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package Bio::Graphics::Browser2::UserTracks;
# $Id$
use strict;
use Bio::Graphics::Browser2::DataSource;
use Bio::Graphics::Browser2::DataLoader;
use Bio::Graphics::Browser2::UserTracks::Filesystem;
use Bio::Graphics::Browser2::UserTracks::Database;
use File::Spec;
use File::Basename 'basename';
use File::Path 'mkpath','rmtree';
use File::Copy 'move';
use IO::File;
use IO::String;
use File::Temp 'tempdir';
use POSIX ();
use Carp qw(croak cluck);
use CGI 'param';
use Data::Dumper;
use constant DEBUG => 0;
my $HASBIGWIG;
# The intent of this is to provide a single unified interface for
# managing a user's uploaded and shared tracks.
# BUG: It's still kind of screwy: there seem to be filesystem
# traversal methods in the parent class which should not be here.
# class methods
sub busy_file_name { 'BUSY' }
sub status_file_name { 'STATUS' }
sub imported_file_name { 'IMPORTED' }
sub mirrored_file_name { 'MIRRORED' }
sub sources_dir_name { 'SOURCES' }
sub new {
my $class = shift;
my ($data_source,$session) = @_;
my $globals = $data_source->globals;
my $uploadsid = $session->uploadsid;
my $backend = $globals->user_account_db;
my $user_accounts = $globals->user_accounts;
my $implementer;
if ($backend && $backend =~ /db/i && $user_accounts) {
$implementer = 'Bio::Graphics::Browser2::UserTracks::Database';
} else {
$implementer = 'Bio::Graphics::Browser2::UserTracks::Filesystem';
}
my $self = $implementer->_new($data_source, $globals, $uploadsid, $session);
$self->is_admin(1) if $class->admin;
$self->create_track_lookup;
return $self;
}
sub _new {
my $class = shift;
my ($data_source, $globals, $uploadsid, $session) = @_;
my $self = bless {
config => $data_source,
uploadsid => $uploadsid,
globals => $globals,
session => $session,
data_source => $data_source->name,
}, ref $class || $class;
return $self;
}
##### CLASS METHOD #####
sub admin {0}
########################
sub uploadsid {shift->{uploadsid}}
sub session {shift->{session}}
sub sessionid {shift->session->id}
sub page_settings {
my $self = shift;
return $self->{'.page_settings'} ||= $self->session->page_settings;
}
sub database {
my $self = shift;
return $self->isa('Bio::Graphics::Browser2::UserTracks::Database');
}
sub globals {shift->{globals} }
sub data_source {shift->{config} }
sub datasource_name {shift->{data_source} }
sub is_admin {
my $self = shift;
my $d = $self->{is_admin};
$self->{is_admin} = shift if @_;
$d;
}
# Tracks - Returns an array of paths to a user's tracks.
sub tracks {
my $self = shift;
my @tracks;
push @tracks, $self->get_uploaded_files, $self->get_imported_files;
push @tracks, $self->get_added_public_files, $self->get_shared_files;
return @tracks;
}
# Track Lookup - Returns a hash of tracks showing the files they originate from.
sub track_lookup {
my $self = shift;
return %{$self->{track_lookup}};
}
# Create Track Lookup - Populate the track lookup hash.
sub create_track_lookup {
my $self = shift;
my @tracks = $self->tracks;
my $track_lookup = {};
foreach my $track (@tracks) {
$track_lookup->{$_} = $track foreach $self->labels($track);
}
$self->{track_lookup} = $track_lookup;
}
# Get Track Upload ID - Returns the upload ID of the original file the track came from.
sub get_track_upload_id {
my $self = shift;
my $track = shift;
my %track_lookup = $self->track_lookup;
return $track_lookup{$track};
}
# Is Mirrored - Returns the URL if a specific file is mirrored.
sub is_mirrored {
my $self = shift;
my $file = shift;
my $filename = $self->filename($file);
my $mirror_flag = $self->mirror_flag($file);
return unless -e $mirror_flag;
open(my $i, $mirror_flag);
my $url = <$i>;
close $i;
return $url;
}
# Set Mirrored - Sets the mirror flag.
sub set_mirrored {
my $self = shift;
my $file = shift;
my $filename = $self->filename($file);
my $url = shift;
my $flagfile = $self->mirror_flag($file);
open my $i,">", $flagfile or warn "can't open mirror file: $!";
print $i $url;
close $i;
}
# Conf Files (File) - Returns the paths to all tracks' conf files.
sub conf_files {
my $self = shift;
my $file = shift;
return grep {-e $_} map { $self->track_conf($file) } $self->tracks;
}
sub path {
my $self = shift;
my $uploadsid = $self->uploadsid;
my $source = $self->data_source;
my $path = $self->is_admin ? $source->admindata() : $source->userdata($uploadsid);
return $path;
}
# Track Path (File) - Returns a verified path to the folder holding a track.
sub track_path {
my $self = shift;
my $track = shift;
my $filename = $self->filename($track);
if ($filename =~ m!/!) {
my ($uploadsid,$base) = split '/',$filename;
return File::Spec->catfile($self->data_source->userdata($uploadsid),$self->escape_url($base));
} else {
my $folder_name = $self->escape_url($filename);
return File::Spec->catfile($self->path($track), $folder_name);
}
}
# Blind Track Path (Filename) - Blindly attaches the userdata path to whatever filename you give it.
sub blind_track_path {
my $self = shift;
my $filename = shift;
return File::Spec->catfile($self->path, $filename);
}
# Data Path (File, Data File) - Returns the full path to a track's original data file.
sub data_path {
my $self = shift;
my $file = shift;
my $filename = $self->filename($file);
my $datafile = shift;
return File::Spec->catfile($self->track_path($file), $self->sources_dir_name, $datafile);
}
# Track Conf (File) - Returns the full path to the track's configuration file.
sub track_conf {
my $self = shift;
my $file = shift;
my $filename = $self->filename($file);
my $conf_file = $self->escape_url($filename) . ".conf";
return File::Spec->catfile($self->track_path($file), $conf_file);
}
# Conf FH (File) - Returns a file handle to a track's configuration file.
sub conf_fh {
my $self = shift;
my $file = shift;
my $filename = $self->filename($file);
return Bio::Graphics::Browser2::UserConf->fh($self->track_conf($file));
}
# Import Flag (File) - Returns a track's import flag file.
sub import_flag {
my $self = shift;
my $file = shift;
my $filename = $self->filename($file);
return File::Spec->catfile($self->track_path($file), $self->imported_file_name);
}
# Mirror Flag (File) - Returns the location of the mirror flag file.
sub mirror_flag {
my $self = shift;
my $file = shift;
my $filename = $self->filename($file);
return File::Spec->catfile($self->track_path($file), $self->mirrored_file_name);
}
# Conf Metadata (File) - Returns the modified time and size of a track's configuration file.
sub conf_metadata {
my $self = shift;
my $file = shift;
my $filename = $self->filename($file);
my $conf = $self->track_conf($file);
my $name = basename($conf);
return ($name, (stat($conf))[9, 7]);
}
# Source Files (File) - Returns an array of source files (with details) associated with a specified track.
sub source_files {
my $self = shift;
my $file = shift;
my $filename = $self->filename($file);
my $path = File::Spec->catfile($self->track_path($file), $self->sources_dir_name);
my @files;
if (opendir my $dir, $path) {
while (my $f = readdir($dir)) {
my $path = File::Spec->catfile($path, $f);
next unless -f $path;
my ($size, $mtime) = (stat(_))[7,9];
push @files, [$f, $size, $mtime, $path];
}
}
return @files;
}
# Escape URL (URL[, Uniquefy]) - Gets an escaped name from a given URL.
sub escape_url {
my $self = shift;
my $url = shift;
my $uniquefy = shift;
my $filename;
# Remove any illegal chars
# hack here: if the URL looks like a "uploadid/filename", then we
# leave the slash there.
if ($url =~ m!^[a-f0-9]{32}/!) { # treat as a path to another userid
my ($dir,$rest) = split '/',$url;
$rest =~ tr/a-zA-Z0-9_%^@.-/_/cs;
$filename = $rest;
} else {
($filename = $url) =~ tr/a-zA-Z0-9_%^@.-/_/cs;
}
# Cut the length at the maximum filename.
if (length $filename > $self->max_filename) {
$filename = substr($filename, 0, $self->max_filename);
}
# If the file isn't unique, add a number on the end.
my $unique = 0;
while ($uniquefy && !$unique) {
my $path = $self->blind_track_path($filename);
if (-e $path) {
$filename .= "-0" unless $filename =~ /-\d+$/;
$filename =~ s/-(\d+)$/'-'.($1+1)/e; # add +1 to the trackname
} else {
$unique++;
}
}
return $filename;
}
# Trackname from URL (URL[, Uniquefy]) - Gets a track name from a given URL and creates the folder to hold it.
sub trackname_from_url {
my $self = shift;
my $url = shift;
my $uniquefy = shift;
warn "trackname_from_url($url)" if DEBUG;
my $filename = $self->escape_url($url, $uniquefy);
my $path = $self->blind_track_path($filename);
rmtree($path) if -e $path; # only happens if uniquefy = 0
mkpath $path;
return $filename;
}
# Max filename () - Returns the maximum possible length for a file name.
sub max_filename {
my $self = shift;
my $length = POSIX::pathconf($self->path, &POSIX::_PC_NAME_MAX) || 255;
return $length - 4; # give enough room for the suffix
}
# Import URL (URL, Overwrite[, Privacy Policy]) - Imports a URL for use in the database.
sub import_url {
my $self = shift;
my $url = shift;
my $overwrite = shift;
my $privacy_policy = shift || "private";
my $username = $self->{username};
my $key;
if ($url =~ m!http://([^/]+).+/(\w+)/\?.*t=([^+;]+)!) {
my @tracks = split /\+/,$3;
$key = "Shared track from $1 (@tracks)";
} else {
$key = "Shared track from $url";
}
my $filename = $self->trackname_from_url($url, !$overwrite);
my $file = $self->add_file($filename, 1);
my $loader = Bio::Graphics::Browser2::DataLoader->new($filename,
$self->track_path($file),
$self->track_conf($file),
$self->{config},
$self->uploadsid);
$loader->strip_prefix($self->{config}->seqid_prefix);
$loader->set_status('starting import');
my $conf = $self->track_conf($file);
open (my $f, "+>", $conf) or croak "Couldn't open $conf: $!";
my @data = $f;
if ($url =~ /\.bam$/) {
print $f $self->remote_bam_conf($file, $url, $key);
}
elsif ($url =~ /\.bw$/) {
print $f $self->remote_bigwig_conf($file, $url, $key);
}
elsif ($url =~ /\.bb$/) {
print $f $self->remote_bigbed_conf($file, $url, $key);
}
else {
print $f $self->remote_mirror_conf($file, $url, $key);# Conf Metadata (File) - Returns the modified time and size of a track's configuration file.
}
close $f;
open my $i, ">", $self->import_flag($file);
close $i;
$loader->set_processing_complete;
return (1, '', [$filename], $file);
}
# Reload File (File) - Attempts to reload a file into the database.
sub reload_file {
my $self = shift;
my $file = shift;
my $filename = $self->filename($file);
my @sources = $self->source_files($file);
for my $s (@sources) {
my ($name, $size, $mtime, $path) = @$s;
rename $path, "$path.bak" or next;
my $io = IO::File->new("$path.bak") or next;
my ($result) = $self->upload_file($name, $io,'',1);
if ($result) {
unlink "$path.bak";
} else {
rename "$path.bak", $path;
}
}
}
# Mirror URL (Filename, URL, Overwrite?) - Attempts to "mirror" a file on a URL, to avoid fetching it every time.
sub mirror_url {
my $self = shift;
my ($filename,$url,$overwrite,$render) = @_;
warn "mirroring..." if DEBUG;
if ($url =~ /\.(bam|bw|bb)$/ or $url =~ /\b(gbgff|das)\b/) {
return $self->import_url($url, $overwrite);
}
# first we do a HEAD to validate that the thing exists
eval "require LWP::UserAgent" unless LWP::UserAgent->can('new');
my $agent = LWP::UserAgent->new();
my $result = $agent->head($url);
unless ($result->is_success) {
my $msg = $url.': '.$result->status_line;
warn $msg if DEBUG;
return (0,$msg,[]);
}
# fetch in one process, process in another
eval "use IO::Pipe" unless IO::Pipe->can('new');
my $fh = IO::Pipe->new;
my $child = $render->fork();
die "Couldn't fork" unless defined $child;
unless ($child) {
warn "printing from child..." if DEBUG;
$fh->writer();
$self->_print_url($agent, $url, $fh);
CORE::exit 0;
}
$fh->reader;
$filename = $self->trackname_from_url($filename, !$overwrite);
my $content_type = $result->header('Content-Type') || 'text/plain';
if ($content_type eq 'application/gzip' or $filename =~ /\.gz$/) {
$fh = $self->install_filter($fh,'gunzip -c');
} elsif ($content_type eq 'application/bzip2' or $filename =~ /\.bz2$/) {
$fh = $self->install_filter($fh,'bunzip2 -c');
}
my $file = $self->add_file($filename, 1);
# guess the file type from the first non-blank line
my ($type, $lines, $eol) = $self->guess_upload_type($file, $fh);
$lines ||= [];
my (@tracks, $fcgi);
my $mirror_result = eval {
local $SIG{TERM} = sub { die "cancelled" };
croak "Could not guess the type of the file $filename" unless $type;
my $load = $self->get_loader($type, $file);
$load->eol_char($eol);
@tracks = $load->load($lines, $fh);
1;
};
if ($@ =~ /cancelled/) {
$self->delete_file($file);
return (0,'Upload process terminated',[]);
}
my $msg = $@;
warn "UPLOAD ERROR: ", $msg if $msg;
$self->delete_file($file) unless $mirror_result;
$self->set_mirrored($file, $url);
return ($mirror_result, $msg, \@tracks);
}
# Upload Data (Filename, Data, Content Type, Overwrite?) - Uploads a string of data entered as text (on the Upload & Share Tracks tab).
sub upload_data {
my $self = shift;
my ($file_name, $data, $content_type, $overwrite) = @_;
my $io = IO::String->new($data);
$self->upload_file($file_name, $io, $content_type, $overwrite);
}
# Upload URL (URL) - Uploads a file at a specified URL to the database.
sub upload_url {
my $self = shift;
my $url = shift;
my $dir = tempdir(CLEANUP=>1);
my $path = File::Spec->catfile($dir, basename($url));
eval "require LWP::UserAgent" unless LWP::UserAgent->can('new');
my $agent = LWP::UserAgent->new();
my $response = $agent->mirror($url, $path);
$response->is_success or die $response->status_line;
my $mime = $response->header('Content-type');
open my $fh,"<",$path;
my @args = $self->upload_file(basename($url),$fh,$mime,1);
unlink $path;
File::Temp::cleanup();
return @args;
}
# Upload File (Filename, File Handle, Content Type, Overwrite?) - Uploads a user's file, as called by the AJAX upload system (on the Upload & Share Tracks tab) via Action.pm.
sub upload_file {
my $self = shift;
my ($file_name, $fh, $content_type, $overwrite) = @_;
my $original_name = $file_name;
$file_name =~ s/\.(gz|bz2)$//; # to indicate that it is decompressed
$file_name =~ s/\.(tgz|tbz|tbz2)$/.tar/; # change archive extension
warn "$file_name: OVERWRITE = $overwrite" if DEBUG;
my $filename = $self->trackname_from_url($file_name, !$overwrite);
$content_type ||= '';
if ($original_name =~ /\.sam\.gz/) { # special case compressed sam files - do not uncompress!
# $filename = $original_name;
} elsif ($content_type eq 'application/gzip' or $original_name =~ /\.(?:tgz|gz)$/) {
$fh = $self->install_filter($fh,'gunzip -c');
} elsif ($content_type eq 'application/bzip2' or $original_name =~ /\.(?:tbz2?|bz2)$/) {
$fh = $self->install_filter($fh,'bunzip2 -c');
}
my $fileid = $self->get_file_id($filename) if $self->database;
my $file = $self->database ? $fileid ? $fileid : $self->add_file($filename) : $filename;
# guess the file type from the first non-blank line
my ($type, $lines, $eol) = $self->guess_upload_type($file, $fh);
$lines ||= [];
my (@tracks, $fcgi);
my $result = eval {
local $SIG{TERM} = sub { die "cancelled" };
croak "Could not guess the type of the file $file_name" unless $type;
croak "This server does not support $type uploads"
if $type =~ /bigwig|bigbed|useq|archive/ && !$self->has_bigwig;
my $load = $self->get_loader($type, $file);
$load->eol_char($eol);
@tracks = $load->load($lines, $fh);
1;
};
if ($@ =~ /cancelled/) {
$self->delete_file($file);
return (0,'Cancelled by user',[]);
}
my $msg = $@;
warn "UPLOAD ERROR: ", $msg if $msg;
if ($result) {
$self->title($file,$filename);
} else {
$self->delete_file($file);
}
return ($result, $msg, \@tracks);
}
# set title of upload- implemented in subclasses
sub title { }
# set the key of a labeled track - this involves rewriting the track config
sub set_key {
my $self = shift;
my ($file,$label,$new_key) = @_;
my $old_path = $self->track_conf($file);
my $new_path = "$old_path.bak";
open my $in,$old_path or croak "$old_path: $!";
open my $out,'>',$new_path or croak "$new_path: $!";
my $in_stanza;
while (<$in>) {
$in_stanza = undef if /^\[.+\]/;
$in_stanza ||= /^\[$label\]/;
next unless $in_stanza;
$_ = "key = $new_key\n" if /^key/;
} continue {
print $out $_;
}
close $in; close $out;
rename $new_path,$old_path;
return $new_key; # in case we want to change it later
}
# Merge Conf (File, New Data) - Merges new data into a track's configuration file.
sub merge_conf {
my $self = shift;
my $file = shift;
my $filename = $self->filename($file);
my $new_data = shift;
my $path = $self->track_conf($file) or return;
my @lines = split /\r\n|\r|\n/,$new_data;
my (%stanzas, $current_stanza);
for (@lines) {
if (/^\[(.+)\]/) {
$current_stanza = $1;
$stanzas{$current_stanza} = '';
} elsif ($current_stanza) {
$stanzas{$current_stanza} .= "$_\n";
}
}
open my $fh, $path or croak "$path: $!";
my $merged = '';
my @database;
# read header with the [database] definition
while (<$fh>) {
$merged .= $_;
push @database,$1 if /^\[(.+):database/;
last if /cut here/;
}
$merged .= "\n";
# read the rest
while (<$fh>) {
if (/^\[/) {
(my $stanza = $_) =~ s/\[(\w+?)_.+_(\d+)(:\d+)?\]\s*\n/$1_$2$3/;
$stanza =~ s/^\[//; # just in case
$stanza =~ s/\]\n//;
if (my $body = $stanzas{$stanza}) {
$merged .= $_;
$merged .= $body;
delete $stanzas{$stanza};
}
}
}
# anything that's left (new stuff)
for my $stanza (keys %stanzas) {
$merged .= "\n[$stanza]\n";
$merged .= $stanzas{$stanza};
}
$merged =~ s/database_(\d+).+/$database[$1]/g;
open $fh,'>',$path or croak "Can't open $path for writing: $!";
print $fh $merged;
close $fh;
}
#Labels (File) - Returns the track labels for a specified uploaded file.
sub labels {
my $self = shift;
my $file = shift;
my $conf = $self->track_conf($file);
return grep {!/:(database|\d+)/} eval{ Bio::Graphics::FeatureFile->new(-file=>$conf)->labels };
}
sub file_from_label {
my $self = shift;
my $label = shift;
my %track_lookup = $self->track_lookup;
return $track_lookup{$label};
}
# Status (File) - Returns the status of a DataLoader object for a specific file.
sub status {
my $self = shift;
my $file = shift;
my $filename = $self->filename($file);
my $loader = 'Bio::Graphics::Browser2::DataLoader';
my $load = $loader->new($filename,
$self->track_path($file),
$self->track_conf($file),
$self->{config},
$self->uploadsid,
);
return $load->get_status();
}
# Get Loader (Type, File) - Returns the loader of the appropriate DataLoader package type for a specific file.
sub get_loader {
my $self = shift;
my $type = shift;
my $file = shift;
my $filename = $self->filename($file);
my $module = "Bio::Graphics::Browser2::DataLoader::$type";
eval "require $module";
die $@ if $@;
my $loader = $module->new($filename,
$self->track_path($file),
$self->track_conf($file),
$self->{config},
$self->uploadsid,
);
$loader->strip_prefix($self->{config}->seqid_prefix);
$loader->force_category('General') if $self->is_admin;
return $loader;
}
# guess the file type and eol based on its name and the first 1024 bytes
# of the file. The @$lines will contain the lines that were consumed
# during this operation so that the info isn't lost.
sub guess_upload_type {
my $self = shift;
my ($type, $lines, $eol) = $self->_guess_upload_type(@_);
$type = 'wig2bigwig' if $type eq 'wiggle' && $self->has_bigwig;
return ($type, $lines, $eol);
}
sub _guess_upload_type {
my $self = shift;
my $file = shift;
my $filename = $self->filename($file);
my $fh = shift;
my $buffer;
read($fh,$buffer,1024);
# first check for binary upload
my $magic = substr($buffer,0,4);
return ('bam',[$buffer],undef)
if $magic eq "\x1f\x8b\x08\x04";
return('bigwig',[$buffer],undef)
if $magic eq "\x26\xfc\x8f\x88";
return('bigbed',[$buffer],undef)
if $magic eq "\xeb\xf2\x89\x87";
if ($magic eq "\x50\x4B\x03\x04") { # zip file
return('useq', [$buffer], undef) if $filename =~ /\.useq$/i;
return('archive', [$buffer], undef) if $filename =~ /\.zip$/i;
}
# check for archives
return('archive', [$buffer], undef) if
$filename =~ /\.(?:tar|tgz|tbz|tbz2)(?:\.(gz|bz2))?$/i;
# everything else is text (for now)
my $eol = $buffer =~ /\015\012/ ? "\015\012" # MS-DOS
:$buffer =~ /\015/ ? "\015" # Macintosh
:$buffer =~ /\012/ ? "\012" # Unix
:"\012"; # default to Unix
local $/ = $eol;
my @lines = map {$_.$eol} split $eol,$buffer;
$lines[-1] =~ s/$eol$// unless $buffer =~ /$eol$/;
my $remainder = <$fh>;
$remainder ||= '';
$lines[-1] .= $remainder;
# first guess based on file names
my $ftype = $filename =~ /\.gff(\.(gz|bz2|Z))?$/i ? 'gff'
:$filename =~ /\.gff3(\.(gz|bz2|Z))?$/i ? 'gff3'
:$filename =~ /\.bed(\.(gz|bz2|Z))?$/i ? 'bed'
:$filename =~ /\.bw$/i ? 'bigwig'
:$filename =~ /\.bb$/i ? 'bigbed'
:$filename =~ /\.wig(\.(gz|bz2|Z))?$/i ? 'wiggle'
:$filename =~ /\.fff(\.(gz|bz2|Z))?$/i ? 'featurefile'
:$filename =~ /\.bam(\.gz)?$/i ? 'bam'
:$filename =~ /\.sam(\.gz)?$/i ? 'sam'
:$filename =~ /\.useq$/i ? 'useq'
:$filename =~ /\.(?:tar|tgz|tbz|tbz2|zip)(?:\.(gz|bz2))?$/i ? 'archive'
:undef;
return ($ftype,\@lines,$eol) if $ftype;
# otherwise scan the thing until we find a pattern we know about
# or hit the end of the file. Extra lines that we read are
# accumulated into the @lines array.
my $i = 0;
while (1) {
my $line;
if ($i <= $#lines) {
$line = $lines[$i++];
} else {
my $line = <$fh>;
last unless $line;
push @lines,$line;
}
return ('featurefile',\@lines,$eol) if $line =~ /^reference/i;
return ('featurefile',\@lines,$eol) if $line =~ /\w+:\d+\.\.\d+/i;
return ('gff2',\@lines,$eol) if $line =~ /^\#\#gff-version\s+2/;
return ('gff3',\@lines,$eol) if $line =~ /^\#\#gff-version\s+3/;
return ('wiggle',\@lines,$eol) if $line =~ /type=wiggle/;
return ('bed',\@lines,$eol) if $line =~ /^track type/;
return ('bed',\@lines,$eol) if $line =~ /^\w+\s+\d+\s+\d+/;
return ('sam',\@lines,$eol) if $line =~ /^\@[A-Z]{2}/;
return ('sam',\@lines,$eol) if $line =~ /^[^ \t\n\r]+\t[0-9]+\t[^ \t\n\r@=]+\t[0-9]+\t[0-9]+\t(?:[0-9]+[MIDNSHP])+|\*/;
}
return;
}
sub has_bigwig {
my $self = shift;
return $HASBIGWIG if defined $HASBIGWIG;
return $HASBIGWIG = 1 if Bio::DB::BigWig->can('new');
my $result = eval "require Bio::DB::BigWig; 1";
return $HASBIGWIG = $result || 0;
}
# Install Filter (File Handle, Command) - Attaches a filter (such as GUNZIP or BUNZIP2) to a file handle.
sub install_filter {
my $self = shift;
my ($in_fh, $command) = @_;
my $child = open(my $out_fh,"-|");
defined $child or die "Couldn't fork for pipe: $!";
return $out_fh if $child;
# we are in child now
Bio::Graphics::Browser2::Render->prepare_fcgi_for_fork('child');
my $unzip = IO::File->new("| $command") or die "Can't open $command: $!";
my $buffer;
while ((my $bytes = read($in_fh,$buffer,8192))>0) {
$unzip->print($buffer);
}
close $unzip;
CORE::exit 0;
}
sub remote_mirror_conf {
my $self = shift;
my $file = shift;
my $filename = $self->filename($file);
my ($url, $key) = @_;
return <<END;
>>>>>>>>>>>>>> cut here <<<<<<<<<<<<
[$filename]
remote feature = $url
category = My Tracks:Remote Tracks
key = $key
END
;
}
sub remote_bam_conf {
my $self = shift;
my $file = shift;
my $filename = $self->filename($file);
my ($url, $key) = @_;
my $id = rand(1000);
my $dbname = "remotebam_$id";
my $track_id = $filename;# . "_1";
eval "require Bio::Graphics::Browser2::DataLoader::bam"
unless Bio::Graphics::Browser2::DataLoader::bam->can('new');
my $loader = Bio::Graphics::Browser2::DataLoader::bam->new(
$filename,
$self->track_path($file),
$self->track_conf($file),
$self->{config},
$self->uploadsid);
my $fasta = $loader->get_fasta_file;
return <<END;
[$dbname:database]
db_adaptor = Bio::DB::Sam
db_args = -bam $url
-split_splices 1
-fasta $fasta
search options = none
>>>>>>>>>>>>>> cut here <<<<<<<<<<<<
[$track_id:2001]
feature = coverage:2000
min_score = 0
glyph = wiggle_xyplot
height = 50
fgcolor = black
bgcolor = black
autoscale = local
[$track_id]
database = $dbname
feature = read_pair
glyph = segments
feature_limit = 500
draw_target = 1
show_mismatch = 1
mismatch_color = red
bgcolor = blue
fgcolor = blue
height = 3
label = 1
label density = 50
stranded = 1
bump = fast
key = $key
END
;
}
sub remote_bigwig_conf {
my $self = shift;
my $file = shift;
my $filename = $self->filename($file);
my ($url, $key) = @_;
my $id = rand(1000);
my $dbname = "remotebw_$id";
my $track_id = $filename;
warn "remote_bigwig_conf";
return <<END;
[$dbname:database]
db_adaptor = Bio::DB::BigWig
db_args = -bigwig $url
search options = none
>>>>>>>>>>>>>> cut here <<<<<<<<<<<<
[$track_id]
database = $dbname
feature = summary
glyph = wiggle_whiskers
max_color = lightgrey
min_color = lightgrey
mean_color = black
stdev_color = grey
stdev_color_neg = grey
height = 20
END
}
sub remote_bigbed_conf {
my $self = shift;
my $file = shift;
my $filename = $self->filename($file);
my ($url, $key) = @_;
my $id = rand(1000);
my $dbname = "remotebb_$id";
my $track_id = $filename;
my @COLORS = qw(blue red orange brown mauve peach
green cyan yellow coral);
my $color = $COLORS[rand @COLORS];
warn "remote_bigbed_conf";
return <<END;
[$dbname:database]
db_adaptor = Bio::DB::BigBed
db_args = -bigbed $url
search options = none
>>>>>>>>>>>>>> cut here <<<<<<<<<<<<
[$track_id]
database = $dbname
feature = region
glyph = segments
label density = 50
feature_limit = 500
bump = fast
stranded = 1
height = 4
bgcolor = $color
fgcolor = $color
key = $filename segments
description =
[$track_id\_coverage]
database = $dbname
feature = summary
glyph = wiggle_whiskers
fgcolor = black
height = 50
autoscale = chromosome
key = $filename coverage
description =
END
}
sub _print_url {
my $self = shift;
my ($agent, $url, $fh) = @_;
$agent->get($url,':content_cb' => sub { print $fh shift; });
}
# Sharing Link (File ID) - Generates the sharing link for a specific file.
sub sharing_link {
my $self = shift;
my $file = shift or return;
return CGI::url(-full => 1, -path_info => 1) . "?share_link=" . $file;
}
# These methods are replaced by methods in Filesystem.pm and Database.pm
# Many of these functions are called asynchronously, if you want to connect an AJAX call to one of these functions add a hook in Action.pm
sub get_file_id { warn "get_file_id() has been called, without properly inheriting subclass Database.pm"; }
sub filename { warn "filename() has been called, without properly inheriting a subclass (like Filesystem.pm or Database.pm)"; }
sub nowfun { warn "nowfun() has been called, without properly inheriting subclass Database.pm"; }
sub get_uploaded_files { warn "get_uploaded_files() has been called, without properly inheriting a subclass (like Filesystem.pm or Database.pm)"; }
sub get_public_files { warn "get_public_files() has been called, without properly inheriting subclass Database.pm"; }
sub get_imported_files { warn "get_imported_files() has been called, without properly inheriting a subclass (like Filesystem.pm or Database.pm)"; }
sub get_shared_files { warn "get_shared_files() has been called, without properly inheriting subclass Database.pm"; }
sub share { warn "share() has been called, without properly inheriting subclass Database.pm"; }
sub unshare { warn "unshare() has been called, without properly inheriting subclass Database.pm"; }
sub field { warn "field() has been called, without properly inheriting subclass Database.pm"; }
sub update_modified { warn "update_modified() has been called, without properly inheriting subclass Database.pm"; }
sub created { warn "created() has been called, without properly inheriting a subclass (like Filesystem.pm or Database.pm)"; }
sub modified { warn "modified() has been called, without properly inheriting a subclass (like Filesystem.pm or Database.pm)"; }
sub description { warn "description() has been called, without properly inheriting a subclass (like Filesystem.pm or Database.pm)"; }
sub add_file { warn "add_file() has been called, without properly inheriting a subclass (like Filesystem.pm or Database.pm)"; }
sub delete_file { warn "delete_file() has been called, without properly inheriting a subclass (like Filesystem.pm or Database.pm)"; }
sub is_imported { warn "is_imported() has been called, without properly inheriting a subclass (like Filesystem.pm or Database.pm)"; }
sub permissions { warn "permissions() has been called, without properly inheriting subclass Database.pm"; }
sub is_mine { warn "is_mine() has been called, without properly inheriting a subclass (like Filesystem.pm or Database.pm)"; }
sub owner { warn "owner() has been called, without properly inheriting a subclass (like Filesystem.pm or Database.pm)"; }
sub is_shared_with_me { warn "is_shared_with_me() has been called, without properly inheriting subclass Database.pm"; }
sub file_type { warn "file_type() has been called, without properly inheriting a subclass (like Filesystem.pm or Database.pm)"; }
sub shared_with { warn "shared_with() has been called, without properly inheriting subclass Database.pm"; }
sub file_exists { warn "file_exists() has been called, without properly inheriting subclass Filesystem.pm"; }
sub change_userid { warn "change_userid() has been called, without properly inheriting a subclass (like Filesystem.pm or Database.pm)"; }
sub change_uploadsid { warn "change_uploadsid() has been called, without properly inheriting a subclass (like Filesystem.pm or Database.pm)"; }
sub close_database { } # do nothing
package Bio::Graphics::Browser2::UserConf;
use base 'Tie::Handle';
use Symbol;
sub fh {
my $class = shift;
my $path = shift;
my $g = gensym;
tie(*$g,$class,$path);
return $g;
}
sub TIEHANDLE {
my $class = shift;
my $path = shift;
open my $f,$path or die "$path:$!";
return bless {
fh => $f,
seen_cut => 0,
_db => {}, # remember db names
-didx => 0, # db indexes
},$class;
}
sub READLINE {
my $self = shift;
my $fh = $self->{fh};
while (my $line = <$fh>) {
$self->{_db}{$1} = $self->{_didx}++ if $line =~ /^\[(.+):database/;
if ($line =~ /cut here/i) {
$self->{seen_cut}++;
next;
}
next unless $self->{seen_cut};
$line =~ s/database\s*=\s*(.+)/database = database_$self->{_db}{$1} # do not change this!/;
$line =~ s/\[(\w+?)_.+_(\d+)(:\d+)?\]/[$1_$2$3]/;
return $line;
}
return;
}
sub CLOSE { close shift->{fh} }
# this is for the administrator-uploaded tracks.
# we simply change the location of the uploads
package Bio::Graphics::Browser2::AdminTracks;
use base 'Bio::Graphics::Browser2::UserTracks';
sub admin {1}
1;
|