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package Bio::Graphics::Browser2::DataLoader::archive;
# $Id$
use strict;
use base 'Bio::Graphics::Browser2::DataLoader';
use Bio::DB::BigWigSet;
use File::Spec;
sub new {
my $class = shift;
my $self = $class->SUPER::new(@_);
$self->{default_track_name} = 'track000';
$self;
}
sub default_track_name {
my $self = shift;
return $self->{default_track_name}++;
}
sub load {
my $self = shift;
my ($initial_lines,$fh) = @_;
$self->flag_busy(1);
eval {
$self->open_conf;
$self->set_status('starting load');
mkdir $self->sources_path or die $!;
$self->{archive} = File::Spec->catfile($self->sources_path,$self->track_name);
my $source_file = IO::File->new($self->{archive},'>');
warn "sourcefile=$self->{archive}";
$self->start_load;
$self->set_status('load data');
my $bytes_loaded = 0;
foreach (@$initial_lines) {
$source_file->print($_);
$bytes_loaded += length $_;
}
my $buffer;
while ((my $bytes = read($fh,$buffer,8192) > 0)) {
$source_file->print($buffer);
$bytes_loaded += length $ buffer;
$self->set_status("loaded $bytes_loaded bytes") if $bytes++ % 10000;
}
$source_file->close();
$self->finish_load;
$self->check_metadata;
$self->write_conf;
$self->close_conf;
$self->set_processing_complete;
};
$self->flag_busy(0);
die $@ if $@;
return $self->tracks;
}
sub finish_load {
my $self = shift;
$self->set_status("extracting files from archive");
my $archive = $self->{archive};
my $bin = $archive =~ /\.tar$/i ? $self->search_for_binary('tar') :
$archive =~ /\.zip$/i ? $self->search_for_binary('unzip') :
'';
die "unrecognized file type!" unless $bin;
my $command = $archive =~ /\.tar$/i ? "$bin -tf" : "$bin -l";
my $fh;
open $fh, "($command $archive && echo 'success') 2>&1 |";
my @contents = <$fh>;
close $fh;
unless ($contents[-1] =~ /success/) {
die "ARCHIVE LIST ERROR: @contents";
}
pop @contents;
my @to_extract;
while (@contents) {
my $item = shift @contents;
next if ($item =~ /^(Archive\:| Length|\-{5,})/); # unzip headers
$item = (split / {2,}/, $item)[-1]; # split on two or more spaces
chomp $item;
my (undef, undef, $file) = File::Spec->splitpath($item);
if ($file =~ /^meta/i) {
push @to_extract, [$item, $file];
}
elsif ($file =~ /^\./) {
next; # skip .files if present
}
elsif ($file =~ /\.bw$/i) {
push @to_extract, [$item, $file];
}
}
die "no recognizable files in archive to extract" unless @to_extract;
# we are trying to be safe here and extract just the file contents
# without directory paths to avoid ill- or mis-intentioned stray files
my $path = $self->sources_path;
my $count;
$command = $archive =~ /\.tar$/i ? "$bin -x -O -f" : "$bin -p";
foreach (@to_extract) {
my $target = File::Spec->catfile($path, $_->[1]);
# extract file from tar archive and redirect to file in our path
open my $in, "$command $archive \"$_->[0]\" |" or
die "unable to open achive for extraction";
open my $out, '>', $target or die "unable to open target file";
while (<$in>) {print $out $_}
close $in;
close $out;
$count++ if -e $target && -s _ ;
}
die "not all files could be extracted\n" unless $count == scalar(@to_extract);
unlink $archive; # no longer need
}
sub search_for_binary {
my $self = shift;
my $target = shift;
for my $p (split ':', $ENV{PATH}) {
my $tgt = File::Spec->catfile($p,$target);
return $tgt if -e $tgt && -x _;
}
return;
}
sub check_metadata {
my $self = shift;
$self->set_status('checking metadata');
my $path = $self->sources_path;
my $count = Bio::DB::BigWigSet->index_dir($path);
die "two or more BigWig files are required" unless $count > 1;
my $bws = Bio::DB::BigWigSet->new(-dir => $path);
my $md = $bws->metadata;
my $default = $self->track_name; # default type
my %types; # metadata types, not required to be unique
my %names; # metadata display_names, required to be unique
my $flag; # for updated metadata
foreach my $i (keys %$md) {
if (exists $md->{$i}{'type'}) {
$types{ $md->{$i}{'type'} }++;
}
elsif (exists $md->{$i}{'method'} && exists $md->{$i}{'source'}) {
$types{ $md->{$i}{'method'} . ':' . $md->{$i}{'source'} }++;
}
elsif (exists $md->{$i}{'method'}) {
$types{ $md->{$i}{'method'} }++;
}
elsif (exists $md->{$i}{'primary_tag'}) {
$types{ $md->{$i}{'primary_tag'} }++;
}
else {
$types{$default}++;
my (undef, undef, $filename) = File::Spec->splitpath( $md->{$i}{'dbid'} );
$bws->set_bigwig_attributes($md->{$i}{'dbid'}, {'type'=>$default});
$flag++;
}
if (exists $md->{$i}{'display_name'}) {
if (exists $names{ $md->{$i}{'display_name'} }) {
# must make the name unique
my (undef, undef, $name) = File::Spec->splitpath( $md->{$i}{'dbid'} );
$name =~ s/\.bw$//i;
$bws->set_bigwig_attributes(
$md->{$i}{'dbid'}, {'display_name'=>$name} );
$names{$name}++;
$flag++;
}
else {
$names{ $md->{$i}{'display_name'} }++;
}
}
else {
my (undef, undef, $name) = File::Spec->splitpath( $md->{$i}{'dbid'} );
$name =~ s/\.bw$//i;
$bws->set_bigwig_attributes(
$md->{$i}{'dbid'}, {'display_name'=>$name} );
$names{$name}++;
$flag++;
}
}
$self->write_new_metadata($bws) if $flag;
$self->typelist(keys %types);
$self->namelist(keys %names);
}
sub typelist {
my $self = shift;
$self->{typelist} ||= [];
@{ $self->{typelist} } = @_ if @_;
return @{ $self->{typelist} };
}
sub namelist {
my $self = shift;
$self->{namelist} ||= [];
@{ $self->{namelist} } = @_ if @_;
return @{ $self->{namelist} };
}
sub write_new_metadata {
# Bio::DB::BigWigSet does not have a method to write out updated metadata
# so we will do it here
my $self = shift;
my $bws = shift;
my $path = $self->sources_path;
foreach my $f (glob($path)) {
unlink $f if $f =~ /^meta/i;
}
my $index = File::Spec->catfile($path, 'metadata.index');
open my $fh, ">", $index or return;
my $md = $bws->metadata;
foreach my $i (sort {$a <=> $b} keys %$md) {
my (undef, undef, $file) = File::Spec->splitpath( $md->{$i}{'dbid'} );
my $string = "[$file]\n";
foreach my $k (keys %{$md->{$i}}) {
next if $k eq 'dbid';
$string .= "$k = " . $md->{$i}{$k} . "\n";
}
print $fh "$string\n";
}
close $fh;
}
sub write_conf {
my $self = shift;
my $name = $self->track_name;
my $path = $self->sources_path;
my $loadid = $self->loadid;
my $conf = $self->conf_fh;
my $dbid = $self->new_track_label;
my @types = $self->typelist;
my $table;
foreach my $n ($self->namelist) {
$table .= "\n :\"$n\" \"$n\" ;";
}
print $conf <<END;
[$dbid:database]
db_adaptor = Bio::DB::BigWigSet
db_args = -dir '$path'
-feature_type summary
#>>>>>>>>>> cut here <<<<<<<<
[$dbid]
database = $dbid
feature = @types
subtrack select = Name tag_value display_name
subtrack table = $table
glyph = wiggle_whiskers
# change glyph to wiggle_xyplot to use semi-transparent overlap
fgcolor = black
height = 50
autoscale = chromosome
key = $name
description =
END
;
}
1;
__END__
=head1 NAME
Bio::Graphics::Browser2::DataLoader::archive
=head1 DESCRIPTION
A data loader to work with archives of BigWig files to generate a BigWigSet
database. Two or more BigWig files may be combined into an archive file and
uploaded. The files are extracted, a metadata index generated if required, and
a configuration written using subtracks for each BigWig file. This allows a
fast, convenient option to bundle multiple data files together and provide a
concise, organized interface to multiple data tracks.
Supported archives include TAR (.tar, .tar.gz, .tgz, .tbz2, .tar.bz2) and
ZIP (.zip) files. Archives should include only BigWig files (which must have
a .bw extension) and optionally a metadata text file. Extraneous files and
directory paths are ignored.
Subtrack tables are set up using the display_name tag value.
See the documentation for Bio::DB::BigWigSet for more information.
=head1 SETUP
The Bio-BigFile (Bio::DB::BigWigSet) Perl module must be installed.
Archives are processed through external tar and unzip utilities. These
are located by searching the default environment PATH.
=head1 AUTHOR
Timothy J. Parnell, PhD
Dept of Oncological Sciences
Huntsman Cancer Institute
University of Utah
Salt Lake City, UT, 84112
This package is free software; you can redistribute it and/or modify
it under the terms of the GPL (either version 1, or at your option,
any later version) or the Artistic License 2.0.
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