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// Copyright ©2014 The bíogo Authors. All rights reserved.
// Use of this source code is governed by a BSD-style
// license that can be found in the LICENSE file.
// Package tabix implements tabix coordinate sorted indexing.
package tabix
import (
"encoding/binary"
"errors"
"fmt"
"io"
"strings"
"github.com/biogo/hts/bgzf"
"github.com/biogo/hts/bgzf/index"
"github.com/biogo/hts/internal"
)
// Index is a tabix index.
type Index struct {
Format byte
ZeroBased bool
NameColumn int32
BeginColumn int32
EndColumn int32
MetaChar rune
Skip int32
refNames []string
nameMap map[string]int
idx internal.Index
}
// New returns a new tabix index.
func New() *Index {
return &Index{nameMap: make(map[string]int)}
}
// NumRefs returns the number of references in the index.
func (i *Index) NumRefs() int {
return len(i.idx.Refs)
}
// Names returns the reference names in the index. The returned
// slice should not be altered.
func (i *Index) Names() []string {
return i.refNames
}
// IDs returns a map of strings to integer IDs. The returned
// map should not be altered.
func (i *Index) IDs() map[string]int {
return i.nameMap
}
// ReferenceStats returns the index statistics for the given reference and true
// if the statistics are valid.
func (i *Index) ReferenceStats(id int) (stats index.ReferenceStats, ok bool) {
s := i.idx.Refs[id].Stats
if s == nil {
return index.ReferenceStats{}, false
}
return index.ReferenceStats(*s), true
}
// Unmapped returns the number of unmapped reads and true if the count is valid.
func (i *Index) Unmapped() (n uint64, ok bool) {
if i.idx.Unmapped == nil {
return 0, false
}
return *i.idx.Unmapped, true
}
// Record wraps types that may be indexed by an Index.
type Record interface {
RefName() string
Start() int
End() int
}
type tabixShim struct {
id, start, end int
}
func (r tabixShim) RefID() int { return r.id }
func (r tabixShim) Start() int { return r.start }
func (r tabixShim) End() int { return r.end }
// Add records the SAM record as having being located at the given chunk.
func (i *Index) Add(r Record, c bgzf.Chunk, placed, mapped bool) error {
refName := r.RefName()
rid, ok := i.nameMap[refName]
if !ok {
rid = len(i.refNames)
i.refNames = append(i.refNames, refName)
}
shim := tabixShim{id: rid, start: r.Start(), end: r.End()}
return i.idx.Add(shim, internal.BinFor(r.Start(), r.End()), c, placed, mapped)
}
// Chunks returns a []bgzf.Chunk that corresponds to the given genomic interval.
func (i *Index) Chunks(ref string, beg, end int) ([]bgzf.Chunk, error) {
id, ok := i.nameMap[ref]
if !ok {
return nil, index.ErrNoReference
}
chunks, err := i.idx.Chunks(id, beg, end)
if err != nil {
return nil, err
}
return adjacent(chunks), nil
}
var adjacent = index.Adjacent
// MergeChunks applies the given MergeStrategy to all bins in the Index.
func (i *Index) MergeChunks(s index.MergeStrategy) {
i.idx.MergeChunks(s)
}
var tbiMagic = [4]byte{'T', 'B', 'I', 0x1}
// ReadFrom reads the tabix index from the given io.Reader. Note that
// the tabix specification states that the index is stored as BGZF, but
// ReadFrom does not perform decompression.
func ReadFrom(r io.Reader) (*Index, error) {
var (
idx Index
magic [4]byte
err error
)
err = binary.Read(r, binary.LittleEndian, &magic)
if err != nil {
return nil, err
}
if magic != tbiMagic {
return nil, errors.New("tabix: magic number mismatch")
}
var n int32
err = binary.Read(r, binary.LittleEndian, &n)
if err != nil {
return nil, err
}
if n == 0 {
return nil, nil
}
err = readTabixHeader(r, &idx)
if err != nil {
return nil, err
}
if len(idx.refNames) != int(n) {
return nil, fmt.Errorf("tabix: name count mismatch: %d != %d", len(idx.refNames), n)
}
idx.nameMap = make(map[string]int)
for i, n := range idx.refNames {
idx.nameMap[n] = i
}
idx.idx, err = internal.ReadIndex(r, n, "tabix")
if err != nil {
return nil, err
}
return &idx, nil
}
func readTabixHeader(r io.Reader, idx *Index) error {
var (
format int32
err error
)
err = binary.Read(r, binary.LittleEndian, &format)
if err != nil {
return fmt.Errorf("tabix: failed to read format: %v", err)
}
idx.Format = byte(format)
idx.ZeroBased = format&0x10000 != 0
err = binary.Read(r, binary.LittleEndian, &idx.NameColumn)
if err != nil {
return fmt.Errorf("tabix: failed to read name column index: %v", err)
}
err = binary.Read(r, binary.LittleEndian, &idx.BeginColumn)
if err != nil {
return fmt.Errorf("tabix: failed to read begin column index: %v", err)
}
err = binary.Read(r, binary.LittleEndian, &idx.EndColumn)
if err != nil {
return fmt.Errorf("tabix: failed to read end column index: %v", err)
}
err = binary.Read(r, binary.LittleEndian, &idx.MetaChar)
if err != nil {
return fmt.Errorf("tabix: failed to read metacharacter: %v", err)
}
err = binary.Read(r, binary.LittleEndian, &idx.Skip)
if err != nil {
return fmt.Errorf("tabix: failed to read skip count: %v", err)
}
var n int32
err = binary.Read(r, binary.LittleEndian, &n)
if err != nil {
return fmt.Errorf("tabix: failed to read name lengths: %v", err)
}
nameBytes := make([]byte, n)
_, err = io.ReadFull(r, nameBytes)
if err != nil {
return fmt.Errorf("tabix: failed to read names: %v", err)
}
names := string(nameBytes)
if names[len(names)-1] != 0 {
return errors.New("tabix: last name not zero-terminated")
}
idx.refNames = strings.Split(names[:len(names)-1], string(0))
return nil
}
// WriteTo writes the index to the given io.Writer. Note that
// the tabix specification states that the index is stored as BGZF, but
// WriteTo does not perform compression.
func WriteTo(w io.Writer, idx *Index) error {
err := binary.Write(w, binary.LittleEndian, tbiMagic)
if err != nil {
return err
}
err = binary.Write(w, binary.LittleEndian, int32(len(idx.idx.Refs)))
if err != nil {
return err
}
err = writeTabixHeader(w, idx)
if err != nil {
return err
}
return internal.WriteIndex(w, &idx.idx, "tabix")
}
func writeTabixHeader(w io.Writer, idx *Index) error {
var err error
format := int32(idx.Format)
if idx.ZeroBased {
format |= 0x10000
}
err = binary.Write(w, binary.LittleEndian, format)
if err != nil {
return fmt.Errorf("tabix: failed to write format: %v", err)
}
err = binary.Write(w, binary.LittleEndian, idx.NameColumn)
if err != nil {
return fmt.Errorf("tabix: failed to write name column index: %v", err)
}
err = binary.Write(w, binary.LittleEndian, idx.BeginColumn)
if err != nil {
return fmt.Errorf("tabix: failed to write begin column index: %v", err)
}
err = binary.Write(w, binary.LittleEndian, idx.EndColumn)
if err != nil {
return fmt.Errorf("tabix: failed to write end column index: %v", err)
}
err = binary.Write(w, binary.LittleEndian, idx.MetaChar)
if err != nil {
return fmt.Errorf("tabix: failed to write metacharacter: %v", err)
}
err = binary.Write(w, binary.LittleEndian, idx.Skip)
if err != nil {
return fmt.Errorf("tabix: failed to write skip count: %v", err)
}
var n int32
for _, name := range idx.refNames {
n += int32(len(name) + 1)
}
err = binary.Write(w, binary.LittleEndian, n)
if err != nil {
return fmt.Errorf("tabix: failed to write name lengths: %v", err)
}
for _, name := range idx.refNames {
_, err = w.Write([]byte(name))
if err != nil {
return fmt.Errorf("tabix: failed to write name: %v", err)
}
_, err = w.Write([]byte{0})
if err != nil {
return fmt.Errorf("tabix: failed to write name: %v", err)
}
}
return nil
}
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