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splitsource
Wiki
The master copies of EMBOSS documentation are available at
http://emboss.open-bio.org/wiki/Appdocs on the EMBOSS Wiki.
Please help by correcting and extending the Wiki pages.
Function
Split sequence(s) into original source sequences
Description
splitsource splits a synthetic sequence into original source sequences
by processing the "source" features in the feature table.
Algorithm
The "source" feature annotated the origin of a sequence region.
Synthetic sequences are characterized by having two or more "source"
features each describing one of the original sequence fragments.
Usage
Here is a sample session with splitsource
Split a sequence into original subsequences
% splitsource ena:A01139
Split sequence(s) into original source sequences
output sequence(s) [a01139.fasta]:
Go to the input files for this example
Go to the output files for this example
Command line arguments
Split sequence(s) into original source sequences
Version: EMBOSS:6.6.0.0
Standard (Mandatory) qualifiers:
[-sequence] seqall Sequence(s) filename and optional format, or
reference (input USA)
[-outseq] seqoutall [.] Sequence set(s)
filename and optional format (output USA)
Additional (Optional) qualifiers: (none)
Advanced (Unprompted) qualifiers:
-feature boolean [N] Retain feature information
Associated qualifiers:
"-sequence" associated qualifiers
-sbegin1 integer Start of each sequence to be used
-send1 integer End of each sequence to be used
-sreverse1 boolean Reverse (if DNA)
-sask1 boolean Ask for begin/end/reverse
-snucleotide1 boolean Sequence is nucleotide
-sprotein1 boolean Sequence is protein
-slower1 boolean Make lower case
-supper1 boolean Make upper case
-scircular1 boolean Sequence is circular
-squick1 boolean Read id and sequence only
-sformat1 string Input sequence format
-iquery1 string Input query fields or ID list
-ioffset1 integer Input start position offset
-sdbname1 string Database name
-sid1 string Entryname
-ufo1 string UFO features
-fformat1 string Features format
-fopenfile1 string Features file name
"-outseq" associated qualifiers
-osformat2 string Output seq format
-osextension2 string File name extension
-osname2 string Base file name
-osdirectory2 string Output directory
-osdbname2 string Database name to add
-ossingle2 boolean Separate file for each entry
-oufo2 string UFO features
-offormat2 string Features format
-ofname2 string Features file name
-ofdirectory2 string Output directory
General qualifiers:
-auto boolean Turn off prompts
-stdout boolean Write first file to standard output
-filter boolean Read first file from standard input, write
first file to standard output
-options boolean Prompt for standard and additional values
-debug boolean Write debug output to program.dbg
-verbose boolean Report some/full command line options
-help boolean Report command line options and exit. More
information on associated and general
qualifiers can be found with -help -verbose
-warning boolean Report warnings
-error boolean Report errors
-fatal boolean Report fatal errors
-die boolean Report dying program messages
-version boolean Report version number and exit
Input file format
The input is a standard EMBOSS sequence query (also known as a 'USA').
Major sequence database sources defined as standard in EMBOSS
installations include srs:embl, srs:uniprot and ensembl
Data can also be read from sequence output in any supported format
written by an EMBOSS or third-party application.
The input format can be specified by using the command-line qualifier
-sformat xxx, where 'xxx' is replaced by the name of the required
format. The available format names are: gff (gff3), gff2, embl (em),
genbank (gb, refseq), ddbj, refseqp, pir (nbrf), swissprot (swiss, sw),
dasgff and debug.
See: http://emboss.sf.net/docs/themes/SequenceFormats.html for further
information on sequence formats.
Input files for usage example
Database entry: ena:A01139
ID A01139; SV 1; linear; unassigned DNA; PAT; PRO; 279 BP.
XX
AC A01139;
XX
DT 16-MAR-1994 (Rel. 38, Created)
DT 14-FEB-2001 (Rel. 66, Last updated, Version 5)
XX
DE Fusion DNA (L.lactis MSP signal sequence and H.medicinalis desulfatohirudin
DE coding sequence)
XX
KW .
XX
OS Lactococcus lactis
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Lactococcus.
XX
OS Hirudo medicinalis (medicinal leech)
OC Eukaryota; Metazoa; Lophotrochozoa; Annelida; Clitellata; Hirudinida;
OC Hirudinea; Arhynchobdellida; Hirudiniformes; Hirudinidae; Hirudo.
XX
RN [1]
RP 1-279
RA Suri B., Schmitz A.;
RT "Bacterial vectors.";
RL Patent number EP0449770-A2/2, 02-OCT-1991.
RL CIBA-GEIGY AG.
XX
FH Key Location/Qualifiers
FH
FT source 1..81
FT /organism="Lactococcus lactis"
FT /focus
FT /strain="LM0230"
FT /mol_type="unassigned DNA"
FT /db_xref="taxon:1358"
FT source 82..279
FT /organism="Hirudo medicinalis"
FT /mol_type="unassigned DNA"
FT /db_xref="taxon:6421"
FT CDS 1..279
FT /transl_table=11
FT /note="fusion of MSP signal peptide and hirudin"
FT /protein_id="CAA00134.1"
FT /translation="MKKKIISAILMSTVILSAAAPLSGVYAVVYTDCTESGQNLCLCEG
FT SNVCGQGNKCILGSDGEKNQCVTGEGTPKPQSHNDGDFEEIPEEYLQ"
FT sig_peptide 1..81
FT /product="MSP signal peptide"
FT mat_peptide 82..279
FT /product="desulfatohirudin"
XX
SQ Sequence 279 BP; 76 A; 66 C; 68 G; 69 T; 0 other;
atgaaaaaaa agattatctc agctatttta atgtctacag tgatactttc tgctgcagcc 60
ccgttgtcag gtgtttacgc tgttgtttac accgactgca ccgaatctgg tcagaacctg 120
tgcctgtgcg aaggttctaa cgtttgcggt cagggtaaca aatgcatcct gggttctgac 180
ggtgaaaaaa accagtgcgt taccggcgaa ggtaccccga aaccgcagtc tcacaacgac 240
ggtgacttcg aagaaatccc ggaagaatac ctgcagtag 279
//
Output file format
The output is a standard EMBOSS sequence file.
The results can be output in one of several styles by using the
command-line qualifier -osformat xxx, where 'xxx' is replaced by the
name of the required format. The available format names are: embl,
genbank, gff, pir, swiss, dasgff, debug, listfile, dbmotif, diffseq,
excel, feattable, motif, nametable, regions, seqtable, simple, srs,
table, tagseq.
See: http://emboss.sf.net/docs/themes/SequenceFormats.html for further
information on sequence formats.
Output files for usage example
File: a01139.fasta
>A01139_1_1-81 organism="Lactococcus lactis" strain="LM0230" Fusion DNA (L.lacti
s MSP signal sequence and H.medicinalis desulfatohirudin coding sequence)
atgaaaaaaaagattatctcagctattttaatgtctacagtgatactttctgctgcagcc
ccgttgtcaggtgtttacgct
>A01139_2_82-279 organism="Hirudo medicinalis" Fusion DNA (L.lactis MSP signal s
equence and H.medicinalis desulfatohirudin coding sequence)
gttgtttacaccgactgcaccgaatctggtcagaacctgtgcctgtgcgaaggttctaac
gtttgcggtcagggtaacaaatgcatcctgggttctgacggtgaaaaaaaccagtgcgtt
accggcgaaggtaccccgaaaccgcagtctcacaacgacggtgacttcgaagaaatcccg
gaagaatacctgcagtag
Data files
None.
Notes
None.
References
None.
Warnings
None.
Diagnostic Error Messages
None.
Exit status
It always exits with status 0.
Known bugs
None.
See also
Program name Description
aligncopy Read and write alignments
aligncopypair Read and write pairs from alignments
biosed Replace or delete sequence sections
codcopy Copy and reformat a codon usage table
cutseq Remove a section from a sequence
degapseq Remove non-alphabetic (e.g. gap) characters from sequences
descseq Alter the name or description of a sequence
entret Retrieve sequence entries from flatfile databases and files
extractalign Extract regions from a sequence alignment
extractfeat Extract features from sequence(s)
extractseq Extract regions from a sequence
featcopy Read and write a feature table
featmerge Merge two overlapping feature tables
featreport Read and write a feature table
feattext Return a feature table original text
listor Write a list file of the logical OR of two sets of sequences
makenucseq Create random nucleotide sequences
makeprotseq Create random protein sequences
maskambignuc Mask all ambiguity characters in nucleotide sequences with
N
maskambigprot Mask all ambiguity characters in protein sequences with X
maskfeat Write a sequence with masked features
maskseq Write a sequence with masked regions
newseq Create a sequence file from a typed-in sequence
nohtml Remove mark-up (e.g. HTML tags) from an ASCII text file
noreturn Remove carriage return from ASCII files
nospace Remove whitespace from an ASCII text file
notab Replace tabs with spaces in an ASCII text file
notseq Write to file a subset of an input stream of sequences
nthseq Write to file a single sequence from an input stream of
sequences
nthseqset Read and write (return) one set of sequences from many
pasteseq Insert one sequence into another
revseq Reverse and complement a nucleotide sequence
seqcount Read and count sequences
seqret Read and write (return) sequences
seqretsetall Read and write (return) many sets of sequences
seqretsplit Read sequences and write them to individual files
sizeseq Sort sequences by size
skipredundant Remove redundant sequences from an input set
skipseq Read and write (return) sequences, skipping first few
splitter Split sequence(s) into smaller sequences
trimest Remove poly-A tails from nucleotide sequences
trimseq Remove unwanted characters from start and end of sequence(s)
trimspace Remove extra whitespace from an ASCII text file
union Concatenate multiple sequences into a single sequence
vectorstrip Remove vectors from the ends of nucleotide sequence(s)
yank Add a sequence reference (a full USA) to a list file
Author(s)
Peter Rice
European Bioinformatics Institute, Wellcome Trust Genome Campus,
Hinxton, Cambridge CB10 1SD, UK
Please report all bugs to the EMBOSS bug team
(emboss-bug (c) emboss.open-bio.org) not to the original author.
History
Target users
This program is intended to be used by everyone and everything, from
naive users to embedded scripts.
Comments
None
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