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|
##################################################
#
# README mhcregion
#
# This README runs as a shell script.
# Here you can find the commands
# we used to produce all the figures for:
#
# "Analysis of a Syntenic Region by SIM"
#
# USAGE: bash README > .log 2>&1
#
# $Id: README,v 1.1 2003/03/04 18:06:17 jabril Exp $
#
##################################################
#
SECONDS=0 ;
#
#
# Original Commands:
#
# 1/ producing the left panel with genbank annotation, using tblastx alignment:
#
# gff2aplot19 -v -L Identity% -l "LNMP" -T "GenBank Annotation" h.tag.gff m.tag.gff h.fasta_m.fasta.tblastx h.fast_m.fasta.lnmp.gff > h_m.t.tblastx.ps
#
# 2/ producing the left panel with genbank annotation, using sim alignment:
#
# gff2aplot19 -v -L Identity% -T GenBank_Annotation h.tag.gff m.tag.gff h_m.sim.gf > h_m.t.ps
#
# producing the right panel with genbank annotation+prediction, using sim alignment:
# gff2aplot19 -v -l ESTs -L Identity% -X Human -Y Mouse -Z -E 6500 -e 6500 -T "Comparison of prediction and annotation" h.tag.gff m.tag.gff h_m.sim.gff h_m.pred.gff h_est.gff > h_m.z.ps
#
# Fixing GFF input files
#
# gawk '{$2="genbank"; print $0}' hs.gff.tmp > hs.gff
# gawk '{$2="genbank"; print $0}' mm.gff.tmp > mm.gff
#
# Getting score limits
#
# gawk 'BEGIN {min=max="kk"} $3~/fragment/ { if (min=="kk") { min=max=$6 } else { if (min>$6) { min=$6; next }; if (max<$6) max=$6}} END { print "MIN "min" : MAX "max}' hs-mm.sim.gff
# MIN 0.380 : MAX 1.000
#
# gawk 'BEGIN {min=max="kk"} $3~/alignment/ { if (min=="kk") { min=max=$6 } else { if (min>$6) { min=$6; next }; if (max<$6) max=$6}} END { print "MIN "min" : MAX "max}' hs-mm.sim.gff
# MIN 208.000 : MAX 18340.000
#
echo "# Running ALI2GFF" ;
#
$BIN/ali2gff -x "X87344" \
-y "AF100956+AF027865" \
hs-mm.sim > hs-mm.sim.gff ;
perl -npe 's{/home.+/(ali2gff)}{ $1};' hs-mm.sim.gff > tmp.gff ;
mv -v tmp.gff hs-mm.sim.gff ;
#
echo "# Getting the PostScripts with GFF2APLOT" ;
#
echo "# -> Default plot" ;
#
$BIN/gff2aplot.pl \
--verbose \
--percent-box-label "Identity%" \
--title "Human/Mouse MHCII Region" \
--subtitle "" \
-- hs.gff \
mm.gff \
hs-mm.sim.gff \
> hs-mm.sim.ps \
2> hs-mm.sim.log ;
#
echo "# -> CMDline settings" ;
#
$BIN/gff2aplot.pl \
--verbose \
--percent-box-label "Identity%" \
--show-percent-box \
--title "Human/Mouse MHCII Region" \
--subtitle "" \
--feature-var "seqbounds::alignment_color=grey" \
--feature-var "alignment::alignment_color=red" \
--feature-var "fragment::alignment_color=green" \
--strand-var "..::strand_layer=0" \
--strand-var "++::strand_layer=2" \
-- hs.gff \
mm.gff \
hs-mm.sim.gff \
> hs-mm.sim2.ps \
2> hs-mm.sim2.log ;
#
# in case the strand for seqbounds was same as the other features:
#
# --feature-var "seqbounds::alignment_color=grey" \
# --feature-var "seqbounds::feature_layer=0" \
# --feature-var "alignment::alignment_color=red" \
# --feature-var "alignment::feature_layer=1" \
# --feature-var "fragment::alignment_color=green" \
# --feature-var "fragment::feature_layer=2" \
#
# Extra customization
cat > mhc2.rc <<'+++EOF+++'
#
# L #
show_percent_box=on
aplot_score_range=0..1
# percent_box_score_range=0..1
major_tickmark_score=0.25
minor_tickmark_score=0.05
zoom_area_mark_color=violet
zoom_area_fill_color=paleviolet
#
# G #
*::group_shape=arrow
/.*polyA.*/::group_shape=none
/.*polyA.*/::show_group_label=off
#
# F #
*::ribbon_style=ribbons
seqbounds::hide=on
alignment::hide=on
fragment::alignment_scale_color=on
fragment::feature_color=black
3'-utr::feature_color=grey
3'-utr::show_ribbons=off
polya::feature_color=grey
polya::show_ribbons=off
First::feature_color=lightgreen
First::show_ribbons=on
First::ribbon_color=palegreen
Internal::feature_color=lightgreen
Internal::show_ribbons=on
Internal::ribbon_color=palegreen
Terminal::feature_color=lightgreen
Terminal::show_ribbons=on
Terminal::ribbon_color=palegreen
+++EOF+++
#
cat > mhc2spc.rc <<'+++EOF+++'
#
# L #
alignment::hide=off
alignment::feature_color=lightorange
+++EOF+++
#
echo "# -> Customized settings" ;
#
$BIN/gff2aplot.pl \
--verbose \
--percent-box-label "Identity%" \
--show-percent-box \
--title "Human/Mouse MHCII Region" \
--subtitle "" \
--x-label "Human" --y-label "Mouse" \
--custom-filename mhc2.rc \
--custom-filename mhc2spc.rc \
-- hs.gff \
mm.gff \
hs-mm.sim.gff \
> hs-mm.sim.ini.ps \
2> hs-mm.sim.ini.log ;
#
### ZOOM MARKED
#
cat > mhc2labels.rc <<'+++EOF+++'
#
# L #
group_x_label_angle=45
group_y_label_angle=315
+++EOF+++
#
#
echo "# -> MARKING ZOOM BOX" ;
#
$BIN/gff2aplot.pl \
--verbose \
--percent-box-label "Identity%" \
--show-percent-box \
--title "Human/Mouse MHCII Region" \
--subtitle "Zooming into the LMP2 gene region" \
--zoom-area \
--end-x-sequence 6500 \
--end-y-sequence 6500 \
--x-label "Human X87344" \
--y-label "Mouse AF100956+AF027865" \
--custom-filename mhc2.rc \
--custom-filename mhc2labels.rc \
-- hs.gff \
mm.gff \
hs-mm.sim.gff \
> hs-mm.sim.zbox.ps \
2> hs-mm.sim.zbox.log ;
#
### ZOOMED
#
cat > mhc2+sgp1.rc <<'+++EOF+++'
#
# L #
zoom_marks=on
aplot_box_bgcolor=verypaleviolet
percent_box_bgcolor=verypaleviolet
percent_box_score_range=0.2..1.0
major_tickmark_score=0.2
minor_tickmark_score=0.05
#
# S #
sgp::source_layer=1
genbank::source_layer=2
#
# G #
/.*polyA.*/::show_group_label=on
/.*polyA.*/::group_shape=bracket
/.*polyA.*/::show_group_limits=on
/.*Gene.*/::show_group_label=off
/.*Gene.*/::group_shape=none
#
# F #
/^p(First|Internal|Terminal)$/::feature_color=red
/^p(First|Internal|Terminal)$/::feature_shape=box
/^p(First|Internal|Terminal)$/::show_ribbons=on
/^p(First|Internal|Terminal)$/::ribbon_color=verylightred
/^(First|Internal|Terminal)$/::feature_color=lightgreen
/^(polya|3'-utr|First|Internal|Terminal)$/::feature_shape=half_box
/^(First|Internal|Terminal)$/::ribbon_color=limegreen
3'-utr::show_ribbons=on
3'-utr::ribbon_color=grey
polya::show_ribbons=on
polya::ribbon_color=grey
+++EOF+++
#
echo "# -> ZOOMED REGION" ;
#
$BIN/gff2aplot.pl \
--verbose \
--percent-box-label "Identity%" \
--show-percent-box \
--title "Human/Mouse MHCII Region" \
--subtitle "Zooming into the LMP2 gene region" \
--zoom \
--end-x-sequence 6500 \
--end-y-sequence 6500 \
--x-label "Human" \
--y-label "Mouse" \
--custom-filename mhc2.rc \
--custom-filename mhc2labels.rc \
--custom-filename mhc2+sgp1.rc \
-- hs.gff \
mm.gff \
hs-mm.sgp1.gff \
hs-mm.sim.gff \
> hs-mm.sim.zoom.ps \
2> hs-mm.sim.zoom.log ;
#
#
# perl -e '$x="X87344"; $y="AF100956+AF027865"; $a="X87344:AF100956+AF027865"; $x = &escape_input($x); $y = &escape_input($y); $a = &escape_input($a); @b=split /:/o, $a,2; print "$b[0] : $b[1] ::: $b[0] =~ $x -> ".($b[0] =~ /^$x$/)." ::: $b[0] eq $x -> ".($b[0] eq $x)." ::: \"$b[0]\" eq \"$x\" -> ".("$b[0]" eq "$x")."\n"; print "$b[0] : $b[1] ::: $b[1] =~ $y -> ".($b[1] =~ /^$y$/)." ::: $b[1] eq $y -> ".($b[1] eq $y)." ::: \"$b[1]\" eq \"$y\" -> ".("$b[1]" eq "$y")."\n"; sub escape_input() { my $var = $_[0]; $var =~ s{([+*;,<>&!\{\}`'\''"])}{\\$1}g; return $var;}'
#
#
# Preparing web images
#
filenames () {
cat << 'EOF';
hs-mm.sim
hs-mm.sim2
hs-mm.sim.ini
hs-mm.sim.zbox
hs-mm.sim.zoom
EOF
};
#
# echo "# Using \"ghostscript\" to obtain JPEG images from PS files" ;
echo "# Using \"ghostscript\" to obtain PNG images from PS files" ;
#
GS=`which gs || echo "gs"`;
MG=`which mogrify || echo "mogrify"`;
CV=`which convert || echo "convert"`;
#
filenames | while read n ;
do {
echo "# -> $n" ;
$GS -dBATCH -dNOPAUSE -r300 \
-sPAPERSIZE=a4 -sDEVICE=png16m \
-sOutputFile=$n.png $n.ps ;
cp -v $n.png $n.s.png ;
# mogrify is a ImageMagick tool that allow us to transform images on batch,
# large high-resolution jpeg files are scaled down to thumbnail resolution (2479x3508=>123x175)
# it overwrites original file (so that we previously get a renamed copy for each jpeg to process).
$MG -verbose -geometry 5% $n.s.png ;
#
# $GS -dBATCH -dNOPAUSE -r300 \
# -sPAPERSIZE=a4 -sDEVICE=jpeg \
# -sOutputFile=$n.jpg $n.ps ;
# png large version is pretty smaller than jpeg
# cp -v $n.jpg $n.s.jpg ;
# $MG -verbose -geometry 5% $n.s.jpg ;
};
done ;
$CV 'vid:*.s.png' mhcregion.summary.png
#
echo "# Using \"ghostscript\" to obtain PDF documents from PS files" ;
#
filenames | while read n ;
do {
echo "# -> $n" ;
$GS -dBATCH -dNOPAUSE -r300 \
-sPAPERSIZE=a4 -sDEVICE=pdfwrite \
-sOutputFile=$n.pdf $n.ps ;
};
done ;
#
#
# echo "# LaTeXing all figures together..." ;
#
# latex mhcregion.tex;
# dvips mhcregion.dvi -o mhcregion.ps;
#
#
echo "###" ;
echo "### TOTAL TIME: $SECONDS seconds..." ;
#
#
# That's all folks... ;^D
#
# >> Copyleft (C) 2002/2003 - Josep F. Abril <<
#
|
