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/** \file
\brief Contains the EIpanel methods and the blastThread helper class
*/
#include "ExternalInterface.h"
void EIpanel::init_blast()
{
RETMAX = 10 ;
t1 = new wxTextCtrl ( up , ID_T1 , _T("") , wxDefaultPosition , wxDefaultSize , wxTE_PROCESS_ENTER ) ;
b1 = new wxButton ( up , ID_B1 , txt("b_find") , wxDefaultPosition ) ;
b2 = new wxButton ( up , ID_B2 , txt("t_open") , wxDefaultPosition ) ;
b3 = new wxButton ( up , ID_B3 , txt("b_open_link") , wxDefaultPosition ) ;
c1 = new wxChoice ( up , ID_C1 ) ;
c1->Append ( _T("Protein") ) ;
c1->Append ( _T("Nucleotide") ) ;
c2 = new wxChoice ( up , ID_C2 ) ;
c2->Append ( _T("50") ) ;
c2->Append ( _T("100") ) ;
c2->Append ( _T("250") ) ;
c2->Append ( _T("500") ) ;
b_last = new wxButton ( up , ID_B_LAST , txt("b_last") , wxDefaultPosition ) ;
b_next = new wxButton ( up , ID_B_NEXT , txt("b_next") , wxDefaultPosition ) ;
b_last->Disable () ;
b_next->Disable () ;
b3->Disable () ;
v1 = new wxBoxSizer ( wxVERTICAL ) ;
h0 = new wxBoxSizer ( wxHORIZONTAL ) ;
h0->Add ( c1 , 0 , wxEXPAND , 5 ) ;
h0->Add ( t1 , 1 , wxEXPAND , 5 ) ;
h0->Add ( b1 , 0 , wxEXPAND , 5 ) ;
h0->Add ( b2 , 0 , wxEXPAND , 5 ) ;
h0->Add ( b3 , 0 , wxEXPAND , 5 ) ;
h0->Add ( c2 , 0 , wxEXPAND , 5 ) ;
h0->Add ( b_last , 0 , wxEXPAND , 5 ) ;
h0->Add ( b_next , 0 , wxEXPAND , 5 ) ;
st_msg = new wxStaticText ( up , -1 , _T("") ) ;
v1->Add ( h0 , 0 , wxEXPAND , 0 ) ;
// v1->Add ( h1 , 0 , wxEXPAND|wxTOP|wxBOTTOM , 3 ) ;
v1->Add ( st_msg , 0 , wxEXPAND|wxTOP|wxBOTTOM , 3 ) ;
up->SetSizer ( v1 ) ;
c1->SetSelection ( 0 ) ;
c2->SetSelection ( 0 ) ;
t1->SetFocus() ;
}
#if !wxUSE_THREADS
#error "This requires thread support!"
#endif // wxUSE_THREADS
/** \brief A wxThreadHelper class to run BLAST queries in the background
*/
class blastThread : public wxThreadHelper
{
public :
/// Constructor
blastThread ( EIpanel *panel , wxString seq ) : wxThreadHelper()
{
p = panel ;
seq.Replace ( _T("|") , _T("") ) ; // Amino acid search fix
// Put
url = _T("http://www.ncbi.nlm.nih.gov/blast/Blast.cgi?") ;
url += _T("CMD=Put") ;
url += _T("&QUERY=") + seq ;
url += _T("&DATABASE=nr") ;
if ( p->c1->GetSelection() == 0 ) url += _T("&PROGRAM=blastp") ;
if ( p->c1->GetSelection() == 1 ) url += _T("&PROGRAM=blastn") ;
url += _T("&HITLIST_SIZE=") + p->c2->GetStringSelection() ;
res = ex.getText ( url ) ;
hs = parseQblast ( res ) ;
RID = hs[_T("RID")] ;
RTOE = hs[_T("RTOE")] ;
// Prepare URL
RTOE.ToLong ( &wait ) ;
url = _T("http://www.ncbi.nlm.nih.gov/blast/Blast.cgi?") ;
url += _T("CMD=Get") ;
url += _T("&RID=") + RID ;
url += _T("&FORMAT_TYPE=XML") ;
} ;
/// This display the time left, and (re-)checks online if the query is done
virtual void *Entry ()
{
// Get & wait
do {
while ( wait )
{
#ifndef __WXMAC__
wxMutexGuiEnter() ;
p->showMessage ( wxString::Format ( txt("t_blast_time") , wait ) ) ;
wxMutexGuiLeave() ;
#endif
wxThread::Sleep ( 1000 ) ; // Wait 1 sec
wait-- ;
if ( GetThread()->TestDestroy() ) // Currently, there's no way to interrupt this!
{
// MISSING : Set search button to "on" again
return NULL ;
}
}
// cout << res << endl << "----\n\n" ;
wxMutexGuiEnter() ;
res = ex.getText ( url ) ;
wxMutexGuiLeave() ;
hs = parseQblast ( res ) ;
if ( hs[_T("STATUS")].Upper() == _T("WAITING") ) wait = 5 ; // Wait another 5 seconds
else wait = 0 ; // Done!
} while ( wait ) ;
// Set result and display
p->blast_res = res ;
wxCommandEvent event( wxEVT_COMMAND_BUTTON_CLICKED, ID_B1 );
wxPostEvent ( p , event ) ;
return NULL ;
}
private :
/// I'll be damned if I remember what this is for!
wxHashString parseQblast ( wxString res )
{
wxHashString ret ;
wxString q1 = _T("QBlastInfoBegin") ;
wxString q2 = _T("QBlastInfoEnd") ;
int i ;
i = res.First ( q1 ) ;
if ( i == -1 ) return ret ;
res = res.Mid ( i + q1.Length() ) ;
i = res.First ( q2 ) ;
if ( i == -1 ) return ret ;
res = res.Left ( i - 1 ) ;
while ( !res.IsEmpty() )
{
wxString k , v ;
k = res.BeforeFirst ( '\n' ) ;
res = res.AfterFirst ( '\n' ) ;
v = k.AfterFirst ( '=' ) ;
k = k.BeforeFirst ( '=' ) ;
v = v.Trim(true).Trim(false) ;
k = k.Trim(true).Trim(false) ;
if ( v.IsEmpty() || k.IsEmpty() ) continue ;
k.MakeUpper() ;
ret[k] = v ;
}
return ret ;
}
wxString res , url , RID , RTOE ;
myExternal ex ;
wxHashString hs ;
long wait ;
EIpanel *p ;
} ;
/// This starts the thread
void EIpanel::process_blast()
{
mylog ( "blast1" , "1" ) ;
if ( !blast_res.IsEmpty() || blast_thread ) // If thread is running, or results are there...
{
mylog ( "blast1" , "1a" ) ;
process_blast2() ;
return ;
}
mylog ( "blast1" , "2" ) ;
// Start thread
blast_res.Empty() ;
wxString seq = t1->GetValue() ;
blast_thread = new blastThread ( this , seq ) ;
if ( !blast_thread || wxTHREAD_NO_ERROR != blast_thread->Create() )
{
blast_thread = NULL ;
return ;
}
mylog ( "blast1" , "3" ) ;
// blast_thread->SetPriority ( 10 ) ; // Quite low
if ( wxTHREAD_NO_ERROR != blast_thread->GetThread()->Run() )
{
blast_thread = NULL ;
return ;
}
mylog ( "blast1" , "4" ) ;
b1->Disable() ;
mylog ( "blast1" , "5" ) ;
}
/// This is called upon termination of the thread
void EIpanel::process_blast2()
{
bool initial = false ;
mylog ( "blast2" , "1" ) ;
if ( blast_thread )
{
#ifdef __WXMSW__
// blast_thread->Wait() ;
// blast_thread->Delete() ;
#endif
blast_thread = NULL ;
b1->Enable() ;
initial = true ;
res_count = 0 ;
res_start = 0 ;
}
mylog ( "blast2" , "2" ) ;
TiXmlDocument blast_doc ;
blast_doc.SetCondenseWhiteSpace(false);
blast_doc.Parse ( blast_res.mb_str() ) ;
mylog ( "blast2" , "3" ) ;
hlb->Clear () ;
showMessage ( txt("t_blast_failed") ) ;
TiXmlNode *x = blast_doc.FirstChild ( "BlastOutput" ) ;
if ( !x ) return ;
mylog ( "blast2" , "4" ) ;
int w , h ;
hlb->GetClientSize ( &w , &h ) ;
w /= 8 ;
wxString blast_version = valFC ( x->FirstChild ( "BlastOutput_version" ) ) ;
x = x->FirstChild ( "BlastOutput_iterations" ) ;
x = x->FirstChild ( "Iteration" ) ;
x = x->FirstChild ( "Iteration_hits" ) ;
mylog ( "blast2" , "5" ) ;
int a = 0 ;
for ( x = x->FirstChild ( "Hit" ) ; x ; x = x->NextSibling ( "Hit" ) , a++ )
{
if ( initial )
{
res_count++ ;
if ( a >= RETMAX ) continue ;
}
else
{
if ( a < res_start ) continue ;
if ( a >= res_start + RETMAX ) break ;
}
wxString html ;
wxString name = valFC ( x->FirstChild ( "Hit_def" ) ) ;
wxString id = valFC ( x->FirstChild ( "Hit_id" ) ) ;
name = name.BeforeFirst ( '>' ) ;
TiXmlNode *h = x->FirstChild ( "Hit_hsps" ) ;
h = h->FirstChild ( "Hsp" ) ;
wxString evalue = valFC ( h->FirstChild ( "Hsp_evalue" ) ) ;
if ( evalue.Find ( 'e' ) > -1 )
{
wxString base = evalue.BeforeFirst ( 'e' ) + _T("×10") ;
wxString exp = _T("<font size=2>") + evalue.AfterFirst ( 'e' ) + _T("</font>") ;
evalue = _T("<table border=0 cellpadding=0 cellspacing=0><tr><td rowspan=2 valign=bottom>E-Value=</td>") ;
evalue += _T("<td align=left valign=bottom><br>") + base + _T("</td>") ;
evalue += _T("<td align=right valign=top>") + exp + _T("</td>") ;
evalue += _T("</tr></table>") ;
}
else evalue = _T("E-Value=") + evalue ;
wxString qseq = valFC ( h->FirstChild ( "Hsp_qseq" ) ) ;
wxString mseq = valFC ( h->FirstChild ( "Hsp_midline" ) ) ;
wxString hseq = valFC ( h->FirstChild ( "Hsp_hseq" ) ) ;
long qoff , hoff ;
valFC ( h->FirstChild ( "Hsp_query-from" ) ) . ToLong ( &qoff ) ;
valFC ( h->FirstChild ( "Hsp_hit-from" ) ) . ToLong ( &hoff ) ;
html = _T("<table width=100%><tr>") ;
html += _T("<td rowspan=2>") + wxString::Format ( _T("%d") , a+1 ) + _T("</td>") ;
html += _T("<td valign=top width=100%>") + name + _T("</td>") ;
html += _T("<td align=right valign=top>") + evalue + _T("</td>") ;
html += _T("</tr><tr>") ;
html += _T("<td colspan=2><tt><font size=2>\n") ;
html += blast_align ( qseq , mseq , hseq , w , qoff , hoff ) ;
html += _T("</font></tt></td>") ;
html += _T("</tr></table>") ;
hlb->Set ( a - res_start , html , id ) ;
}
mylog ( "blast2" , "6" ) ;
hlb->Update () ;
mylog ( "blast2" , "7" ) ;
// wxMessageBox ( wxString::Format ( "%d" , res_count ) ) ;
b_next->Enable ( res_start + RETMAX < res_count ) ;
b_last->Enable ( res_start > 0 ) ;
b3->Enable ( true ) ;
int max = res_start + RETMAX ;
if ( max > res_count ) max = res_count ;
showMessage ( wxString::Format ( txt("t_blast_results_by" ) , blast_version.c_str() , res_start+1 , max , res_count ) ) ;
wxWakeUpIdle();
mylog ( "blast2" , "8" ) ;
}
wxString EIpanel::blast_align ( wxString qseq , wxString mseq , wxString hseq , int cpl , int qoff , int hoff )
{
wxString lead[3] ;
lead[0] = txt("t_blast_qseq" ) ;
lead[2] = txt("t_blast_hseq" ) ;
while ( lead[0].Length() < lead[2].Length() ) lead[0] += _T(" ") ;
while ( lead[0].Length() > lead[2].Length() ) lead[2] += _T(" ") ;
lead[1].Append ( ' ' , lead[0].Length() ) ;
cpl -= lead[0].Length() + 1 ;
qoff-- ;
hoff-- ;
// Unify length
int max = qseq.Length() ;
max = max < mseq.Length() ? mseq.Length() : max ;
max = max < hseq.Length() ? hseq.Length() : max ;
while ( max % cpl ) max++ ;
qseq.Append ( ' ' , max - qseq.Length() ) ;
mseq.Append ( ' ' , max - mseq.Length() ) ;
hseq.Append ( ' ' , max - hseq.Length() ) ;
wxString ret ;
while ( !qseq.IsEmpty() )
{
// ret += "<p>" ;
int a ;
wxString z ;
for ( a = 0 , z = qseq.Left ( cpl ) ; a < z.length() ; a++ ) if ( z.GetChar(a) >= 'A' && z.GetChar(a) <= 'Z' ) qoff++ ;
for ( a = 0 , z = hseq.Left ( cpl ) ; a < z.length() ; a++ ) if ( z.GetChar(a) >= 'A' && z.GetChar(a) <= 'Z' ) hoff++ ;
ret += lead[0] + _T(" ") + qseq.Left ( cpl ) + _T(" ") + wxString::Format ( _T("%5d") , qoff ) + _T("\n") ;
ret += lead[1] + _T(" ") + mseq.Left ( cpl ) + _T("\n") ;
ret += lead[2] + _T(" ") + hseq.Left ( cpl ) + _T(" ") + wxString::Format ( _T("%5d") , hoff ) + _T("\n") ;
qseq = qseq.Mid ( cpl ) ;
mseq = mseq.Mid ( cpl ) ;
hseq = hseq.Mid ( cpl ) ;
if ( !qseq.IsEmpty() ) ret += _T("<p></p>\n") ;
}
ret.Replace ( _T(" ") , _T(" ") ) ;
return ret ;
}
void EIpanel::execute_blast()
{
wxString database = c1->GetStringSelection() ;
execute_ncbi_load ( database ) ; // Dummy
}
void EIpanel::execute_blast_b3()
{
wxString database = c1->GetStringSelection().Lower() ;
for ( int i = 0 ; i < hlb->data.GetCount() ; i++ )
{
if ( !hlb->IsSelected ( i ) ) continue ;
wxString s = hlb->data[i] ;
int a = s.Find ( _T("gi|") ) ;
if ( a == -1 ) return ;
s = s.Mid ( a + 3 ) ;
s = s.BeforeFirst ( '|' ) ;
s = _T("http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?cmd=Retrieve&db=") + database + _T("&list_uids=") + s + _T("&dopt=GenPept") ;
s = myapp()->getHTMLCommand ( s ) ;
wxExecute ( s ) ;
}
}
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