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#include "indelCoder.h"
#include "indelCoderUtils.h"
using namespace std;
/********************************************************************************************
run
*********************************************************************************************/
void indelCoder::run(){
startSequenceContainer(); // note: only Amino seq is implemented
readSequenceIntoGaps(); // Find and sort all gaps in MSA
printGapsInfo();
switch (indelCoderOptions::_codingType)
{
case (indelCoderOptions::SIC):
delimitationOfCharactersSIC();
break;
case (indelCoderOptions::MCIC):
LOGnOUT(2,<<endl<< "WARNING: The MCIC implementation is incomplete.\n Please re-run using SIC coding\n"<<endl);
return;
delimitationOfCharacters(indelCoderOptions::_codingType);
break;
case (indelCoderOptions::MCIC2):
LOGnOUT(2,<<endl<< "WARNING: The MCIC2 implementation is incomplete.\n Please re-run using SIC coding\n"<<endl);
return;
delimitationOfCharacters(indelCoderOptions::_codingType);
break;
default:
errorMsg::reportError("unknown type in codingType - {SIC, MCIC, MCIC2}");
}
//if(indelCoderOptions::_isMCIC2)
// delimitationOfCharactersMCIC2();
//else
// delimitationOfCharacters();
resizeMatrix(_matrix,_sc.numberOfSeqs(),_characters.size());
if(indelCoderOptions::_codingType==indelCoderOptions::SIC)
determinationCharacterStateSIC();
else{
determinationCharacterState();
determinationStepsMatrix();
}
//printCharacters(); //DEBUG
printFasta();
printNexus();
printIndelSummary(); // Tal's own format in which all indel information is provided.
}
/********************************************************************************************
printCharacters
*********************************************************************************************/
void indelCoder::printCharacters(){
for(int i = 0; i < _characters.size(); ++i)
{
cout<<"Character "<<_characters[i]->getCoord5()<<" "<<_characters[i]->getCoord3()<<" "<<_characters[i]->getNumOfGaps()<<" "<<_characters[i]->getNumOfStates()<<endl;
//_characters[i]->printScStates(); //DEBUG
_characters[i]->printStates();
_characters[i]->printStepsMatrix();
}
}
/********************************************************************************************
startSequenceContainer
*********************************************************************************************/
void indelCoder::startSequenceContainer(){
amino alph; // note: we can add parameter with Alphabet type
ifstream in(indelCoderOptions::_seqFile.c_str());
_sc = recognizeFormat::read(in,&alph);
_gapsVperSc.resize(_sc.numberOfSeqs());
LOGnOUT(4,<<"Seq "<<indelCoderOptions::_seqFile.c_str()<<endl);
LOGnOUT(4,<<"numberOfSeqs="<<_sc.numberOfSeqs()<<endl);
LOGnOUT(4,<<"seqLen="<<_sc.seqLen()<<endl);
}
/********************************************************************************************
readSequenceIntoGaps
// Sort the vector containing all indels by I =(i1,i2), K =(k1,k2), I<K iff i1<k1 or i1=k1 and i2<k2
*********************************************************************************************/
void indelCoder::readSequenceIntoGaps(){
LOGnOUT(4,<<endl<< "Step (1) readSequenceIntoGaps..."<<endl);
LOGnOUT(5,<< " All MSA gaps are sorted by coordinates"<<endl);
LOGnOUT(5,<< " Sort by: I =(i1,i2), K =(k1,k2), I<K iff i1<k1 or i1=k1 and i2<k2"<<endl);
int gapSign = -1;
int UnknownSign = _sc.getAlphabet()->unknown(); // Note that within amino class, 'X' is also coded as unknown
int coord5=0;
int coord3=0;
int seqID=0;
int coord5abs=0; //coord5MinusNumOfGapPositionsFromGenomeStart
for(int s=0; s<_sc.numberOfSeqs(); ++s){
cout<<_sc[s].id()<<" "<<_sc[s].name()<<"\n";
int numOfGapPositionsFromGenomeStart = 0;
int seqLength =_sc.seqLen();
for(int pos=0; pos<seqLength; ++pos){
if(_sc[s][pos] == gapSign){
coord5 = pos;
coord5abs = coord5-numOfGapPositionsFromGenomeStart;
++numOfGapPositionsFromGenomeStart;
while(pos<(seqLength-1) && _sc[s][pos+1] == gapSign){
++pos;
++numOfGapPositionsFromGenomeStart;
}
coord3 = pos;
seqID = _sc[s].id();
//cout<<"new gap found "<<seqID<<" "<<coord5<<" "<<coord3<<endl;
if(indelCoderOptions::_isOmitLeadingAndEndingGaps && (coord5abs==0 || coord3==seqLength-1)){
LOGnOUT(4,<< "Skip Leading/Ending Gap. seq="<< s<<" coord5="<<coord5abs<<" coord3="<<coord3<<endl);
_unknowns.insertNewGap(coord5, coord3,seqID, coord5abs);
}else{
_gaps.insertNewGap(coord5, coord3,seqID, coord5abs);
_gapsVperSc[seqID].insertNewGap(coord5, coord3,seqID, coord5abs); // used additionally were gaps are pre-sorted by seq
}
}
if(_sc[s][pos] == UnknownSign){
coord5 = pos;
coord5abs = coord5-numOfGapPositionsFromGenomeStart;
while(pos<(seqLength-1) && _sc[s][pos+1] == UnknownSign){
++pos;
}
coord3 = pos;
seqID = _sc[s].id();
_unknowns.insertNewGap(coord5, coord3,seqID, coord5abs);
}
}
}
LOGnOUT(4,<<endl<< "There are "<<_gaps.numOfGaps()<<" gaps"<<endl);
//_gaps.printGaps(); //DEBUG
}
/********************************************************************************************
delimitationOfCharacters
// 1) delimitation of the characters.
// Each character is a region of the alignment that is fully represented by one indel,
// and this indel is the longest one in these coordinates.
// Start with the first gap(indel) in the sorted vector to define the first character, following characters
// character_1 is defined by gap_1
// For i in gap_1:gap_N (N gaps in the sorted vector)
// while gap_i(3') < character_j(3')
// gap_i is within character_j
// else j++
*********************************************************************************************/
void indelCoder::delimitationOfCharacters(indelCoderOptions::codingType type){
LOGnOUT(4,<<endl<< "Step (2) delimitationOfCharacters... Complex Coding "<<indelCoderOptions::getCodingType(type)<<endl);
LOGnOUT(5,<< " Finding the required positions(=characters) in the coded sequence"<<endl);
LOGnOUT(5,<< " The number of characters <= the number of found gaps (each character may include several gaps)"<<endl);
if(type == indelCoderOptions::MCIC)
LOGnOUT(5,<< " gap_i is extending previous character if it's start is the same but ends further"<<endl);
if(type == indelCoderOptions::MCIC2)
LOGnOUT(5,<< " gap_i is extending previous character if it's start is included in the previous character but ends further"<<endl);
int i=0;
character* character_p = new character(_gaps[i]->getCoord5(), _gaps[i]->getCoord3(),_sc.numberOfSeqs());
_characters.push_back(character_p);
while( i<_gaps.numOfGaps())
{
// gap_i is included in previous character if it's start(5) & end(3) coord are within the start & end coord of the character
while( _gaps[i]->getCoord5()>=character_p->getCoord5() && _gaps[i]->getCoord3()<=character_p->getCoord3()){
character_p->addGap(_gaps[i],i+1);
i++;
if(i>=_gaps.numOfGaps())
break;
}
if(i>=_gaps.numOfGaps())
break;
bool condition;
if(type == indelCoderOptions::MCIC)
condition = _gaps[i]->getCoord5()==character_p->getCoord5(); // gap_i is extending previous character if it's start is the same but ends(3) further
if(type == indelCoderOptions::MCIC2)
condition = _gaps[i]->getCoord5()<=character_p->getCoord3(); // gap_i is extending previous character if it's start is included in the previous character but ends(3) further
while(condition && _gaps[i]->getCoord3()>character_p->getCoord3() ){
character_p->setCoord3(_gaps[i]->getCoord3());
character_p->addGap(_gaps[i],i+1);
i++;
if(i>=_gaps.numOfGaps())
break;
}
// new character is required for this gap
if(i<_gaps.numOfGaps() && _gaps[i]->getCoord5() > character_p->getCoord5() && _gaps[i]->getCoord3() > character_p->getCoord3()){
character_p = new character(_gaps[i]->getCoord5(), _gaps[i]->getCoord3(),_sc.numberOfSeqs());
_characters.push_back(character_p);
character_p->addGap(_gaps[i],i+1);
i++;
//cout<<" Character "<<i<<" "<< character_p->getCoord5()<<" "<< character_p->getCoord3()<<" " <<endl;
//break;
}
}
LOGnOUT(4,<<endl<< "There were "<<_characters.size()<<" characters"<<endl);
}
/********************************************************************************************
delimitationOfCharactersMCIC2
*********************************************************************************************/
//void indelCoder::delimitationOfCharactersMCIC2(){
// LOGnOUT(4,<<endl<< "Step (2) delimitationOfCharacters... Complex Coding (MCIC2)"<<endl);
// LOGnOUT(5,<< " Finding the required positions(=characters) in the coded sequence"<<endl);
// LOGnOUT(5,<< " The number of characters <= the number of found gaps (each character may include several gaps)"<<endl);
//
// int i=0;
// character* character_p = new character(_gaps[i]->getCoord5(), _gaps[i]->getCoord3(),_sc.numberOfSeqs());
// _characters.push_back(character_p);
// while( i<_gaps.numOfGaps())
// {
// //coord5_c = _gaps[i]->getCoord5();
// //coord3_c = _gaps[i]->getCoord3();
// while( _gaps[i]->getCoord5()>=character_p->getCoord5() && _gaps[i]->getCoord3()<=character_p->getCoord3()){
// character_p->addGap(_gaps[i],i+1);
// i++;
// if(i>=_gaps.numOfGaps())
// break;
// }
// // gap_i is extending previous character it's start is included in the previous character
// while(i<_gaps.numOfGaps()
// && _gaps[i]->getCoord5()<=character_p->getCoord3() && _gaps[i]->getCoord3()>character_p->getCoord3() ){
// character_p->setCoord3(_gaps[i]->getCoord3());
// character_p->addGap(_gaps[i],i+1);
// i++;
// if(i>=_gaps.numOfGaps())
// break;
// }
// // new character is required for this gap
// if(i<_gaps.numOfGaps() && _gaps[i]->getCoord5() > character_p->getCoord5() && _gaps[i]->getCoord3() > character_p->getCoord3()){
// character_p = new character(_gaps[i]->getCoord5(), _gaps[i]->getCoord3(),_sc.numberOfSeqs());
// _characters.push_back(character_p);
// character_p->addGap(_gaps[i],i+1);
// i++;
// //cout<<" Character "<<i<<" "<< character_p->getCoord5()<<" "<< character_p->getCoord3()<<" " <<endl;
// //break;
// }
// }
// LOGnOUT(4,<<endl<< "There were "<<_characters.size()<<" characters"<<endl);
//}
/********************************************************************************************
delimitationOfCharactersSIC
*********************************************************************************************/
void indelCoder::delimitationOfCharactersSIC(){
LOGnOUT(4,<<endl<< "Step (2) delimitationOfCharacters... Simple Coding (SIC)"<<endl);
LOGnOUT(5,<< " Finding the required positions(=characters) in the coded sequence"<<endl);
LOGnOUT(5,<< " The number of characters <= the number of found gaps (each character may include several gaps)"<<endl);
int i=0;
character* character_p=NULL;
if (_gaps.numOfGaps()>0) {
character_p = new character(_gaps[i]->getCoord5(), _gaps[i]->getCoord3(),_sc.numberOfSeqs());
_characters.push_back(character_p);
}
while( i<_gaps.numOfGaps())
{
while( _gaps[i]->getCoord5()==character_p->getCoord5() && _gaps[i]->getCoord3()==character_p->getCoord3()){
character_p->addGap(_gaps[i],i+1);
i++;
if(i>=_gaps.numOfGaps())
break;
}
// new character is required for this gap
if(i<_gaps.numOfGaps() ){
character_p = new character(_gaps[i]->getCoord5(), _gaps[i]->getCoord3(),_sc.numberOfSeqs());
_characters.push_back(character_p);
character_p->addGap(_gaps[i],i+1);
i++;
//cout<<" Character "<<i<<" "<< character_p->getCoord5()<<" "<< character_p->getCoord3()<<" " <<endl;
//break;
}
}
LOGnOUT(4,<<endl<< "There were "<<_characters.size()<<" characters"<<endl);
}
/********************************************************************************************
determinationCharacterState
// 2) determination of the character state of each character.
// Each sequence presenting a different indel pattern at the corresponding character region is coded as a different state.
// state_0 is defined by no-gaps in this region
// Foreach j in character_1:character_M (M where found in previous step)
// Foreach s in seq1:seqS (S taxons in the MSA)
// if s gaps coordinates are equal to one of the gaps coordinates of previous states
// next;
// else
// st++, where state_st is defined by X set of 5'-3' coordinates of the X gaps within s
*********************************************************************************************/
void indelCoder::determinationCharacterState(){
LOGnOUT(4,<<endl<< "Step (3) determinationCharacterState... "<<endl);
// loop over characters
for(int c = 0; c < _characters.size(); ++c)
{
int coord_5p = _characters[c]->getCoord5();
int coord_3p = _characters[c]->getCoord3();
_characters[c]->addZeroState(); // the default state - ones in length of the character
//cout<<" Char "<<" "<<coord_5p<<" "<< coord_3p<<endl;
_characters[c]->resizeSc_states(); // the _sc_states matrix (#species X length) is init with ones
// loop over taxa
for(int s = 0; s < _sc.numberOfSeqs(); ++s){
int seqID = _sc[s].id();
if(seqID != s)
cout<<"error: seqID not eq s";
//cout<<"SeqID vs. s "<<seqID<<" "<<s<<endl; // DEBUG
// loop over gaps - ToDo - highly wasteful! - fix
for(int g = 0; g <_gapsVperSc[seqID].numOfGaps(); ++g){
int coord_5_gap = _gapsVperSc[seqID][g]->getCoord5();
int coord_3_gap = _gapsVperSc[seqID][g]->getCoord3();
if(coord_5_gap>=coord_5p && coord_3_gap<= coord_3p){
// if the gap of the species is included in character - update _sc_state with zeros to designate this gap
_characters[c]->setGapsInSc_states( seqID, coord_5_gap, coord_3_gap);
}
}
bool isNewState = true;
if(_characters[c]->getScStates()[s]==_characters[c]->getStates()[0] ){
isNewState = false;
_matrix[s][c] = 0; // no gaps for species s in character c
}
else{
for(int sq = s-1; sq>=0; --sq){
if(_characters[c]->getScStates()[s] == _characters[c]->getScStates()[sq] ){
isNewState = false; // this state was already found, no need for new
_matrix[s][c] = _matrix[sq][c]; // gap in species s, same state as previously found state in species sq
}
}
}
if(isNewState){ // state was not found in previous species, need new
_characters[c]->addState(_characters[c]->getScStates()[s]);
_matrix[s][c] = _characters[c]->getNumOfStates()-1; // gap in species s, new state type
}
}
}
}
/********************************************************************************************
determinationCharacterState
// 2) determination of the character state of each character.
// Each sequence presenting a different indel pattern at the corresponding character region is coded as a different state.
// state_0 is defined by no-gaps in this region
// state_1 for species with this (exact) gap
// state_? for species with gap overlapping this one
*********************************************************************************************/
void indelCoder::determinationCharacterStateSIC(){
LOGnOUT(4,<<endl<< "Step (3) determinationCharacterState... "<<endl);
resizeMatrix(_matrix,_sc.numberOfSeqs(),_characters.size()); // all zeroes
// loop over characters
for(int c = 0; c < _characters.size(); ++c)
{
int coord_5_char = _characters[c]->getCoord5();
int coord_3_char = _characters[c]->getCoord3();
// loop over all gaps
for(int g = 0; g <_gaps.numOfGaps(); ++g){
int coord_5_gap = _gaps[g]->getCoord5();
int coord_3_gap = _gaps[g]->getCoord3();
int s = _gaps[g]->getSeqID(); // ????
string nameG = _sc.name(_gaps[g]->getSeqID());
if(coord_5_gap==coord_5_char && coord_3_gap==coord_3_char){
_matrix[s][c] = 1;
}
else if( //(coord_5_gap>=coord_5_char && coord_3_gap<= coord_3_char) || // gap (in genome 'g') is within the char (indel=c)
(coord_5_gap<=coord_5_char && coord_3_gap>= coord_3_char ) //|| // char (indel=c) is within the gap (in genome 'g')
//(coord_5_gap<=coord_5_char && coord_3_gap>=coord_5_char ) || // 5' of char is within gap (partial overlap)
//(coord_5_gap<=coord_3_char && coord_3_gap>=coord_3_char ) // 3' of char is within gap (partial overlap)
)
_matrix[s][c] = 2; // same as '?', need to find&replace
}
for(int g = 0; g <_unknowns.numOfGaps(); ++g){
int coord_5_gap = _unknowns[g]->getCoord5();
int coord_3_gap = _unknowns[g]->getCoord3();
int s = _unknowns[g]->getSeqID(); // ????
if(coord_5_gap<=coord_5_char && coord_3_gap>= coord_3_char )
_matrix[s][c] = 2; // same as '?', need to find&replace
if(_matrix[s][c] == 1 && (coord_5_char==(coord_3_gap+1) || coord_3_char==(coord_5_gap-1)) ) // the indel is flaked by unKnown, thus it is ?
_matrix[s][c] = 2; // same as '?', need to find&replace
}
}
}
/********************************************************************************************
determinationStepsMatrix
// 3) determination of the number of steps between every 2 character states.
// Each pair of sequences is compared separately for the corresponding character area and the minimum number of steps between every 2 character states is then determined
// cost_c_x_y is initiated with lenght of character c
// Foreach c in character_1:character_M
// Foreach st_x and st_y in state_0:state_c_ST (There are ST states in character c) (go over all state combinations)
// Do A to E steps for the pair st_x and st_y:
// A) translate into 01 to X set of 5'-3' coordinats of the X gaps within st_x and st_y
// B) ignore 0-0 (cost_c_x_y =- #0-0 colomns)
// C) merge adjacent 0-1 and 1-0 ((cost_c_x_y =- #adjacent 0-1 and 1-0 colomns)
// D) ignore 1-1 (cost_c_x_y =- #1-1 colomns)
*********************************************************************************************/
void indelCoder::determinationStepsMatrix(){
LOGnOUT(4,<<endl<< "determinationStepsMatrix..."<<endl);
for(int c = 0; c < _characters.size(); ++c)
{
_characters[c]->determinationStepsMatrix();
}
}
/********************************************************************************************
// print to out file the required data
*********************************************************************************************/
void indelCoder::printGapsInfo(){
string fileGapsString = "gapsInfo.txt";
ofstream fileGapsStream(fileGapsString.c_str());
fileGapsStream<<"# Start coordinate are with the genome as reference (not MSA)."<<endl;
fileGapsStream<<"# Count starts from zero (first position = 1)."<<endl;
fileGapsStream<<"seqID"<<"\t"<<"seqName"<<"\t"<<"start"<<"\t"<<"length"<<endl;
int gapNum = 1;
for(int s = 0; s < _sc.numberOfSeqs(); ++s){
int seqID = _sc[s].id();
string seqName = _sc[s].name();
for(int g = 0; g <_gapsVperSc[seqID].numOfGaps(); ++g){
fileGapsStream<<seqID<<"\t"<<seqName<<"\t"<<_gapsVperSc[seqID][g]->getCoord5Abs()+1<<"\t"<<_gapsVperSc[seqID][g]->getLength()<<endl;
}
}
}
/********************************************************************************************
// print to out file the required data as fasta file
*********************************************************************************************/
void indelCoder::printFasta(){
//string fileString = indelCoderOptions::_outDir + "//" + "outFileCodedSeq.fa";
//ofstream fileStream(fileString.c_str());
ofstream fileStream(indelCoderOptions::_indelOutputFastaFile.c_str());
bool isSIC = indelCoderOptions::_codingType == indelCoderOptions::SIC;
for(int s=0; s<_sc.numberOfSeqs();++s){
fileStream<<">"<<_sc.name(s)<<"\n";
for(int c=0; c<_matrix[0].size(); ++c ){
if(isSIC && _matrix[s][c]==2)
fileStream<<'?';
else
fileStream<<_matrix[s][c];
}
fileStream<<endl; // prev- 2 endl
}
fileStream.close();
}
/********************************************************************************************
// 4) print to out file the required data
// 4.1) MATRIX of S species over M characters
// 4.2) foreach character of more than 2 states print the transition costs stepmatrix
*********************************************************************************************/
void indelCoder::printNexus() {
bool isSIC = indelCoderOptions::_codingType == indelCoderOptions::SIC;
string fileNexusString = indelCoderOptions::_nexusFileName;
ofstream fileNexusStream(fileNexusString.c_str());
fileNexusStream<<"#NEXUS"<<endl<<endl;
fileNexusStream<<"[!matrix with indels coded according to "<<indelCoderOptions::getCodingType(indelCoderOptions::_codingType)<<" coding]"<<endl;
//if(indelCoderOptions::_isMCIC2){fileNexusStream<<"2";}
//fileNexusStream<<"]"<<endl;
fileNexusStream<<"[! "<< PROG_INFO <<" ]"<<endl<<endl;
fileNexusStream<<"BEGIN CHARACTERS;"<<endl;
fileNexusStream<<"DIMENSIONS newtaxa ntax="<<_sc.numberOfSeqs()<<" NCHAR="<<_sc.seqLen()+_characters.size()<<";"<<endl<<endl;
fileNexusStream<<"FORMAT "<<endl;
fileNexusStream<<" DATATYPE = standard"<<endl;
fileNexusStream<<" GAP = - "<<endl;
fileNexusStream<<" MISSING = ?"<<endl;
fileNexusStream<<" SYMBOLS="<<'"'<<"0123456789A#C$EFG.IJ&L%>OPQ/'TU:*X<Z"<<'"'<<endl;
fileNexusStream<<" EQUATE="<<'"'<<"R={AG} Y={CT} M={AC} K={GT} S={CG} W={AT} H={ACT} B={CGT} V={ACG} D={AGT} N={ACGT} r={AG} y={CT} m={AC} k={GT} s={CG} w={AT} h={ACT} b={CGT} v={ACG} d={AGT} n={ACGT}"<<'"'<<endl;
fileNexusStream<<"INTERLEAVE;"<<endl;
//string fileGapString = indelCoderOptions::_outDir + "//" + "outGapInfoCHARSTATELABELS.txt";
//ofstream gapStream(fileGapString.c_str());
//gapStream<<"# each character is listing all included gaps.\n";
//gapStream<<"# date for each gap: seqID(num start from 0), coord5Abs(not by MSA), length.\n";
fileNexusStream<<"CHARSTATELABELS"<<endl;
int seqLeng = _sc.seqLen();
int c=0;
for(c=0; c<_characters.size();++c){
fileNexusStream<<"\t"<<c+1+seqLeng<<" "<<"ind_pos_"<<_characters[c]->getCoord5()+1<<"_to_"<<_characters[c]->getCoord3()+1<<" ";
fileNexusStream<<"/absent ";
// gapStream<<c<<" character\t"<<_characters[c]->getCoord5()+1<<" to "<<_characters[c]->getCoord3()+1<<"\tincluding gaps:";
for(int g=0; g<_characters[c]->getGapsIndices().size(); ++g){
int gapNum = _characters[c]->getGapsIndices()[g];
fileNexusStream<<" indel_"<<gapNum;
//gapStream<<"\tgap "<<gapNum<<": "<<_gaps[gapNum-1]->getSeqID();
//gapStream<<", "<<_gaps[gapNum-1]->getCoord5Abs()<<", "<<_gaps[gapNum-1]->getLength()<<";";
}
fileNexusStream<<endl;
//gapStream<<endl;
}
fileNexusStream<<"MATRIX"<<endl<<endl;
for(int s=0; s<_sc.numberOfSeqs();++s){
fileNexusStream<<""<<_sc.name(s)<<""; //prev- "Species_"
for(c=0; c<_matrix[0].size(); ++c ){
if(isSIC && _matrix[s][c]==2)
fileNexusStream<<'?';
else
fileNexusStream<<_matrix[s][c];
}
fileNexusStream<<endl; // prev- 2 endl
}
fileNexusStream<<";"<<endl<<endl;
fileNexusStream<<"END;"<<endl<<endl<<endl;
fileNexusStream<<"BEGIN ASSUMPTIONS; [below are the cost matrices of character change between the indel character state, these matrix exist only for characters that have more than two states]"
<<endl<<endl<<endl<<endl;
if(!isSIC){
for(c=0; c<_characters.size();++c){
int numOfStates = _characters[c]->getNumOfStates();
if(numOfStates>2){
fileNexusStream<<"[char "<<c+1+seqLeng<<", indel char "<<c+1<<" "<<"("<<_characters[c]->getCoord5()+1<<"-"<<_characters[c]->getCoord3()+1<<"):";
fileNexusStream<<"0 (absent)";
for(int st=1; st<numOfStates; ++st ){
fileNexusStream<<", "<<st<<" (indel_"<<_characters[c]->getGapsIndices()[st] <<")";
}
fileNexusStream<<" ]"<<endl;
fileNexusStream<<"usertype stepmatrix"<<c+1+seqLeng<<" (stepmatrix)="<<numOfStates<<endl<<endl;
if(indelCoderOptions::_isCheckForTriangleInequality){
if(_characters[c]->isTriangleInequalityCorrectionNeeded()){
_characters[c]->printStepsMatrix(fileNexusStream,_characters[c]->isTriangleInequalityCorrectionNeeded());
fileNexusStream<<"\n[prior to adjustment to satisfy triangle inequality:]\n";
fileNexusStream<<"[";
_characters[c]->printStepsMatrix(fileNexusStream);
fileNexusStream<<"]\n";
}
else{
_characters[c]->printStepsMatrix(fileNexusStream);
}
}
else{
_characters[c]->printStepsMatrix(fileNexusStream);
}
fileNexusStream<<";"<<endl<<endl<<endl;
}
}
fileNexusStream<<endl<<endl<<endl;
fileNexusStream<<"[below is the line that says to which indel correspond which matrix]"<<endl<<endl;
fileNexusStream<<"typeset complexIndelCoding = ";
for(c=0; c<_characters.size()-1;++c){
int numOfStates = _characters[c]->getNumOfStates();
if(numOfStates>2){
fileNexusStream<<"stepmatrix"<<c<<" :"<<c<<",";
}
}
fileNexusStream<<"stepmatrix"<<c<<" :"<<c<<";"<<endl<<endl;
fileNexusStream<<";"<<endl<<endl;
fileNexusStream<<"END;"<<endl<<endl<<endl<<endl;
}
fileNexusStream<<"BEGIN SETS;"<<endl<<endl;
fileNexusStream<<"CHARSET indels = 1-"<<_characters.size()<<";"<<endl<<endl;
fileNexusStream<<"END;"<<endl<<endl<<endl;
fileNexusStream<<"BEGIN PAUP;"<<endl<<endl;
fileNexusStream<<"assume typeset=complexIndelCoding;"<<endl<<endl;
fileNexusStream<<"END;"<<endl<<endl;
fileNexusStream<<"[Indels:"<<endl;
fileNexusStream<<"No. extension"<<endl;
//for(int c=0; c<_gaps.numOfGaps();++c){
// fileNexusStream<<c+1<<"\t"<<_gaps[c]->getCoord5()<<"-"<<_gaps[c]->getCoord3()<<endl;
//}
for(c=0; c<_characters.size();++c){
fileNexusStream<<c+1<<"\t"<<_characters[c]->getCoord5()+1<<"-"<<_characters[c]->getCoord3()+1<<endl;
}
fileNexusStream<<"]"<<endl;
}
void indelCoder::printIndelSummary() {
bool isSIC = indelCoderOptions::_codingType == indelCoderOptions::SIC;
//string fileGapString = indelCoderOptions::_outDir + "//" + "outGapInfoCHARSTATELABELS.txt";
//ofstream gapStream(fileGapString.c_str());
ofstream gapStream(indelCoderOptions::_indelOutputInfoFile.c_str());
gapStream<<"# each character is listing all included gaps.\n";
gapStream<<"# Character: Start position relative to MSA (first pos of gap, count from 0); End position relative to MSA (+1); length.\n";
gapStream<<"# Gap: seqID(num start from 0); coord5Abs (relative to genome. not by MSA,first pos of gap, count from 0); length.\n";
int seqLeng = _sc.seqLen();
for(int c=0; c<_characters.size();++c){
gapStream<<"character number: "<<c<<endl;
gapStream<<"Start position relative to MSA: "<<_characters[c]->getCoord5()<<endl;
gapStream<<"End position relative to MSA: "<<_characters[c]->getCoord3()+1<<endl;
gapStream<<"Length: "<<_characters[c]->getCoord3()-_characters[c]->getCoord5()+1<<endl;
vector<bool> isSpeciesWithGap(_sc.numberOfSeqs(),false);
for(int g=0; g<_characters[c]->getGapsIndices().size(); ++g){
int gapNum = _characters[c]->getGapsIndices()[g];
int speciesSeqID = _gaps[gapNum-1]->getSeqID();
isSpeciesWithGap[speciesSeqID] = true;
gapStream<<"Found in species: "<<_sc.name(speciesSeqID);
gapStream<<" Start position relative to genome: "<<_gaps[gapNum-1]->getCoord5Abs();
gapStream<<" Length: "<<_gaps[gapNum-1]->getLength()<<endl;
//gapStream<<"\tgap "<<gapNum<<": "<<_gaps[gapNum-1]->getSeqID();
//gapStream<<", "<<_gaps[gapNum-1]->getCoord5Abs()<<", "<<_gaps[gapNum-1]->getLength()<<";";
}
gapStream<<"NOT FOUND in species: ";
for(int i=0; i<_sc.numberOfSeqs(); ++i){
if(!isSpeciesWithGap[i] && _matrix[i][c]!=2)
gapStream<<_sc.name(i)<<",";
}
gapStream<<"\n";
gapStream<<"ENDCHARACTER"<<endl<<endl;
}
}
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