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 | #include "SCFtype.h"
// Stuff copied from staden package
/*
 * Basic type definitions
 */
typedef unsigned long int   uint_4;
typedef signed   long int    int_4;
typedef unsigned short int uint_2;
typedef signed   short int  int_2;
typedef unsigned char  uint_1;
typedef signed   char   int_1;
/**	\typedef SCF_Header
	\brief Type definition for the SCF_Header structure
 */
typedef struct {
    uint_4 magic_number;
    uint_4 samples;          ///< Number of elements in Samples matrix 
    uint_4 samples_offset;   ///< Byte offset from start of file 
    uint_4 bases;            ///< Number of bases in Bases matrix 
    uint_4 bases_left_clip;  ///< OBSOLETE: No. bases in left clip (vector) 
    uint_4 bases_right_clip; ///< OBSOLETE: No. bases in right clip (qual) 
    uint_4 bases_offset;     ///< Byte offset from start of file 
    uint_4 comments_size;    ///< Number of bytes in Comment section 
    uint_4 comments_offset;  ///< Byte offset from start of file 
    char version[4];         ///< "version.revision", eg '3' '.' '0' '0' 
    uint_4 sample_size;      ///< Size of samples in bytes 1=8bits, 2=16bits
    uint_4 code_set;         ///< code set used (but ignored!)
    uint_4 private_size;     ///< No. of bytes of Private data, 0 if none 
    uint_4 private_offset;   ///< Byte offset from start of file 
    uint_4 spare[18];        ///< Unused 
} SCF_Header;
/**	\typedef SCF_Samples1
	\brief Type definition for the SCF_Samples1 data
 */
typedef struct {
        uint_1 sample_A;           ///< Sample for A trace 
        uint_1 sample_C;           ///< Sample for C trace 
        uint_1 sample_G;           ///< Sample for G trace 
        uint_1 sample_T;           ///< Sample for T trace 
} SCF_Samples1;
/**	\typedef SCF_Samples2
	\brief Type definition for the SCF_Samples2 data
 */
typedef struct {
        uint_2 sample_A;           ///< Sample for A trace 
        uint_2 sample_C;           ///< Sample for C trace 
        uint_2 sample_G;           ///< Sample for G trace 
        uint_2 sample_T;           ///< Sample for T trace 
} SCF_Samples2 ;
/*
 * Type definition for the sequence data
 */
typedef struct {
    uint_4 peak_index;        /* Index into Samples matrix for base posn */
    uint_1 prob_A;            /* Probability of it being an A */
    uint_1 prob_C;            /* Probability of it being an C */
    uint_1 prob_G;            /* Probability of it being an G */
    uint_1 prob_T;            /* Probability of it being an T */
    char   base;              /* Called base character        */
    uint_1 spare[3];          /* Spare */
} SCF_Base;
// GENtle stuff
uint_4 get_real_uint4 ( uint_4 x )
	{
	unsigned char *c = (unsigned char*) (&x) ;
	uint_4 u = 0 ;
	u |= *(c+0) ;
	u <<= 8 ;
	u |= *(c+1) ;
	u <<= 8 ;
	u |= *(c+2) ;
	u <<= 8 ;
	u |= *(c+3) ;
	return u ;
	}
void make_real_uint4 ( uint_4 &x )
	{
	x = get_real_uint4 ( x ) ;
	}
	
uint_2 get_real_uint2 ( uint_2 x )
	{
	unsigned char *c = (unsigned char*) (&x) ;
	uint_2 ret = 0 ;
	ret |= *(c+1) ;
	ret <<= 8 ;
	ret |= *(c+0) ;
	return ret ;
	}
SCFtype::SCFtype ()
	{
	}
bool SCFtype::parse ( wxString filename )
	{
	wxFile f ( filename , wxFile::read ) ;
	long l = f.Length() ;
	unsigned char *t = new unsigned char [l+15] ;
	f.Read ( t , l ) ;
	f.Close() ;
	
	if ( *(t+0) != '.' || *(t+1) != 's' || *(t+2) != 'c' || *(t+3) != 'f' )
		{
		delete t ;
		return false ; // No luck
		}
	
	SCF_Header *header = (SCF_Header*) t ;
	wxString version ;
	version += (*header).version[0] ;
	version += (*header).version[1] ;
	version += (*header).version[2] ;
	version += (*header).version[3] ;
	if ( (*header).version[0] < '3' )
		{
		wxMessageBox ( _T("Cannot read SFC prior to 3.0, this is ") + version ) ;
		return false ;
		}
	// Requires SCF V3.0 from here on!
	make_real_uint4 ( (*header).comments_size ) ;
	make_real_uint4 ( (*header).comments_offset ) ;
	make_real_uint4 ( (*header).bases ) ;
	make_real_uint4 ( (*header).bases_offset ) ;
	make_real_uint4 ( (*header).samples ) ;
	make_real_uint4 ( (*header).samples_offset ) ;
	make_real_uint4 ( (*header).sample_size ) ;
	
	if ( (*header).samples == 0 || (*header).bases == 0 ) return false ; // Paranoia
	unsigned char *c ;
	unsigned int a ;
	
	// Read comment
	sd.comment.Empty() ;
	for ( a = 0 , c = t + (*header).comments_offset ; a < (*header).comments_size ; a++ , c++ )
		sd.comment += *c ;
	
	// Preparing to read sequence information
	void *v = t + (*header).bases_offset ;
	unsigned long *ulp ;
	sd.sequence.clear () ;
	sd.seq.Empty () ;
	for ( a = 0 , ulp = (unsigned long*) v ; a < (*header).bases ; a++ , ulp++ )
		{
		TSequencerDataSequenceItem si ;
		si.peak_index = get_real_uint4 ( *ulp ) ;
		sd.sequence.push_back ( si ) ;
		}
	// Reading sequence data
	for ( a = 0 ; a < 5 ; a++ )
		{
		v = t + (*header).bases_offset + (*header).bases * ( 4 + a ) ;
		read_data_block ( v , a ) ;
		}
	// Sorting sequence data; might be unnecessary
	for ( a = 1 ; a < sd.sequence.size() ; a++ )
		{
		if ( sd.sequence[a-1].peak_index <= sd.sequence[a].peak_index ) continue ;
		TSequencerDataSequenceItem d = sd.sequence[a-1] ;
		sd.sequence[a-1] = sd.sequence[a] ;
		sd.sequence[a] = d ;
		a = 0 ;
		}
/*	
	// Maximum trace data
	unsigned long min = 9999 , max = 0 ;
	for ( a = 0 ; a < sd.sequence.size() ; a++ )
		{
		if ( max < sd.sequence[a].peak_index ) max = sd.sequence[a].peak_index ;
		if ( min > sd.sequence[a].peak_index ) min = sd.sequence[a].peak_index ;
		}
	if ( max >= sd.tracer.size() ) return false ; // Paranoia
*/
	// Read tracer data
	sd.tracer.clear () ;
	while ( sd.tracer.size() < (*header).samples )
		sd.tracer.push_back ( TSequencerDataTracerItem () ) ;
	for ( a = 0 ; a < 4 ; a++ )
		{
		unsigned long offset = (*header).samples_offset + (*header).samples * a * (*header).sample_size ;
		v = t + offset ;
		read_tracer_block ( v , a , (*header).sample_size ) ;
		}
	// Fix tracer diff diff hell
	for ( a = 0 ; a < 4 ; a++ )
		fix_diff ( a ) ;
	// Setup alternative tracer data set
	for ( a = 0 ; a < 4 ; a++ ) sd.tracer2[a].clear () ;
	for ( a = 0 ; a < sd.tracer.size() ; a++ )
		{
		TSequencerDataTracerItem ti = sd.tracer[a] ;
		sd.tracer2[TRACER_ID_A].push_back ( ti.data[0] ) ;
		sd.tracer2[TRACER_ID_C].push_back ( ti.data[1] ) ;
		sd.tracer2[TRACER_ID_G].push_back ( ti.data[2] ) ;
		sd.tracer2[TRACER_ID_T].push_back ( ti.data[3] ) ;
		}
	wxFile out ( _T("C:\\test.txt") , wxFile::write ) ;
	for ( a = 0 ; a < sd.sequence.size() ; a++ )
	{
		out.Write ( wxString::Format ( _T("%5d: %5d %c\r\n") , a , sd.sequence[a].peak_index , sd.sequence[a].base ) ) ;
    }
/*	
	// THE FOLLOWING MESS MIGHT PROVE USEFUL ONE DAY
	// TO IMPORT SCF VERSIONS 1 AND 2 AS WELL
	v += (*header).bases * 4
	for ( a = 0 , base = (SCF_Base*) v ; a < (*header).bases ; a++ , base++ )
		{
		TSequencerDataSequenceItem si = sd[a] ;
//		si.peak_index = get_real_uint4 ( (*base).peak_index ) ;
		si.prob_a = (*base).prob_A ;
		si.prob_c = (*base).prob_C ;
		si.prob_g = (*base).prob_G ;
		si.prob_t = (*base).prob_T ;
		
		char c = 'A' ;
		int prob = si.prob_a ;
		if ( prob < si.prob_c ) { prob = si.prob_c ; c = 'C' ; }
		if ( prob < si.prob_g ) { prob = si.prob_g ; c = 'G' ; }
		if ( prob < si.prob_t ) { prob = si.prob_t ; c = 'T' ; }
		sd.seq += c ;
		
		si.base = (*base).base ;
		sd[a] = si ;
//		sd.sequence.push_back ( si ) ;
//		out += wxString::Format ( _T("%d:%c\n\r\n") , si.peak_index , si.base ) ;
		}
	// Read samples
	SCF_Samples1 *s1 = NULL ;
	SCF_Samples2 *s2 = NULL ;
	v = t + (*header).samples_offset ;
	bool use_word = (*header).sample_size == 2 ;
	if ( use_word ) s2 = (SCF_Samples2 *) v ;
	else s1 = (SCF_Samples1 *) v ;
	sd.tracer.clear () ;
	for ( a = 0 ; a < 4 ; a++ ) sd.tracer2[a].clear () ;
	for ( a = 0 ; a < (*header).samples ; a++ )
		{
		TSequencerDataTracerItem ti ;
		ti.a = use_word ? get_real_uint2 ( s2->sample_A ) : s1->sample_A ;
		ti.c = use_word ? get_real_uint2 ( s2->sample_C ) : s1->sample_C ;
		ti.g = use_word ? get_real_uint2 ( s2->sample_G ) : s1->sample_G ;
		ti.t = use_word ? get_real_uint2 ( s2->sample_T ) : s1->sample_T ;
//		out += wxString::Format ( _T("%d,%d,%d,%d; ") , ti.a , ti.c , ti.g , ti.t ) ;
		sd.tracer.push_back ( ti ) ;
		sd.tracer2[TRACER_ID_A].push_back ( ti.a ) ;
		sd.tracer2[TRACER_ID_C].push_back ( ti.c ) ;
		sd.tracer2[TRACER_ID_G].push_back ( ti.g ) ;
		sd.tracer2[TRACER_ID_T].push_back ( ti.t ) ;
		if ( use_word ) s2++ ;
		else s1++ ;
		}
	for ( a = 1 ; a < sd.sequence.size() ; a++ )
		{
		if ( sd.sequence[a-1].peak_index <= sd.sequence[a].peak_index ) continue ;
		TSequencerDataSequenceItem d = sd.sequence[a-1] ;
		sd.sequence[a-1] = sd.sequence[a] ;
		sd.sequence[a] = d ;
		a = 0 ;
		}
*/
//	wxMessageBox ( out ) ;
//	wxMessageBox ( sd.comment ) ;
	
	delete t ;
	return true ;
	}
void SCFtype::read_tracer_block ( void *v , unsigned int mode , unsigned int sample_size )
	{
	unsigned int a , samples = sd.tracer.size() ;
	unsigned char *c = (unsigned char*) v ;
	unsigned long lastx = 0 ;
	for ( a = 0 ; a < samples ; a++ )
		{
		unsigned long x = 0 ;
		if ( sample_size == 2 )
			{
			x |= (unsigned char) *(c+0) ;
			x <<= 8 ;
			x |= (unsigned char) *(c+1) ;
			
			if ( x >= 32768 ) {
                x = x - 65536 ;
            }
			
			c += 2 ;
			}
		else x = *c++ ;
        sd.tracer[a].data[mode] = x ;
		}
	}
void SCFtype::read_data_block ( void *v , unsigned int mode )
	{
	unsigned int a , bases = sd.sequence.size() ;
	unsigned char *c = (unsigned char*) v ;
	for ( a = 0 ; a < bases ; a++ , c++ )
		{
		switch ( mode )
			{
			case 0 : sd.sequence[a].prob_a = *c ; break ;
			case 1 : sd.sequence[a].prob_c = *c ; break ;
			case 2 : sd.sequence[a].prob_g = *c ; break ;
			case 3 : sd.sequence[a].prob_t = *c ; break ;
			case 4 : sd.sequence[a].base = *c ; sd.seq += *c ; break ;
			}
		}
	}
void SCFtype::fix_diff ( int mode )
	{
	signed long p_sample = 0;
	unsigned long i , num_samples = sd.tracer.size() ;
	for ( i = 0 ; i < num_samples ; i++ )
		{
		p_sample += sd.tracer[i].data[mode] ;
		sd.tracer[i].data[mode] = p_sample ;
		}
	p_sample = 0 ;
	for ( i = 0 ; i < num_samples ; i++ )
		{
		p_sample += sd.tracer[i].data[mode] ;
		sd.tracer[i].data[mode] = p_sample ;
		}
	}
 |