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#!/usr/bin/env python
#
# Part of: https://github.com/mateidavid/fast5
#
# (c) 2017: Matei David, Ontario Institute for Cancer Research
# MIT License
#
import argparse
import logging
import os
import sys
import fast5
import signal
signal.signal(signal.SIGPIPE, signal.SIG_DFL)
policy_d = {
"drop": 0,
"pack": 1,
"unpack": 2,
"copy": 3,
}
def add_fast5(fn, rel_dn, args):
logger.info("adding fast5 fn=" + fn + " rel_dn=" + rel_dn)
return [(fn, os.path.normpath(os.path.join(args.output, rel_dn, os.path.basename(fn))))]
def add_dir(dn, args):
l = list()
logger.info("processing dir dn=" + dn)
for t in os.walk(dn):
rel_dn = os.path.relpath(t[0], dn)
for rel_fn in t[2]:
fn = os.path.join(t[0], rel_fn)
if fast5.File.is_valid_file(fn):
l += add_fast5(fn, rel_dn, args)
if not args.recurse:
break
return l
def add_fofn(fn, args):
l = list()
logger.info("processing fofn fn=" + fn)
if fn != "-":
f = open(fn)
else:
f = sys.stdin
for p in f:
p = p.strip()
if fast5.File.is_valid_file(p):
l += add_fast5(p, "", args)
else:
logger.warning("fofn line not a fast5 file: " + p)
if fn != "-":
f.close()
return l
def add_paths(pl, args):
l = list()
if len(pl) == 0:
pl.append("-")
for p in pl:
if os.path.isdir(p):
l += add_dir(p, args)
elif fast5.File.is_valid_file(p):
l += add_fast5(p, "", args)
else:
l += add_fofn(p, args)
return l
if __name__ == "__main__":
description = """
Pack and unpack ONT fast5 files.
"""
parser = argparse.ArgumentParser(description=description, epilog="")
parser.add_argument("--log", default="warning",
help="log level")
#
parser.add_argument("--pack", action="store_true",
help="Pack data (default).")
parser.add_argument("--unpack", action="store_true",
help="Unpack data.")
parser.add_argument("--archive", action="store_true",
help="Pack raw samples data, drop rest.")
parser.add_argument("--fastq", action="store_true",
help="Pack fastq data, drop rest.")
#
parser.add_argument("--rs", choices=["drop", "pack", "unpack", "copy"],
help="Policy for raw samples.")
parser.add_argument("--ed", choices=["drop", "pack", "unpack", "copy"],
help="Policy for eventdetection events.")
parser.add_argument("--fq", choices=["drop", "pack", "unpack", "copy"],
help="Policy for fastq.")
parser.add_argument("--ev", choices=["drop", "pack", "unpack", "copy"],
help="Policy for basecall events.")
parser.add_argument("--al", choices=["drop", "pack", "unpack", "copy"],
help="Policy for basecall alignment.")
#
parser.add_argument("--force", action="store_true",
help="Overwrite existing destination files.")
parser.add_argument("--qv-bits", type=int,
help="QV bits to keep.")
parser.add_argument("--p-model-state-bits", type=int,
help="p_model_state bits to keep.")
parser.add_argument("-R", "--recurse", action="store_true",
help="Recurse in input directories.")
parser.add_argument("-o", "--output", required=True,
help="Output directory.")
#
parser.add_argument("inputs", nargs="*", default=[], action="append",
help="Input directories, fast5 files, or files of fast5 file names. For input directories, the subdirectory hierarchy (if traversed with --recurse) is recreated in the output directory.")
args = parser.parse_args()
numeric_log_level = getattr(logging, args.log.upper(), None)
if not isinstance(numeric_log_level, int):
raise ValueError("Invalid log level: '%s'" % args.log)
logging.basicConfig(level=numeric_log_level,
format="%(asctime)s %(name)s.%(levelname)s %(message)s",
datefmt="%Y/%m/%d %H:%M:%S")
logger = logging.getLogger(os.path.basename(__file__))
fast5.Logger.set_levels_from_options([args.log.lower()])
logger.debug("args: " + str(args))
if args.pack + args.unpack + args.archive + args.fastq > 1:
sys.exit("At most one of --pack/--unpack/--archive/--fastq may be specified")
if (not args.pack and
not args.unpack and
not args.archive and
not args.fastq and
args.rs is None and
args.ed is None and
args.fq is None and
args.ev is None and
args.al is None):
args.pack = True
if args.pack:
if args.rs is None: args.rs = "pack"
if args.ed is None: args.ed = "pack"
if args.fq is None: args.fq = "pack"
if args.ev is None: args.ev = "pack"
if args.al is None: args.al = "pack"
if args.unpack:
if args.rs is None: args.rs = "unpack"
if args.ed is None: args.ed = "unpack"
if args.fq is None: args.fq = "unpack"
if args.ev is None: args.ev = "unpack"
if args.al is None: args.al = "unpack"
if args.archive:
if args.rs is None: args.rs = "pack"
if args.ed is None: args.ed = "drop"
if args.fq is None: args.fq = "drop"
if args.ev is None: args.ev = "drop"
if args.al is None: args.al = "drop"
if args.fastq:
if args.rs is None: args.rs = "drop"
if args.ed is None: args.ed = "drop"
if args.fq is None: args.fq = "pack"
if args.ev is None: args.ev = "drop"
if args.al is None: args.al = "drop"
if args.rs is None: args.rs = "drop"
if args.ed is None: args.ed = "drop"
if args.fq is None: args.fq = "drop"
if args.ev is None: args.ev = "drop"
if args.al is None: args.al = "drop"
logger.info("rs: " + args.rs)
logger.info("ed: " + args.ed)
logger.info("fq: " + args.fq)
logger.info("ev: " + args.ev)
logger.info("al: " + args.al)
fp = fast5.File_Packer(
policy_d[args.rs],
policy_d[args.ed],
policy_d[args.fq],
policy_d[args.ev],
policy_d[args.al],
)
if args.force: fp.set_force(True)
if args.qv_bits: fp.set_qv_bits(args.qv_bits)
if args.p_model_state_bits: fp.set_p_model_state_bits(args.p_model_state_bits)
fl = add_paths(args.inputs[0], args)
errored_files_cnt = 0
input_bytes = 0
output_bytes = 0
for t in fl:
ifn = t[0]
ofn = t[1]
odn = os.path.dirname(t[1])
if not os.path.isdir(odn):
os.makedirs(odn)
logger.info("packing ifn=" + ifn + " ofn=" + ofn)
try:
fp.run(ifn, ofn)
except RuntimeError as e:
logger.warning("error packing " + ifn + ": " + str(e))
os.remove(ofn)
errored_files_cnt += 1
continue
input_bytes += os.stat(ifn).st_size
output_bytes += os.stat(ofn).st_size
cnt = fp.get_counts()
cnt_total_bits = dict()
output_ds_bytes = 0
print("bp_seq_count\t%d" % cnt["bp_seq_count"])
if cnt["bp_seq_count"] == 0:
cnt["bp_seq_count"] = float('nan')
for cl in [["rs_count", "rs_bits"],
["ed_count", "ed_skip_bits", "ed_len_bits"],
["fq_count", "fq_bp_bits", "fq_qv_bits"],
["ev_count", "ev_rel_skip_bits", "ev_skip_bits", "ev_len_bits", "ev_move_bits", "ev_p_model_state_bits"],
["al_count", "al_template_step_bits", "al_complement_step_bits", "al_move_bits"]]:
cnt_total_bits[cl[0]] = 0
if cnt[cl[0]] == 0:
continue
print(cl[0] + "\t%d" % cnt[cl[0]])
for c in cl[1:]:
cnt_total_bits[cl[0]] += cnt[c]
if cnt[c] == 0:
continue
print((c + "\t%d\t%.2f\t%.2f") % (cnt[c], float(cnt[c]) / cnt[cl[0]], float(cnt[c])/cnt["bp_seq_count"]))
output_ds_bytes += cnt_total_bits[cl[0]] / 8
print(cl[0].split('_')[0] + "_total_bits\t%d\t%.2f\t%.2f" % (cnt_total_bits[cl[0]], float(cnt_total_bits[cl[0]])/cnt[cl[0]], float(cnt_total_bits[cl[0]])/cnt["bp_seq_count"]))
if cnt["rs_total_duration"] > .001 and cnt["rs_called_duration"] > .001:
print("rs_total_duration\t%.2f" % cnt["rs_total_duration"])
print("rs_called_duration\t%.2f" % cnt["rs_called_duration"])
print("rs_frac_called\t%.2f" % (cnt["rs_called_duration"] / cnt["rs_total_duration"]))
print("bp_per_sec\t%.2f" % (float(cnt["bp_seq_count"]) / cnt["rs_called_duration"]))
print("input_bytes\t%d" % input_bytes)
print("output_bytes\t%d" % output_bytes)
print("output_overhead_bytes\t%d" % (output_bytes - output_ds_bytes))
print("processed_files\t%d" % len(fl))
if errored_files_cnt > 0:
print("errored_files\t%d" % errored_files_cnt)
sys.exit(errored_files_cnt > 0)
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