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# GenomeTester4 package
Copyright (C) University of Tartu 2015-2017
Please cite: Kaplinski L, Lepamets M, Remm M. (2015). GenomeTester4: a toolkit for performing basic set operations – union, intersection and complement on k-mer lists. GigaScience, 4:58.
GenomeTester4 is a toolkit for creating and manipulating k-mer lists. It
contains 3 programs: glistmaker, glistcompare and glistquery. It is
developed by Department of Bioinformatics, University of Tartu and
distributed under GPL version 3.0 (or later).
1. Quick usage for the impatient
```
glistmaker|glistcompare|glistquery -h
```
Prints out quick description of command line arguments
```
glistmaker FASTA_FILES -w WORD_LENGTH -o OUTPUT_NAME
```
Generates a list of all unique k-mers in input files with their frequencies. The output file is
named OUTPUT_NAME_WORD_LENGTH_0_0.list
```
glistquery LIST_FILE
```
Prints out all k-mers and their frequencies in list file
```
glistquery LIST_FILE -q WORD
```
Prints out the frequency of given word in list file
```
glistquery LIST_FILE -f WORD_FILE
```
Prints out the frequencies of all words in WORD_FILE in list file
```
glistcompare LIST_FILE_1 LIST_FILE_2 --union -o OUTPUT_NAME
```
Generates union of all words in both input lists. The frequencies in final
list are sums of both input frequencies.
```
glistcompare LIST_FILE_1 LIST_FILE_2 --intersection -o OUTPUT_NAME
```
Generates intersection of words in both input lists. The frequencies in
final list are smaller frequencies of either list
```
glistcompare LIST_FILE_1 LIST_FILE_2 --difference -o OUTPUT_FILE
```
Generates complement (words in the first file and NOT in second file) of
words in both input lists. The frequencies in final list are the original
frequencies in the first list.
2. Compiling
GenomeTester4 is written in standard C. The only external dependency should
be pthreads library that is standard in all Linux systems.
Binaries compiled with full optimization are included in directory 'bin'.
If you for whatever reason have to compile these manually, just enter into
subdirectory 'src' and type:
```
make clean
make
```
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