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>>>>H45989
ID H45989 standard; RNA; EST; 495 BP.
XX
AC H45989;
XX
SV H45989.1
XX
DT 18-NOV-1995 (Rel. 45, Created)
DT 04-MAR-2000 (Rel. 63, Last updated, Version 2)
XX
DE yo13c02.s1 Soares adult brain N2b5HB55Y Homo sapiens cDNA clone
DE IMAGE:177794 3', mRNA sequence.
XX
KW EST.
XX
OS Homo sapiens (human)
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Teleostomi;
OC Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
XX
RN [1]
RP 1-495
RA Hillier L., Clark N., Dubuque T., Elliston K., Hawkins M., Holman M.,
RA Hultman M., Kucaba T., Le M., Lennon G., Marra M., Parsons J., Rifkin L.,
RA Rohlfing T., Soares M., Tan F., Trevaskis E., Waterston R., Williamson A.,
RA Wohldmann P., Wilson R.;
RT "The WashU-Merck EST Project";
RL Unpublished.
XX
DR RZPD; IMAGp998F03326; IMAGp998F03326.
XX
CC On May 8, 1995 this sequence version replaced gi:800819.
CC Contact: Wilson RK
CC Washington University School of Medicine
CC 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
CC Tel: 314 286 1800
CC Fax: 314 286 1810
CC Email: est@watson.wustl.edu
CC Insert Size: 544
CC High quality sequence stops: 265
CC Source: IMAGE Consortium, LLNL
CC This clone is available royalty-free through LLNL ; contact the
CC IMAGE Consortium (info@image.llnl.gov) for further information.
CC Possible reversed clone: polyT not found
CC Insert Length: 544 Std Error: 0.00
CC Seq primer: SP6
CC High quality sequence stop: 265.
XX
FH Key Location/Qualifiers
FH
FT source 1. .495
FT /db_xref="taxon:9606"
FT /db_xref="ESTLIB:300"
FT /db_xref="RZPD:IMAGp998F03326"
FT /note="Organ: brain; Vector: pT7T3D (Pharmacia) with a
FT modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
FT strand cDNA was primed with a Not I - oligo(dT) primer [5'
FT TGTTACCAATCTGAAGTGGGAGCGGCCGCGCTTTTTTTTTTTTTTTTTTT 3'],
FT double-stranded cDNA was size selected, ligated to Eco RI
FT adapters (Pharmacia), digested with Not I and cloned into
FT the Not I and Eco RI sites of a modified pT7T3 vector
FT (Pharmacia). Library went through one round of
FT normalization to a Cot = 53. Library constructed by Bento
FT Soares and M.Fatima Bonaldo. The adult brain RNA was
FT provided by Dr. Donald H. Gilden. Tissue was acquired 17-18
FT hours after death which occurred in consequence of a
FT ruptured aortic aneurysm. RNA was prepared from a pool of
FT tissues representing the following areas of the brain:
FT frontal, parietal, temporal and occipital cortex from the
FT left and right hemispheres, subcortical white matter, basal
FT ganglia, thalamus, cerebellum, midbrain, pons and medulla."
FT /sex="Male"
FT /organism="Homo sapiens"
FT /clone="IMAGE:177794"
FT /clone_lib="Soares adult brain N2b5HB55Y"
FT /dev_stage="55-year old"
FT /lab_host="DH10B (ampicillin resistant)"
XX
SQ Sequence 495 BP; 73 A; 135 C; 169 G; 104 T; 14 other;
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