word count data.
<p>
Word count data consists of a word to search for and the count of that
word to search for within the sliding window. The columns are separated
by one or more spaces or TAB characters. Anything after these two columns
will be ignored.
<H2>
Output file format
</H2>
<b>oddcomp</b> writes a list of sequence name identifiers.
<p>
<a name="output.1"></a>
<h3>Output files for usage example </h3>
<p><h3>File: out.odd</h3>
<table width="90%"><tr><td bgcolor="#CCFFCC">
<pre>
#
# Output from 'oddcomp'
#
# The Expected frequencies are taken from the file: ../../data/oddcomp.comp
#
# Word size: 2
CNR1B_FUGRU
CO9_FUGRU
FOS_FUGRU
RS7_FUGRU
# END #
</pre>
</td></tr></table><p>
<H2>
Data files
</H2>
None.
<H2>
Notes
</H2>
None.
<H2>
References
</H2>
None.
<H2>
Warnings
</H2>
None.
<H2>
Diagnostic Error Messages
</H2>
None.
<H2>
Exit status
</H2>
It always exits with a status of 0.
<H2>
Known bugs
</H2>
None.
<h2><a name="See also">See also</a></h2>
<table border cellpadding=4 bgcolor="#FFFFF0">
<tr><th>Program name</th><th>Description</th></tr>
<tr>
<td><a href="antigenic.html">antigenic</a></td>
<td>Finds antigenic sites in proteins</td>
</tr>
<tr>
<td><a href="digest.html">digest</a></td>
<td>Protein proteolytic enzyme or reagent cleavage digest</td>
</tr>
<tr>
<td><a href="epestfind.html">epestfind</a></td>
<td>Finds PEST motifs as potential proteolytic cleavage sites</td>
</tr>
<tr>
<td><a href="fuzzpro.html">fuzzpro</a></td>
<td>Protein pattern search</td>
</tr>
<tr>
<td><a href="fuzztran.html">fuzztran</a></td>
<td>Protein pattern search after translation</td>
</tr>
<tr>
<td><a href="helixturnhelix.html">helixturnhelix</a></td>
<td>Report nucleic acid binding motifs</td>
</tr>
<tr>
<td><a href="patmatdb.html">patmatdb</a></td>
<td>Search a protein sequence with a motif</td>
</tr>
<tr>
<td><a href="patmatmotifs.html">patmatmotifs</a></td>
<td>Search a PROSITE motif database with a protein sequence</td>
</tr>
<tr>
<td><a href="pepcoil.html">pepcoil</a></td>
<td>Predicts coiled coil regions</td>
</tr>
<tr>
<td><a href="preg.html">preg</a></td>
<td>Regular expression search of a protein sequence</td>
</tr>
<tr>
<td><a href="pscan.html">pscan</a></td>
<td>Scans proteins using PRINTS</td>
</tr>
<tr>
<td><a href="sigcleave.html">sigcleave</a></td>
<td>Reports protein signal cleavage sites</td>
</tr>
</table>
<H2>
Author(s)
</H2>
David Martin (dmartin © rfcgr.mrc.ac.uk)
<br>
<H2>
History
</H2>
Written (1999) - David Martin
<H2>
Target users
</H2>
This program is intended to be used by everyone and everything, from naive users to embedded scripts.
<H2>
Comments
</H2>
None
</BODY>
</HTML>
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