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<HTML>
<HEAD>
<TITLE>
EMBOSS: extractfeat
</TITLE>
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<table align=center border=0 cellspacing=0 cellpadding=0>
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<A HREF="/" ONMOUSEOVER="self.status='Go to the EMBOSS home page';return true"><img border=0 src="/images/emboss_icon.jpg" alt="" width=150 height=48></a>
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<b><font size="+6">
extractfeat
</font></b>
</td></tr>
</table>
<br>
<p>
<H2>
Wiki
</H2>
The master copies of EMBOSS documentation are available
at <a href="http://emboss.open-bio.org/wiki/Appdocs">
http://emboss.open-bio.org/wiki/Appdocs</a>
on the EMBOSS Wiki.
<p>
Please help by correcting and extending the Wiki pages.
<H2>
Function
</H2>
Extract features from sequence(s)
<H2>
Description
</H2>
<p><b>extractfeat</b> is a simple utility for extracting regions of a
sequence that are annotated as being a specified type of feature. It
reads one or more sequences, and writes out the sequences and features
of interest to an output sequence file. 'joined' features can either
be extracted as individual sequences, or as a single concatenated
sequence if the <tt>-join</tt> qualifier is used. If the feature is
annotated as being in the reverse sense of a nucleic acid sequence,
then that feature's sub-sequence is reverse-complemented before it is
written.</p>
<p>There are many options control exactly what parts of the feature
table are given in the output file. In addition, it is often useful
to have contextual information about a feature. There are options to
specify a number of positions before and/or after the specified
feature which will be reported in the output file.</p>
<H2>
Usage
</H2>
Here is a sample session with <b>extractfeat</b>
<p>
To write out the exons of a sequence:
<p>
<p>
<table width="90%"><tr><td bgcolor="#CCFFFF"><pre>
% <b>extractfeat tembl:x65921 -type exon </b>
Extract features from sequence(s)
output sequence [x65921.fasta]: <b></b>
</pre></td></tr></table><p>
<p>
<a href="#input.1">Go to the input files for this example</a><br><a href="#output.1">Go to the output files for this example</a><p><p>
<p>
<b>Example 2</b>
<p>
To write out the exons with 10 extra bases at the start and end so that you can inspect the splice sites:
<p>
<p>
<table width="90%"><tr><td bgcolor="#CCFFFF"><pre>
% <b>extractfeat tembl:x65921 -type exon -before 10 -after 10 </b>
Extract features from sequence(s)
output sequence [x65921.fasta]: <b></b>
</pre></td></tr></table><p>
<p>
<a href="#output.2">Go to the output files for this example</a><p><p>
<p>
<b>Example 3</b>
<p>
To write out the 10 bases around the start of all 'exon' features in the tembl database:
<p>
<p>
<table width="90%"><tr><td bgcolor="#CCFFFF"><pre>
% <b>extractfeat "tembl:*" -type exon -before 5 -after -5 </b>
Extract features from sequence(s)
output sequence [em498477.fasta]: <b></b>
</pre></td></tr></table><p>
<p>
<a href="#output.3">Go to the output files for this example</a><p><p>
<p>
<b>Example 4</b>
<p>
To extract the CDS region with the exons joined into one sequence:
<p>
<p>
<table width="90%"><tr><td bgcolor="#CCFFFF"><pre>
% <b>extractfeat tembl:x65921 -type CDS -join </b>
Extract features from sequence(s)
output sequence [x65921.fasta]: <b></b>
</pre></td></tr></table><p>
<p>
<a href="#output.4">Go to the output files for this example</a><p><p>
<p>
<b>Example 5</b>
<p>
To write out the 7 residues around all phosphorylated serine residues
<p>
<p>
<table width="90%"><tr><td bgcolor="#CCFFFF"><pre>
% <b>extractfeat "tsw:*" -type MOD_RES -value "phosphoserine*" -before 3 -after -4 </b>
Extract features from sequence(s)
output sequence [cru4_arath.fasta]: <b></b>
</pre></td></tr></table><p>
<p>
<a href="#output.5">Go to the output files for this example</a><p><p>
<H2>
Command line arguments
</H2>
<table CELLSPACING=0 CELLPADDING=3 BGCOLOR="#f5f5ff" ><tr><td>
<pre>
Extract features from sequence(s)
Version: EMBOSS:6.6.0.0
Standard (Mandatory) qualifiers:
[-sequence] seqall Sequence(s) filename and optional format, or
reference (input USA)
[-outseq] seqout [<sequence>.<format>] Sequence filename and
optional format (output USA)
Additional (Optional) qualifiers:
-before integer [0] If this value is greater than 0 then
that number of bases or residues before the
feature are included in the extracted
sequence. This allows you to get the context
of the feature. If this value is negative
then the start of the extracted sequence
will be this number of bases/residues before
the end of the feature. So a value of '10'
will start the extraction 10 bases/residues
before the start of the sequence, and a
value of '-10' will start the extraction 10
bases/residues before the end of the
feature. The output sequence will be padded
with 'N' or 'X' characters if the sequence
starts after the required start of the
extraction. (Any integer value)
-after integer [0] If this value is greater than 0 then
that number of bases or residues after the
feature are included in the extracted
sequence. This allows you to get the context
of the feature. If this value is negative
then the end of the extracted sequence will
be this number of bases/residues after the
start of the feature. So a value of '10'
will end the extraction 10 bases/residues
after the end of the sequence, and a value
of '-10' will end the extraction 10
bases/residues after the start of the
feature. The output sequence will be padded
with 'N' or 'X' characters if the sequence
ends before the required end of the
extraction. (Any integer value)
-source string [*] By default any feature source in the
feature table is shown. You can set this to
match any feature source you wish to show.
The source name is usually either the name
of the program that detected the feature or
it is the feature table (eg: EMBL) that the
feature came from.
The source may be wildcarded by using '*'.
If you wish to show more than one source,
separate their names with the character '|',
eg:
gene* | embl (Any string)
-type string [*] By default every feature in the feature
table is extracted. You can set this to be
any feature type you wish to extract.
See http://www.ebi.ac.uk/embl/WebFeat/ for a
list of the EMBL feature types and see the
Uniprot user manual in
http://www.uniprot.org/manual/sequence_annotation
for a list of the Uniprot feature types.
The type may be wildcarded by using '*'.
If you wish to extract more than one type,
separate their names with the character '|',
eg:
*UTR | intron (Any string)
-sense integer [0 - any sense, 1 - forward sense, -1 -
reverse sense] By default any feature type
in the feature table is extracted. You can
set this to match any feature sense you
wish. 0 - any sense, 1 - forward sense, -1 -
reverse sense (Any integer value)
-minscore float [0.0] Minimum score of feature to extract
(see also maxscore) (Any numeric value)
-maxscore float [0.0] Maximum score of feature to extract.
If both minscore and maxscore are zero (the
default), then any score is ignored (Any
numeric value)
-tag string [*] Tags are the types of extra values that
a feature may have. For example in the EMBL
feature table, a 'CDS' type of feature may
have the tags '/codon', '/codon_start',
'/db_xref', '/EC_number', '/evidence',
'/exception', '/function', '/gene',
'/label', '/map', '/note', '/number',
'/partial', '/product', '/protein_id',
'/pseudo', '/standard_name', '/translation',
'/transl_except', '/transl_table', or
'/usedin'. Some of these tags also have
values, for example '/gene' can have the
value of the gene name.
By default any feature tag in the feature
table is extracted. You can set this to
match any feature tag you wish to show.
The tag may be wildcarded by using '*'.
If you wish to extract more than one tag,
separate their names with the character '|',
eg:
gene | label (Any string)
-value string [*] Tag values are the values associated
with a feature tag. Tags are the types of
extra values that a feature may have. For
example in the EMBL feature table, a 'CDS'
type of feature may have the tags '/codon',
'/codon_start', '/db_xref', '/EC_number',
'/evidence', '/exception', '/function',
'/gene', '/label', '/map', '/note',
'/number', '/partial', '/product',
'/protein_id', '/pseudo', '/standard_name',
'/translation', '/transl_except',
'/transl_table', or '/usedin'. Only some of
these tags can have values, for example
'/gene' can have the value of the gene name.
By default any feature tag value in the
feature table is shown. You can set this to
match any feature tag value you wish to
show.
The tag value may be wildcarded by using
'*'.
If you wish to show more than one tag value,
separate their names with a space or the
character '|', eg:
pax* | 10 (Any string)
-join boolean [N] Some features, such as CDS (coding
sequence) and mRNA are composed of introns
concatenated together. There may be other
forms of 'joined' sequence, depending on the
feature table. If this option is set TRUE,
then any group of these features will be
output as a single sequence. If the 'before'
and 'after' qualifiers have been set, then
only the sequence before the first feature
and after the last feature are added.
-featinname boolean [N] To aid you in identifying the type of
feature that has been output, the type of
feature is added to the start of the
description of the output sequence.
Sometimes the description of a sequence is
lost in subsequent processing of the
sequences file, so it is useful for the type
to be a part of the sequence ID name. If
you set this to be TRUE then the name is
added to the ID name of the output sequence.
-describe string To aid you in identifying some further
properties of a feature that has been
output, this lets you specify one or more
tag names that should be added to the output
sequence Description text, together with
their values (if any). For example, if this
is set to be 'gene', then if any output
feature has the tag (for example)
'/gene=BRCA1' associated with it, then the
text '(gene=BRCA1)' will be added to the
Description line. Tags are the types of
extra values that a feature may have. For
example in the EMBL feature table, a 'CDS'
type of feature may have the tags '/codon',
'/codon_start', '/db_xref', '/EC_number',
'/evidence', '/exception', '/function',
'/gene', '/label', '/map', '/note',
'/number', '/partial', '/product',
'/protein_id', '/pseudo', '/standard_name',
'/translation', '/transl_except',
'/transl_table', or '/usedin'. Some of these
tags also have values, for example '/gene'
can have the value of the gene name.
By default no feature tag is displayed. You
can set this to match any feature tag you
wish to show.
The tag may be wildcarded by using '*'.
If you wish to extract more than one tag,
separate their names with the character '|',
eg:
gene | label (Any string)
Advanced (Unprompted) qualifiers: (none)
Associated qualifiers:
"-sequence" associated qualifiers
-sbegin1 integer Start of each sequence to be used
-send1 integer End of each sequence to be used
-sreverse1 boolean Reverse (if DNA)
-sask1 boolean Ask for begin/end/reverse
-snucleotide1 boolean Sequence is nucleotide
-sprotein1 boolean Sequence is protein
-slower1 boolean Make lower case
-supper1 boolean Make upper case
-scircular1 boolean Sequence is circular
-squick1 boolean Read id and sequence only
-sformat1 string Input sequence format
-iquery1 string Input query fields or ID list
-ioffset1 integer Input start position offset
-sdbname1 string Database name
-sid1 string Entryname
-ufo1 string UFO features
-fformat1 string Features format
-fopenfile1 string Features file name
"-outseq" associated qualifiers
-osformat2 string Output seq format
-osextension2 string File name extension
-osname2 string Base file name
-osdirectory2 string Output directory
-osdbname2 string Database name to add
-ossingle2 boolean Separate file for each entry
-oufo2 string UFO features
-offormat2 string Features format
-ofname2 string Features file name
-ofdirectory2 string Output directory
General qualifiers:
-auto boolean Turn off prompts
-stdout boolean Write first file to standard output
-filter boolean Read first file from standard input, write
first file to standard output
-options boolean Prompt for standard and additional values
-debug boolean Write debug output to program.dbg
-verbose boolean Report some/full command line options
-help boolean Report command line options and exit. More
information on associated and general
qualifiers can be found with -help -verbose
-warning boolean Report warnings
-error boolean Report errors
-fatal boolean Report fatal errors
-die boolean Report dying program messages
-version boolean Report version number and exit
</pre>
</td></tr></table>
<P>
<table border cellspacing=0 cellpadding=3 bgcolor="#ccccff">
<tr bgcolor="#FFFFCC">
<th align="left">Qualifier</th>
<th align="left">Type</th>
<th align="left">Description</th>
<th align="left">Allowed values</th>
<th align="left">Default</th>
</tr>
<tr bgcolor="#FFFFCC">
<th align="left" colspan=5>Standard (Mandatory) qualifiers</th>
</tr>
<tr bgcolor="#FFFFCC">
<td>[-sequence]<br>(Parameter 1)</td>
<td>seqall</td>
<td>Sequence(s) filename and optional format, or reference (input USA)</td>
<td>Readable sequence(s)</td>
<td><b>Required</b></td>
</tr>
<tr bgcolor="#FFFFCC">
<td>[-outseq]<br>(Parameter 2)</td>
<td>seqout</td>
<td>Sequence filename and optional format (output USA)</td>
<td>Writeable sequence</td>
<td><i><*></i>.<i>format</i></td>
</tr>
<tr bgcolor="#FFFFCC">
<th align="left" colspan=5>Additional (Optional) qualifiers</th>
</tr>
<tr bgcolor="#FFFFCC">
<td>-before</td>
<td>integer</td>
<td>If this value is greater than 0 then that number of bases or residues before the feature are included in the extracted sequence. This allows you to get the context of the feature. If this value is negative then the start of the extracted sequence will be this number of bases/residues before the end of the feature. So a value of '10' will start the extraction 10 bases/residues before the start of the sequence, and a value of '-10' will start the extraction 10 bases/residues before the end of the feature. The output sequence will be padded with 'N' or 'X' characters if the sequence starts after the required start of the extraction.</td>
<td>Any integer value</td>
<td>0</td>
</tr>
<tr bgcolor="#FFFFCC">
<td>-after</td>
<td>integer</td>
<td>If this value is greater than 0 then that number of bases or residues after the feature are included in the extracted sequence. This allows you to get the context of the feature. If this value is negative then the end of the extracted sequence will be this number of bases/residues after the start of the feature. So a value of '10' will end the extraction 10 bases/residues after the end of the sequence, and a value of '-10' will end the extraction 10 bases/residues after the start of the feature. The output sequence will be padded with 'N' or 'X' characters if the sequence ends before the required end of the extraction.</td>
<td>Any integer value</td>
<td>0</td>
</tr>
<tr bgcolor="#FFFFCC">
<td>-source</td>
<td>string</td>
<td>By default any feature source in the feature table is shown. You can set this to match any feature source you wish to show.
The source name is usually either the name of the program that detected the feature or it is the feature table (eg: EMBL) that the feature came from.
The source may be wildcarded by using '*'.
If you wish to show more than one source, separate their names with the character '|', eg:
gene* | embl</td>
<td>Any string</td>
<td>*</td>
</tr>
<tr bgcolor="#FFFFCC">
<td>-type</td>
<td>string</td>
<td>By default every feature in the feature table is extracted. You can set this to be any feature type you wish to extract.
See http://www.ebi.ac.uk/embl/WebFeat/ for a list of the EMBL feature types and see the Uniprot user manual in http://www.uniprot.org/manual/sequence_annotation for a list of the Uniprot feature types.
The type may be wildcarded by using '*'.
If you wish to extract more than one type, separate their names with the character '|', eg:
*UTR | intron</td>
<td>Any string</td>
<td>*</td>
</tr>
<tr bgcolor="#FFFFCC">
<td>-sense</td>
<td>integer</td>
<td>By default any feature type in the feature table is extracted. You can set this to match any feature sense you wish. 0 - any sense, 1 - forward sense, -1 - reverse sense</td>
<td>Any integer value</td>
<td>0 - any sense, 1 - forward sense, -1 - reverse sense</td>
</tr>
<tr bgcolor="#FFFFCC">
<td>-minscore</td>
<td>float</td>
<td>Minimum score of feature to extract (see also maxscore)</td>
<td>Any numeric value</td>
<td>0.0</td>
</tr>
<tr bgcolor="#FFFFCC">
<td>-maxscore</td>
<td>float</td>
<td>Maximum score of feature to extract.
If both minscore and maxscore are zero (the default), then any score is ignored</td>
<td>Any numeric value</td>
<td>0.0</td>
</tr>
<tr bgcolor="#FFFFCC">
<td>-tag</td>
<td>string</td>
<td>Tags are the types of extra values that a feature may have. For example in the EMBL feature table, a 'CDS' type of feature may have the tags '/codon', '/codon_start', '/db_xref', '/EC_number', '/evidence', '/exception', '/function', '/gene', '/label', '/map', '/note', '/number', '/partial', '/product', '/protein_id', '/pseudo', '/standard_name', '/translation', '/transl_except', '/transl_table', or '/usedin'. Some of these tags also have values, for example '/gene' can have the value of the gene name.
By default any feature tag in the feature table is extracted. You can set this to match any feature tag you wish to show.
The tag may be wildcarded by using '*'.
If you wish to extract more than one tag, separate their names with the character '|', eg:
gene | label</td>
<td>Any string</td>
<td>*</td>
</tr>
<tr bgcolor="#FFFFCC">
<td>-value</td>
<td>string</td>
<td>Tag values are the values associated with a feature tag. Tags are the types of extra values that a feature may have. For example in the EMBL feature table, a 'CDS' type of feature may have the tags '/codon', '/codon_start', '/db_xref', '/EC_number', '/evidence', '/exception', '/function', '/gene', '/label', '/map', '/note', '/number', '/partial', '/product', '/protein_id', '/pseudo', '/standard_name', '/translation', '/transl_except', '/transl_table', or '/usedin'. Only some of these tags can have values, for example '/gene' can have the value of the gene name. By default any feature tag value in the feature table is shown. You can set this to match any feature tag value you wish to show.
The tag value may be wildcarded by using '*'.
If you wish to show more than one tag value, separate their names with a space or the character '|', eg:
pax* | 10</td>
<td>Any string</td>
<td>*</td>
</tr>
<tr bgcolor="#FFFFCC">
<td>-join</td>
<td>boolean</td>
<td>Some features, such as CDS (coding sequence) and mRNA are composed of introns concatenated together. There may be other forms of 'joined' sequence, depending on the feature table. If this option is set TRUE, then any group of these features will be output as a single sequence. If the 'before' and 'after' qualifiers have been set, then only the sequence before the first feature and after the last feature are added.</td>
<td>Boolean value Yes/No</td>
<td>No</td>
</tr>
<tr bgcolor="#FFFFCC">
<td>-featinname</td>
<td>boolean</td>
<td>To aid you in identifying the type of feature that has been output, the type of feature is added to the start of the description of the output sequence. Sometimes the description of a sequence is lost in subsequent processing of the sequences file, so it is useful for the type to be a part of the sequence ID name. If you set this to be TRUE then the name is added to the ID name of the output sequence.</td>
<td>Boolean value Yes/No</td>
<td>No</td>
</tr>
<tr bgcolor="#FFFFCC">
<td>-describe</td>
<td>string</td>
<td>To aid you in identifying some further properties of a feature that has been output, this lets you specify one or more tag names that should be added to the output sequence Description text, together with their values (if any). For example, if this is set to be 'gene', then if any output feature has the tag (for example) '/gene=BRCA1' associated with it, then the text '(gene=BRCA1)' will be added to the Description line. Tags are the types of extra values that a feature may have. For example in the EMBL feature table, a 'CDS' type of feature may have the tags '/codon', '/codon_start', '/db_xref', '/EC_number', '/evidence', '/exception', '/function', '/gene', '/label', '/map', '/note', '/number', '/partial', '/product', '/protein_id', '/pseudo', '/standard_name', '/translation', '/transl_except', '/transl_table', or '/usedin'. Some of these tags also have values, for example '/gene' can have the value of the gene name.
By default no feature tag is displayed. You can set this to match any feature tag you wish to show.
The tag may be wildcarded by using '*'.
If you wish to extract more than one tag, separate their names with the character '|', eg:
gene | label</td>
<td>Any string</td>
<td> </td>
</tr>
<tr bgcolor="#FFFFCC">
<th align="left" colspan=5>Advanced (Unprompted) qualifiers</th>
</tr>
<tr>
<td colspan=5>(none)</td>
</tr>
<tr bgcolor="#FFFFCC">
<th align="left" colspan=5>Associated qualifiers</th>
</tr>
<tr bgcolor="#FFFFCC">
<td align="left" colspan=5>"-sequence" associated seqall qualifiers
</td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -sbegin1<br>-sbegin_sequence</td>
<td>integer</td>
<td>Start of each sequence to be used</td>
<td>Any integer value</td>
<td>0</td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -send1<br>-send_sequence</td>
<td>integer</td>
<td>End of each sequence to be used</td>
<td>Any integer value</td>
<td>0</td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -sreverse1<br>-sreverse_sequence</td>
<td>boolean</td>
<td>Reverse (if DNA)</td>
<td>Boolean value Yes/No</td>
<td>N</td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -sask1<br>-sask_sequence</td>
<td>boolean</td>
<td>Ask for begin/end/reverse</td>
<td>Boolean value Yes/No</td>
<td>N</td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -snucleotide1<br>-snucleotide_sequence</td>
<td>boolean</td>
<td>Sequence is nucleotide</td>
<td>Boolean value Yes/No</td>
<td>N</td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -sprotein1<br>-sprotein_sequence</td>
<td>boolean</td>
<td>Sequence is protein</td>
<td>Boolean value Yes/No</td>
<td>N</td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -slower1<br>-slower_sequence</td>
<td>boolean</td>
<td>Make lower case</td>
<td>Boolean value Yes/No</td>
<td>N</td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -supper1<br>-supper_sequence</td>
<td>boolean</td>
<td>Make upper case</td>
<td>Boolean value Yes/No</td>
<td>N</td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -scircular1<br>-scircular_sequence</td>
<td>boolean</td>
<td>Sequence is circular</td>
<td>Boolean value Yes/No</td>
<td>N</td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -squick1<br>-squick_sequence</td>
<td>boolean</td>
<td>Read id and sequence only</td>
<td>Boolean value Yes/No</td>
<td>N</td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -sformat1<br>-sformat_sequence</td>
<td>string</td>
<td>Input sequence format</td>
<td>Any string</td>
<td> </td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -iquery1<br>-iquery_sequence</td>
<td>string</td>
<td>Input query fields or ID list</td>
<td>Any string</td>
<td> </td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -ioffset1<br>-ioffset_sequence</td>
<td>integer</td>
<td>Input start position offset</td>
<td>Any integer value</td>
<td>0</td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -sdbname1<br>-sdbname_sequence</td>
<td>string</td>
<td>Database name</td>
<td>Any string</td>
<td> </td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -sid1<br>-sid_sequence</td>
<td>string</td>
<td>Entryname</td>
<td>Any string</td>
<td> </td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -ufo1<br>-ufo_sequence</td>
<td>string</td>
<td>UFO features</td>
<td>Any string</td>
<td> </td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -fformat1<br>-fformat_sequence</td>
<td>string</td>
<td>Features format</td>
<td>Any string</td>
<td> </td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -fopenfile1<br>-fopenfile_sequence</td>
<td>string</td>
<td>Features file name</td>
<td>Any string</td>
<td> </td>
</tr>
<tr bgcolor="#FFFFCC">
<td align="left" colspan=5>"-outseq" associated seqout qualifiers
</td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -osformat2<br>-osformat_outseq</td>
<td>string</td>
<td>Output seq format</td>
<td>Any string</td>
<td> </td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -osextension2<br>-osextension_outseq</td>
<td>string</td>
<td>File name extension</td>
<td>Any string</td>
<td> </td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -osname2<br>-osname_outseq</td>
<td>string</td>
<td>Base file name</td>
<td>Any string</td>
<td> </td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -osdirectory2<br>-osdirectory_outseq</td>
<td>string</td>
<td>Output directory</td>
<td>Any string</td>
<td> </td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -osdbname2<br>-osdbname_outseq</td>
<td>string</td>
<td>Database name to add</td>
<td>Any string</td>
<td> </td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -ossingle2<br>-ossingle_outseq</td>
<td>boolean</td>
<td>Separate file for each entry</td>
<td>Boolean value Yes/No</td>
<td>N</td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -oufo2<br>-oufo_outseq</td>
<td>string</td>
<td>UFO features</td>
<td>Any string</td>
<td> </td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -offormat2<br>-offormat_outseq</td>
<td>string</td>
<td>Features format</td>
<td>Any string</td>
<td> </td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -ofname2<br>-ofname_outseq</td>
<td>string</td>
<td>Features file name</td>
<td>Any string</td>
<td> </td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -ofdirectory2<br>-ofdirectory_outseq</td>
<td>string</td>
<td>Output directory</td>
<td>Any string</td>
<td> </td>
</tr>
<tr bgcolor="#FFFFCC">
<th align="left" colspan=5>General qualifiers</th>
</tr>
<tr bgcolor="#FFFFCC">
<td> -auto</td>
<td>boolean</td>
<td>Turn off prompts</td>
<td>Boolean value Yes/No</td>
<td>N</td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -stdout</td>
<td>boolean</td>
<td>Write first file to standard output</td>
<td>Boolean value Yes/No</td>
<td>N</td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -filter</td>
<td>boolean</td>
<td>Read first file from standard input, write first file to standard output</td>
<td>Boolean value Yes/No</td>
<td>N</td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -options</td>
<td>boolean</td>
<td>Prompt for standard and additional values</td>
<td>Boolean value Yes/No</td>
<td>N</td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -debug</td>
<td>boolean</td>
<td>Write debug output to program.dbg</td>
<td>Boolean value Yes/No</td>
<td>N</td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -verbose</td>
<td>boolean</td>
<td>Report some/full command line options</td>
<td>Boolean value Yes/No</td>
<td>Y</td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -help</td>
<td>boolean</td>
<td>Report command line options and exit. More information on associated and general qualifiers can be found with -help -verbose</td>
<td>Boolean value Yes/No</td>
<td>N</td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -warning</td>
<td>boolean</td>
<td>Report warnings</td>
<td>Boolean value Yes/No</td>
<td>Y</td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -error</td>
<td>boolean</td>
<td>Report errors</td>
<td>Boolean value Yes/No</td>
<td>Y</td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -fatal</td>
<td>boolean</td>
<td>Report fatal errors</td>
<td>Boolean value Yes/No</td>
<td>Y</td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -die</td>
<td>boolean</td>
<td>Report dying program messages</td>
<td>Boolean value Yes/No</td>
<td>Y</td>
</tr>
<tr bgcolor="#FFFFCC">
<td> -version</td>
<td>boolean</td>
<td>Report version number and exit</td>
<td>Boolean value Yes/No</td>
<td>N</td>
</tr>
</table>
<H2>
Input file format
</H2>
<b>extractfeat</b> reads normal sequences with features.
<p>
Feature tables in Swissprot, EMBL, GFF, etc. format can be added using
'-ufo featurefile' on the command line.
<p>
<a name="input.1"></a>
<h3>Input files for usage example </h3>
'tembl:x65921' is a sequence entry in the example nucleic acid database 'tembl'
<p>
<p><h3>Database entry: tembl:x65921</h3>
<table width="90%"><tr><td bgcolor="#FFCCFF">
<pre>
ID X65921; SV 1; linear; genomic DNA; STD; HUM; 2016 BP.
XX
AC X65921; S45242;
XX
DT 13-MAY-1992 (Rel. 31, Created)
DT 14-NOV-2006 (Rel. 89, Last updated, Version 7)
XX
DE H.sapiens fau 1 gene
XX
KW fau 1 gene.
XX
OS Homo sapiens (human)
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
XX
RN [1]
RP 1-2016
RA Kas K.;
RT ;
RL Submitted (29-APR-1992) to the INSDC.
RL K. Kas, University of Antwerp, Dept of Biochemistry T3.22,
RL Universiteitsplein 1, 2610 Wilrijk, BELGIUM
XX
RN [2]
RP 1-2016
RX DOI; 10.1016/0006-291X(92)91286-Y.
RX PUBMED; 1326960.
RA Kas K., Michiels L., Merregaert J.;
RT "Genomic structure and expression of the human fau gene: encoding the
RT ribosomal protein S30 fused to a ubiquitin-like protein";
RL Biochem. Biophys. Res. Commun. 187(2):927-933(1992).
XX
DR Ensembl-Gn; ENSG00000149806; Homo_sapiens.
DR Ensembl-Tr; ENST00000279259; Homo_sapiens.
DR Ensembl-Tr; ENST00000434372; Homo_sapiens.
DR Ensembl-Tr; ENST00000525297; Homo_sapiens.
DR Ensembl-Tr; ENST00000526555; Homo_sapiens.
DR Ensembl-Tr; ENST00000527548; Homo_sapiens.
DR Ensembl-Tr; ENST00000529259; Homo_sapiens.
DR Ensembl-Tr; ENST00000529639; Homo_sapiens.
DR Ensembl-Tr; ENST00000531743; Homo_sapiens.
DR GDB; 191789.
DR GDB; 191790.
DR GDB; 354872.
DR GDB; 4590236.
XX
FH Key Location/Qualifiers
FH
FT source 1..2016
<font color=red> [Part of this file has been deleted for brevity]</font>
FT RAKRRMQYNRRFVNVVPTFGKKKGPNANS"
FT intron 857..950
FT /number=2
FT exon 951..1095
FT /number=3
FT intron 1096..1556
FT /number=3
FT exon 1557..1612
FT /number=4
FT intron 1613..1786
FT /number=4
FT exon 1787..>1912
FT /number=5
FT polyA_signal 1938..1943
XX
SQ Sequence 2016 BP; 421 A; 562 C; 538 G; 495 T; 0 other;
ctaccatttt ccctctcgat tctatatgta cactcgggac aagttctcct gatcgaaaac 60
ggcaaaacta aggccccaag taggaatgcc ttagttttcg gggttaacaa tgattaacac 120
tgagcctcac acccacgcga tgccctcagc tcctcgctca gcgctctcac caacagccgt 180
agcccgcagc cccgctggac accggttctc catccccgca gcgtagcccg gaacatggta 240
gctgccatct ttacctgcta cgccagcctt ctgtgcgcgc aactgtctgg tcccgccccg 300
tcctgcgcga gctgctgccc aggcaggttc gccggtgcga gcgtaaaggg gcggagctag 360
gactgccttg ggcggtacaa atagcaggga accgcgcggt cgctcagcag tgacgtgaca 420
cgcagcccac ggtctgtact gacgcgccct cgcttcttcc tctttctcga ctccatcttc 480
gcggtagctg ggaccgccgt tcaggtaaga atggggcctt ggctggatcc gaagggcttg 540
tagcaggttg gctgcggggt cagaaggcgc ggggggaacc gaagaacggg gcctgctccg 600
tggccctgct ccagtcccta tccgaactcc ttgggaggca ctggccttcc gcacgtgagc 660
cgccgcgacc accatcccgt cgcgatcgtt tctggaccgc tttccactcc caaatctcct 720
ttatcccaga gcatttcttg gcttctctta caagccgtct tttctttact cagtcgccaa 780
tatgcagctc tttgtccgcg cccaggagct acacaccttc gaggtgaccg gccaggaaac 840
ggtcgcccag atcaaggtaa ggctgcttgg tgcgccctgg gttccatttt cttgtgctct 900
tcactctcgc ggcccgaggg aacgcttacg agccttatct ttccctgtag gctcatgtag 960
cctcactgga gggcattgcc ccggaagatc aagtcgtgct cctggcaggc gcgcccctgg 1020
aggatgaggc cactctgggc cagtgcgggg tggaggccct gactaccctg gaagtagcag 1080
gccgcatgct tggaggtgag tgagagagga atgttctttg aagtaccggt aagcgtctag 1140
tgagtgtggg gtgcatagtc ctgacagctg agtgtcacac ctatggtaat agagtacttc 1200
tcactgtctt cagttcagag tgattcttcc tgtttacatc cctcatgttg aacacagacg 1260
tccatgggag actgagccag agtgtagttg tatttcagtc acatcacgag atcctagtct 1320
ggttatcagc ttccacacta aaaattaggt cagaccaggc cccaaagtgc tctataaatt 1380
agaagctgga agatcctgaa atgaaactta agatttcaag gtcaaatatc tgcaactttg 1440
ttctcattac ctattgggcg cagcttctct ttaaaggctt gaattgagaa aagaggggtt 1500
ctgctgggtg gcaccttctt gctcttacct gctggtgcct tcctttccca ctacaggtaa 1560
agtccatggt tccctggccc gtgctggaaa agtgagaggt cagactccta aggtgagtga 1620
gagtattagt ggtcatggtg ttaggacttt ttttcctttc acagctaaac caagtccctg 1680
ggctcttact cggtttgcct tctccctccc tggagatgag cctgagggaa gggatgctag 1740
gtgtggaaga caggaaccag ggcctgatta accttccctt ctccaggtgg ccaaacagga 1800
gaagaagaag aagaagacag gtcgggctaa gcggcggatg cagtacaacc ggcgctttgt 1860
caacgttgtg cccacctttg gcaagaagaa gggccccaat gccaactctt aagtcttttg 1920
taattctggc tttctctaat aaaaaagcca cttagttcag tcatcgcatt gtttcatctt 1980
tacttgcaag gcctcaggga gaggtgtgct tctcgg 2016
//
</pre>
</td></tr></table><p>
<H2>
Output file format
</H2>
<a name="output.1"></a>
<h3>Output files for usage example </h3>
<p><h3>File: x65921.fasta</h3>
<table width="90%"><tr><td bgcolor="#CCFFCC">
<pre>
>X65921_408_504 [exon] H.sapiens fau 1 gene
cagtgacgtgacacgcagcccacggtctgtactgacgcgccctcgcttcttcctctttct
cgactccatcttcgcggtagctgggaccgccgttcag
>X65921_774_856 [exon] H.sapiens fau 1 gene
tcgccaatatgcagctctttgtccgcgcccaggagctacacaccttcgaggtgaccggcc
aggaaacggtcgcccagatcaag
>X65921_951_1095 [exon] H.sapiens fau 1 gene
gctcatgtagcctcactggagggcattgccccggaagatcaagtcgtgctcctggcaggc
gcgcccctggaggatgaggccactctgggccagtgcggggtggaggccctgactaccctg
gaagtagcaggccgcatgcttggag
>X65921_1557_1612 [exon] H.sapiens fau 1 gene
gtaaagtccatggttccctggcccgtgctggaaaagtgagaggtcagactcctaag
>X65921_1787_1912 [exon] H.sapiens fau 1 gene
gtggccaaacaggagaagaagaagaagaagacaggtcgggctaagcggcggatgcagtac
aaccggcgctttgtcaacgttgtgcccacctttggcaagaagaagggccccaatgccaac
tcttaa
</pre>
</td></tr></table><p>
<a name="output.2"></a>
<h3>Output files for usage example 2</h3>
<p><h3>File: x65921.fasta</h3>
<table width="90%"><tr><td bgcolor="#CCFFCC">
<pre>
>X65921_408_504 [exon] H.sapiens fau 1 gene
ggtcgctcagcagtgacgtgacacgcagcccacggtctgtactgacgcgccctcgcttct
tcctctttctcgactccatcttcgcggtagctgggaccgccgttcaggtaagaatgg
>X65921_774_856 [exon] H.sapiens fau 1 gene
ctttactcagtcgccaatatgcagctctttgtccgcgcccaggagctacacaccttcgag
gtgaccggccaggaaacggtcgcccagatcaaggtaaggctgc
>X65921_951_1095 [exon] H.sapiens fau 1 gene
ttccctgtaggctcatgtagcctcactggagggcattgccccggaagatcaagtcgtgct
cctggcaggcgcgcccctggaggatgaggccactctgggccagtgcggggtggaggccct
gactaccctggaagtagcaggccgcatgcttggaggtgagtgaga
>X65921_1557_1612 [exon] H.sapiens fau 1 gene
cccactacaggtaaagtccatggttccctggcccgtgctggaaaagtgagaggtcagact
cctaaggtgagtgaga
>X65921_1787_1912 [exon] H.sapiens fau 1 gene
ccttctccaggtggccaaacaggagaagaagaagaagaagacaggtcgggctaagcggcg
gatgcagtacaaccggcgctttgtcaacgttgtgcccacctttggcaagaagaagggccc
caatgccaactcttaagtcttttgta
</pre>
</td></tr></table><p>
<a name="output.3"></a>
<h3>Output files for usage example 3</h3>
<p><h3>File: em498477.fasta</h3>
<table width="90%"><tr><td bgcolor="#CCFFCC">
<pre>
>X65921_408_504 [exon] H.sapiens fau 1 gene
ctcagcagtg
>X65921_774_856 [exon] H.sapiens fau 1 gene
ctcagtcgcc
>X65921_951_1095 [exon] H.sapiens fau 1 gene
tgtaggctca
>X65921_1557_1612 [exon] H.sapiens fau 1 gene
tacaggtaaa
>X65921_1787_1912 [exon] H.sapiens fau 1 gene
tccaggtggc
>K00650_889_1029 [exon] Human fos proto-oncogene (c-fos), complete cds.
ccacgatgat
>K00650_1783_2034 [exon] Human fos proto-oncogene (c-fos), complete cds.
tctaggactt
>K00650_2466_2573 [exon] Human fos proto-oncogene (c-fos), complete cds.
tctagttatc
>K00650_2688_3329 [exon] Human fos proto-oncogene (c-fos), complete cds.
tacaggagac
>D00596_1001_1205 [exon] Homo sapiens gene for thymidylate synthase, complete cds.
gcgccatgcc
>D00596_2895_2968 [exon] Homo sapiens gene for thymidylate synthase, complete cds.
ttcagatgaa
>D00596_5396_5570 [exon] Homo sapiens gene for thymidylate synthase, complete cds.
tccagggatc
>D00596_11843_11944 [exon] Homo sapiens gene for thymidylate synthase, complete cds.
tacagattat
>D00596_13449_13624 [exon] Homo sapiens gene for thymidylate synthase, complete cds.
ctcagatctt
>D00596_14133_14204 [exon] Homo sapiens gene for thymidylate synthase, complete cds.
tatagccagg
>D00596_15613_15750 [exon] Homo sapiens gene for thymidylate synthase, complete cds.
tttagcttca
>AB009071_67_155 [exon] Homo sapiens HERG gene, complete cds.
cccgcccatg
>AB009071_356_586 [exon] Homo sapiens HERG gene, complete cds.
cctaggccgt
>AB009071_768_932 [exon] Homo sapiens HERG gene, complete cds.
tgcagggagc
>AB009071_1137_1580 [exon] Homo sapiens HERG gene, complete cds.
cgcaggccgc
>AB009071_1742_1953 [exon] Homo sapiens HERG gene, complete cds.
cctaggggcc
>AB009071_2168_2596 [exon] Homo sapiens HERG gene, complete cds.
tgcaggtcct
>AB009071_2765_3152 [exon] Homo sapiens HERG gene, complete cds.
cccagctgat
>AB009071_3332_3531 [exon] Homo sapiens HERG gene, complete cds.
cccagccctc
>AB009071_3716_3968 [exon] Homo sapiens HERG gene, complete cds.
cccaggtgct
<font color=red> [Part of this file has been deleted for brevity]</font>
aacagctcct
>U01317_39414_39558 [exon] Human beta globin region on chromosome 11.
tccacacact
>U01317_39681_39903 [exon] Human beta globin region on chromosome 11.
cacaggctcc
>U01317_40770_40985 [exon] Human beta globin region on chromosome 11.
aacagctcct
>U01317_45710_45800 [exon] Human beta globin region on chromosome 11.
acactgtagt
>U01317_45922_46145 [exon] Human beta globin region on chromosome 11.
cacagtctcc
>U01317_46997_47124 [exon] Human beta globin region on chromosome 11.
cccagctctt
>U01317_54740_54881 [exon] Human beta globin region on chromosome 11.
tgcttacact
>U01317_55010_55232 [exon] Human beta globin region on chromosome 11.
ctcagattac
>U01317_56131_56389 [exon] Human beta globin region on chromosome 11.
cgcagctctt
>U01317_62137_62278 [exon] Human beta globin region on chromosome 11.
tgcttacatt
>U01317_62187_62278 [exon] Human beta globin region on chromosome 11.
acaccatggt
>U01317_62390_62408 [exon] Human beta globin region on chromosome 11.
attggtctat
>U01317_62409_62631 [exon] Human beta globin region on chromosome 11.
cttaggctgc
>U01317_63482_63742 [exon] Human beta globin region on chromosome 11.
cacagctcct
>M23100_1541_1923 [exon] Human insulin receptor (INSR) gene, exon 1, clones lambda-hINSR-(1-13).
ctccgggccc
>M23100_1542_1923 [exon] Human insulin receptor (INSR) gene, exon 1, clones lambda-hINSR-(1-13).
tccgggcccc
>M23100_1548_1923 [exon] Human insulin receptor (INSR) gene, exon 1, clones lambda-hINSR-(1-13).
ccccgagatc
>V00451_363_460 [exon] Glycine max leghemoglobin gene or pseudogene (no mRNA detected).
gaaatatggg
>V00451_555_663 [exon] Glycine max leghemoglobin gene or pseudogene (no mRNA detected).
aataggatat
>V00451_2182_2286 [exon] Glycine max leghemoglobin gene or pseudogene (no mRNA detected).
tgtaggtgcg
>V00451_3065_3208 [exon] Glycine max leghemoglobin gene or pseudogene (no mRNA detected).
cgtaggtggt
>M11903_351_499 [exon] Rattus norvegicus androgen-responsive protein precursor (Svf) gene, exons 1 and 1A, alternatively spliced.
cctgtaggca
>M11903_401_499 [exon] Rattus norvegicus androgen-responsive protein precursor (Svf) gene, exons 1 and 1A, alternatively spliced.
gctctcagtc
>M11904_109_445 [exon] Rattus norvegicus androgen-responsive protein precursor (Svf) gene, exon 2 and complete cds.
aacagaaaaa
>M11905_233_420 [exon] Rattus norvegicus androgen-responsive protein precursor (Svf) gene, exon 3.
cacaggttgt
</pre>
</td></tr></table><p>
<a name="output.4"></a>
<h3>Output files for usage example 4</h3>
<p><h3>File: x65921.fasta</h3>
<table width="90%"><tr><td bgcolor="#CCFFCC">
<pre>
>X65921_782_1912 [CDS] H.sapiens fau 1 gene
atgcagctctttgtccgcgcccaggagctacacaccttcgaggtgaccggccaggaaacg
gtcgcccagatcaaggctcatgtagcctcactggagggcattgccccggaagatcaagtc
gtgctcctggcaggcgcgcccctggaggatgaggccactctgggccagtgcggggtggag
gccctgactaccctggaagtagcaggccgcatgcttggaggtaaagtccatggttccctg
gcccgtgctggaaaagtgagaggtcagactcctaaggtggccaaacaggagaagaagaag
aagaagacaggtcgggctaagcggcggatgcagtacaaccggcgctttgtcaacgttgtg
cccacctttggcaagaagaagggccccaatgccaactcttaa
</pre>
</td></tr></table><p>
<a name="output.5"></a>
<h3>Output files for usage example 5</h3>
<p><h3>File: cru4_arath.fasta</h3>
<table width="90%"><tr><td bgcolor="#CCFFCC">
<pre>
>CRU4_ARATH_52_52 [post_translationally_modified_region] 12S seed storage protein CRU4 (Cruciferin 4) (AtCRU4) (Cruciferin A1) (Legumin-type globulin storage protein CRU4) (12S seed storage protein CRU4 alpha chain) (12S seed storage protein CRU4 acidic chain) (12S seed storage protein CRU4 beta chain) (12S seed storage protein CRU4 basic chain) (Precursor)
VLKSEAG
>CRU4_ARATH_96_96 [post_translationally_modified_region] 12S seed storage protein CRU4 (Cruciferin 4) (AtCRU4) (Cruciferin A1) (Legumin-type globulin storage protein CRU4) (12S seed storage protein CRU4 alpha chain) (12S seed storage protein CRU4 acidic chain) (12S seed storage protein CRU4 beta chain) (12S seed storage protein CRU4 basic chain) (Precursor)
AKLSFVA
>CRU4_ARATH_314_314 [post_translationally_modified_region] 12S seed storage protein CRU4 (Cruciferin 4) (AtCRU4) (Cruciferin A1) (Legumin-type globulin storage protein CRU4) (12S seed storage protein CRU4 alpha chain) (12S seed storage protein CRU4 acidic chain) (12S seed storage protein CRU4 beta chain) (12S seed storage protein CRU4 basic chain) (Precursor)
GYISTLN
>AQP1_HUMAN_247_247 [post_translationally_modified_region] Aquaporin-1 (AQP-1) (Aquaporin-CHIP) (Urine water channel) (Water channel protein for red blood cells and kidney proximal tubule)
VWTSGQV
>AQP1_HUMAN_262_262 [post_translationally_modified_region] Aquaporin-1 (AQP-1) (Aquaporin-CHIP) (Urine water channel) (Water channel protein for red blood cells and kidney proximal tubule)
DINSRVE
>GCN4_YEAST_17_17 [post_translationally_modified_region] General control protein GCN4 (Amino acid biosynthesis regulatory protein)
MGFSPLD
>GCN4_YEAST_218_218 [post_translationally_modified_region] General control protein GCN4 (Amino acid biosynthesis regulatory protein)
IPLSPIV
>OPSD_HUMAN_334_334 [post_translationally_modified_region] Rhodopsin (Opsin-2)
DEASATV
>OPSD_HUMAN_338_338 [post_translationally_modified_region] Rhodopsin (Opsin-2)
ATVSKTE
>OPSD_HUMAN_343_343 [post_translationally_modified_region] Rhodopsin (Opsin-2)
TETSQVA
>PAX3_HUMAN_201_201 [post_translationally_modified_region] Paired box protein Pax-3 (HuP2)
APQSDEG
>PAX3_HUMAN_205_205 [post_translationally_modified_region] Paired box protein Pax-3 (HuP2)
DEGSDID
>PAX3_HUMAN_209_209 [post_translationally_modified_region] Paired box protein Pax-3 (HuP2)
DIDSEPD
>PAXI_HUMAN_83_83 [post_translationally_modified_region] Paxillin
QPQSSSP
>PAXI_HUMAN_84_84 [post_translationally_modified_region] Paxillin
PQSSSPV
>PAXI_HUMAN_85_85 [post_translationally_modified_region] Paxillin
QSSSPVY
>PAXI_HUMAN_106_106 [post_translationally_modified_region] Paxillin
SVGSPCS
>PAXI_HUMAN_109_109 [post_translationally_modified_region] Paxillin
SPCSRVG
>PAXI_HUMAN_126_126 [post_translationally_modified_region] Paxillin
KQKSAEP
>PAXI_HUMAN_130_130 [post_translationally_modified_region] Paxillin
AEPSPTV
>PAXI_HUMAN_137_137 [post_translationally_modified_region] Paxillin
MSTSLGS
>PAXI_HUMAN_244_244 [post_translationally_modified_region] Paxillin
EMSSPQR
>PAXI_HUMAN_303_303 [post_translationally_modified_region] Paxillin
GRSSPGG
>UBR5_RAT_100_100 [post_translationally_modified_region] E3 ubiquitin-protein ligase UBR5 (6.3.2.-) (100 kDa protein) (E3 ubiquitin-protein ligase, HECT domain-containing 1) (Hyperplastic discs protein homolog)
TSDSPWF
>UBR5_RAT_342_342 [post_translationally_modified_region] E3 ubiquitin-protein ligase UBR5 (6.3.2.-) (100 kDa protein) (E3 ubiquitin-protein ligase, HECT domain-containing 1) (Hyperplastic discs protein homolog)
PVQSPVS
>UBR5_RAT_567_567 [post_translationally_modified_region] E3 ubiquitin-protein ligase UBR5 (6.3.2.-) (100 kDa protein) (E3 ubiquitin-protein ligase, HECT domain-containing 1) (Hyperplastic discs protein homolog)
QLRSPES
>UBR5_RAT_601_601 [post_translationally_modified_region] E3 ubiquitin-protein ligase UBR5 (6.3.2.-) (100 kDa protein) (E3 ubiquitin-protein ligase, HECT domain-containing 1) (Hyperplastic discs protein homolog)
PPPSPAS
>UBR5_RAT_797_797 [post_translationally_modified_region] E3 ubiquitin-protein ligase UBR5 (6.3.2.-) (100 kDa protein) (E3 ubiquitin-protein ligase, HECT domain-containing 1) (Hyperplastic discs protein homolog)
GQESPII
>UBR5_RAT_917_917 [post_translationally_modified_region] E3 ubiquitin-protein ligase UBR5 (6.3.2.-) (100 kDa protein) (E3 ubiquitin-protein ligase, HECT domain-containing 1) (Hyperplastic discs protein homolog)
ACVSVCF
>UBR5_RAT_1007_1007 [post_translationally_modified_region] E3 ubiquitin-protein ligase UBR5 (6.3.2.-) (100 kDa protein) (E3 ubiquitin-protein ligase, HECT domain-containing 1) (Hyperplastic discs protein homolog)
DPVSPPI
>UBR5_RAT_1216_1216 [post_translationally_modified_region] E3 ubiquitin-protein ligase UBR5 (6.3.2.-) (100 kDa protein) (E3 ubiquitin-protein ligase, HECT domain-containing 1) (Hyperplastic discs protein homolog)
KRTSPTA
>UBR5_RAT_1344_1344 [post_translationally_modified_region] E3 ubiquitin-protein ligase UBR5 (6.3.2.-) (100 kDa protein) (E3 ubiquitin-protein ligase, HECT domain-containing 1) (Hyperplastic discs protein homolog)
DPLSASS
>UBR5_RAT_1364_1364 [post_translationally_modified_region] E3 ubiquitin-protein ligase UBR5 (6.3.2.-) (100 kDa protein) (E3 ubiquitin-protein ligase, HECT domain-containing 1) (Hyperplastic discs protein homolog)
NQQSGTI
>UBR5_RAT_1470_1470 [post_translationally_modified_region] E3 ubiquitin-protein ligase UBR5 (6.3.2.-) (100 kDa protein) (E3 ubiquitin-protein ligase, HECT domain-containing 1) (Hyperplastic discs protein homolog)
VAESLIV
>UBR5_RAT_1730_1730 [post_translationally_modified_region] E3 ubiquitin-protein ligase UBR5 (6.3.2.-) (100 kDa protein) (E3 ubiquitin-protein ligase, HECT domain-containing 1) (Hyperplastic discs protein homolog)
AASSAGL
>UBR5_RAT_1769_1769 [post_translationally_modified_region] E3 ubiquitin-protein ligase UBR5 (6.3.2.-) (100 kDa protein) (E3 ubiquitin-protein ligase, HECT domain-containing 1) (Hyperplastic discs protein homolog)
YLTSASS
>UBR5_RAT_2016_2016 [post_translationally_modified_region] E3 ubiquitin-protein ligase UBR5 (6.3.2.-) (100 kDa protein) (E3 ubiquitin-protein ligase, HECT domain-containing 1) (Hyperplastic discs protein homolog)
FRRSDSM
>UBR5_RAT_2018_2018 [post_translationally_modified_region] E3 ubiquitin-protein ligase UBR5 (6.3.2.-) (100 kDa protein) (E3 ubiquitin-protein ligase, HECT domain-containing 1) (Hyperplastic discs protein homolog)
RSDSMTF
>UBR5_RAT_2061_2061 [post_translationally_modified_region] E3 ubiquitin-protein ligase UBR5 (6.3.2.-) (100 kDa protein) (E3 ubiquitin-protein ligase, HECT domain-containing 1) (Hyperplastic discs protein homolog)
GRPSQGL
>UBR5_RAT_2066_2066 [post_translationally_modified_region] E3 ubiquitin-protein ligase UBR5 (6.3.2.-) (100 kDa protein) (E3 ubiquitin-protein ligase, HECT domain-containing 1) (Hyperplastic discs protein homolog)
GLYSSSA
>UBR5_RAT_2279_2279 [post_translationally_modified_region] E3 ubiquitin-protein ligase UBR5 (6.3.2.-) (100 kDa protein) (E3 ubiquitin-protein ligase, HECT domain-containing 1) (Hyperplastic discs protein homolog)
GEGSGVA
>UBR5_RAT_2473_2473 [post_translationally_modified_region] E3 ubiquitin-protein ligase UBR5 (6.3.2.-) (100 kDa protein) (E3 ubiquitin-protein ligase, HECT domain-containing 1) (Hyperplastic discs protein homolog)
HGSSRSV
>UBR5_RAT_2475_2475 [post_translationally_modified_region] E3 ubiquitin-protein ligase UBR5 (6.3.2.-) (100 kDa protein) (E3 ubiquitin-protein ligase, HECT domain-containing 1) (Hyperplastic discs protein homolog)
SSRSVVD
</pre>
</td></tr></table><p>
<p>
The sequences of the specified features are written out.
<p>
The ID name of the sequence is formed from the original sequence name
with the start and end positions of the feature appended to it. So if
the feature came from a sequence with an ID name of 'XYZ' from positions
10 to 22, then the resulting ID name of the feature sequence will be
'XYZ_10_22'
<p>
The name of the type of feature is added to the start of the description
of the sequence in brackets, e.g.: '[exon]'.
<p>
The sequence is written out as a normal sequence.
<p>
If the feature is in the reverse sense of a nucleic acid sequence, then
it is reverse-complemented before being written.
<H2>
Data files
</H2>
None.
<H2>
Notes
</H2>
<p>Bear in mind that database annotation cannot always be trusted to
be reliable. If you rely upon annotation written by other people or
another program and do not independently verify such annotation, then
there is a chance that some of the reported features will be
erreneous.</p>
<h3>Controlling the output</h3>
<p>There are many options to control exactly what parts of the feature
table are written to file.</p>
<p>By default every feature in the feature table is
extracted. <tt>-type</tt> will set the specific feature type to
extract. See <a href="http://www.ebi.ac.uk/embl/WebFeindex.html">
http://www.ebi.ac.uk/embl/WebFeindex.html</a> for a list of the EMBL
feature types and see Appendix A of the Swissprot user manual
in <a href="http://www.uniprot.org/manual/sequence_annotation">
http://www.uniprot.org/manual/sequence_annotation</a> for a list of
the Swissprot feature types.</p>
<p>By default any feature tag in the feature table is
extracted. <tt>-tag</tt> specifies a feature tag reuired in any
feature extracted. Tags are the types of extra values that a feature
may have. For example in the EMBL feature table, a 'CDS' type of
feature may have the
tags <tt>/codon</tt>, <tt>/codon_start</tt>, <tt>/db_xref</tt>,
<tt>/EC_number</tt>, <tt>/evidence</tt>, <tt>/exception</tt>,
<tt>/function</tt>, <tt>/gene</tt>, <tt>/label</tt>, <tt>/map</tt>,
<tt>/note</tt>, <tt>/number</tt>, <tt>/partial</tt>, <tt>/product</tt>,
<tt>/protein_id</tt>,<tt>/pseudo</tt>, <tt>/standard_name</tt>,
<tt>/translation</tt>, <tt>/transl_except</tt>, <tt>/transl_table</tt>,
or <tt>/usedin</tt>. Some of these tags also have values, for
example <tt>/gene</tt> can have the value of the gene name.</p>
<p>By default any feature tag value in the feature table is shown. You
can set this using <tt>-tag</tt> to match any specific feature tag value you
wish to show. Tag values are the values associated with a feature tag,
for example <tt>/gene</tt> can have the value of the gene name. Bear
in mind only some of these tags can have values.</p>
<p>By default any feature source in the feature table is
shown. <tt>-source</tt> is used to set this to match a specific feature
source.</p>
<p>By default features in either sense are extracted. The -sense
option specifies a particular sense.</p>
<p>The minimum and maximum score of features to be reported may be
specified with -minscore and -maxscore.</p>
<p>To aid you in identifying the type of feature that has been output,
the type of feature is added to the start of the description of the
output sequence. Sometimes the description of a sequence is lost in
subsequent processing of the sequences file, so it is useful for the
type to be a part of the output sequence ID name. The -featinname
option specifies this behaviour. </p>
<p>To aid you in identifying the properties of a feature that has been
output, -describe specifies one or more tag names that will be added
to the output sequence "Description" text, together with their values
(if any). For example, if this is set to be <tt>gene</tt>, then if any
output feature has the tag (for example) <tt>/gene=BRCA1</tt>
associated with it, then the text <tt>(gene=BRCA1)</tt> will be added
to the Description line. By default no feature tag is given in the
"Description" text. You can set -describe to specify any feature tag
you wish to show.</p>
<h3> "Joined" features </h3>
<p>Some features, such as CDS (coding sequence) and mRNA are composed
of introns concatenated together. There may be other forms of 'joined'
sequence, depending on the feature table. By default, 'joined'
features are extracted as individual sequences. If the -join option
is specified, then any group of these features will be output as a
single sequence. If the <tt>-before</tt> and <tt>-after</tt>
qualifiers have been set, then only the sequence before the first
feature and after the last feature are added.</p>
<H2>
References
</H2>
None.
<H2>
Warnings
</H2>
<H2>
Diagnostic Error Messages
</H2>
If the end position of the sequence to be written is less than the start
position, then the warning message "Extraction region end less than
start for feature type [start-end] in ID name" is written and no
sequence is output.
<H2>
Exit status
</H2>
It always exits with status 0.
<H2>
Known bugs
</H2>
None.
<h2><a name="See also">See also</a></h2>
<table border cellpadding=4 bgcolor="#FFFFF0">
<tr><th>Program name</th>
<th>Description</th></tr>
<tr>
<td><a href="aligncopy.html">aligncopy</a></td>
<td>Read and write alignments</td>
</tr>
<tr>
<td><a href="aligncopypair.html">aligncopypair</a></td>
<td>Read and write pairs from alignments</td>
</tr>
<tr>
<td><a href="biosed.html">biosed</a></td>
<td>Replace or delete sequence sections</td>
</tr>
<tr>
<td><a href="codcopy.html">codcopy</a></td>
<td>Copy and reformat a codon usage table</td>
</tr>
<tr>
<td><a href="cutseq.html">cutseq</a></td>
<td>Remove a section from a sequence</td>
</tr>
<tr>
<td><a href="degapseq.html">degapseq</a></td>
<td>Remove non-alphabetic (e.g. gap) characters from sequences</td>
</tr>
<tr>
<td><a href="descseq.html">descseq</a></td>
<td>Alter the name or description of a sequence</td>
</tr>
<tr>
<td><a href="entret.html">entret</a></td>
<td>Retrieve sequence entries from flatfile databases and files</td>
</tr>
<tr>
<td><a href="extractalign.html">extractalign</a></td>
<td>Extract regions from a sequence alignment</td>
</tr>
<tr>
<td><a href="extractseq.html">extractseq</a></td>
<td>Extract regions from a sequence</td>
</tr>
<tr>
<td><a href="featcopy.html">featcopy</a></td>
<td>Read and write a feature table</td>
</tr>
<tr>
<td><a href="featmerge.html">featmerge</a></td>
<td>Merge two overlapping feature tables</td>
</tr>
<tr>
<td><a href="featreport.html">featreport</a></td>
<td>Read and write a feature table</td>
</tr>
<tr>
<td><a href="feattext.html">feattext</a></td>
<td>Return a feature table original text</td>
</tr>
<tr>
<td><a href="listor.html">listor</a></td>
<td>Write a list file of the logical OR of two sets of sequences</td>
</tr>
<tr>
<td><a href="makenucseq.html">makenucseq</a></td>
<td>Create random nucleotide sequences</td>
</tr>
<tr>
<td><a href="makeprotseq.html">makeprotseq</a></td>
<td>Create random protein sequences</td>
</tr>
<tr>
<td><a href="maskambignuc.html">maskambignuc</a></td>
<td>Mask all ambiguity characters in nucleotide sequences with N</td>
</tr>
<tr>
<td><a href="maskambigprot.html">maskambigprot</a></td>
<td>Mask all ambiguity characters in protein sequences with X</td>
</tr>
<tr>
<td><a href="maskfeat.html">maskfeat</a></td>
<td>Write a sequence with masked features</td>
</tr>
<tr>
<td><a href="maskseq.html">maskseq</a></td>
<td>Write a sequence with masked regions</td>
</tr>
<tr>
<td><a href="newseq.html">newseq</a></td>
<td>Create a sequence file from a typed-in sequence</td>
</tr>
<tr>
<td><a href="nohtml.html">nohtml</a></td>
<td>Remove mark-up (e.g. HTML tags) from an ASCII text file</td>
</tr>
<tr>
<td><a href="noreturn.html">noreturn</a></td>
<td>Remove carriage return from ASCII files</td>
</tr>
<tr>
<td><a href="nospace.html">nospace</a></td>
<td>Remove whitespace from an ASCII text file</td>
</tr>
<tr>
<td><a href="notab.html">notab</a></td>
<td>Replace tabs with spaces in an ASCII text file</td>
</tr>
<tr>
<td><a href="notseq.html">notseq</a></td>
<td>Write to file a subset of an input stream of sequences</td>
</tr>
<tr>
<td><a href="nthseq.html">nthseq</a></td>
<td>Write to file a single sequence from an input stream of sequences</td>
</tr>
<tr>
<td><a href="nthseqset.html">nthseqset</a></td>
<td>Read and write (return) one set of sequences from many</td>
</tr>
<tr>
<td><a href="pasteseq.html">pasteseq</a></td>
<td>Insert one sequence into another</td>
</tr>
<tr>
<td><a href="revseq.html">revseq</a></td>
<td>Reverse and complement a nucleotide sequence</td>
</tr>
<tr>
<td><a href="seqcount.html">seqcount</a></td>
<td>Read and count sequences</td>
</tr>
<tr>
<td><a href="seqret.html">seqret</a></td>
<td>Read and write (return) sequences</td>
</tr>
<tr>
<td><a href="seqretsetall.html">seqretsetall</a></td>
<td>Read and write (return) many sets of sequences</td>
</tr>
<tr>
<td><a href="seqretsplit.html">seqretsplit</a></td>
<td>Read sequences and write them to individual files</td>
</tr>
<tr>
<td><a href="showfeat.html">showfeat</a></td>
<td>Display features of a sequence in pretty format</td>
</tr>
<tr>
<td><a href="sizeseq.html">sizeseq</a></td>
<td>Sort sequences by size</td>
</tr>
<tr>
<td><a href="skipredundant.html">skipredundant</a></td>
<td>Remove redundant sequences from an input set</td>
</tr>
<tr>
<td><a href="skipseq.html">skipseq</a></td>
<td>Read and write (return) sequences, skipping first few</td>
</tr>
<tr>
<td><a href="splitsource.html">splitsource</a></td>
<td>Split sequence(s) into original source sequences</td>
</tr>
<tr>
<td><a href="splitter.html">splitter</a></td>
<td>Split sequence(s) into smaller sequences</td>
</tr>
<tr>
<td><a href="trimest.html">trimest</a></td>
<td>Remove poly-A tails from nucleotide sequences</td>
</tr>
<tr>
<td><a href="trimseq.html">trimseq</a></td>
<td>Remove unwanted characters from start and end of sequence(s)</td>
</tr>
<tr>
<td><a href="trimspace.html">trimspace</a></td>
<td>Remove extra whitespace from an ASCII text file</td>
</tr>
<tr>
<td><a href="twofeat.html">twofeat</a></td>
<td>Find neighbouring pairs of features in sequence(s)</td>
</tr>
<tr>
<td><a href="union.html">union</a></td>
<td>Concatenate multiple sequences into a single sequence</td>
</tr>
<tr>
<td><a href="vectorstrip.html">vectorstrip</a></td>
<td>Remove vectors from the ends of nucleotide sequence(s)</td>
</tr>
<tr>
<td><a href="yank.html">yank</a></td>
<td>Add a sequence reference (a full USA) to a list file</td>
</tr>
</table>
<H2>
Author(s)
</H2>
Gary Williams formerly at:
<br>
MRC Rosalind Franklin Centre for Genomics Research
Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SB, UK
<p>
Please report all bugs to the EMBOSS bug team (emboss-bug © emboss.open-bio.org) not to the original author.
<H2>
History
</H2>
Written (Dec 12 2001) - Gary Williams
<p>
Added '-join' parameter (June 2002) - Gary Williams
<H2>
Target users
</H2>
This program is intended to be used by everyone and everything, from naive users to embedded scripts.
<H2>
Comments
</H2>
None
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