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// Copyright ©2011-2013 The bíogo Authors. All rights reserved.
// Use of this source code is governed by a BSD-style
// license that can be found in the LICENSE file.
// Package alphabet describes biological sequence letters, including quality scores.
package alphabet
import (
"github.com/biogo/biogo/feat"
"errors"
"fmt"
"strings"
"unicode"
)
const (
CaseSensitive = true
)
// Package alphabet provides default Alphabets for DNA, RNA and Protein. These
// alphabets are case insensitive and for the non-redundant nucleic acid alphabets
// satisfy the condition that the index of a letter is equal to the bitwise-complement
// of the index of the base-complement, modulo 4.
var (
DNA = MustComplement(NewComplementor(
"acgt",
feat.DNA,
MustPair(NewPairing("acgtnxACGTNX-", "tgcanxTGCANX-")),
'-', 'n',
!CaseSensitive,
))
DNAgapped = MustComplement(NewComplementor(
"-acgt",
feat.DNA,
MustPair(NewPairing("acgtnxACGTNX-", "tgcanxTGCANX-")),
'-', 'n',
!CaseSensitive,
))
DNAredundant = MustComplement(NewComplementor(
"-acmgrsvtwyhkdbn",
feat.DNA,
MustPair(NewPairing("acmgrsvtwyhkdbnxACMGRSVTWYHKDBNX-", "tgkcysbawrdmhvnxTGKCYSBAWRDMHVNX-")),
'-', 'n',
!CaseSensitive,
))
RNA = MustComplement(NewComplementor(
"acgu",
feat.RNA,
MustPair(NewPairing("acgunxACGUNX-", "ugcanxUGCANX-")),
'-', 'n',
!CaseSensitive,
))
RNAgapped = MustComplement(NewComplementor(
"-acgu",
feat.RNA,
MustPair(NewPairing("acgunxACGUNX-", "ugcanxUGCANX-")),
'-', 'n',
!CaseSensitive,
))
RNAredundant = MustComplement(NewComplementor(
"-acmgrsvuwyhkdbn",
feat.RNA,
MustPair(NewPairing("acmgrsvuwyhkdbnxACMGRSVUWYHKDBNX-", "ugkcysbawrdmhvnxUGKCYSBAWRDMHVNX-")),
'-', 'n',
!CaseSensitive,
))
Protein = Must(NewAlphabet(
"-abcdefghijklmnpqrstvwxyz*",
feat.Protein,
'-', 'x',
!CaseSensitive,
))
)
// Must is a helper that wraps a call to a function returning (Alphabet, error)
// and panics if the error is non-nil. It is intended for use in variable
// initializations.
func Must(a Alphabet, err error) Alphabet {
if err != nil {
panic(err)
}
return a
}
// MustComplement is a helper that wraps a call to a function returning (Complementor, error)
// and panics if the error is non-nil. It is intended for use in variable
// initializations.
func MustComplement(c Complementor, err error) Complementor {
if err != nil {
panic(err)
}
return c
}
// MustPair is a helper that wraps a call to a function returning (*Pairing, error)
// and panics if the error is non-nil. It is intended for use in variable
// initializations.
func MustPair(p *Pairing, err error) *Pairing {
if err != nil {
panic(err)
}
return p
}
// Type Index is a pointer to an index table.
type Index *[256]int
// An Alphabet describes valid single character letters within a sequence.
type Alphabet interface {
// IsValid reports whether a letter conforms to the alphabet.
IsValid(Letter) bool
// AllValid reports whether a slice of bytes conforms to the alphabet.
// It returns the index of the first invalid byte,
// or a negative int if all bytes are valid.
AllValid([]Letter) (ok bool, pos int)
// AllValidQLetter reports whether a slice of bytes conforms to the alphabet.
// It returns the index of the first invalid byte,
// or a negative int if all bytes are valid.
AllValidQLetter([]QLetter) (ok bool, pos int)
// Len returns the number of distinct valid letters in the alphabet.
Len() int
// IndexOf returns the index of a given letter.
IndexOf(Letter) int
// Letter returns the letter corresponding to the given index.
Letter(int) Letter
// LetterIndex returns a pointer to the internal array specifying
// letter to index conversion. The returned index should not be altered.
LetterIndex() Index
// Letters returns a string of letters conforming to the alphabet in index
// order. In case insensitive alphabets, both cases are presented.
Letters() string
// ValidLetters returns a slice of the internal []bool indicating valid
// letters. The returned slice should not be altered.
ValidLetters() []bool
// Gap returns the gap character used by the alphabet.
Gap() Letter
// Ambiguous returns the character representing an ambiguous letter.
Ambiguous() Letter
// Moltype returns the molecule type of the alphabet.
Moltype() feat.Moltype
// IsCased returns whether the alphabet is case sensitive.
IsCased() bool
}
// A Complementor is an Alphabet that describes the complementation relationships
// between letters.
type Complementor interface {
Alphabet
Complement(Letter) (Letter, bool)
ComplementTable() []Letter
}
// Single letter alphabet type.
type alpha struct {
letters string
length int
valid [256]bool
index [256]int
gap, ambiguous Letter
caseSensitive bool
molType feat.Moltype
}
func newAlphabet(letters string, molType feat.Moltype, gap, ambiguous Letter, caseSensitive bool) (*alpha, error) {
if strings.IndexFunc(letters, func(r rune) bool { return r < 0 || r > unicode.MaxASCII }) > -1 {
return nil, errors.New("alphabet: letters contains non-ASCII rune")
}
a := &alpha{
length: len(letters),
gap: gap,
ambiguous: ambiguous,
caseSensitive: caseSensitive,
molType: molType,
}
for i := range a.index {
a.index[i] = -1
}
if caseSensitive {
a.letters = letters
for i, l := range a.letters {
a.valid[l] = true
a.index[l] = i
}
return a, nil
}
a.letters = strings.ToLower(letters) + strings.ToUpper(letters)
for i, l := range a.letters[:len(letters)] {
a.valid[l] = true
a.index[l] = i
}
for i, l := range a.letters[len(letters):] {
a.valid[l] = true
a.index[l] = a.index[a.letters[i]]
}
return a, nil
}
func (a *alpha) Moltype() feat.Moltype { return a.molType }
func (a *alpha) Len() int { return a.length }
func (a *alpha) IsCased() bool { return a.caseSensitive }
func (a *alpha) Gap() Letter { return a.gap }
func (a *alpha) Ambiguous() Letter { return a.ambiguous }
func (a *alpha) AllValidQLetter(n []QLetter) (bool, int) {
for i, v := range n {
if !a.valid[v.L] {
return false, i
}
}
return true, -1
}
func (a *alpha) AllValid(n []Letter) (bool, int) {
for i, v := range n {
if !a.valid[v] {
return false, i
}
}
return true, -1
}
func (a *alpha) IsValid(n Letter) bool {
return a.valid[n]
}
func (a *alpha) Letter(i int) Letter {
return Letter(a.letters[:a.length][i])
}
func (a *alpha) IndexOf(n Letter) int {
return a.index[n]
}
func (a *alpha) ValidLetters() []bool { return a.valid[:] }
func (a *alpha) LetterIndex() Index { return Index(&a.index) }
func (a *alpha) Letters() string { return a.letters }
// A Pairing provides a lookup table between a letter and its complement.
type Pairing struct {
pair []Letter
ok []bool
complements [256]Letter
}
// NewPairing create a new Pairing from a pair of strings. Pairing definitions must be
// a bijection and must contain only ASCII characters.
func NewPairing(s, c string) (*Pairing, error) {
if len(s) != len(c) {
return nil, errors.New("alphabet: length of pairing definitions do not match")
}
p := &Pairing{
pair: make([]Letter, 256),
ok: make([]bool, 256),
}
for i := range p.pair {
p.pair[i] = Letter(i)
}
cr := []rune(c)
for i, v := range s {
if v < 0 || cr[i] < 0 || v > unicode.MaxASCII || cr[i] > unicode.MaxASCII {
return nil, errors.New("alphabet: pairing definition contains non-ASCII rune")
}
p.pair[v] = Letter(cr[i])
p.ok[v] = true
}
for i, l := range s {
if Letter(l) != p.pair[p.pair[l]] {
return nil, errors.New("alphabet: pairing definition is not a bijection")
}
if Letter(c[i]) != p.pair[p.pair[c[i]]] {
return nil, errors.New("alphabet: pairing definition is not a bijection")
}
}
copy(p.complements[:], p.pair)
for i, ok := range p.ok {
if !ok {
p.complements[i] |= unicode.MaxASCII + 1
}
}
return p, nil
}
// Returns the complement of a letter and true if the complement is a valid letter otherwise unchanged and false.
func (p *Pairing) Complement(l Letter) (c Letter, ok bool) { return p.pair[l], p.ok[l] }
// Returns a complementation table based on the internal representation. Invalid pairs hold a value outside the ASCII range.
// The caller must not modify the returned table.
func (p *Pairing) ComplementTable() []Letter {
return p.complements[:]
}
type nucleic struct {
*alpha
*Pairing
}
// NewComplementor returns a complementing alphabet. The Complement table is checked for
// validity and an error is returned if an invalid complement pair is found. Pairings
// that result in no change but would otherwise be invalid are allowed. Letter parameter
// handling is the same as for NewAlphabet.
func NewComplementor(letters string, molType feat.Moltype, pairs *Pairing, gap, ambiguous Letter, caseSensitive bool) (Complementor, error) {
a, err := newAlphabet(letters, molType, gap, ambiguous, caseSensitive)
if err != nil {
return nil, err
}
if pairs != nil {
for i, v := range pairs.pair {
if !(pairs.ok[i] || Letter(i&unicode.MaxASCII) == v&unicode.MaxASCII) && !(a.valid[i] && a.valid[v]) {
return nil, fmt.Errorf("alphabet: invalid pairing: %c (%d) -> %c (%d)", i, i, v, v)
}
}
}
return &nucleic{
alpha: a,
Pairing: pairs,
}, nil
}
// NewAlphabet returns a new Alphabet based on the provided definitions. Index values
// for letters reflect order of the letters parameter. Letters must be within the
// ASCII range. No check is performed to determine whether letters appear more than once,
// the index of a letter will be the position of the last occurrence of that letter in the
// letters parameter.
func NewAlphabet(letters string, molType feat.Moltype, gap, ambiguous Letter, caseSensitive bool) (Alphabet, error) {
return newAlphabet(letters, molType, gap, ambiguous, caseSensitive)
}
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